Journal: STAR Protocols

Article Title: Assessment of neurodegeneration and neuronal loss in aged 5XFAD mice

doi: 10.1016/j.xpro.2021.100915

Figure Lengend Snippet:

Article Snippet: Xylazine , Selleck , Cat# S2516.

Techniques: Recombinant, Software, Microscopy, Fluorescence

Journal: Journal of Bone Oncology

Article Title: Optimization of a syngeneic murine model of bone metastasis

doi: 10.1016/j.jbo.2020.100298

Figure Lengend Snippet: List of materials used in this syngeneic model of bone metastasis.

Article Snippet: Xylazine hydrochloride (Vetranal™) , Sigma Aldrich , 46995-100MG.

Techniques: Plasmid Preparation, Microscopy

Journal: Nature Communications

Article Title: Specific pharmacological and G i/o protein responses of some native GPCRs in neurons

doi: 10.1038/s41467-024-46177-z

Figure Lengend Snippet: a Effect of the indicated α 2 AR agonists on the net BRET of the G i1 and G oA sensors in CGNs co-transfected with Gα i1 Nluc and Venus Gγ 2 or Gα oA Nluc and Venus Gγ 2 (amounts of cDNA as in Fig. ). b Effect of the indicated α 2 AR agonists on the net BRET of the G i1 and G oA sensors in HEK293 cells co-transfected with the mouse α 2A AR (10 ng, 20 ng or 50 ng), and Gβ 1, Venus Gγ 2 and Gα i1 Nluc or Gα oA Nluc as in Fig. . Saturating concentrations of brimonidine (10 μM), oxymetazoline (100 μM), xylazine (50 μM), clonidine (100 μM), tizanidine (100 μM) were used in ( a , b ). c Detection of α 2 AR in cell membranes of CGNs, mock-transfected HEK293 cells and HEK293 cells transfected with indicated amount of α 2 AR, by western blotting. Values are mean ± SEM normalized as fold of α 2 AR expression in CGNs from four independent experiments. d The pEC 50 of brimonidine in CGNs ( n = 3) and transfected HEK293 cells ( n = 4) with indicated amount of α 2 AR measured by G i1 or G oA sensor. e Percentage of change in BRET ratio between Gα Nluc and Venus Gγ 2 induced by brimonidine between Gα Nluc and Venus Gγ 2 in HEK293 cells ( n = 3) (α 2 AR: 15 ng/well per 96-well plate) or CGNs ( n = 4) for the indicated Gα i1 , Gα i2 , Gα i3 , Gα oA , Gα oB or Gα z sensors (amounts of cDNA as in Fig. ). Values are mean ± SEM from at least three biologically independent experiments each performed in triplicate or quadruplicate in ( a , b , d , e ). a , n = 4; b , n = 3; ( d , e ), CGNs, n = 3; HEK293 cells, n = 4. Data are analysed using one-way ANOVA with a Dunnett’s post-hoc multiple comparison test to determine significance (compared with brimonidine in a , b ). Data are analysed using unpaired t -test (two-tailed) in ( e ). **** p < 0.0001, *** p < 0.001, ** p < 0.01 and not significant (ns). The raw data and p -values are available in source data provided as a Source Data file.

Article Snippet: Win 55,212-2 (T4458), Bay 59-3074 (T3699), DAMGO (T4351), eletriptan HBr (T0216), pramipexole 2HCl monohydrate (T6951), brimonidine tartrate (T6422), tizanidine hydrochloride (T0290), oxymetazoline hydrochloride (T0252), xylazine (T7046), clonidine hydrochloride (T1247), and 1-oleoyl lysophosphatidic acid sodium (T21654) were purchased from TargetMol (Shanghai, China).

Techniques: Transfection, Western Blot, Expressing, Comparison, Two Tailed Test