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Promega amv-rt reaction buffer
Amv Rt Reaction Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/amv+rt+buffer/amv+buffer/pmc09508852-60-26-29
Average 90 stars, based on 1 article reviews
amv-rt reaction buffer - by Bioz Stars, 2026-10
90/100 stars

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Article Title: Gene expression profiling of calcium-channel antagonists in the heart of hypertensive and normotensive rats reveals class specific effects.
Article Snippet: Article history: Received 9 August 2016 Accepted 5 September 2016 Available online xxxx Calcium channel blockers (CCB) differ in their effects on the cardiovascular systemwith diltiazem being less negatively ionotrop as compared to verapamil.. Diltiazem is mainly used to treat supraventricular tachycardia, vasospastic angina and the Raynaud's syndrome.. Little is known about the molecular effects of benzothiazepins on cardiac gene expression.

Article Title: Human Thg1 displays tRNA-inducible GTPase activity
Article Snippet: Annealed primer was extended for 5 minute at room temperature, followed by 1-h reverse-transcription at 37°C with 20 μM dNTP and 0.4 U/μl AMV-reverse transcriptase in AMV-RT reaction buffer (Promega).

Article Title: Interplay between substrate recognition, 5’ end tRNA processing and methylation activity of human mitochondrial RNase P
Article Snippet: 2 μl of annealed primer/(mt)pre-tRNA mixture was added to a final volume of 5 μl reverse transcriptase reaction mixture containing 0.7 U of AMV-Reverse transcriptase (Promega), 1× AMV-RT reaction buffer (Promega) and 0.5 mM dNTP.

Article Title: Sarecycline inhibits protein translation in Cutibacterium acnes 70S ribosome using a two-site mechanism
Article Snippet: 1 μl of 32P end labeled primer (pr4pcrLuc, about 2.5 μM) was added to the reaction, incubated for 2 minutes at 37°C.To start cDNA synthesis 4 μl of RT-mix was added (0.5 μl of dNTP (10 mM each), 2 μl of AMV (5x) buffer, 1 μl of AMV RT (Promega, USA, cat. # M5101) and 0.5 μl of H2O) to the reaction and incubated for 30 minutes at 37°C.

Article Title: CPEB and miR-15/16 Co-Regulate Translation of Cyclin E1 mRNA during Xenopus Oocyte Maturation
Article Snippet: 8 μl of master mix containing 1μl 10mM dNTPs, 1μl of each 10 mM primer (3 per reaction) and 4 μl of AMV 5x buffer (Promega) was added to each sample.

Quantitative RT-PCR:

Article Title: Stage-Specific Global Alterations in the Transcriptomes of Lyme Disease Spirochetes During Tick Feeding and Following Mammalian Host-Adaptation
Article Snippet: .. Clustering was performed in GeneSpring GX12.5 with average linkage using the Pearson-centered correlation coefficient as the distance metric. qRT-PCR 4 μg of total tick RNA or 1 μg of in vitro or DMC cultivated B. burgdorferi RNA were converted to cDNA in a 20 μl reaction in AMV buffer (Promega, WI) containing 10 units of AMV reverse transcriptase (Promega), 500 ng of random hexamers (Promega), 250 μm of dNTPs and RNasin (5 U). ..

In Vitro:

Article Title: Stage-Specific Global Alterations in the Transcriptomes of Lyme Disease Spirochetes During Tick Feeding and Following Mammalian Host-Adaptation
Article Snippet: .. Clustering was performed in GeneSpring GX12.5 with average linkage using the Pearson-centered correlation coefficient as the distance metric. qRT-PCR 4 μg of total tick RNA or 1 μg of in vitro or DMC cultivated B. burgdorferi RNA were converted to cDNA in a 20 μl reaction in AMV buffer (Promega, WI) containing 10 units of AMV reverse transcriptase (Promega), 500 ng of random hexamers (Promega), 250 μm of dNTPs and RNasin (5 U). ..

Reverse Transcription:

Article Title: Stage-Specific Global Alterations in the Transcriptomes of Lyme Disease Spirochetes During Tick Feeding and Following Mammalian Host-Adaptation
Article Snippet: .. Clustering was performed in GeneSpring GX12.5 with average linkage using the Pearson-centered correlation coefficient as the distance metric. qRT-PCR 4 μg of total tick RNA or 1 μg of in vitro or DMC cultivated B. burgdorferi RNA were converted to cDNA in a 20 μl reaction in AMV buffer (Promega, WI) containing 10 units of AMV reverse transcriptase (Promega), 500 ng of random hexamers (Promega), 250 μm of dNTPs and RNasin (5 U). ..

Article Title: Evaluation of in vitro Assays to Assess the Modulation of Dendritic Cells Functions by Therapeutic Antibodies and Aggregates
Article Snippet: The reaction used 1 μg total RNA, a deoxynucleotide triphosphate mixture (containing 25 mM deoxy-adenosine triphosphate, deoxy-guanosine triphosphate, deoxy-cytidine triphosphate, and deoxy-thymidine triphosphate), and 50 mM oligo (dT) primers (MWG Biotech, Ebersberg, Germany). .. Reverse transcription was carried out in 1X AMV RT reaction buffer (Promega, Charbonnières-les-Bains, France) with RNase inhibitor (RNasine; Promega) at 40 U/μl, AMV RT (Promega) at 10 U/μl, and RNase-free water to a final volume of 10 μl. ..



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