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5x amv reverse transcriptase reaction buffer  (Promega)

 
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    Promega 5x amv reverse transcriptase reaction buffer
    5x Amv Reverse Transcriptase Reaction Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/5x+prime+script+reverse+transcriptase/amv+reverse+transcriptase+buffer/pmc07533451-74-38-50
    Average 90 stars, based on 1 article reviews
    5x amv reverse transcriptase reaction buffer - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Identification of σ E -Dependent Promoter Upstream of clpB from the Pathogenic Spirochaete Leptospira interrogans by Applying an E. coli Two-Plasmid System
    Article Snippet: Briefly, 2 μg of total RNA were combined with 0.6 pmol of a primer labeled at the 5’-terminus with [γ- 32 P]ATP (specific activity 6000 Ci/mmol; Hartmann) using polynucleotide kinase (Promega), denatured at 75 °C for 10 min, and annealed at 58 °C for 20 min. .. This was followed by the addition of extension buffer (1x AMV reverse transcriptase buffer (Promega, Medison, WI, USA), 1 mM dNTPs, 20 U RNase-In (Promega), and 10 U of the AMV reverse transcriptase (Promega). ..

    Article Title: TRMT1L-catalyzed m 2 2 G27 on tyrosine tRNA is required for efficient mRNA translation and cell survival under oxidative stress
    Article Snippet: AMV Reverse Transcriptase , Promega , M510A. .. AMV Reverse Transcriptase 5X Reaction Buffer , Promega , M515A. ..

    Article Title: Nrf2 downregulates zymosan-induced neutrophil activation and modulates migration
    Article Snippet: The reaction used 1 μg of total RNA, a dNTP mixture (containing 25 mM dATP, dGTP, dCTP, and dTTP) and 50 μM oligo (dT) primers (MWG Biotech, Ebersberg, Germany). .. Reverse transcription was performed in 1× AMV reverse transcriptase reaction buffer (Promega, Charbonnières-les-Bains, France), with RNase inhibitor (RNasine; Promega) at 40 U/μl, AMV reverse transcriptase (Promega) at 10 U/μl, and RNase-free water, to a final volume of 10 μl. ..

    Article Title: Pinealectomy increases thermogenesis and decreases lipogenesis
    Article Snippet: Total RNA was extracted with Trizol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol. .. For RT-PCR, 5 μg of total RNA were reverse transcribed for 30 min at 37°C in a reaction mixture containing RNA, 40 U RNase inhibitor (Promega, Madison, WI, USA), 0.5 mM deoxynucleotide triphosphate (Promega, Madison, WI, USA),2 μM random hexamer primers, 5 x AMV reverse transcriptase reaction buffer and 30 U AMV reverse transcriptase (Promega, Madison, WI, USA). .. Real-time RT-PCR analysis was performed using the SYBR Green PCR Master Mix (TOYOBO, Osaka, Japan) and real-time RT-PCR parameters were used as follows: initial denaturation 95°C for 30 s, amplification 45 cycles were performed at 95°C for 5 s, 60°C for 10 s, and 72°C for 15 s, melting curve 95°C for 10 s, 65°C for 1 min, cooling 37°C for 10 min with gene-specific primers.

    Article Title: The immunomodulatory effects of social isolation in mice are linked to temperature control.
    Article Snippet: Total RNA was extracted from blood (n = 6 for each housing group) using the RNeasy Protect Animal Blood Kit (Qiagen) according to the manufacturer’s protocol. .. Reverse transcription of the extracted RNA was performed using 1 μl of 0.5 μg/μl OLIGO dT primers (Promega) and 1 μl of RNase free-water and incubating samples at 70 ◦C for 10 min and cooling for 5 min. 5x AMV reverse transcriptase buffer, RNAsinPlus, RNase free water, 10 mM dnTP mix and AMV reverse transcriptase (all Promega) was added, and samples were incubated at 42 ◦C for 60 min and 10 min at 70 ◦C. .. Samples were loaded in triplicate and were run according to the manufacturer’s settings on a BioRad CFX Connect RTPCR machine using power SYBR Green master mix (Thermo Fisher Scientific) and QuantiTech primers (Qiagen).

