thermocycler gradient (Eppendorf AG)

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Eppendorf AG thermocycler gradient
Thermocycler Gradient, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 94/100, based on 89 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/adaptative/Adapter/pmc02500044-110-10-9
Average 94 stars, based on 89 article reviews

thermocycler gradient - by Bioz Stars, 2026-09

94/100 stars

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Binding Assay:

Article Title: Method of characterizing a polynucleotide
Article Snippet: The pelleted beads were washed with 140 μL of an adapter bead binding buffer, the beads were re-suspended in the adaptor bead binding buffer by two successive 180° rotations of the Eppendorf tube on the magnetic rack. .. The pelleted beads were washed with 140 μL of an adapter bead binding buffer, the beads were re-suspended in the adaptor bead binding buffer by two successive 180° rotations of the Eppendorf tube on the magnetic rack. ..

Article Title: Method of characterizing a polynucleotide
Article Snippet: The pelleted beads were washed with 140 L of an adapter bead binding buffer, the beads were re-suspended in the adaptor bead binding buffer by two successive 180° rotations of the Eppendorf tube on the magnetic rack. .. The pelleted beads were washed with 140 L of an adapter bead binding buffer, the beads were re-suspended in the adaptor bead binding buffer by two successive 180° rotations of the Eppendorf tube on the magnetic rack. .. The pelleted beads were again washed with 140 μL of the buffer, the beads were re-suspended in the buffer by two successive 180° rotations of the Eppendorf tube on the magnetic rack.

Cell Counting:

Article Title: A beginners guide to Sf9 and Sf21 insect cell line culture and troubleshooting
Article Snippet: Ampicillin (Bioshop; cat. no. AMP201.25) Chloramphenicol (Bioshop; cat. no. CLR201.25) Gentamycin (Bioshop; cat. no. GTA202.5) Kanamycin (Bioshop; cat. no. KAN201.25) Tetracycline (Bioshop; cat. no. TET701.25) Bactotryptone (Bioshop; cat. no. TRP402.500) Yeast Extract (Bioshop; cat. no. YEX401.500) Glycerol (Bioshop; cat. no. GLY001.4) Potassium phosphate monobasic (Bioshop; cat. no. PPM302.1) Potassium phosphate dibasic (Bioshop; cat. no. PPD303.1) IPTG (BioShop; cat. no. IPT001.25) XGAL (20 mg/mL, BioShop; cat. no XGA002.510) LB (Miller) broth (Bioshop; cat. no. LBL407.1) LB (Miller) agar (Bioshop; cat. no. LBA408.1) Tris (Bioshop; TRS001.5) EDTA (0.5 M, Invitrogen; cat no. AM9260G) RNase A (Merck; cat. no. 10109169001) NaOH (Stanlab; 011-002-00-6) SDS (Bioshop; cat. no. SDS001.1) Potassium acetate (Merck; cat. no. 60035-50G) HCl 35–38% (Stanlab; cat. no 017-002-01-X) Isopropanol (Stanlab; cat. no 603-117-00-0) Ethanol 99.9% (Stanlab; cat. no. 603-002-00-5) .. Countess II FL Automated Cell Counter Assay Platform AMQAF1000 (New Life Technologies) CountessTM Cell Counting Chamber Slides (Thermo Fisher; cat. no. C10312 ) Neubauer chamber (cat. no. LLG-9161086) Hand Tally Click Counter (cat. no. HS6594) Plastic Cell Culture Flask 25 cm 2 50 mL (Greiner Bio-One; cat. no. 690175), Glass Erlenmeyer Flasks, narrow mouth style: 25 mL (Duran; cat. no. 320/A10) Glass Erlenmeyer Flasks, narrow mouth style 100 mL (Duran; cat. no. G-2052) Glass Erlenmeyer Flasks, narrow mouth style 250 mL (Duran; cat. no. G-2053) Glass Erlenmeyer Flasks, narrow mouth style 500 mL (Duran; cat. no. G-2055) Glass Erlenmeyer Flasks, narrow mouth style 2000 mL (Duran; cat. no. G-2057) Metal lids (Schuett-Biotec; cat. no. B-2964) Falcon tubes 15 m L (Sarstedt; cat. no. 62.554.502) Falcon tubes 50 mL (Sarstedt; cat. no. 62.548.004) Cryo vials (Sarstedt; cat. no. J-1958) Serological pipettes (Greiner Bio-one; cat. no. 1 mL 612–1011) Serological pipettes (Greiner Bio-one; cat. no. 2 mL 612–1012) Serological pipettes (Greiner Bio-one; cat. no. 5 mL 612–1013) Serological pipettes (Greiner Bio-one; cat. no. 10 mL 612–1015) Serological pipettes (Greiner Bio-one; cat. no. 25 mL 612–1017) Serological pipettes (Greiner Bio-one; cat. no. 50 mL 612–1018) Pipetboy SWIFT PRO (HTL; cat. no. 390) Freezing container Mr. Frosty (Nalgene; cat. no. L-0388) Centrifuges with rotors/adapters for 1 5 mL and 50 mL Falcon tubes and Eppendorf tubes Incubator without CO 2 Incubator without CO 2 with shaking platform Laminar flow hood Fluorescent Microscope (or fluorescent plate reader, imager) Neutral Red (Sigma Aldrich; cat. no. N2889-100ML) Pipettes 1000 μL, 200 μL, 20 μL, 10 μL HTL Vortex Burner Syringe filters 0.22 μm (Merck; cat. no. SLGP033RS) Syringes (5 mL cat. no. 309 050; 10 mL cat. no. 309 110; 20 mL cat. no.300 296 BD) Needles (cat.no. ..

Cell Culture:

Microscopy:

Adapter Ligation:

Article Title: Long-read sequencing and structural variant characterization in 1,019 samples from the 1000 Genomes Project
Article Snippet: .. For adapter ligation and clean-up, 60 μl DNA from the previous step was combined with 25 μl Ligation Buffer LNB, 10 μl NEBNext Quick T4 DNA Ligase and 5 μl Adapter Mix AMX in a 1.5 ml Eppendorf DNA LoBind tube. ..

Ligation:

Article Title: Structural variation in 1,019 diverse humans based on long-read sequencing
Article Snippet: .. For adaptor ligation and clean-up, 60 μl of DNA from the previous step was combined with 25 μl of Ligation Buffer, 10 μl of NEBNext Quick T4 DNA Ligase and 5 μl of Adapter Mix in a 1.5-ml Eppendorf DNA LoBind tube. ..

Polymerase Chain Reaction:

Article Title: Method and apparatus for processing tissue samples
Article Snippet: .. In another embodiment processing comprises one or more automatically performed processes selected from: (I) lysing cells; (II) capturing cells; (Ill) isolating nucleic acid; (IV) isolating protein; (V) converting RNA into cDNA; (VI) preparing one or more libraries of adapter tagged nucleic acids; (VII) performing PCR or other DNA amplification methods; (VIII) isolating individual cells or individual nuclei in nanodrops or nanoboluses or nanowells or in media such as agarose that will limit diffusion of molecules of interest between cells or nuclei; and (IX) outputting released cells and/or nuclei into output vessels such as 8 well strip tubes, microtiter plates, Eppendorf tubes, a chamber in the cartridge, or other vessels capable of receiving cell suspensions. ..

DNA Amplification:

Diffusion-based Assay:

Stripping Membranes:

other:

Article Title: Method of characterizing a polynucleotide
Article Snippet: 222 μl of Agencourt AMPure beads (Beckman Coulter) were added and the sample incubated for 5 mins at room temperature on a rotator.

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