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axioobserver d1 inverted fluorescence microscope  (Carl Zeiss)


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    Carl Zeiss axioobserver d1 inverted fluorescence microscope
    Axioobserver D1 Inverted Fluorescence Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 99/100, based on 4893 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/axioobserver%2Ed1+microscope/Inverted+microscope+Axio+Observer+3/pmc04255936-167-8-7
    Average 99 stars, based on 4893 article reviews
    axioobserver d1 inverted fluorescence microscope - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Interaction between FmRAV4 (a RAV transcription factor) and FmMYB1 (a MYB transcription factor) confers drought tolerance via ROS scavenging in Fraxinus mandshurica.
    Article Snippet: Drought stress severely restricts plant growth and development.. Although AP2/ERF family transcription factors (TFs) participate extensively in abiotic stress responses, they remain largely unexplored in Fraxinus mandshurica.. In this study, we identified and functionally characterized FmRAV4, a TF from the RAV subfamily.

    Staining:

    Article Title: Interaction between FmRAV4 (a RAV transcription factor) and FmMYB1 (a MYB transcription factor) confers drought tolerance via ROS scavenging in Fraxinus mandshurica.
    Article Snippet: Drought stress severely restricts plant growth and development.. Although AP2/ERF family transcription factors (TFs) participate extensively in abiotic stress responses, they remain largely unexplored in Fraxinus mandshurica.. In this study, we identified and functionally characterized FmRAV4, a TF from the RAV subfamily.

    Article Title: Photoinduced hydrogel coatings hybridized with silver nanoparticles to prevent Nitratidesulfovibrio vulgaris adhesion and bio-corrosion of aluminum substrates
    Article Snippet: At the start of the experiment, the optical density at 600 nm was 0.014, measured using a Spectronic 401 (Spectronic Instruments, Milton Roy, USA). .. Bacteria cells were stained with SYTO9 (1 μL, Invitrogen, S34854) for initial counting via epifluorescence microscopy with an Axio Observer Z1 inverted fluorescence microscope (Zeiss, Jean, Germany), yielding a concentration of 1.33×107 cells/mL. ..

    Article Title: Programmable Relaxation Dynamics in Bioinspired Coacervates for 3D Printing Cell Scaffolds.
    Article Snippet: Inspired by the tunable physical properties and biological roles of intracellular biomolecular condensates, this study developed chitosan and hyaluronic acid coacervates via liquid–liquid phase separation for 3D-printed cell scaffolds.. Multiscale analysis revealed that relaxation dynamics governed the structural organization and extrusion processability of coacervates.. Using timesalt-molecular weight-pH superposition principles, relaxation times were continuously and predictably tuned across seven orders of magnitude (10− 3 to 104 s) by modulating pH, ionic strength, and chain length, enabling programmable solid-gel-liquid transitions through a dynamic balance of electrostatic, hydrophobic, and hydration interactions.

    Fluorescence:

    Article Title: Interaction between FmRAV4 (a RAV transcription factor) and FmMYB1 (a MYB transcription factor) confers drought tolerance via ROS scavenging in Fraxinus mandshurica.
    Article Snippet: Drought stress severely restricts plant growth and development.. Although AP2/ERF family transcription factors (TFs) participate extensively in abiotic stress responses, they remain largely unexplored in Fraxinus mandshurica.. In this study, we identified and functionally characterized FmRAV4, a TF from the RAV subfamily.

    Article Title: Biomimetic mineralized hydrogels loaded with strontium-proanthocyanidins coordination particles for bone defect repair.
    Article Snippet: .. Fluorescence images were acquired using an inverted fluorescence microscope (Zeiss Axio Vert. ..

    Article Title: Modulating fibrillogenesis enhances the biofunctionality of dense collagen hydrogels
    Article Snippet: Slides were mounted using Vectamount AQ Aqueous Mounting Medium (Vector Laboratories) and cured overnight at room temperature. .. Sections were imaged using a Zeiss Axio-Observer.Z1 inverted fluorescence microscope for immunofluorescent detection or bright-field microscopy for H&E and chromogenic DAB stains. ..

