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thermocycler gradient  (Eppendorf AG)


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    Structured Review

    Eppendorf AG thermocycler gradient
    Thermocycler Gradient, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 94/100, based on 89 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/adaptive/Adapter/pmc02500044-110-10-9
    Average 94 stars, based on 89 article reviews
    thermocycler gradient - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Binding Assay:

    Article Title: Method of characterizing a polynucleotide
    Article Snippet: The pelleted beads were washed with 140 μL of an adapter bead binding buffer, the beads were re-suspended in the adaptor bead binding buffer by two successive 180° rotations of the Eppendorf tube on the magnetic rack. .. The pelleted beads were washed with 140 μL of an adapter bead binding buffer, the beads were re-suspended in the adaptor bead binding buffer by two successive 180° rotations of the Eppendorf tube on the magnetic rack. ..

    Article Title: Method of characterizing a polynucleotide
    Article Snippet: The pelleted beads were washed with 140 L of an adapter bead binding buffer, the beads were re-suspended in the adaptor bead binding buffer by two successive 180° rotations of the Eppendorf tube on the magnetic rack. .. The pelleted beads were washed with 140 L of an adapter bead binding buffer, the beads were re-suspended in the adaptor bead binding buffer by two successive 180° rotations of the Eppendorf tube on the magnetic rack. .. The pelleted beads were again washed with 140 μL of the buffer, the beads were re-suspended in the buffer by two successive 180° rotations of the Eppendorf tube on the magnetic rack.

    other:

    Article Title: A beginners guide to Sf9 and Sf21 insect cell line culture and troubleshooting
    Article Snippet: Ampicillin (Bioshop; cat. no. AMP201.25) Chloramphenicol (Bioshop; cat. no. CLR201.25) Gentamycin (Bioshop; cat. no. GTA202.5) Kanamycin (Bioshop; cat. no. KAN201.25) Tetracycline (Bioshop; cat. no. TET701.25) Bactotryptone (Bioshop; cat. no. TRP402.500) Yeast Extract (Bioshop; cat. no. YEX401.500) Glycerol (Bioshop; cat. no. GLY001.4) Potassium phosphate monobasic (Bioshop; cat. no. PPM302.1) Potassium phosphate dibasic (Bioshop; cat. no. PPD303.1) IPTG (BioShop; cat. no. IPT001.25) XGAL (20 mg/mL, BioShop; cat. no XGA002.510) LB (Miller) broth (Bioshop; cat. no. LBL407.1) LB (Miller) agar (Bioshop; cat. no. LBA408.1) Tris (Bioshop; TRS001.5) EDTA (0.5 M, Invitrogen; cat no. AM9260G) RNase A (Merck; cat. no. 10109169001) NaOH (Stanlab; 011-002-00-6) SDS (Bioshop; cat. no. SDS001.1) Potassium acetate (Merck; cat. no. 60035-50G) HCl 35–38% (Stanlab; cat. no 017-002-01-X) Isopropanol (Stanlab; cat. no 603-117-00-0) Ethanol 99.9% (Stanlab; cat. no. 603-002-00-5)

    Article Title: Method of characterizing a polynucleotide
    Article Snippet: 222 μl of Agencourt AMPure beads (Beckman Coulter) were added and the sample incubated for 5 mins at room temperature on a rotator.

    Adapter Ligation:

    Article Title: Long-read sequencing and structural variant characterization in 1,019 samples from the 1000 Genomes Project
    Article Snippet: .. For adapter ligation and clean-up, 60 μl DNA from the previous step was combined with 25 μl Ligation Buffer LNB, 10 μl NEBNext Quick T4 DNA Ligase and 5 μl Adapter Mix AMX in a 1.5 ml Eppendorf DNA LoBind tube. ..

    Ligation:

    Article Title: Long-read sequencing and structural variant characterization in 1,019 samples from the 1000 Genomes Project
    Article Snippet: .. For adapter ligation and clean-up, 60 μl DNA from the previous step was combined with 25 μl Ligation Buffer LNB, 10 μl NEBNext Quick T4 DNA Ligase and 5 μl Adapter Mix AMX in a 1.5 ml Eppendorf DNA LoBind tube. ..

    Article Title: Structural variation in 1,019 diverse humans based on long-read sequencing
    Article Snippet: .. For adaptor ligation and clean-up, 60 μl of DNA from the previous step was combined with 25 μl of Ligation Buffer, 10 μl of NEBNext Quick T4 DNA Ligase and 5 μl of Adapter Mix in a 1.5-ml Eppendorf DNA LoBind tube. ..

