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Promega 5x colorless gotaq ®flexi® buffer
5x Colorless Gotaq ®Flexi® Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/5x+goscripttm+buffer/pcr+buffer/pmc10590933-198-4-9
Average 90 stars, based on 1 article reviews
5x colorless gotaq ®flexi® buffer - by Bioz Stars, 2026-09
90/100 stars

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Article Title: HDAC1 controls the generation and maintenance of effector-like CD8 + T cells during chronic viral infection
Article Snippet: Cells were fixed with 25% formaldehyde (Sigma-Aldrich) in PBS (Sigma-Aldrich), permeabilized with 1% Triton X-100/PBS (Promega), and blocked with 5% FBS/PBS.

Article Title: 5′ untranslated regions tune Toxoplasma translation
Article Snippet: 1 mL lysis buffer (20 mM HEPES pH 7.5, 100 mM KCl, 5 mM MgCl 2 , 1% Triton X-100, 2 mM DTT, 100 ug mL -1 cycloheximide, 500 U mL -1 RNaseIn Plus (Promega), and cOmplete Protease Inhibitor Cocktail (1 tablet per 10 mL buffer, Roche)) was added, and a cell lifter was used to dissociate HFFs with intracellular parasites from the dish, combining both dishes into the 1 mL sample.

Article Title: MEG3 Enhances Survival of Developing Human Neurons with CLCN4 -Linked Autophagy Impairment
Article Snippet: Sections were permeabilized and blocked in PBS containing 0.1% Triton X-100 (Promega, H5141) and 5% donkey serum (Abcam, Ab7475) for 1 hour at RT.

Article Title: Molecular detection and characterisation of Toxoplasma gondii in eastern barred bandicoots ( Perameles gunnii ) in Victoria, Australia.
Article Snippet: Australian marsupials are particularly susceptible to Toxoplasma gondii, an introduced zoonotic protozoan parasite.. Molecular diagnostic methods are a highly specific approach for the detection of parasitic infections such as T. gondii.. Importantly, molecular methods are useful for the characterisation of T. gondii to understand the genetic diversity of the parasite.

Expressing:

Article Title: Hepatic-stellate-cell-targeted delivery of PU.1 decoy ODN by Apt-Tan attenuates liver fibrosis in mice
Article Snippet: .. For protein expression analysis, transfected cells were washed with cold PBS and lysed in cell lysis buffer (Promega) with protease inhibitor cocktail for 30 min on ice. ..

Transfection:

Article Title: Hepatic-stellate-cell-targeted delivery of PU.1 decoy ODN by Apt-Tan attenuates liver fibrosis in mice
Article Snippet: .. For protein expression analysis, transfected cells were washed with cold PBS and lysed in cell lysis buffer (Promega) with protease inhibitor cocktail for 30 min on ice. ..

Lysis:

Article Title: Hepatic-stellate-cell-targeted delivery of PU.1 decoy ODN by Apt-Tan attenuates liver fibrosis in mice
Article Snippet: .. For protein expression analysis, transfected cells were washed with cold PBS and lysed in cell lysis buffer (Promega) with protease inhibitor cocktail for 30 min on ice. ..

Article Title: Trispecific SEED antibodies engineered for neutrophil-mediated cell killing.
Article Snippet: .. Complete lysis was assessed in wells where 11 μL Lysis Solution (Promega) was added to the medium 45 min previously to the completion of the incubation. ..

Article Title: Device, method and composition for transfection of cells with nucleic acids
Article Snippet: .. Specifically, after being washed with Phosphate Buffered Saline (PBS), the cells were lysed with 110 μL/well of lysis solution (Promega, Italy) and subjected to freezing-thawing cycles to promote lysis. .. 20 μL of cell lysate was then mixed with 50 μL of Luciferase Assay reagent (Promega, Italy) to measure luciferase activity.

Protease Inhibitor:

Article Title: Hepatic-stellate-cell-targeted delivery of PU.1 decoy ODN by Apt-Tan attenuates liver fibrosis in mice
Article Snippet: .. For protein expression analysis, transfected cells were washed with cold PBS and lysed in cell lysis buffer (Promega) with protease inhibitor cocktail for 30 min on ice. ..

Article Title: Method for automated protein purification
Article Snippet: .. To the resuspended cell pellets, 10 ul Lysozyme (100×, 10 mg/ml), 1 μl rlysozyme, 1 μl Benzonase Nuclease, and 11 μl Promega Protease Inhibitor Cocktail G6521 (50×, EDTA-free) (Promega Corporation, Fitchburg, WI) were mixed well and incubated on ice for 5-10 minutes. ..

Incubation:

Article Title: Trispecific SEED antibodies engineered for neutrophil-mediated cell killing.
Article Snippet: .. Complete lysis was assessed in wells where 11 μL Lysis Solution (Promega) was added to the medium 45 min previously to the completion of the incubation. ..

Article Title: Method for automated protein purification
Article Snippet: .. To the resuspended cell pellets, 10 ul Lysozyme (100×, 10 mg/ml), 1 μl rlysozyme, 1 μl Benzonase Nuclease, and 11 μl Promega Protease Inhibitor Cocktail G6521 (50×, EDTA-free) (Promega Corporation, Fitchburg, WI) were mixed well and incubated on ice for 5-10 minutes. ..

Saline:

Article Title: Device, method and composition for transfection of cells with nucleic acids
Article Snippet: .. Specifically, after being washed with Phosphate Buffered Saline (PBS), the cells were lysed with 110 μL/well of lysis solution (Promega, Italy) and subjected to freezing-thawing cycles to promote lysis. .. 20 μL of cell lysate was then mixed with 50 μL of Luciferase Assay reagent (Promega, Italy) to measure luciferase activity.



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