    Article Title: Photoinduced hydrogel coatings hybridized with silver nanoparticles to prevent Nitratidesulfovibrio vulgaris adhesion and bio-corrosion of aluminum substrates
    Article Snippet: At the start of the experiment, the optical density at 600 nm was 0.014, measured using a Spectronic 401 (Spectronic Instruments, Milton Roy, USA). .. Bacteria cells were stained with SYTO9 (1 μL, Invitrogen, S34854) for initial counting via epifluorescence microscopy with an Axio Observer Z1 inverted fluorescence microscope (Zeiss, Jean, Germany), yielding a concentration of 1.33×107 cells/mL. ..

    Article Title: Programmable Relaxation Dynamics in Bioinspired Coacervates for 3D Printing Cell Scaffolds.
    Article Snippet: Inspired by the tunable physical properties and biological roles of intracellular biomolecular condensates, this study developed chitosan and hyaluronic acid coacervates via liquid–liquid phase separation for 3D-printed cell scaffolds.. Multiscale analysis revealed that relaxation dynamics governed the structural organization and extrusion processability of coacervates.. Using timesalt-molecular weight-pH superposition principles, relaxation times were continuously and predictably tuned across seven orders of magnitude (10− 3 to 104 s) by modulating pH, ionic strength, and chain length, enabling programmable solid-gel-liquid transitions through a dynamic balance of electrostatic, hydrophobic, and hydration interactions.

    Article Title: IscR-mediated morphological regulation confers virulence and stress resistance by reducing stress molecule uptake in Acinetobacter baumannii .
    Article Snippet: .. Fixed bacteria were immobilized on agarose pads and imaged using a Zeiss LSM 880 inverted confocal microscope or a Zeiss AxioObserver.Z1 inverted fluorescence microscope with a 63× oil immersion objective and a laser line of 488 nm for detection. ..

    Microscopy:

    Article Title: Interaction between FmRAV4 (a RAV transcription factor) and FmMYB1 (a MYB transcription factor) confers drought tolerance via ROS scavenging in Fraxinus mandshurica.
    Article Snippet: Drought stress severely restricts plant growth and development.. Although AP2/ERF family transcription factors (TFs) participate extensively in abiotic stress responses, they remain largely unexplored in Fraxinus mandshurica.. In this study, we identified and functionally characterized FmRAV4, a TF from the RAV subfamily.

    Article Title: Biomimetic mineralized hydrogels loaded with strontium-proanthocyanidins coordination particles for bone defect repair.
    Article Snippet: .. Fluorescence images were acquired using an inverted fluorescence microscope (Zeiss Axio Vert. ..

    Article Title: Computational identification and experimental validation of early glycinergic impairments in spinal muscular atrophy
    Article Snippet: Probes were purchased from ACD: Article in Press Scientific Reports https://doi.org/10.1038/s41598-026-74597-y Mm-Gcsh-O1-C1 (catalog #1240481-C1) and Mm-ChAT-C2 (catalog #408731-C2). .. The hybridized probe signal was acquired using a Zeiss Axio-observer Z1 inverted confocal microscope with a 25× oil objective. .. Spinal cord samples were homogenized in radioimmunoprecipitation assay buffer (RIPA lysis buffer, Merck Life Sciences, Darmstadt, Germany, ref. 20–188) supplemented with 1 mM phenylmethylsulfonyl fluoride (PMSF, Thermo Fisher Scientific, ref. 36978), 1 mM dithiothreitol (DTT, Thermo Fisher Scientific, ref. R0861), 2 mM sodium orthovanadate (Merck Life Sciences, ref. S6508) and 1 × complete Protease Inhibitor Cocktail (Merck Life Sciences, ref. 11697498001).

    Article Title: Modulating fibrillogenesis enhances the biofunctionality of dense collagen hydrogels
    Article Snippet: Slides were mounted using Vectamount AQ Aqueous Mounting Medium (Vector Laboratories) and cured overnight at room temperature. .. Sections were imaged using a Zeiss Axio-Observer.Z1 inverted fluorescence microscope for immunofluorescent detection or bright-field microscopy for H&E and chromogenic DAB stains. ..

    Article Title: Photoinduced hydrogel coatings hybridized with silver nanoparticles to prevent Nitratidesulfovibrio vulgaris adhesion and bio-corrosion of aluminum substrates
    Article Snippet: At the start of the experiment, the optical density at 600 nm was 0.014, measured using a Spectronic 401 (Spectronic Instruments, Milton Roy, USA). .. Bacteria cells were stained with SYTO9 (1 μL, Invitrogen, S34854) for initial counting via epifluorescence microscopy with an Axio Observer Z1 inverted fluorescence microscope (Zeiss, Jean, Germany), yielding a concentration of 1.33×107 cells/mL. ..

