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adult normal human brain tissue lysates  (Novus Biologicals)


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    Structured Review

    Novus Biologicals adult normal human brain tissue lysates
    a Comparison of CHSY1 gene expression in glioma subtypes and <t>normal</t> <t>brain</t> <t>tissue</t> in the REMBRANDT glioma microarray database. ** P < 0.01, **** P < 0.0001. b High expression of CHSY1 was associated with worse overall survival in glioma patients. The high and low expression groups were divided by median expression level of CHSY1 in 329 cases. These data were from the REMBRANDT database ( http://www.betastasis.com/glioma/rembrandt/ ). c Immunohistochemistry of CHSY1 (upper panel) and CS56 (lower panel) on tissue array contains 85 primary glioma cases. The staining was visualized in brown color with a 3,3-diaminobenzidine liquid substrate system. All sections were counterstained with hematoxylin. Representative images of four glioma cases with different staining intensities are shown. Amplified images are shown at the bottom right of each image. Scale bars, 50 μm. Arrows indicate positive stained glioma cells. d Representative images of CHSY1 staining on normal brain tissue ( n = 5). e Statistical analysis of immunohistochemistry in glioma tissue array. Mann–Whitney U -test was used. P -values are shown at top. f Expression of CHSY1 in glioma cell lines and normal <t>human</t> brain tissue. The protein expression was analyzed by western blotting. Total loading protein is shown at bottom. Relative expression levels to total brain tissue form three independent blots are shown at the right.
    Adult Normal Human Brain Tissue Lysates, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/Microarrays/Human+Brain+Tissue+MicroArray+(Normal)/pmc07000683-223-0-9
    Average 93 stars, based on 2 article reviews
    adult normal human brain tissue lysates - by Bioz Stars, 2026-10
    93/100 stars

    Images

    1) Product Images from "Chondroitin sulfate synthase 1 enhances proliferation of glioblastoma by modulating PDGFRA stability"

    Article Title: Chondroitin sulfate synthase 1 enhances proliferation of glioblastoma by modulating PDGFRA stability

    Journal: Oncogenesis

    doi: 10.1038/s41389-020-0197-0

    a Comparison of CHSY1 gene expression in glioma subtypes and normal brain tissue in the REMBRANDT glioma microarray database. ** P < 0.01, **** P < 0.0001. b High expression of CHSY1 was associated with worse overall survival in glioma patients. The high and low expression groups were divided by median expression level of CHSY1 in 329 cases. These data were from the REMBRANDT database ( http://www.betastasis.com/glioma/rembrandt/ ). c Immunohistochemistry of CHSY1 (upper panel) and CS56 (lower panel) on tissue array contains 85 primary glioma cases. The staining was visualized in brown color with a 3,3-diaminobenzidine liquid substrate system. All sections were counterstained with hematoxylin. Representative images of four glioma cases with different staining intensities are shown. Amplified images are shown at the bottom right of each image. Scale bars, 50 μm. Arrows indicate positive stained glioma cells. d Representative images of CHSY1 staining on normal brain tissue ( n = 5). e Statistical analysis of immunohistochemistry in glioma tissue array. Mann–Whitney U -test was used. P -values are shown at top. f Expression of CHSY1 in glioma cell lines and normal human brain tissue. The protein expression was analyzed by western blotting. Total loading protein is shown at bottom. Relative expression levels to total brain tissue form three independent blots are shown at the right.
    Figure Legend Snippet: a Comparison of CHSY1 gene expression in glioma subtypes and normal brain tissue in the REMBRANDT glioma microarray database. ** P < 0.01, **** P < 0.0001. b High expression of CHSY1 was associated with worse overall survival in glioma patients. The high and low expression groups were divided by median expression level of CHSY1 in 329 cases. These data were from the REMBRANDT database ( http://www.betastasis.com/glioma/rembrandt/ ). c Immunohistochemistry of CHSY1 (upper panel) and CS56 (lower panel) on tissue array contains 85 primary glioma cases. The staining was visualized in brown color with a 3,3-diaminobenzidine liquid substrate system. All sections were counterstained with hematoxylin. Representative images of four glioma cases with different staining intensities are shown. Amplified images are shown at the bottom right of each image. Scale bars, 50 μm. Arrows indicate positive stained glioma cells. d Representative images of CHSY1 staining on normal brain tissue ( n = 5). e Statistical analysis of immunohistochemistry in glioma tissue array. Mann–Whitney U -test was used. P -values are shown at top. f Expression of CHSY1 in glioma cell lines and normal human brain tissue. The protein expression was analyzed by western blotting. Total loading protein is shown at bottom. Relative expression levels to total brain tissue form three independent blots are shown at the right.

    Techniques Used: Comparison, Gene Expression, Microarray, Expressing, Immunohistochemistry, Staining, Amplification, MANN-WHITNEY, Western Blot

    Related Articles

    In Situ Hybridization:

    Article Title: FIN-Seq: transcriptional profiling of specific cell types from frozen archived tissue of the human central nervous system
    Article Snippet: FFPE adult human cerebral cortex tissue from a 54-year-old female was obtained from Abcam (ab4296). .. Chromogenic double in situ hybridization was performed for the human brain tissue using the RNAscope 2.5 HD Duplex Assay (Advanced Cell Diagnostics) according to the manufacturer's protocol. .. A Fluorescent Multiplex assay was used for the human retina tissue according to the manufacturer's protocol (Advanced Cell Diagnostics).

