Journal: Neuron

Article Title: PDGFRβ Cells Rapidly Relay Inflammatory Signal from the Circulatory System to Neurons via Chemokine CCL2.

doi: 10.1016/j.neuron.2018.08.030

Figure Lengend Snippet: Figure 6. CCL2 Promotes Excitatory Synaptic Transmission through CCR2 and PLC Signaling (A and B) The effect of CCL2 application on mEPSC frequency and amplitude in CA1 pyramidal neurons of Ccr2 KO mice, representative traces (A) and summary data (B) (13 cells from 3 mice; Wilcoxon matched-pairs signed rank test). (C and D) The effect of CCL2 application on firing responses to stepwise current injections in CA1 pyramidal neurons of Ccr2 KO mice, representative traces (C) and summary data (D) (12–13 cells from 2 mice; two-way ANOVA, Bonferroni post hoc test). (E–H) mEPSC recordings from Lenti-Ccr2 RNAi infected CA1 pyramidal neurons (E and F), or neighboring uninfected neurons (G and H) before and after CCL2 application, representative traces (E, G) and summary data (F, H) (8–10 cells from 5 mice per condition; Wilcoxon matched-pairs signed rank test). (I–L) mEPSC recordings before and after CCL2 application with 10 mM U73122 in aCSF (I and J) or with 5 mM BAPTA tetracesium in the internal cellular solution (K and L), representative traces (I, K) and summary data (J, L) (9–11 cells from 3 mice; Wilcoxon matched-pairs signed rank test). See also Figure S7.

Article Snippet: DH5a TIANGEN Biotech Cat# CB101 Lentiviruses Genechem, Shanghai, China N/A Chemicals, Peptides, and Recombinant Proteins Lipopolysaccharides (Escherichia coli, serotype O111:B4) Sigma Cat# L2630-25MG Poly(I:C) Tocris Cat# 4287 ODN-1668 InvivoGen Cat# tlrl-1668-5 DAPI Thermo Fisher Scientific Cat# D1306; RRID:AB_2629482 Recombinant Mouse CCL2/JE/MCP-1 R&D Systems Cat# 479-JE-050 BAPTA tetracesium salt Thermo Fisher Scientific Cat# B-1212 NBQX Tocris Cat# 1044 D-APV Tocris Cat# 0106 Gabazine Tocris Cat# 1262 U73122 Tocris Cat# 1268 Fast red Roche Cat# 11496549001 (Continued on next page) Neuron 100, 1–18.e1–e8, October 10, 2018 e1

Techniques: Transmission Assay, Infection

Journal: Molecular carcinogenesis

Article Title: UCP2 Upregulation Promotes PLCγ-1 Signaling During Skin Cell Transformation

doi: 10.1002/mc.22684

Figure Lengend Snippet: Knockdown of PLCγ-1 by the siRNA approach (A) suppressed anchorage-independent growth (B) of UCP2 overexpressed cells (N=3 per group). The PLCγ-1 inhibitor U73122 suppressed PLCγ-1 expression (C), the levels of its downstream targets: IP3 (D, N=3 per group) and DAG (E, N=3 per group), as well as 3D spheroid formation (F, N=6 per group) of UCP2 overexpressed cells. (G) Knockdown of PLCγ-1 by a second set of siRNA significantly decreased cell proliferation of UCP2 overexpressed cells. A Two-way ANOVA revealed significance effects. *, indicates significant differences (p<0.001) between control TPA and control DMSO group. #, indicates significant differences (p<0.001) between siRNA PLCγ-1 DMSO and control DMSO group. +, indicates significant differences (p<0.001) between siRNA PLCγ-1 TPA and control DMSO group. For Figure 6C–E, UCP2 cells were treated with TPA (5 nM) or vehicle control (DMSO) for 24 h. *, p<0.05 when compared with its own control group. #, p<0.05 when compared with the control/TPA group.

Article Snippet: Cells were treated with catalase (2000 units/ml, Sigma) to inhibit hydrogen peroxide levels, and PLCγ-1 was inhibited by 5 μM {"type":"entrez-nucleotide","attrs":{"text":"U73122","term_id":"4098075","term_text":"U73122"}} U73122 (Item No 70740, Cayman Chemicals).

Techniques: Expressing