    Article Title: Programmable Relaxation Dynamics in Bioinspired Coacervates for 3D Printing Cell Scaffolds.
    Article Snippet: Inspired by the tunable physical properties and biological roles of intracellular biomolecular condensates, this study developed chitosan and hyaluronic acid coacervates via liquid–liquid phase separation for 3D-printed cell scaffolds.. Multiscale analysis revealed that relaxation dynamics governed the structural organization and extrusion processability of coacervates.. Using timesalt-molecular weight-pH superposition principles, relaxation times were continuously and predictably tuned across seven orders of magnitude (10− 3 to 104 s) by modulating pH, ionic strength, and chain length, enabling programmable solid-gel-liquid transitions through a dynamic balance of electrostatic, hydrophobic, and hydration interactions.

    Article Title: IscR-mediated morphological regulation confers virulence and stress resistance by reducing stress molecule uptake in Acinetobacter baumannii .
    Article Snippet: .. Fixed bacteria were immobilized on agarose pads and imaged using a Zeiss LSM 880 inverted confocal microscope or a Zeiss AxioObserver.Z1 inverted fluorescence microscope with a 63× oil immersion objective and a laser line of 488 nm for detection. ..

    Article Title: Early myelin-associated histological and cellular findings following ketogenic diet or ketone ester treatment in a cuprizone/rapamycin mouse model.
    Article Snippet: Background: Impaired restoration of CNS myelin contributes to progressive disability in multiple sclerosis (MS), yet current disease-modifying therapies do not directly target this biology.. Metabolic interventions such as the ketogenic diet (KD) are of interest, but sustained dietary adherence may be limited.. Methods: Male C57BL/6 mice underwent cuprizone/rapamycin demyelination for 6 weeks and, after an additional 7-day rapamycin washout with continued cuprizone exposure, received 9 days of standard diet (SD), ketogenic diet (KD), or intermittent ketone ester injections (iKE) during early recovery.

    Confocal:

    Article Title: Computational identification and experimental validation of early glycinergic impairments in spinal muscular atrophy
    Article Snippet: Probes were purchased from ACD: Article in Press Scientific Reports https://doi.org/10.1038/s41598-026-74597-y Mm-Gcsh-O1-C1 (catalog #1240481-C1) and Mm-ChAT-C2 (catalog #408731-C2). .. The hybridized probe signal was acquired using a Zeiss Axio-observer Z1 inverted confocal microscope with a 25× oil objective. .. Spinal cord samples were homogenized in radioimmunoprecipitation assay buffer (RIPA lysis buffer, Merck Life Sciences, Darmstadt, Germany, ref. 20–188) supplemented with 1 mM phenylmethylsulfonyl fluoride (PMSF, Thermo Fisher Scientific, ref. 36978), 1 mM dithiothreitol (DTT, Thermo Fisher Scientific, ref. R0861), 2 mM sodium orthovanadate (Merck Life Sciences, ref. S6508) and 1 × complete Protease Inhibitor Cocktail (Merck Life Sciences, ref. 11697498001).

    Article Title: IscR-mediated morphological regulation confers virulence and stress resistance by reducing stress molecule uptake in Acinetobacter baumannii .
    Article Snippet: .. Fixed bacteria were immobilized on agarose pads and imaged using a Zeiss LSM 880 inverted confocal microscope or a Zeiss AxioObserver.Z1 inverted fluorescence microscope with a 63× oil immersion objective and a laser line of 488 nm for detection. ..

    Article Title: Early myelin-associated histological and cellular findings following ketogenic diet or ketone ester treatment in a cuprizone/rapamycin mouse model.
    Article Snippet: Background: Impaired restoration of CNS myelin contributes to progressive disability in multiple sclerosis (MS), yet current disease-modifying therapies do not directly target this biology.. Metabolic interventions such as the ketogenic diet (KD) are of interest, but sustained dietary adherence may be limited.. Methods: Male C57BL/6 mice underwent cuprizone/rapamycin demyelination for 6 weeks and, after an additional 7-day rapamycin washout with continued cuprizone exposure, received 9 days of standard diet (SD), ketogenic diet (KD), or intermittent ketone ester injections (iKE) during early recovery.