    Polymerase Chain Reaction:

    Article Title: Method and apparatus for processing tissue samples
    Article Snippet: .. In another embodiment processing comprises one or more automatically performed processes selected from: (I) lysing cells; (II) capturing cells; (Ill) isolating nucleic acid; (IV) isolating protein; (V) converting RNA into cDNA; (VI) preparing one or more libraries of adapter tagged nucleic acids; (VII) performing PCR or other DNA amplification methods; (VIII) isolating individual cells or individual nuclei in nanodrops or nanoboluses or nanowells or in media such as agarose that will limit diffusion of molecules of interest between cells or nuclei; and (IX) outputting released cells and/or nuclei into output vessels such as 8 well strip tubes, microtiter plates, Eppendorf tubes, a chamber in the cartridge, or other vessels capable of receiving cell suspensions. ..

    DNA Amplification:

    Article Title: Method and apparatus for processing tissue samples
    Article Snippet: .. In another embodiment processing comprises one or more automatically performed processes selected from: (I) lysing cells; (II) capturing cells; (Ill) isolating nucleic acid; (IV) isolating protein; (V) converting RNA into cDNA; (VI) preparing one or more libraries of adapter tagged nucleic acids; (VII) performing PCR or other DNA amplification methods; (VIII) isolating individual cells or individual nuclei in nanodrops or nanoboluses or nanowells or in media such as agarose that will limit diffusion of molecules of interest between cells or nuclei; and (IX) outputting released cells and/or nuclei into output vessels such as 8 well strip tubes, microtiter plates, Eppendorf tubes, a chamber in the cartridge, or other vessels capable of receiving cell suspensions. ..

    Diffusion-based Assay:

    Article Title: Method and apparatus for processing tissue samples
    Article Snippet: .. In another embodiment processing comprises one or more automatically performed processes selected from: (I) lysing cells; (II) capturing cells; (Ill) isolating nucleic acid; (IV) isolating protein; (V) converting RNA into cDNA; (VI) preparing one or more libraries of adapter tagged nucleic acids; (VII) performing PCR or other DNA amplification methods; (VIII) isolating individual cells or individual nuclei in nanodrops or nanoboluses or nanowells or in media such as agarose that will limit diffusion of molecules of interest between cells or nuclei; and (IX) outputting released cells and/or nuclei into output vessels such as 8 well strip tubes, microtiter plates, Eppendorf tubes, a chamber in the cartridge, or other vessels capable of receiving cell suspensions. ..

    Stripping Membranes:

    Article Title: Method and apparatus for processing tissue samples
    Article Snippet: .. In another embodiment processing comprises one or more automatically performed processes selected from: (I) lysing cells; (II) capturing cells; (Ill) isolating nucleic acid; (IV) isolating protein; (V) converting RNA into cDNA; (VI) preparing one or more libraries of adapter tagged nucleic acids; (VII) performing PCR or other DNA amplification methods; (VIII) isolating individual cells or individual nuclei in nanodrops or nanoboluses or nanowells or in media such as agarose that will limit diffusion of molecules of interest between cells or nuclei; and (IX) outputting released cells and/or nuclei into output vessels such as 8 well strip tubes, microtiter plates, Eppendorf tubes, a chamber in the cartridge, or other vessels capable of receiving cell suspensions. ..



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    FPD response as a function of PIFR for the 26 runs. (A) The runs in red are counted in the desired FPD range (600 ± 200 μg) and (B) the runs in red are counted in the constant FPD region (750 ± 200 μg) for the low resistance RS01 DPI. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Journal: International Journal of Pharmaceutics: X

    Article Title: Determination of the optimal resistance in RS01 for robust aerosol performance of inhaled controlled-release budesonide powder for chronic obstructive inflammatory respiratory diseases patients

    doi: 10.1016/j.ijpx.2026.100581

    Figure Lengend Snippet: FPD response as a function of PIFR for the 26 runs. (A) The runs in red are counted in the desired FPD range (600 ± 200 μg) and (B) the runs in red are counted in the constant FPD region (750 ± 200 μg) for the low resistance RS01 DPI. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Article Snippet: An impaction test is performed using three size 3 HPMC capsules filled with 20 ± 0.5 mg of the blend, inserted into the RS01 inhaler connected onto the AIT using an RS01 adapter (Copley Scientific).

    Techniques:

    FPD response as a function of PIFR for 23 runs. (A) The runs in red are counted in the desired robust FPD region (600 ± 200 μg) and (B) The runs in red are counted in the constant FPD region (650 ± 200 μg) for the low-medium resistance RS01 DPI. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Journal: International Journal of Pharmaceutics: X

    Article Title: Determination of the optimal resistance in RS01 for robust aerosol performance of inhaled controlled-release budesonide powder for chronic obstructive inflammatory respiratory diseases patients

    doi: 10.1016/j.ijpx.2026.100581

    Figure Lengend Snippet: FPD response as a function of PIFR for 23 runs. (A) The runs in red are counted in the desired robust FPD region (600 ± 200 μg) and (B) The runs in red are counted in the constant FPD region (650 ± 200 μg) for the low-medium resistance RS01 DPI. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Article Snippet: An impaction test is performed using three size 3 HPMC capsules filled with 20 ± 0.5 mg of the blend, inserted into the RS01 inhaler connected onto the AIT using an RS01 adapter (Copley Scientific).

    Techniques: