Journal: Journal of molecular histology

Article Title: tsRNA-3043a intensifies apoptosis and senescence of ovarian granulosa cells to drive premature ovarian failure by targeting FLT1.

doi: 10.1007/s10735-024-10256-8

Figure Lengend Snippet: Fig. 10 Diagram of the hypothesis mechanism of tsRNA-3043a facilitates POF by inhibiting FLT1

Article Snippet: The sections were blocked with 5% BSA and incubated with the anti-FLT1 antibody (1:200, 13687-1-AP, Proteintech) overnight at 4°C.

Techniques:

Journal: Translational Vision Science & Technology

Article Title: Sema3A Antibody BI-X Prevents Cell Permeability and Cytoskeletal Collapse in HRMECs and Increases Tip Cell Density in Mouse Oxygen-Induced Retinopathy

doi: 10.1167/tvst.11.6.17

Figure Lengend Snippet: Diagrammatic representation of sprouting angiogenesis in the retina, adapted from Sapieha. Initially, tip cell filopodia are stimulated by proangiogenic factors, such as VEGF-A. Via a combination of pericyte detachment from the vascular basement membrane, matrix metalloproteinase-induced degradation of the basement membrane, and loosening of VE–cadherin junctions, tip cell filopodia migrate through the basement membrane and search for vascular guidance cues. Chemoattractive cues include VEGF-A, whereas chemorepulsive cues include Sema3A. , Subsequently, endothelial cells adjacent to the tip cells (i.e., “stalk” cells) proliferate in response to proangiogenic factors (e.g., VEGF-A) to elongate the neovessels. – Sema3A, semaphorin 3A; VE, vascular endothelial; VEGF-A, vascular endothelial growth factor-A.

Article Snippet: The HRMECs were treated overnight with either 100 ng/mL recombinant human (rh) VEGF-A (293-VE010; R&D Systems, Inc., Minneapolis, MN, USA) or 500 ng/mL rh-Sema3A (1250-S3; R&D Systems) with and without 1 µg/mL of BI-X or 1 µg/mL of anti-trinitrophenol (TNP; 1B7.11 [ATCC TIB-191]; American Type Culture Collection, Manassas, VA, USA).

Techniques: Membrane

Journal: Translational Vision Science & Technology

Article Title: Sema3A Antibody BI-X Prevents Cell Permeability and Cytoskeletal Collapse in HRMECs and Increases Tip Cell Density in Mouse Oxygen-Induced Retinopathy

doi: 10.1167/tvst.11.6.17

Figure Lengend Snippet: Effects of BI-X, Sema3A, and VEGF-A on HRMEC permeability in vitro. Data are presented as mean ± SEM (group size, eight wells/group) and represent one of two independent experiments with comparable results. Statistical analysis was performed by one-way analysis of variance with Dunnett's post hoc multiple comparisons test versus Sema3A (*** P < 0.001). HRMEC, human retinal microvascular endothelial cell; SEM, standard error of the mean; Sema3A, semaphorin 3A; TNP, trinitrophenol; VEGF-A, vascular endothelial growth factor-A.

Article Snippet: The HRMECs were treated overnight with either 100 ng/mL recombinant human (rh) VEGF-A (293-VE010; R&D Systems, Inc., Minneapolis, MN, USA) or 500 ng/mL rh-Sema3A (1250-S3; R&D Systems) with and without 1 µg/mL of BI-X or 1 µg/mL of anti-trinitrophenol (TNP; 1B7.11 [ATCC TIB-191]; American Type Culture Collection, Manassas, VA, USA).

Techniques: Permeability, In Vitro