    Bacteria:

    Article Title: Photoinduced hydrogel coatings hybridized with silver nanoparticles to prevent Nitratidesulfovibrio vulgaris adhesion and bio-corrosion of aluminum substrates
    Article Snippet: At the start of the experiment, the optical density at 600 nm was 0.014, measured using a Spectronic 401 (Spectronic Instruments, Milton Roy, USA). .. Bacteria cells were stained with SYTO9 (1 μL, Invitrogen, S34854) for initial counting via epifluorescence microscopy with an Axio Observer Z1 inverted fluorescence microscope (Zeiss, Jean, Germany), yielding a concentration of 1.33×107 cells/mL. ..

    Article Title: IscR-mediated morphological regulation confers virulence and stress resistance by reducing stress molecule uptake in Acinetobacter baumannii .
    Article Snippet: .. Fixed bacteria were immobilized on agarose pads and imaged using a Zeiss LSM 880 inverted confocal microscope or a Zeiss AxioObserver.Z1 inverted fluorescence microscope with a 63× oil immersion objective and a laser line of 488 nm for detection. ..

    Counting:

    Article Title: Photoinduced hydrogel coatings hybridized with silver nanoparticles to prevent Nitratidesulfovibrio vulgaris adhesion and bio-corrosion of aluminum substrates
    Article Snippet: At the start of the experiment, the optical density at 600 nm was 0.014, measured using a Spectronic 401 (Spectronic Instruments, Milton Roy, USA). .. Bacteria cells were stained with SYTO9 (1 μL, Invitrogen, S34854) for initial counting via epifluorescence microscopy with an Axio Observer Z1 inverted fluorescence microscope (Zeiss, Jean, Germany), yielding a concentration of 1.33×107 cells/mL. ..

    Epifluorescence Microscopy:

    Article Title: Photoinduced hydrogel coatings hybridized with silver nanoparticles to prevent Nitratidesulfovibrio vulgaris adhesion and bio-corrosion of aluminum substrates
    Article Snippet: At the start of the experiment, the optical density at 600 nm was 0.014, measured using a Spectronic 401 (Spectronic Instruments, Milton Roy, USA). .. Bacteria cells were stained with SYTO9 (1 μL, Invitrogen, S34854) for initial counting via epifluorescence microscopy with an Axio Observer Z1 inverted fluorescence microscope (Zeiss, Jean, Germany), yielding a concentration of 1.33×107 cells/mL. ..

    Concentration Assay:

    Article Title: Photoinduced hydrogel coatings hybridized with silver nanoparticles to prevent Nitratidesulfovibrio vulgaris adhesion and bio-corrosion of aluminum substrates
    Article Snippet: At the start of the experiment, the optical density at 600 nm was 0.014, measured using a Spectronic 401 (Spectronic Instruments, Milton Roy, USA). .. Bacteria cells were stained with SYTO9 (1 μL, Invitrogen, S34854) for initial counting via epifluorescence microscopy with an Axio Observer Z1 inverted fluorescence microscope (Zeiss, Jean, Germany), yielding a concentration of 1.33×107 cells/mL. ..

    Spinning Disk:

    Article Title: Early myelin-associated histological and cellular findings following ketogenic diet or ketone ester treatment in a cuprizone/rapamycin mouse model.
    Article Snippet: Background: Impaired restoration of CNS myelin contributes to progressive disability in multiple sclerosis (MS), yet current disease-modifying therapies do not directly target this biology.. Metabolic interventions such as the ketogenic diet (KD) are of interest, but sustained dietary adherence may be limited.. Methods: Male C57BL/6 mice underwent cuprizone/rapamycin demyelination for 6 weeks and, after an additional 7-day rapamycin washout with continued cuprizone exposure, received 9 days of standard diet (SD), ketogenic diet (KD), or intermittent ketone ester injections (iKE) during early recovery.

