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scc human skin tissues  (Novus Biologicals)


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    Novus Biologicals scc human skin tissues
    Scc Human Skin Tissues, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 85/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/Microarrays/Human+Skin+Tissue+MicroArray+(Cancer)/pm40441291-283-24-29
    Average 85 stars, based on 9 article reviews
    scc human skin tissues - by Bioz Stars, 2026-10
    85/100 stars

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    Related Articles

    Immunohistochemical staining:

    Article Title: Up-Regulation of the Lymphatic Marker Podoplanin, a Mucin-Type Transmembrane Glycoprotein, in Human Squamous Cell Carcinomas and Germ Cell Tumors
    Article Snippet: Immunofluorescence stainings were performed on 6-μm cryostat sections of neonatal human foreskin or on 6-μm paraffin sections of human malignant melanoma as described previously, 6,10 using the mouse monoclonal antibody D2-40 (Signet, Dedham, MA), rabbit polyclonal antibodies against the lymphatic markers LYVE-1 7 and Prox1 25 (kindly provided by Dr. K. Alitalo, University of Helsinki, Helsinki, Finland), CD34, CD31 (BD Pharmingen, San Diego, CA), and corresponding secondary antibodies labeled with Alexa Fluor 488 or Alexa Fluor 594 (Molecular Probes, Eugene, OR). .. Additional immunohistochemical stains were performed on tissue arrays of normal mouse (MaxArray mouse tissue microarray slides; Zymed, San Francisco, CA) and human tissues (MaxArray human normal tissue microarray slides, Zymed), human skin tumors (IMH-323; Imgenex, San Diego, CA) and ovary tumors (IMH-347, Imgenex) as described previously. .. 6 Briefly, the primary antibodies D2-40 or LYVE-1 were applied, followed by incubation with conjugated anti-mouse or anti-rabbit immunoglobulin using the 3-amino-9-ethylcabazole peroxidase kit (Vector Laboratories, Burlingame, CA).

    Microarray:

    Article Title: Up-Regulation of the Lymphatic Marker Podoplanin, a Mucin-Type Transmembrane Glycoprotein, in Human Squamous Cell Carcinomas and Germ Cell Tumors
    Article Snippet: Immunofluorescence stainings were performed on 6-μm cryostat sections of neonatal human foreskin or on 6-μm paraffin sections of human malignant melanoma as described previously, 6,10 using the mouse monoclonal antibody D2-40 (Signet, Dedham, MA), rabbit polyclonal antibodies against the lymphatic markers LYVE-1 7 and Prox1 25 (kindly provided by Dr. K. Alitalo, University of Helsinki, Helsinki, Finland), CD34, CD31 (BD Pharmingen, San Diego, CA), and corresponding secondary antibodies labeled with Alexa Fluor 488 or Alexa Fluor 594 (Molecular Probes, Eugene, OR). .. Additional immunohistochemical stains were performed on tissue arrays of normal mouse (MaxArray mouse tissue microarray slides; Zymed, San Francisco, CA) and human tissues (MaxArray human normal tissue microarray slides, Zymed), human skin tumors (IMH-323; Imgenex, San Diego, CA) and ovary tumors (IMH-347, Imgenex) as described previously. .. 6 Briefly, the primary antibodies D2-40 or LYVE-1 were applied, followed by incubation with conjugated anti-mouse or anti-rabbit immunoglobulin using the 3-amino-9-ethylcabazole peroxidase kit (Vector Laboratories, Burlingame, CA).

    Proximity Ligation Assay:

    Article Title: Mitochondrial Raf1 Regulates Glutamine Catabolism
    Article Snippet: .. 736 737 Proximity Ligation Assay (PLA) of tumor tissue microarrays 738 Deparaffinization 739 Proximity ligation of human cancer tissue microarrays (Skin cancer: NBP2-30229, Multiple 740 cancers: NBP2-30263; Novus Biologicals) was used to analyze Raf-GLS protein-protein 741 interaction in diverse cancer contexts. ..

    Ligation:

    Article Title: Mitochondrial Raf1 Regulates Glutamine Catabolism
    Article Snippet: .. 736 737 Proximity Ligation Assay (PLA) of tumor tissue microarrays 738 Deparaffinization 739 Proximity ligation of human cancer tissue microarrays (Skin cancer: NBP2-30229, Multiple 740 cancers: NBP2-30263; Novus Biologicals) was used to analyze Raf-GLS protein-protein 741 interaction in diverse cancer contexts. ..

    other:

    Article Title: Up-Regulation of the Lymphatic Marker Podoplanin, a Mucin-Type Transmembrane Glycoprotein, in Human Squamous Cell Carcinomas and Germ Cell Tumors
    Article Snippet: Nuclei were counterstained with 20 g/ml of Hoechst bisbenzimide (Molecular Probes).

    RNAscope:

    Article Title: In Vivo CRISPR Interference Screen Reveals Long Noncoding RNA Portfolio Crucial for Cutaneous Squamous Cell Carcinoma Tumor Growth.
    Article Snippet: .. Cox proportional hazards regression model fitting cor- rected for sex bias was performed using the survival and adjust- edCurves R packages. smRNA-FISH Healthy and SCC human skin tissues (NBP2-30229, Novus Bi- ologicals) were processed for smRNA-FISH as previously described in Ducoli et al (2021), following the manufacturer’s instruction on the RNAScope Multiplex Fluorescent Reagent kit v2 (Advanced Cell Diagnostics). .. The following RNAscope probes were used: Hs- LINC00704-C1 (411081-C1, Advanced Cell Diagnostics) and Hs- Malat1-C2 (400811-C2, Advanced Cell Diagnostics; as positive control).