    Article Title: Genome Sequencing of Historical Encephalomyocarditis Viruses from South Africa Links the Historical 1993/4 Savanna Elephant ( Loxodonta africana ) Outbreak to Cryptic Mastomys Rodents
    Article Snippet: .. Briefly, 4.5 μL of the viral RNA template was reverse transcribed in the presence of AMV Reverse Transcriptase reaction buffer (1X, Promega Corporation, Madison, WI, USA), 9μM random hexamers (Promega Corporation, Madison, WI, USA), 0.4 μM dNTPs (Fermentas, Waltham, MA, USA), 10 U Recombinant RNasin ® Ribonuclease inhibitor (Promega Corporation, Madison, WI, USA) and 5% DMSO in a final reaction volume of 9 μL. .. Following an initial denaturation step of 80 °C for 3 min, samples were snap-frozen in liquid nitrogen, transferred to ice, and 10 U of AMV Reverse Transcriptase (Promega Corporation, Madison, WI, USA) and at least 1 U of RNasin were added to each reaction, prior to a one-hour incubation at 42 °C.

    Article Title: Pinealectomy increases thermogenesis and decreases lipogenesis
    Article Snippet: Total RNA was extracted with TRIzol ® reagent (Invitrogen; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol. .. For RT-qPCR, 5 μg total RNA was reverse transcribed for 30 min at 37°C in a reaction mixture containing the RNA, 40 U RNase inhibitor (Promega Corporation), 0.5 mM deoxynucleotide triphosphate (Promega Corporation), 2 μM random hexamer primers, 5X AMV reverse transcriptase reaction buffer and 30 U AMV reverse transcriptase (Promega Corporation). .. RT-qPCR analysis was performed using the SYBR Green PCR Master Mix (Toyobo Life Science) and RT-qPCR thermocycling conditions were as follows: Initial denaturation at 95°C for 30 sec, followed by amplification for 45 cycles performed at 95°C for 5 sec, 60°C for 10 sec and 72°C for 15 sec, 95°C for 10 sec and 10 sec each at 0.2°C increments to 62°C for a melting curve analysis, and 65°C for 1 min, followed by cooling to 37°C for 10 min with gene-specific primers.

    Article Title: The Immunomodulatory Effects of Social Isolation in Mice are Linked to Temperature Control
    Article Snippet: Reverse transcription of the extracted RNA was performed using 1μl of 0.5μg/μl OLIGO dT primers (Promega) and 1μl of RNase free-water and incubating samples at 70°C for 10 minutes and cooling for 5 minutes. .. 5x AMV reverse transcriptase buffer, RNAsinPlus, RNase free water, 10mM dnTP mix and AMV reverse transcriptase (all Promega) was added, and samples were incubated at 42°C for 60 minutes and 10 minutes at 70°C. .. Samples were loaded in triplicate and were run according to the manufacturer’s settings on a BioRad CFX Connect RT-PCR machine using power SYBR Green master mix (Thermo Fisher Scientific) and QuantiTech primers (Qiagen).

    Random Hexamer:

    Article Title: Pinealectomy increases thermogenesis and decreases lipogenesis
    Article Snippet: Total RNA was extracted with Trizol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol. .. For RT-PCR, 5 μg of total RNA were reverse transcribed for 30 min at 37°C in a reaction mixture containing RNA, 40 U RNase inhibitor (Promega, Madison, WI, USA), 0.5 mM deoxynucleotide triphosphate (Promega, Madison, WI, USA),2 μM random hexamer primers, 5 x AMV reverse transcriptase reaction buffer and 30 U AMV reverse transcriptase (Promega, Madison, WI, USA). .. Real-time RT-PCR analysis was performed using the SYBR Green PCR Master Mix (TOYOBO, Osaka, Japan) and real-time RT-PCR parameters were used as follows: initial denaturation 95°C for 30 s, amplification 45 cycles were performed at 95°C for 5 s, 60°C for 10 s, and 72°C for 15 s, melting curve 95°C for 10 s, 65°C for 1 min, cooling 37°C for 10 min with gene-specific primers.