    RNAscope:

    Article Title: FIN-Seq: transcriptional profiling of specific cell types from frozen archived tissue of the human central nervous system
    Article Snippet: FFPE adult human cerebral cortex tissue from a 54-year-old female was obtained from Abcam (ab4296). .. Chromogenic double in situ hybridization was performed for the human brain tissue using the RNAscope 2.5 HD Duplex Assay (Advanced Cell Diagnostics) according to the manufacturer's protocol. .. A Fluorescent Multiplex assay was used for the human retina tissue according to the manufacturer's protocol (Advanced Cell Diagnostics).

    Article Title: Transcriptomic analysis of the human habenula in schizophrenia
    Article Snippet: .. smFISH experiments were performed on postmortem human brain tissue using RNAScope Multiplex Fluorescent Reagent Kit v2 (Advanced Cell Diagnostics) and 4-Plex Ancillary Kit as previously described . ..

    Labeling:

    Article Title: Isoglutaminyl cyclase contributes to CCL2-driven neuroinflammation in Alzheimer’s disease
    Article Snippet: .. In human brain tissue, the goat antiserum sc-1784 and the mouse monoclonal antibody MAB2791 (R&D Systems) demonstrated distinct neuronal CCL2 labeling comparable to the staining pattern observed in mouse brain. ..

    Staining:

    Article Title: Isoglutaminyl cyclase contributes to CCL2-driven neuroinflammation in Alzheimer’s disease
    Article Snippet: .. In human brain tissue, the goat antiserum sc-1784 and the mouse monoclonal antibody MAB2791 (R&D Systems) demonstrated distinct neuronal CCL2 labeling comparable to the staining pattern observed in mouse brain. ..

    Multiplex Assay:

    Article Title: Transcriptomic analysis of the human habenula in schizophrenia
    Article Snippet: .. smFISH experiments were performed on postmortem human brain tissue using RNAScope Multiplex Fluorescent Reagent Kit v2 (Advanced Cell Diagnostics) and 4-Plex Ancillary Kit as previously described . ..

    other:

    Article Title: FIN-Seq: Transcriptional profiling of specific cell types in frozen archived tissue from the human central nervous system
    Article Snippet: FFPE adult human cerebral cortex tissue from a 54-year-old female was obtained from Abcam (ab4296).



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    Image Search Results


    The VANC-D4 assay (A) Fabrication of the VANC-D4 assay. SI-ATRP is used to grow POEGMA-co-GMA brushes on glass slides. Following SI-ATRP, VANC-BSA is inkjet printed into the polymer brush to create stable spots and fluorescently labeled anti-VANC antibodies (termed dAb) are printed on trehalose pads to create labile spots. (B) Sequential steps of the VANC-D4 assay. The steps of the VANC-D4 are (i) dispensing a blood sample on the surface, (ii) dissolution of the trehalose pads to release the fluorescently labeled dAb into solution, (iii) diffusion of the labeled dAb in the sample volume, (iv) detection of fluorescence signal from the antibody bound to immobilized VANC-BSA. (C) Representative VANC-D4 assay response in the presence of (i) no drug, (ii) low drug, and (iii) high drug concentration. (D) VANC monitoring across its clinical range using VANC-spiked whole blood diluted 100-fold in running buffer, analyzed in triplicate. The clinical range for the test is 1–45 μg/mL. Intensity values are shown, with error bars that represent the standard error of the means for n = 3 separate runs. The LOD for the presented dose-response curve was calculated to be 0.8 μg/mL.

    Journal: iScience

    Article Title: A rapid point-of-care test for vancomycin monitoring at the clinical bedside

    doi: 10.1016/j.isci.2026.116064

    Figure Lengend Snippet: The VANC-D4 assay (A) Fabrication of the VANC-D4 assay. SI-ATRP is used to grow POEGMA-co-GMA brushes on glass slides. Following SI-ATRP, VANC-BSA is inkjet printed into the polymer brush to create stable spots and fluorescently labeled anti-VANC antibodies (termed dAb) are printed on trehalose pads to create labile spots. (B) Sequential steps of the VANC-D4 assay. The steps of the VANC-D4 are (i) dispensing a blood sample on the surface, (ii) dissolution of the trehalose pads to release the fluorescently labeled dAb into solution, (iii) diffusion of the labeled dAb in the sample volume, (iv) detection of fluorescence signal from the antibody bound to immobilized VANC-BSA. (C) Representative VANC-D4 assay response in the presence of (i) no drug, (ii) low drug, and (iii) high drug concentration. (D) VANC monitoring across its clinical range using VANC-spiked whole blood diluted 100-fold in running buffer, analyzed in triplicate. The clinical range for the test is 1–45 μg/mL. Intensity values are shown, with error bars that represent the standard error of the means for n = 3 separate runs. The LOD for the presented dose-response curve was calculated to be 0.8 μg/mL.

    Article Snippet: Following polymerization, D4 assays were fabricated using non-contact inkjet printing methods as described elsewhere., VANC-BSA was printed using a non-contact inkjet microarray printer (Scienion, sciFLEXARRAYER Type S12).

    Techniques: Polymer, Labeling, Dissolution, Diffusion-based Assay, Fluorescence, Concentration Assay