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Biomol GmbH substrate ac-devd-afc
Substrate Ac Devd Afc, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ac-devd-afc/ac+devd+afc+substrate/pm27924873-201-1-18
Average 90 stars, based on 1 article reviews
substrate ac-devd-afc - by Bioz Stars, 2026-11
90/100 stars

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Related Articles

Activation Assay:

Article Title: Aβ(1-42) aggregates into non-toxic amyloid assemblies in the presence of the natural polyphenol oleuropein aglycon.
Article Snippet: Amyloid aggregation starts with the initial misfolding of peptide/protein precursors, with subsequent structural rearrangement into oligomers and protofibrils; the latter eventually organize into fibrils with shared basic structural features, found deposited in amyloid diseases.. Mounting evidence indicates early oligomers as the most toxic amyloid species; accordingly, the search of inhibitors of their growth is considered a promising target to prevent amyloid toxicity.. We recently showed that oleuropein aglycon, a polyphenol abundant in the extra virgin olive oil, interferes with the aggregation of amylin (involved in type-2 diabetes), eliminating its cytotoxicity.

Article Title: Variations of thioredoxin system contributes to increased susceptibility to apoptosis in cardiomyocytes of type 2 diabetic rats.
Article Snippet: Determination of caspase-3 protease activity and myocardial apoptotic death The substrates Ac-DEVD-AFC, Ac-IETC-AFC, and Ac-LEHD-AFC were used, respectively, to determine the caspase (-3, -8, and -9) protease activity according to the manufacturer’s instructions (Biomol, Houston, USA) [16].

Article Title: Cardiac Specific Overexpression of Mitochondrial Omi/HtrA2 Induces Myocardial Apoptosis and Cardiac Dysfunction.
Article Snippet: The substrates Ac-DEVD-AFC, and Ac-LEHD-AFC were used to determine caspase-3, and caspase-9 activity respectively, following the manufacturer’s guidelines (BIOMOL, USA).

Article Title: Mesenchymal Stem Cell Survival in the Infarcted Heart Is Enhanced by Lentivirus Vector-Mediated Heat Shock Protein 27 Expression
Article Snippet: Caspase activation was also examined by a quantitative caspase-3-like enzyme activity assay using Ac-DEVD-AFC substrate (Biomol, Enzo Life Sciences) and staurosporine (STS; 500 n M for 12 hr) as a positive control.

Article Title: Modulation of neutrophil apoptosis by murine pulmonary microvascular endothelial cell inducible nitric oxide synthase.
Article Snippet: Neutrophils contribute significantly to ALI (acute lung injury) through adhesion to pulmonary microvascular endothelial cells (PMEC), trans-PMEC migration and alveolar infiltration.. Trans-PMEC migration delays expression of neutrophil apoptosis, which promotes intra-alveolar neutrophil survival and neutrophil mediated ALI.. We assessed the role of neutrophil vs PMEC inducible nitric oxide (NO) synthase (iNOS) in modulating neutrophil apoptosis.

Article Title: Lentiviral vector mediated modification of mesenchymal stem cells & enhanced survival in an in vitro model of ischaemia
Article Snippet: Caspase-3 activation was also examined using Ac-DEVD-AFC substrate (Biomol, Enzo Life Sciences, PA, USA).

Article Title: miR-145 induces caspase-dependent and -independent cell death in urothelial cancer cell lines with targeting of an expression signature present in Ta bladder tumors.
Article Snippet: The kinetic cleavage of the substrate Ac-DEVD-AFC (Biomol, Plymouth Meeting, PA, USA) as measured by the liberation of AFC (excitation, 400 nm; emission, 489 nm) was measured in cell lysates using a Synergy HT multi-mode micro plate reader, (Biotek Industries Inc., Winooski, VT, USA).

Enzyme Activity Assay:

Article Title: Aβ(1-42) aggregates into non-toxic amyloid assemblies in the presence of the natural polyphenol oleuropein aglycon.
Article Snippet: Amyloid aggregation starts with the initial misfolding of peptide/protein precursors, with subsequent structural rearrangement into oligomers and protofibrils; the latter eventually organize into fibrils with shared basic structural features, found deposited in amyloid diseases.. Mounting evidence indicates early oligomers as the most toxic amyloid species; accordingly, the search of inhibitors of their growth is considered a promising target to prevent amyloid toxicity.. We recently showed that oleuropein aglycon, a polyphenol abundant in the extra virgin olive oil, interferes with the aggregation of amylin (involved in type-2 diabetes), eliminating its cytotoxicity.

Article Title: Variations of thioredoxin system contributes to increased susceptibility to apoptosis in cardiomyocytes of type 2 diabetic rats.
Article Snippet: Determination of caspase-3 protease activity and myocardial apoptotic death The substrates Ac-DEVD-AFC, Ac-IETC-AFC, and Ac-LEHD-AFC were used, respectively, to determine the caspase (-3, -8, and -9) protease activity according to the manufacturer’s instructions (Biomol, Houston, USA) [16].

Article Title: Cardiac Specific Overexpression of Mitochondrial Omi/HtrA2 Induces Myocardial Apoptosis and Cardiac Dysfunction.
Article Snippet: The substrates Ac-DEVD-AFC, and Ac-LEHD-AFC were used to determine caspase-3, and caspase-9 activity respectively, following the manufacturer’s guidelines (BIOMOL, USA).

Article Title: Mesenchymal Stem Cell Survival in the Infarcted Heart Is Enhanced by Lentivirus Vector-Mediated Heat Shock Protein 27 Expression
Article Snippet: Caspase activation was also examined by a quantitative caspase-3-like enzyme activity assay using Ac-DEVD-AFC substrate (Biomol, Enzo Life Sciences) and staurosporine (STS; 500 n M for 12 hr) as a positive control.

Article Title: Modulation of neutrophil apoptosis by murine pulmonary microvascular endothelial cell inducible nitric oxide synthase.
Article Snippet: Neutrophils contribute significantly to ALI (acute lung injury) through adhesion to pulmonary microvascular endothelial cells (PMEC), trans-PMEC migration and alveolar infiltration.. Trans-PMEC migration delays expression of neutrophil apoptosis, which promotes intra-alveolar neutrophil survival and neutrophil mediated ALI.. We assessed the role of neutrophil vs PMEC inducible nitric oxide (NO) synthase (iNOS) in modulating neutrophil apoptosis.

Article Title: Lentiviral vector mediated modification of mesenchymal stem cells & enhanced survival in an in vitro model of ischaemia
Article Snippet: Caspase-3 activation was also examined using Ac-DEVD-AFC substrate (Biomol, Enzo Life Sciences, PA, USA).

Article Title: miR-145 induces caspase-dependent and -independent cell death in urothelial cancer cell lines with targeting of an expression signature present in Ta bladder tumors.
Article Snippet: The kinetic cleavage of the substrate Ac-DEVD-AFC (Biomol, Plymouth Meeting, PA, USA) as measured by the liberation of AFC (excitation, 400 nm; emission, 489 nm) was measured in cell lysates using a Synergy HT multi-mode micro plate reader, (Biotek Industries Inc., Winooski, VT, USA).

Positive Control:

Article Title: Aβ(1-42) aggregates into non-toxic amyloid assemblies in the presence of the natural polyphenol oleuropein aglycon.
Article Snippet: Amyloid aggregation starts with the initial misfolding of peptide/protein precursors, with subsequent structural rearrangement into oligomers and protofibrils; the latter eventually organize into fibrils with shared basic structural features, found deposited in amyloid diseases.. Mounting evidence indicates early oligomers as the most toxic amyloid species; accordingly, the search of inhibitors of their growth is considered a promising target to prevent amyloid toxicity.. We recently showed that oleuropein aglycon, a polyphenol abundant in the extra virgin olive oil, interferes with the aggregation of amylin (involved in type-2 diabetes), eliminating its cytotoxicity.

Article Title: Variations of thioredoxin system contributes to increased susceptibility to apoptosis in cardiomyocytes of type 2 diabetic rats.
Article Snippet: Determination of caspase-3 protease activity and myocardial apoptotic death The substrates Ac-DEVD-AFC, Ac-IETC-AFC, and Ac-LEHD-AFC were used, respectively, to determine the caspase (-3, -8, and -9) protease activity according to the manufacturer’s instructions (Biomol, Houston, USA) [16].

Article Title: Cardiac Specific Overexpression of Mitochondrial Omi/HtrA2 Induces Myocardial Apoptosis and Cardiac Dysfunction.
Article Snippet: The substrates Ac-DEVD-AFC, and Ac-LEHD-AFC were used to determine caspase-3, and caspase-9 activity respectively, following the manufacturer’s guidelines (BIOMOL, USA).

Article Title: Mesenchymal Stem Cell Survival in the Infarcted Heart Is Enhanced by Lentivirus Vector-Mediated Heat Shock Protein 27 Expression
Article Snippet: Caspase activation was also examined by a quantitative caspase-3-like enzyme activity assay using Ac-DEVD-AFC substrate (Biomol, Enzo Life Sciences) and staurosporine (STS; 500 n M for 12 hr) as a positive control.

Article Title: Modulation of neutrophil apoptosis by murine pulmonary microvascular endothelial cell inducible nitric oxide synthase.
Article Snippet: Neutrophils contribute significantly to ALI (acute lung injury) through adhesion to pulmonary microvascular endothelial cells (PMEC), trans-PMEC migration and alveolar infiltration.. Trans-PMEC migration delays expression of neutrophil apoptosis, which promotes intra-alveolar neutrophil survival and neutrophil mediated ALI.. We assessed the role of neutrophil vs PMEC inducible nitric oxide (NO) synthase (iNOS) in modulating neutrophil apoptosis.

Article Title: Lentiviral vector mediated modification of mesenchymal stem cells & enhanced survival in an in vitro model of ischaemia
Article Snippet: Caspase-3 activation was also examined using Ac-DEVD-AFC substrate (Biomol, Enzo Life Sciences, PA, USA).

Article Title: miR-145 induces caspase-dependent and -independent cell death in urothelial cancer cell lines with targeting of an expression signature present in Ta bladder tumors.
Article Snippet: The kinetic cleavage of the substrate Ac-DEVD-AFC (Biomol, Plymouth Meeting, PA, USA) as measured by the liberation of AFC (excitation, 400 nm; emission, 489 nm) was measured in cell lysates using a Synergy HT multi-mode micro plate reader, (Biotek Industries Inc., Winooski, VT, USA).

Sonication:

Article Title: Aβ(1-42) aggregates into non-toxic amyloid assemblies in the presence of the natural polyphenol oleuropein aglycon.
Article Snippet: Amyloid aggregation starts with the initial misfolding of peptide/protein precursors, with subsequent structural rearrangement into oligomers and protofibrils; the latter eventually organize into fibrils with shared basic structural features, found deposited in amyloid diseases.. Mounting evidence indicates early oligomers as the most toxic amyloid species; accordingly, the search of inhibitors of their growth is considered a promising target to prevent amyloid toxicity.. We recently showed that oleuropein aglycon, a polyphenol abundant in the extra virgin olive oil, interferes with the aggregation of amylin (involved in type-2 diabetes), eliminating its cytotoxicity.

Article Title: Variations of thioredoxin system contributes to increased susceptibility to apoptosis in cardiomyocytes of type 2 diabetic rats.
Article Snippet: Determination of caspase-3 protease activity and myocardial apoptotic death The substrates Ac-DEVD-AFC, Ac-IETC-AFC, and Ac-LEHD-AFC were used, respectively, to determine the caspase (-3, -8, and -9) protease activity according to the manufacturer’s instructions (Biomol, Houston, USA) [16].

Article Title: Cardiac Specific Overexpression of Mitochondrial Omi/HtrA2 Induces Myocardial Apoptosis and Cardiac Dysfunction.
Article Snippet: The substrates Ac-DEVD-AFC, and Ac-LEHD-AFC were used to determine caspase-3, and caspase-9 activity respectively, following the manufacturer’s guidelines (BIOMOL, USA).

Article Title: Mesenchymal Stem Cell Survival in the Infarcted Heart Is Enhanced by Lentivirus Vector-Mediated Heat Shock Protein 27 Expression
Article Snippet: Caspase activation was also examined by a quantitative caspase-3-like enzyme activity assay using Ac-DEVD-AFC substrate (Biomol, Enzo Life Sciences) and staurosporine (STS; 500 n M for 12 hr) as a positive control.

Article Title: Modulation of neutrophil apoptosis by murine pulmonary microvascular endothelial cell inducible nitric oxide synthase.
Article Snippet: Neutrophils contribute significantly to ALI (acute lung injury) through adhesion to pulmonary microvascular endothelial cells (PMEC), trans-PMEC migration and alveolar infiltration.. Trans-PMEC migration delays expression of neutrophil apoptosis, which promotes intra-alveolar neutrophil survival and neutrophil mediated ALI.. We assessed the role of neutrophil vs PMEC inducible nitric oxide (NO) synthase (iNOS) in modulating neutrophil apoptosis.

Article Title: Lentiviral vector mediated modification of mesenchymal stem cells & enhanced survival in an in vitro model of ischaemia
Article Snippet: Caspase-3 activation was also examined using Ac-DEVD-AFC substrate (Biomol, Enzo Life Sciences, PA, USA).

Article Title: miR-145 induces caspase-dependent and -independent cell death in urothelial cancer cell lines with targeting of an expression signature present in Ta bladder tumors.
Article Snippet: The kinetic cleavage of the substrate Ac-DEVD-AFC (Biomol, Plymouth Meeting, PA, USA) as measured by the liberation of AFC (excitation, 400 nm; emission, 489 nm) was measured in cell lysates using a Synergy HT multi-mode micro plate reader, (Biotek Industries Inc., Winooski, VT, USA).

Activity Assay:

Article Title: Aβ(1-42) aggregates into non-toxic amyloid assemblies in the presence of the natural polyphenol oleuropein aglycon.
Article Snippet: Amyloid aggregation starts with the initial misfolding of peptide/protein precursors, with subsequent structural rearrangement into oligomers and protofibrils; the latter eventually organize into fibrils with shared basic structural features, found deposited in amyloid diseases.. Mounting evidence indicates early oligomers as the most toxic amyloid species; accordingly, the search of inhibitors of their growth is considered a promising target to prevent amyloid toxicity.. We recently showed that oleuropein aglycon, a polyphenol abundant in the extra virgin olive oil, interferes with the aggregation of amylin (involved in type-2 diabetes), eliminating its cytotoxicity.

Article Title: Variations of thioredoxin system contributes to increased susceptibility to apoptosis in cardiomyocytes of type 2 diabetic rats.
Article Snippet: Determination of caspase-3 protease activity and myocardial apoptotic death The substrates Ac-DEVD-AFC, Ac-IETC-AFC, and Ac-LEHD-AFC were used, respectively, to determine the caspase (-3, -8, and -9) protease activity according to the manufacturer’s instructions (Biomol, Houston, USA) [16].

Article Title: Cardiac Specific Overexpression of Mitochondrial Omi/HtrA2 Induces Myocardial Apoptosis and Cardiac Dysfunction.
Article Snippet: The substrates Ac-DEVD-AFC, and Ac-LEHD-AFC were used to determine caspase-3, and caspase-9 activity respectively, following the manufacturer’s guidelines (BIOMOL, USA).

Article Title: Mesenchymal Stem Cell Survival in the Infarcted Heart Is Enhanced by Lentivirus Vector-Mediated Heat Shock Protein 27 Expression
Article Snippet: Caspase activation was also examined by a quantitative caspase-3-like enzyme activity assay using Ac-DEVD-AFC substrate (Biomol, Enzo Life Sciences) and staurosporine (STS; 500 n M for 12 hr) as a positive control.

Article Title: Modulation of neutrophil apoptosis by murine pulmonary microvascular endothelial cell inducible nitric oxide synthase.
Article Snippet: Neutrophils contribute significantly to ALI (acute lung injury) through adhesion to pulmonary microvascular endothelial cells (PMEC), trans-PMEC migration and alveolar infiltration.. Trans-PMEC migration delays expression of neutrophil apoptosis, which promotes intra-alveolar neutrophil survival and neutrophil mediated ALI.. We assessed the role of neutrophil vs PMEC inducible nitric oxide (NO) synthase (iNOS) in modulating neutrophil apoptosis.

Article Title: Lentiviral vector mediated modification of mesenchymal stem cells & enhanced survival in an in vitro model of ischaemia
Article Snippet: Caspase-3 activation was also examined using Ac-DEVD-AFC substrate (Biomol, Enzo Life Sciences, PA, USA).

Article Title: miR-145 induces caspase-dependent and -independent cell death in urothelial cancer cell lines with targeting of an expression signature present in Ta bladder tumors.
Article Snippet: The kinetic cleavage of the substrate Ac-DEVD-AFC (Biomol, Plymouth Meeting, PA, USA) as measured by the liberation of AFC (excitation, 400 nm; emission, 489 nm) was measured in cell lysates using a Synergy HT multi-mode micro plate reader, (Biotek Industries Inc., Winooski, VT, USA).



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Image Search Results


Action of cetuximab-vc-MMAF on cell viability. A. Flow cytometry-based apoptosis assay of cells treated with the cetuximab-vc-MMAF (10 nM) for the indicated times. Live cells are shown in the lower left quadrant (Annexin V-/PI-), while the other three quadrants represent different stages of non-viable cells. B. Graphical representation of non-viable cells for each condition. Data are plotted as mean ± SD of three independent experiments. C. Western blot analysis of apoptosis-related proteins at the indicated time points following treatment with 10 nM cetuximab-vc-MMAF. Actin was used as a loading control, and molecular weight markers are shown at the right. D. Analysis of caspase-3 activity in cells treated with cetuximab-vc-MMAF (10 nM) for the indicated times. Data represent mean ± SD of fluorescence intensity resulting from substrate cleavage (Ac-DEVD-AFC) by activated caspase-3 in two independent experiments.

Journal: Neoplasia (New York, N.Y.)

Article Title: Rational payload selection enables high antitumoral efficacy of an anti-EGFR antibody-drug conjugate against ovarian tumors

doi: 10.1016/j.neo.2026.101295

Figure Lengend Snippet: Action of cetuximab-vc-MMAF on cell viability. A. Flow cytometry-based apoptosis assay of cells treated with the cetuximab-vc-MMAF (10 nM) for the indicated times. Live cells are shown in the lower left quadrant (Annexin V-/PI-), while the other three quadrants represent different stages of non-viable cells. B. Graphical representation of non-viable cells for each condition. Data are plotted as mean ± SD of three independent experiments. C. Western blot analysis of apoptosis-related proteins at the indicated time points following treatment with 10 nM cetuximab-vc-MMAF. Actin was used as a loading control, and molecular weight markers are shown at the right. D. Analysis of caspase-3 activity in cells treated with cetuximab-vc-MMAF (10 nM) for the indicated times. Data represent mean ± SD of fluorescence intensity resulting from substrate cleavage (Ac-DEVD-AFC) by activated caspase-3 in two independent experiments.

Article Snippet: The payloads used for antibodies conjugation, MC-GGFG-DXD (DXd, catalog reference: HY-13631E), MC-Val-Cit-PAB-MMAF (Vc-MMAF, catalog reference: HY-112786) and McMMAF (catalog reference: HY-15578), the belantamab mafodotin ADC (catalog reference: HY-P3239), as well as the Ac-DEVD-AFC (catalog reference: HY-P1005) substrate for caspase-3 activity detection, were purchased from MedChem Express (Princeton, NJ, USA).

Techniques: Flow Cytometry, Apoptosis Assay, Western Blot, Control, Molecular Weight, Activity Assay, Fluorescence