    Article Title: Pinealectomy increases thermogenesis and decreases lipogenesis
    Article Snippet: Total RNA was extracted with TRIzol ® reagent (Invitrogen; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol. .. For RT-qPCR, 5 μg total RNA was reverse transcribed for 30 min at 37°C in a reaction mixture containing the RNA, 40 U RNase inhibitor (Promega Corporation), 0.5 mM deoxynucleotide triphosphate (Promega Corporation), 2 μM random hexamer primers, 5X AMV reverse transcriptase reaction buffer and 30 U AMV reverse transcriptase (Promega Corporation). .. RT-qPCR analysis was performed using the SYBR Green PCR Master Mix (Toyobo Life Science) and RT-qPCR thermocycling conditions were as follows: Initial denaturation at 95°C for 30 sec, followed by amplification for 45 cycles performed at 95°C for 5 sec, 60°C for 10 sec and 72°C for 15 sec, 95°C for 10 sec and 10 sec each at 0.2°C increments to 62°C for a melting curve analysis, and 65°C for 1 min, followed by cooling to 37°C for 10 min with gene-specific primers.

    Incubation:

    Article Title: The immunomodulatory effects of social isolation in mice are linked to temperature control.
    Article Snippet: Total RNA was extracted from blood (n = 6 for each housing group) using the RNeasy Protect Animal Blood Kit (Qiagen) according to the manufacturer’s protocol. .. Reverse transcription of the extracted RNA was performed using 1 μl of 0.5 μg/μl OLIGO dT primers (Promega) and 1 μl of RNase free-water and incubating samples at 70 ◦C for 10 min and cooling for 5 min. 5x AMV reverse transcriptase buffer, RNAsinPlus, RNase free water, 10 mM dnTP mix and AMV reverse transcriptase (all Promega) was added, and samples were incubated at 42 ◦C for 60 min and 10 min at 70 ◦C. .. Samples were loaded in triplicate and were run according to the manufacturer’s settings on a BioRad CFX Connect RTPCR machine using power SYBR Green master mix (Thermo Fisher Scientific) and QuantiTech primers (Qiagen).

    Article Title: The Immunomodulatory Effects of Social Isolation in Mice are Linked to Temperature Control
    Article Snippet: Reverse transcription of the extracted RNA was performed using 1μl of 0.5μg/μl OLIGO dT primers (Promega) and 1μl of RNase free-water and incubating samples at 70°C for 10 minutes and cooling for 5 minutes. .. 5x AMV reverse transcriptase buffer, RNAsinPlus, RNase free water, 10mM dnTP mix and AMV reverse transcriptase (all Promega) was added, and samples were incubated at 42°C for 60 minutes and 10 minutes at 70°C. .. Samples were loaded in triplicate and were run according to the manufacturer’s settings on a BioRad CFX Connect RT-PCR machine using power SYBR Green master mix (Thermo Fisher Scientific) and QuantiTech primers (Qiagen).

    Recombinant:

    Article Title: Genome Sequencing of Historical Encephalomyocarditis Viruses from South Africa Links the Historical 1993/4 Savanna Elephant ( Loxodonta africana ) Outbreak to Cryptic Mastomys Rodents
    Article Snippet: .. Briefly, 4.5 μL of the viral RNA template was reverse transcribed in the presence of AMV Reverse Transcriptase reaction buffer (1X, Promega Corporation, Madison, WI, USA), 9μM random hexamers (Promega Corporation, Madison, WI, USA), 0.4 μM dNTPs (Fermentas, Waltham, MA, USA), 10 U Recombinant RNasin ® Ribonuclease inhibitor (Promega Corporation, Madison, WI, USA) and 5% DMSO in a final reaction volume of 9 μL. .. Following an initial denaturation step of 80 °C for 3 min, samples were snap-frozen in liquid nitrogen, transferred to ice, and 10 U of AMV Reverse Transcriptase (Promega Corporation, Madison, WI, USA) and at least 1 U of RNasin were added to each reaction, prior to a one-hour incubation at 42 °C.



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