streptavidin Search Results


streptavidin conjugated with phycoerythrin  (Agilent technologies)
99
Agilent technologies streptavidin conjugated with phycoerythrin
Streptavidin Conjugated With Phycoerythrin, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/streptavidin conjugated with phycoerythrin/product/Agilent technologies
Average 99 stars, based on 1 article reviews
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99/100 stars
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dynabeads myone streptavidin c1  (Thermo Fisher)
99
Thermo Fisher dynabeads myone streptavidin c1
(A) Design of bio-inspired probe biotin functionalized biguanide (BFB) based on the structure of metformin (Met). (B) Immunoblots for the phosphorylation of AMPK (Thr172) and ACC (Ser79) in extracts from KP-4 pancreatic cancer cells treated with 2.5 mM Met or BFB for 16 hours. β-ACTIN was used as loading control. (C) Representative quantification of cell viability and growth with corresponding EC 50 values of 3-day treatments with metformin (Met) or biotin functionalized biguanide (BFB) in KP-4 cells. Values represent the mean ± standard deviation of N=3. (D) Representative images of mitochondria and BFB localization in cells as in (B). Cells were treated with 1 mM of metformin (Met) or BFB for 16 hours and mitochondrial signal and BFB localization were analyzed by co-immunofluorescence using <t>Streptavidin</t> fluorophore conjugate and TOMM20 antibody, scale bar= 10 μm. Cells untreated (-) and treated with 1 mM Met were used as negative controls. (E) Colocalization between TOMM20 (TOMM20-568) and Streptavidin (Strep-488) fluorophores was analyzed for the BFB condition from (D) through job plot intensity profile. (F) Pull-down validation experiments with streptavidin beads alone (-), D-biotin (B), BFA and BFB using antibody followed by immunoblot against ATP5I in cells as in in extracts from HEK-293T embryonic kidney cells. The whole cell lysate (WCL) was added as control. (G) Binding interactions studies of BFB with recombinant purified ATP5I (rATP5I) using Surface Plasmons of Resonance (SPR). Representative sensorgrams show affinity kinetics of BFB and rATP5I. BFB was exposed onto streptavidin immobilized sensor chip and several concentrations of rATP5I were added until saturation of the signal. The assay was performed in a running buffer (125 mM NaCl, 5 mM DTT, PBS pH 7.4) at 25°C. RU: Resonance Units. (H) Binding affinity curve obtained from each steady state from (F). K D refers to the dissociation equilibrium constant and Rmax represent the theoretical maximum response.
Dynabeads Myone Streptavidin C1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dynabeads myone streptavidin c1/product/Thermo Fisher
Average 99 stars, based on 1 article reviews
dynabeads myone streptavidin c1 - by Bioz Stars, 2026-04
99/100 stars
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streptavidin horseradish peroxidase  (SouthernBiotech)
96
SouthernBiotech streptavidin horseradish peroxidase
(A) Design of bio-inspired probe biotin functionalized biguanide (BFB) based on the structure of metformin (Met). (B) Immunoblots for the phosphorylation of AMPK (Thr172) and ACC (Ser79) in extracts from KP-4 pancreatic cancer cells treated with 2.5 mM Met or BFB for 16 hours. β-ACTIN was used as loading control. (C) Representative quantification of cell viability and growth with corresponding EC 50 values of 3-day treatments with metformin (Met) or biotin functionalized biguanide (BFB) in KP-4 cells. Values represent the mean ± standard deviation of N=3. (D) Representative images of mitochondria and BFB localization in cells as in (B). Cells were treated with 1 mM of metformin (Met) or BFB for 16 hours and mitochondrial signal and BFB localization were analyzed by co-immunofluorescence using <t>Streptavidin</t> fluorophore conjugate and TOMM20 antibody, scale bar= 10 μm. Cells untreated (-) and treated with 1 mM Met were used as negative controls. (E) Colocalization between TOMM20 (TOMM20-568) and Streptavidin (Strep-488) fluorophores was analyzed for the BFB condition from (D) through job plot intensity profile. (F) Pull-down validation experiments with streptavidin beads alone (-), D-biotin (B), BFA and BFB using antibody followed by immunoblot against ATP5I in cells as in in extracts from HEK-293T embryonic kidney cells. The whole cell lysate (WCL) was added as control. (G) Binding interactions studies of BFB with recombinant purified ATP5I (rATP5I) using Surface Plasmons of Resonance (SPR). Representative sensorgrams show affinity kinetics of BFB and rATP5I. BFB was exposed onto streptavidin immobilized sensor chip and several concentrations of rATP5I were added until saturation of the signal. The assay was performed in a running buffer (125 mM NaCl, 5 mM DTT, PBS pH 7.4) at 25°C. RU: Resonance Units. (H) Binding affinity curve obtained from each steady state from (F). K D refers to the dissociation equilibrium constant and Rmax represent the theoretical maximum response.
Streptavidin Horseradish Peroxidase, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/streptavidin horseradish peroxidase/product/SouthernBiotech
Average 96 stars, based on 1 article reviews
streptavidin horseradish peroxidase - by Bioz Stars, 2026-04
96/100 stars
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fitc streptavidin  (Vector Laboratories)
99
Vector Laboratories fitc streptavidin
(A) Design of bio-inspired probe biotin functionalized biguanide (BFB) based on the structure of metformin (Met). (B) Immunoblots for the phosphorylation of AMPK (Thr172) and ACC (Ser79) in extracts from KP-4 pancreatic cancer cells treated with 2.5 mM Met or BFB for 16 hours. β-ACTIN was used as loading control. (C) Representative quantification of cell viability and growth with corresponding EC 50 values of 3-day treatments with metformin (Met) or biotin functionalized biguanide (BFB) in KP-4 cells. Values represent the mean ± standard deviation of N=3. (D) Representative images of mitochondria and BFB localization in cells as in (B). Cells were treated with 1 mM of metformin (Met) or BFB for 16 hours and mitochondrial signal and BFB localization were analyzed by co-immunofluorescence using <t>Streptavidin</t> fluorophore conjugate and TOMM20 antibody, scale bar= 10 μm. Cells untreated (-) and treated with 1 mM Met were used as negative controls. (E) Colocalization between TOMM20 (TOMM20-568) and Streptavidin (Strep-488) fluorophores was analyzed for the BFB condition from (D) through job plot intensity profile. (F) Pull-down validation experiments with streptavidin beads alone (-), D-biotin (B), BFA and BFB using antibody followed by immunoblot against ATP5I in cells as in in extracts from HEK-293T embryonic kidney cells. The whole cell lysate (WCL) was added as control. (G) Binding interactions studies of BFB with recombinant purified ATP5I (rATP5I) using Surface Plasmons of Resonance (SPR). Representative sensorgrams show affinity kinetics of BFB and rATP5I. BFB was exposed onto streptavidin immobilized sensor chip and several concentrations of rATP5I were added until saturation of the signal. The assay was performed in a running buffer (125 mM NaCl, 5 mM DTT, PBS pH 7.4) at 25°C. RU: Resonance Units. (H) Binding affinity curve obtained from each steady state from (F). K D refers to the dissociation equilibrium constant and Rmax represent the theoretical maximum response.
Fitc Streptavidin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fitc streptavidin/product/Vector Laboratories
Average 99 stars, based on 1 article reviews
fitc streptavidin - by Bioz Stars, 2026-04
99/100 stars
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streptavidin  (Jackson Immuno)
96
Jackson Immuno streptavidin
(A) Design of bio-inspired probe biotin functionalized biguanide (BFB) based on the structure of metformin (Met). (B) Immunoblots for the phosphorylation of AMPK (Thr172) and ACC (Ser79) in extracts from KP-4 pancreatic cancer cells treated with 2.5 mM Met or BFB for 16 hours. β-ACTIN was used as loading control. (C) Representative quantification of cell viability and growth with corresponding EC 50 values of 3-day treatments with metformin (Met) or biotin functionalized biguanide (BFB) in KP-4 cells. Values represent the mean ± standard deviation of N=3. (D) Representative images of mitochondria and BFB localization in cells as in (B). Cells were treated with 1 mM of metformin (Met) or BFB for 16 hours and mitochondrial signal and BFB localization were analyzed by co-immunofluorescence using <t>Streptavidin</t> fluorophore conjugate and TOMM20 antibody, scale bar= 10 μm. Cells untreated (-) and treated with 1 mM Met were used as negative controls. (E) Colocalization between TOMM20 (TOMM20-568) and Streptavidin (Strep-488) fluorophores was analyzed for the BFB condition from (D) through job plot intensity profile. (F) Pull-down validation experiments with streptavidin beads alone (-), D-biotin (B), BFA and BFB using antibody followed by immunoblot against ATP5I in cells as in in extracts from HEK-293T embryonic kidney cells. The whole cell lysate (WCL) was added as control. (G) Binding interactions studies of BFB with recombinant purified ATP5I (rATP5I) using Surface Plasmons of Resonance (SPR). Representative sensorgrams show affinity kinetics of BFB and rATP5I. BFB was exposed onto streptavidin immobilized sensor chip and several concentrations of rATP5I were added until saturation of the signal. The assay was performed in a running buffer (125 mM NaCl, 5 mM DTT, PBS pH 7.4) at 25°C. RU: Resonance Units. (H) Binding affinity curve obtained from each steady state from (F). K D refers to the dissociation equilibrium constant and Rmax represent the theoretical maximum response.
Streptavidin, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/streptavidin/product/Jackson Immuno
Average 96 stars, based on 1 article reviews
streptavidin - by Bioz Stars, 2026-04
96/100 stars
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cy3 conjugated streptavidin  (Jackson Immuno)
96
Jackson Immuno cy3 conjugated streptavidin
(A) Design of bio-inspired probe biotin functionalized biguanide (BFB) based on the structure of metformin (Met). (B) Immunoblots for the phosphorylation of AMPK (Thr172) and ACC (Ser79) in extracts from KP-4 pancreatic cancer cells treated with 2.5 mM Met or BFB for 16 hours. β-ACTIN was used as loading control. (C) Representative quantification of cell viability and growth with corresponding EC 50 values of 3-day treatments with metformin (Met) or biotin functionalized biguanide (BFB) in KP-4 cells. Values represent the mean ± standard deviation of N=3. (D) Representative images of mitochondria and BFB localization in cells as in (B). Cells were treated with 1 mM of metformin (Met) or BFB for 16 hours and mitochondrial signal and BFB localization were analyzed by co-immunofluorescence using <t>Streptavidin</t> fluorophore conjugate and TOMM20 antibody, scale bar= 10 μm. Cells untreated (-) and treated with 1 mM Met were used as negative controls. (E) Colocalization between TOMM20 (TOMM20-568) and Streptavidin (Strep-488) fluorophores was analyzed for the BFB condition from (D) through job plot intensity profile. (F) Pull-down validation experiments with streptavidin beads alone (-), D-biotin (B), BFA and BFB using antibody followed by immunoblot against ATP5I in cells as in in extracts from HEK-293T embryonic kidney cells. The whole cell lysate (WCL) was added as control. (G) Binding interactions studies of BFB with recombinant purified ATP5I (rATP5I) using Surface Plasmons of Resonance (SPR). Representative sensorgrams show affinity kinetics of BFB and rATP5I. BFB was exposed onto streptavidin immobilized sensor chip and several concentrations of rATP5I were added until saturation of the signal. The assay was performed in a running buffer (125 mM NaCl, 5 mM DTT, PBS pH 7.4) at 25°C. RU: Resonance Units. (H) Binding affinity curve obtained from each steady state from (F). K D refers to the dissociation equilibrium constant and Rmax represent the theoretical maximum response.
Cy3 Conjugated Streptavidin, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cy3 conjugated streptavidin/product/Jackson Immuno
Average 96 stars, based on 1 article reviews
cy3 conjugated streptavidin - by Bioz Stars, 2026-04
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δ clone sba 1 southern biotechnology associates birmingham al  (SouthernBiotech)
93
SouthernBiotech δ clone sba 1 southern biotechnology associates birmingham al
(A) Design of bio-inspired probe biotin functionalized biguanide (BFB) based on the structure of metformin (Met). (B) Immunoblots for the phosphorylation of AMPK (Thr172) and ACC (Ser79) in extracts from KP-4 pancreatic cancer cells treated with 2.5 mM Met or BFB for 16 hours. β-ACTIN was used as loading control. (C) Representative quantification of cell viability and growth with corresponding EC 50 values of 3-day treatments with metformin (Met) or biotin functionalized biguanide (BFB) in KP-4 cells. Values represent the mean ± standard deviation of N=3. (D) Representative images of mitochondria and BFB localization in cells as in (B). Cells were treated with 1 mM of metformin (Met) or BFB for 16 hours and mitochondrial signal and BFB localization were analyzed by co-immunofluorescence using <t>Streptavidin</t> fluorophore conjugate and TOMM20 antibody, scale bar= 10 μm. Cells untreated (-) and treated with 1 mM Met were used as negative controls. (E) Colocalization between TOMM20 (TOMM20-568) and Streptavidin (Strep-488) fluorophores was analyzed for the BFB condition from (D) through job plot intensity profile. (F) Pull-down validation experiments with streptavidin beads alone (-), D-biotin (B), BFA and BFB using antibody followed by immunoblot against ATP5I in cells as in in extracts from HEK-293T embryonic kidney cells. The whole cell lysate (WCL) was added as control. (G) Binding interactions studies of BFB with recombinant purified ATP5I (rATP5I) using Surface Plasmons of Resonance (SPR). Representative sensorgrams show affinity kinetics of BFB and rATP5I. BFB was exposed onto streptavidin immobilized sensor chip and several concentrations of rATP5I were added until saturation of the signal. The assay was performed in a running buffer (125 mM NaCl, 5 mM DTT, PBS pH 7.4) at 25°C. RU: Resonance Units. (H) Binding affinity curve obtained from each steady state from (F). K D refers to the dissociation equilibrium constant and Rmax represent the theoretical maximum response.
δ Clone Sba 1 Southern Biotechnology Associates Birmingham Al, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/δ clone sba 1 southern biotechnology associates birmingham al/product/SouthernBiotech
Average 93 stars, based on 1 article reviews
δ clone sba 1 southern biotechnology associates birmingham al - by Bioz Stars, 2026-04
93/100 stars
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biotinylated anti rat igg h l  (Vector Laboratories)
93
Vector Laboratories biotinylated anti rat igg h l
(A) Design of bio-inspired probe biotin functionalized biguanide (BFB) based on the structure of metformin (Met). (B) Immunoblots for the phosphorylation of AMPK (Thr172) and ACC (Ser79) in extracts from KP-4 pancreatic cancer cells treated with 2.5 mM Met or BFB for 16 hours. β-ACTIN was used as loading control. (C) Representative quantification of cell viability and growth with corresponding EC 50 values of 3-day treatments with metformin (Met) or biotin functionalized biguanide (BFB) in KP-4 cells. Values represent the mean ± standard deviation of N=3. (D) Representative images of mitochondria and BFB localization in cells as in (B). Cells were treated with 1 mM of metformin (Met) or BFB for 16 hours and mitochondrial signal and BFB localization were analyzed by co-immunofluorescence using <t>Streptavidin</t> fluorophore conjugate and TOMM20 antibody, scale bar= 10 μm. Cells untreated (-) and treated with 1 mM Met were used as negative controls. (E) Colocalization between TOMM20 (TOMM20-568) and Streptavidin (Strep-488) fluorophores was analyzed for the BFB condition from (D) through job plot intensity profile. (F) Pull-down validation experiments with streptavidin beads alone (-), D-biotin (B), BFA and BFB using antibody followed by immunoblot against ATP5I in cells as in in extracts from HEK-293T embryonic kidney cells. The whole cell lysate (WCL) was added as control. (G) Binding interactions studies of BFB with recombinant purified ATP5I (rATP5I) using Surface Plasmons of Resonance (SPR). Representative sensorgrams show affinity kinetics of BFB and rATP5I. BFB was exposed onto streptavidin immobilized sensor chip and several concentrations of rATP5I were added until saturation of the signal. The assay was performed in a running buffer (125 mM NaCl, 5 mM DTT, PBS pH 7.4) at 25°C. RU: Resonance Units. (H) Binding affinity curve obtained from each steady state from (F). K D refers to the dissociation equilibrium constant and Rmax represent the theoretical maximum response.
