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direct zol rna miniprep kit  (Zymo Research)


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    Structured Review

    Zymo Research direct zol rna miniprep kit

    Direct Zol Rna Miniprep Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 10628 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/actin+polymerization+depolymerization+kit/Direct-zol+RNA+Miniprep/pmc11648168-143-0-5
    Average 99 stars, based on 10628 article reviews
    direct zol rna miniprep kit - by Bioz Stars, 2026-11
    99/100 stars

    Images

    1) Product Images from "Nanoparticle-based itaconate treatment recapitulates low-cholesterol/low-fat diet-induced atherosclerotic plaque resolution"

    Article Title: Nanoparticle-based itaconate treatment recapitulates low-cholesterol/low-fat diet-induced atherosclerotic plaque resolution

    Journal: Cell reports

    doi: 10.1016/j.celrep.2024.114911


    Figure Legend Snippet:

    Techniques Used: Purification, Plasmid Preparation, Produced, Recombinant, Concentration Assay, Saline, Labeling, Membrane, Enzyme-linked Immunosorbent Assay, Quantitation Assay, Phospholipid Assay, BIA-KA, RNAscope, HD Assay, Polymer, Sequencing, Expressing, Software, Microscopy

    Related Articles

    Purification:

    Article Title: The role of the NLRP3 inflammasome in a CLN2 mouse model of neuroinflammation
    Article Snippet: For MCC950 experiments, brain slice cultures were exposed to 10 or 50 nM MCC950 (APExBIO; B7946), or water (VEH) directly added to slice culture media for 6 hours (6h). .. RNA was purified from Trizol-homogenized brain or slice cultures (Invitrogen; 5596018) using the Direct-zol RNA Miniprep Kit (Zymo Research; R2052) and reverse-transcribed with the High-Capacity Reverse Transcription Kit (Applied Biosystems; 4368814). .. RT-qPCRs were performed using the Sso Advanced TM Universal SYBR Green system (Bio-Rad Laboratories; 1725121) in the CFX Duet real time instrument (Bio-Rad Laboratories).

    Article Title: Aminoacylated tRNA tails trigger auxiliary bacterial immunity
    Article Snippet: For small RNA sequencing, 25 μl of DNase-treated RNA was treated with T4 polynucleotide kinase (PNK; New England Biolabs) in the presence of ATP for 30 min at 37 °C. .. Following PNK treatment, RNA was extracted with TRI Reagent and purified using the Direct-zol RNA MiniPrep kit (Zymo Research) to remove residual enzyme before subsequent adapter ligation. ..

    Article Title: Neurobiological alterations and autism-like phenotype in mice following regulatory-limit exposure to fungicides in drinking water
    Article Snippet: .. RNAs were extracted and purified using the Direct-Zol RNA MiniPrep kit (Zymo research, Irvine, USA). .. cDNA was synthetized using the ProtoScript II Reverse Transcriptase kit (New England BioLabs, Evry-Courcouronnes, France).

    Article Title: Subchondral bone remodeling in knee osteoarthritis is coupled to lipid mobilization from bone marrow adipocytes
    Article Snippet: .. RNA was extracted and purified using the Direct-zol RNA Miniprep Kit (Zymo Research, #R2052) according to the manufacturer’s instructions. .. RNA concentration and purity were assessed using a NanoDrop spectrophotometer (Thermo Fisher Scientific).

    Reverse Transcription:

    Article Title: The role of the NLRP3 inflammasome in a CLN2 mouse model of neuroinflammation
    Article Snippet: For MCC950 experiments, brain slice cultures were exposed to 10 or 50 nM MCC950 (APExBIO; B7946), or water (VEH) directly added to slice culture media for 6 hours (6h). .. RNA was purified from Trizol-homogenized brain or slice cultures (Invitrogen; 5596018) using the Direct-zol RNA Miniprep Kit (Zymo Research; R2052) and reverse-transcribed with the High-Capacity Reverse Transcription Kit (Applied Biosystems; 4368814). .. RT-qPCRs were performed using the Sso Advanced TM Universal SYBR Green system (Bio-Rad Laboratories; 1725121) in the CFX Duet real time instrument (Bio-Rad Laboratories).

    Adapter Ligation:

    Article Title: Aminoacylated tRNA tails trigger auxiliary bacterial immunity
    Article Snippet: For small RNA sequencing, 25 μl of DNase-treated RNA was treated with T4 polynucleotide kinase (PNK; New England Biolabs) in the presence of ATP for 30 min at 37 °C. .. Following PNK treatment, RNA was extracted with TRI Reagent and purified using the Direct-zol RNA MiniPrep kit (Zymo Research) to remove residual enzyme before subsequent adapter ligation. ..

    Isolation:

    Article Title: Synaptic microRNA dysregulation orchestrates aberrant glutamatergic transmission in a human neuronal model of 22q11.2 deletion syndrome
    Article Snippet: .. Neurons were homogenized in TRIzol Reagent (Invitrogen, 15596018), and RNA was isolated according to the manufacturer’s instructions using the Direct-zol RNA Miniprep Kit (Zymo, R2052). .. miRNA cDNA was synthesized using the TaqMan Advanced miRNA cDNA Synthesis Kit (Cat. No. A28007) according to the manufacturer’s instructions.

    Article Title: A zebrafish ribosomopathy links Ddx49-dependent ribosome biogenesis to differential failure of population expansion among pigment cell lineages
    Article Snippet: For 7.5 SSL, fish were euthanized, heads removed and trunks rinsed with 1× PBS with four trunks per replicate transferred into 1 ml ice-cold TRIzol (Invitrogen) in a 2 ml Eppendorf LoBind tube with one 1/4” ceramic sphere (MP Biomedicals) and 50–100 ul of Lysing Matrix A (MP Biomedicals), then homogenized using a Tissuelyser II (Qiagen) at maximum speed for 30 s. Homogenized mixtures were either processed immediately for RNA isolation or stored at -80°C for long-term storage. .. Subsequent total RNA isolation and on-column DNase treatment were performed using Direct-zol RNA Miniprep Kits (Zymo Research) per manufacturer instructions. .. RNA concentration and purity were assessed using a DS-11+ spectrophotometer (DeNovix).



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    Image Search Results


    List of differentially expressed salt stress responsive ggenes selected by the MapMan program.

    Journal: PeerJ

    Article Title: Identification and transcriptomic profiling of salinity stress response genes in colored wheat mutant

    doi: 10.7717/peerj.17043

    Figure Lengend Snippet: List of differentially expressed salt stress responsive ggenes selected by the MapMan program.

    Article Snippet: TraesCS5D02G492300 , Actin depolymerization factor-like protein , 147 , 6.3E−105 , 87.63 , 0.55 , 0.09 , 0.38 , −3 . 25 , 2.08E−11 , 5.07E−10 , Cytoskeleton organization.

    Techniques: