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synthetic fluorescence peptide devd-7-amino-4-trifluoromethyl coumarin (afc)  (MBL Life science)

 
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    Structured Review

    MBL Life science synthetic fluorescence peptide devd-7-amino-4-trifluoromethyl coumarin (afc)
    Synthetic Fluorescence Peptide Devd 7 Amino 4 Trifluoromethyl Coumarin (Afc), supplied by MBL Life science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ac-devd-afc+substrate+assay/fluorogenic+substrate+ac+devd+afc/us07390625-710-14-11
    Average 90 stars, based on 1 article reviews
    synthetic fluorescence peptide devd-7-amino-4-trifluoromethyl coumarin (afc) - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Activity Assay:

    Article Title: Development of a fluorescence peptide chip for the detection of caspase activity.
    Article Snippet: Proteases play a key role in cell functions, and it is very important to monitor their activities for drug screening and diagnosis of diseases.. In the present study, a new class of fluorescence probe, into which a fluorophore and a quencher have been introduced, was developed and applied to the on-chip detection of caspase-3 activity.. This probe is non fluorescent in the absence of caspase-3.

    other:

    Article Title: GMEB1, a novel endogenous caspase inhibitor, prevents hypoxia- and oxidative stress-induced neuronal apoptosis.
    Article Snippet: The interaction of glucoco or -9 prevents caspase oli GMEB1 on neuronal apop caspase activation and ap cating that it functions as stress.. We propose that G As the primary mediators of apoptosis, caspases cleave a variety of endogenous cytosolic and nuclear substrates [9,12,13].. Caspases are synthesized as inactive precursors called procaspases, and their activation is strictly controlled by a number of regulatory proteins, including Bcl-2, heat-shock proteins (HSPs), and the inhibitor of apoptosis (IAP) family [2,6,7,22,25,27].

    Article Title: Possible roles of neuropeptide Y Y3-receptor subtype in rat aortic endothelial cell proliferation under hypoxia, and its specific signal transduction.
    Article Snippet: Zhi-Yong Chen, Guo-Gang Feng, Kimitoshi Nishiwaki, Yasuhiro Shimada, Yoshihiro Fujiwara, Toru Komatsu, and Naohisa Ishikawa Department of Pharmacology, Aichi Medical University School of Medicine, Nagakute, Aichi; Department of Anesthesiology, Nagoya University School of Medicine, Showa-ku, Nagoya; and Department of Anesthesiology, Aichi Medical University School of Medicine, Nagakute, Aichi, Japan

    Article Title: Resveratrol induces cell death in colorectal cancer cells by a novel pathway involving lysosomal cathepsin D.
    Article Snippet: Caspase 3 activity was assayed with the fluorogenic substrate Asp-Glu-ValAsp-7-amino-4-trifluoromethyl coumarin following the manufacturer’s protocol (MBL, Naka-Ku, Nagoya, Japan).

    Article Title: Apoptosis-associated protein and use thereof
    Article Snippet: The measurement was conducted using a CPP32/caspase-3 fluorometric protease assay kit (MBL) with the synthetic fluorescence peptide DEVD-7-amino-4-trifluoromethyl coumarin (AFC) as a substrate.

    Article Title: Sonodynamically‐induced apoptosis, necrosis, and active oxygen generation by mono‐l‐aspartyl chlorin e6
    Article Snippet: Caspase‐3 activity was assayed using the specific fluorogenic substrate Ac‐DEVD‐AFC (MBL, Tokyo, Japan).

    Article Title: Sonodynamically-induced apoptosis, necrosis, and active oxygen generation by mono-l-aspartyl chlorin e6.
    Article Snippet: In this study, we investigated the induction of apoptosis by ultrasound in the presence of a photochemically active chlorine, mono-l-aspartyl chlorin e6 (NPe6).. HL-60 cells were exposed to ultrasound for up to 3 min in the presence and absence of NPe6, and the induction of apoptosis was examined by analyzing cell morphology, DNA fragmentation, and caspase-3 activity.. Cells treated with 80 μM NPe6 and ultrasound clearly showed membrane blebbing and cell shrinkage, whereas significant morphologic changes were not observed in cells exposed to either ultrasound alone, at the same intensity, or NPe6 alone.



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    Image Search Results


    Primary B-CLL cells (A, B) and B-cell cancer lines (C, D) treated with GSK-7975A (GSK, 10, 20, 50 μM) for 48 hours. Alive and dead cells were determined using LIVE/DEAD® staining Kits and analyzed by flow cytometry. (A, C) Representative flow cytometry plots. (B, D) Quantification of the percentage of dead cells in (A, C). Cell mortality was also determined by flow cytometry after annexin V/PI staining (E, F). The percentage of total apoptotic cells was calculated as the percentage of annexin V-FITC positive plus annexin V-FITC/PI-positive population. (G, H) Caspase-3 activity determined using Ac-DEVD-AFC as substrate after 24 hours with GSK-7975A (GSK, 0-50 μM) or Synta66 (S66, 0-50 μM) in primary B-CLL cells and B-cell cancer lines. Data from B-cell cancer lines are representative of at least 3 independent experiments. Statistical significance was analyzed by two-tailed, unpaired Student’s t test.

    Journal: bioRxiv

    Article Title: Blocking Orai1 constitutive activity inhibits B-cell cancer migration and synergistically acts with drugs to reduce B-CLL cell survival

    doi: 10.1101/2023.12.12.571243

    Figure Lengend Snippet: Primary B-CLL cells (A, B) and B-cell cancer lines (C, D) treated with GSK-7975A (GSK, 10, 20, 50 μM) for 48 hours. Alive and dead cells were determined using LIVE/DEAD® staining Kits and analyzed by flow cytometry. (A, C) Representative flow cytometry plots. (B, D) Quantification of the percentage of dead cells in (A, C). Cell mortality was also determined by flow cytometry after annexin V/PI staining (E, F). The percentage of total apoptotic cells was calculated as the percentage of annexin V-FITC positive plus annexin V-FITC/PI-positive population. (G, H) Caspase-3 activity determined using Ac-DEVD-AFC as substrate after 24 hours with GSK-7975A (GSK, 0-50 μM) or Synta66 (S66, 0-50 μM) in primary B-CLL cells and B-cell cancer lines. Data from B-cell cancer lines are representative of at least 3 independent experiments. Statistical significance was analyzed by two-tailed, unpaired Student’s t test.

    Article Snippet: As reported and described previously [ ], activity of caspase-3 was performed by using Ac-DEVD-AFC substrate (Enzo Life Sciences, France).

    Techniques: Staining, Flow Cytometry, Activity Assay, Two Tailed Test