Biotinylated Anti Rat Igg H L, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biotinylated anti rat igg h l/product/Vector Laboratories
Average 93 stars, based on 1 article reviews
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streptavidin microbeads  (Miltenyi Biotec)
96
Miltenyi Biotec streptavidin microbeads
(A) Design of bio-inspired probe biotin functionalized biguanide (BFB) based on the structure of metformin (Met). (B) Immunoblots for the phosphorylation of AMPK (Thr172) and ACC (Ser79) in extracts from KP-4 pancreatic cancer cells treated with 2.5 mM Met or BFB for 16 hours. β-ACTIN was used as loading control. (C) Representative quantification of cell viability and growth with corresponding EC 50 values of 3-day treatments with metformin (Met) or biotin functionalized biguanide (BFB) in KP-4 cells. Values represent the mean ± standard deviation of N=3. (D) Representative images of mitochondria and BFB localization in cells as in (B). Cells were treated with 1 mM of metformin (Met) or BFB for 16 hours and mitochondrial signal and BFB localization were analyzed by co-immunofluorescence using <t>Streptavidin</t> fluorophore conjugate and TOMM20 antibody, scale bar= 10 μm. Cells untreated (-) and treated with 1 mM Met were used as negative controls. (E) Colocalization between TOMM20 (TOMM20-568) and Streptavidin (Strep-488) fluorophores was analyzed for the BFB condition from (D) through job plot intensity profile. (F) Pull-down validation experiments with streptavidin beads alone (-), D-biotin (B), BFA and BFB using antibody followed by immunoblot against ATP5I in cells as in in extracts from HEK-293T embryonic kidney cells. The whole cell lysate (WCL) was added as control. (G) Binding interactions studies of BFB with recombinant purified ATP5I (rATP5I) using Surface Plasmons of Resonance (SPR). Representative sensorgrams show affinity kinetics of BFB and rATP5I. BFB was exposed onto streptavidin immobilized sensor chip and several concentrations of rATP5I were added until saturation of the signal. The assay was performed in a running buffer (125 mM NaCl, 5 mM DTT, PBS pH 7.4) at 25°C. RU: Resonance Units. (H) Binding affinity curve obtained from each steady state from (F). K D refers to the dissociation equilibrium constant and Rmax represent the theoretical maximum response.
Streptavidin Microbeads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/streptavidin microbeads/product/Miltenyi Biotec
Average 96 stars, based on 1 article reviews
streptavidin microbeads - by Bioz Stars, 2026-04
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irdye 800cw streptavidin  (LI-COR)
96
LI-COR irdye 800cw streptavidin
(A) Design of bio-inspired probe biotin functionalized biguanide (BFB) based on the structure of metformin (Met). (B) Immunoblots for the phosphorylation of AMPK (Thr172) and ACC (Ser79) in extracts from KP-4 pancreatic cancer cells treated with 2.5 mM Met or BFB for 16 hours. β-ACTIN was used as loading control. (C) Representative quantification of cell viability and growth with corresponding EC 50 values of 3-day treatments with metformin (Met) or biotin functionalized biguanide (BFB) in KP-4 cells. Values represent the mean ± standard deviation of N=3. (D) Representative images of mitochondria and BFB localization in cells as in (B). Cells were treated with 1 mM of metformin (Met) or BFB for 16 hours and mitochondrial signal and BFB localization were analyzed by co-immunofluorescence using <t>Streptavidin</t> fluorophore conjugate and TOMM20 antibody, scale bar= 10 μm. Cells untreated (-) and treated with 1 mM Met were used as negative controls. (E) Colocalization between TOMM20 (TOMM20-568) and Streptavidin (Strep-488) fluorophores was analyzed for the BFB condition from (D) through job plot intensity profile. (F) Pull-down validation experiments with streptavidin beads alone (-), D-biotin (B), BFA and BFB using antibody followed by immunoblot against ATP5I in cells as in in extracts from HEK-293T embryonic kidney cells. The whole cell lysate (WCL) was added as control. (G) Binding interactions studies of BFB with recombinant purified ATP5I (rATP5I) using Surface Plasmons of Resonance (SPR). Representative sensorgrams show affinity kinetics of BFB and rATP5I. BFB was exposed onto streptavidin immobilized sensor chip and several concentrations of rATP5I were added until saturation of the signal. The assay was performed in a running buffer (125 mM NaCl, 5 mM DTT, PBS pH 7.4) at 25°C. RU: Resonance Units. (H) Binding affinity curve obtained from each steady state from (F). K D refers to the dissociation equilibrium constant and Rmax represent the theoretical maximum response.