    Multiplex Assay:

    Article Title: In Vivo CRISPR Interference Screen Reveals Long Noncoding RNA Portfolio Crucial for Cutaneous Squamous Cell Carcinoma Tumor Growth.
    Article Snippet: .. Cox proportional hazards regression model fitting cor- rected for sex bias was performed using the survival and adjust- edCurves R packages. smRNA-FISH Healthy and SCC human skin tissues (NBP2-30229, Novus Bi- ologicals) were processed for smRNA-FISH as previously described in Ducoli et al (2021), following the manufacturer’s instruction on the RNAScope Multiplex Fluorescent Reagent kit v2 (Advanced Cell Diagnostics). .. The following RNAscope probes were used: Hs- LINC00704-C1 (411081-C1, Advanced Cell Diagnostics) and Hs- Malat1-C2 (400811-C2, Advanced Cell Diagnostics; as positive control).



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    The VANC-D4 assay (A) Fabrication of the VANC-D4 assay. SI-ATRP is used to grow POEGMA-co-GMA brushes on glass slides. Following SI-ATRP, VANC-BSA is <t>inkjet</t> printed into the polymer brush to create stable spots and fluorescently labeled anti-VANC antibodies (termed dAb) are printed on trehalose pads to create labile spots. (B) Sequential steps of the VANC-D4 assay. The steps of the VANC-D4 are (i) dispensing a blood sample on the surface, (ii) dissolution of the trehalose pads to release the fluorescently labeled dAb into solution, (iii) diffusion of the labeled dAb in the sample volume, (iv) detection of fluorescence signal from the antibody bound to immobilized VANC-BSA. (C) Representative VANC-D4 assay response in the presence of (i) no drug, (ii) low drug, and (iii) high drug concentration. (D) VANC monitoring across its clinical range using VANC-spiked whole blood diluted 100-fold in running buffer, analyzed in triplicate. The clinical range for the test is 1–45 μg/mL. Intensity values are shown, with error bars that represent the standard error of the means for n = 3 separate runs. The LOD for the presented dose-response curve was calculated to be 0.8 μg/mL.
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    Image Search Results


    The VANC-D4 assay (A) Fabrication of the VANC-D4 assay. SI-ATRP is used to grow POEGMA-co-GMA brushes on glass slides. Following SI-ATRP, VANC-BSA is inkjet printed into the polymer brush to create stable spots and fluorescently labeled anti-VANC antibodies (termed dAb) are printed on trehalose pads to create labile spots. (B) Sequential steps of the VANC-D4 assay. The steps of the VANC-D4 are (i) dispensing a blood sample on the surface, (ii) dissolution of the trehalose pads to release the fluorescently labeled dAb into solution, (iii) diffusion of the labeled dAb in the sample volume, (iv) detection of fluorescence signal from the antibody bound to immobilized VANC-BSA. (C) Representative VANC-D4 assay response in the presence of (i) no drug, (ii) low drug, and (iii) high drug concentration. (D) VANC monitoring across its clinical range using VANC-spiked whole blood diluted 100-fold in running buffer, analyzed in triplicate. The clinical range for the test is 1–45 μg/mL. Intensity values are shown, with error bars that represent the standard error of the means for n = 3 separate runs. The LOD for the presented dose-response curve was calculated to be 0.8 μg/mL.

    Journal: iScience

    Article Title: A rapid point-of-care test for vancomycin monitoring at the clinical bedside

    doi: 10.1016/j.isci.2026.116064

    Figure Lengend Snippet: The VANC-D4 assay (A) Fabrication of the VANC-D4 assay. SI-ATRP is used to grow POEGMA-co-GMA brushes on glass slides. Following SI-ATRP, VANC-BSA is inkjet printed into the polymer brush to create stable spots and fluorescently labeled anti-VANC antibodies (termed dAb) are printed on trehalose pads to create labile spots. (B) Sequential steps of the VANC-D4 assay. The steps of the VANC-D4 are (i) dispensing a blood sample on the surface, (ii) dissolution of the trehalose pads to release the fluorescently labeled dAb into solution, (iii) diffusion of the labeled dAb in the sample volume, (iv) detection of fluorescence signal from the antibody bound to immobilized VANC-BSA. (C) Representative VANC-D4 assay response in the presence of (i) no drug, (ii) low drug, and (iii) high drug concentration. (D) VANC monitoring across its clinical range using VANC-spiked whole blood diluted 100-fold in running buffer, analyzed in triplicate. The clinical range for the test is 1–45 μg/mL. Intensity values are shown, with error bars that represent the standard error of the means for n = 3 separate runs. The LOD for the presented dose-response curve was calculated to be 0.8 μg/mL.

    Article Snippet: Following polymerization, D4 assays were fabricated using non-contact inkjet printing methods as described elsewhere., VANC-BSA was printed using a non-contact inkjet microarray printer (Scienion, sciFLEXARRAYER Type S12).

    Techniques: Polymer, Labeling, Dissolution, Diffusion-based Assay, Fluorescence, Concentration Assay