    Software:

    Article Title: Early myelin-associated histological and cellular findings following ketogenic diet or ketone ester treatment in a cuprizone/rapamycin mouse model.
    Article Snippet: Background: Impaired restoration of CNS myelin contributes to progressive disability in multiple sclerosis (MS), yet current disease-modifying therapies do not directly target this biology.. Metabolic interventions such as the ketogenic diet (KD) are of interest, but sustained dietary adherence may be limited.. Methods: Male C57BL/6 mice underwent cuprizone/rapamycin demyelination for 6 weeks and, after an additional 7-day rapamycin washout with continued cuprizone exposure, received 9 days of standard diet (SD), ketogenic diet (KD), or intermittent ketone ester injections (iKE) during early recovery.



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    Schematic depiction of the CWLD system for high-speed ALEX and FWM optoacoustic microscopy. ( a ) The system equips three CWLDs, each of which was controlled by a custom-designed electrical circuit driver and received ultrashort trigger signals provided by arbitrary function generators. AL, achromatic doublet lens; AMP, low noise amplifier; AWG, arbitrary waveform function generator; CWLD, continuous wave laser diode; DAQ, data acquisition card; GM, galvanometric mirror scanner; GMC, GM controller; IM, inverted <t>microscope;</t> L, plano-convex lens; M, dielectric mirror; OL, microscope objective lens; PH, pinhole; S, high-precision motorized stage; UT, ultrasound transducer. ( b ) Principle of using the CWLD for alternating laser excitation (ALEX) or Frequency-Wavelength Multiplexed (FWM) optoacoustic microscopy. (Driver inset adapted from ).
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    Image Search Results


    Journal: STAR Protocols

    Article Title: Protocol for large DNA transgenesis in mice using the Cas9+Bxb1 toolbox

    doi: 10.1016/j.xpro.2024.103022

    Figure Lengend Snippet:

    Article Snippet: Inverted microscope AxioObserver.D1 , Zeiss , .

    Techniques: Recombinant, Sequencing, Staining, Embryo Culture, Sterility, Mutagenesis, Plasmid Preparation, DNA Extraction, Software, Imaging, Nucleic Acid Electrophoresis, Electrophoresis, Electroporation, Inverted Microscopy, Transferring

    Schematic depiction of the CWLD system for high-speed ALEX and FWM optoacoustic microscopy. ( a ) The system equips three CWLDs, each of which was controlled by a custom-designed electrical circuit driver and received ultrashort trigger signals provided by arbitrary function generators. AL, achromatic doublet lens; AMP, low noise amplifier; AWG, arbitrary waveform function generator; CWLD, continuous wave laser diode; DAQ, data acquisition card; GM, galvanometric mirror scanner; GMC, GM controller; IM, inverted microscope; L, plano-convex lens; M, dielectric mirror; OL, microscope objective lens; PH, pinhole; S, high-precision motorized stage; UT, ultrasound transducer. ( b ) Principle of using the CWLD for alternating laser excitation (ALEX) or Frequency-Wavelength Multiplexed (FWM) optoacoustic microscopy. (Driver inset adapted from ).

    Journal: Scientific Reports

    Article Title: Overdriven laser diode optoacoustic microscopy

    doi: 10.1038/s41598-023-46855-w

    Figure Lengend Snippet: Schematic depiction of the CWLD system for high-speed ALEX and FWM optoacoustic microscopy. ( a ) The system equips three CWLDs, each of which was controlled by a custom-designed electrical circuit driver and received ultrashort trigger signals provided by arbitrary function generators. AL, achromatic doublet lens; AMP, low noise amplifier; AWG, arbitrary waveform function generator; CWLD, continuous wave laser diode; DAQ, data acquisition card; GM, galvanometric mirror scanner; GMC, GM controller; IM, inverted microscope; L, plano-convex lens; M, dielectric mirror; OL, microscope objective lens; PH, pinhole; S, high-precision motorized stage; UT, ultrasound transducer. ( b ) Principle of using the CWLD for alternating laser excitation (ALEX) or Frequency-Wavelength Multiplexed (FWM) optoacoustic microscopy. (Driver inset adapted from ).

    Article Snippet: The merging of the beams was achieved by longpass dichroic mirrors (DMLP490R and DMLP550R, Thorlabs) before encountering a set of high-precision galvanometric (galvo) mirrors (6215H Galvanometer Scanners, Cambridge Technologies) in combination with an inverted microscope stand (AxioObserver.D1, Zeiss) for laser-scanning.

    Techniques: Microscopy, Inverted Microscopy