Journal: Molecular Therapy

Article Title: Identification of Epigenetic Regulators of DUX4-fl for Targeted Therapy of Facioscapulohumeral Muscular Dystrophy

doi: 10.1016/j.ymthe.2018.04.019

Figure Lengend Snippet: Transcriptional Repression of Epigenetic Regulators by dCas9-KRAB Reduces Expression of DUX4-fl in FSHD Myocytes (A–D) Differentiated FSHD myocytes were subjected to four serial co-infections with combinations of lentiviral supernatants expressing either dCas9-KRAB or individual sgRNAs (g1–2 for each target gene) targeting BAZ1A (A), BRD2 (B), KDM4C (C), or SMARCA5 (D). Cells were harvested ∼72 hr later for analysis of gene expression by qRT-PCR (as in Figure 1 ). Data are plotted as the mean + SD value of three independent experiments, with relative mRNA expression for cells infected with dCas9-KRAB alone set to 1. *p < 0.05 and **p < 0.01 are from comparing sgRNAs to dCas9-KRAB alone. Refer to the , Figure S3 , and and for additional details.

Article Snippet: The pHAGE EF1-dCas9-KRAB (Addgene plasmid 50919) and pLKO.1-puro U6 sgRNA BfuAI stuffer lentiviral plasmids were developed by Rene Maehr and Scot Wolfe (Addgene plasmid 50920).

Techniques: Expressing, Gene Expression, Quantitative RT-PCR, Infection