Irdye 800cw Streptavidin, supplied by LI-COR, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/irdye 800cw streptavidin/product/LI-COR
Average 96 stars, based on 1 article reviews
irdye 800cw streptavidin - by Bioz Stars, 2026-04
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streptavidin horseradish peroxidase conjugate  (GE Healthcare)
94
GE Healthcare streptavidin horseradish peroxidase conjugate
(A) Design of bio-inspired probe biotin functionalized biguanide (BFB) based on the structure of metformin (Met). (B) Immunoblots for the phosphorylation of AMPK (Thr172) and ACC (Ser79) in extracts from KP-4 pancreatic cancer cells treated with 2.5 mM Met or BFB for 16 hours. β-ACTIN was used as loading control. (C) Representative quantification of cell viability and growth with corresponding EC 50 values of 3-day treatments with metformin (Met) or biotin functionalized biguanide (BFB) in KP-4 cells. Values represent the mean ± standard deviation of N=3. (D) Representative images of mitochondria and BFB localization in cells as in (B). Cells were treated with 1 mM of metformin (Met) or BFB for 16 hours and mitochondrial signal and BFB localization were analyzed by co-immunofluorescence using <t>Streptavidin</t> fluorophore conjugate and TOMM20 antibody, scale bar= 10 μm. Cells untreated (-) and treated with 1 mM Met were used as negative controls. (E) Colocalization between TOMM20 (TOMM20-568) and Streptavidin (Strep-488) fluorophores was analyzed for the BFB condition from (D) through job plot intensity profile. (F) Pull-down validation experiments with streptavidin beads alone (-), D-biotin (B), BFA and BFB using antibody followed by immunoblot against ATP5I in cells as in in extracts from HEK-293T embryonic kidney cells. The whole cell lysate (WCL) was added as control. (G) Binding interactions studies of BFB with recombinant purified ATP5I (rATP5I) using Surface Plasmons of Resonance (SPR). Representative sensorgrams show affinity kinetics of BFB and rATP5I. BFB was exposed onto streptavidin immobilized sensor chip and several concentrations of rATP5I were added until saturation of the signal. The assay was performed in a running buffer (125 mM NaCl, 5 mM DTT, PBS pH 7.4) at 25°C. RU: Resonance Units. (H) Binding affinity curve obtained from each steady state from (F). K D refers to the dissociation equilibrium constant and Rmax represent the theoretical maximum response.
Streptavidin Horseradish Peroxidase Conjugate, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/streptavidin horseradish peroxidase conjugate/product/GE Healthcare
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streptavidin magnetic beads smb  (New England Biolabs)
96
New England Biolabs streptavidin magnetic beads smb
(A) Design of bio-inspired probe biotin functionalized biguanide (BFB) based on the structure of metformin (Met). (B) Immunoblots for the phosphorylation of AMPK (Thr172) and ACC (Ser79) in extracts from KP-4 pancreatic cancer cells treated with 2.5 mM Met or BFB for 16 hours. β-ACTIN was used as loading control. (C) Representative quantification of cell viability and growth with corresponding EC 50 values of 3-day treatments with metformin (Met) or biotin functionalized biguanide (BFB) in KP-4 cells. Values represent the mean ± standard deviation of N=3. (D) Representative images of mitochondria and BFB localization in cells as in (B). Cells were treated with 1 mM of metformin (Met) or BFB for 16 hours and mitochondrial signal and BFB localization were analyzed by co-immunofluorescence using <t>Streptavidin</t> fluorophore conjugate and TOMM20 antibody, scale bar= 10 μm. Cells untreated (-) and treated with 1 mM Met were used as negative controls. (E) Colocalization between TOMM20 (TOMM20-568) and Streptavidin (Strep-488) fluorophores was analyzed for the BFB condition from (D) through job plot intensity profile. (F) Pull-down validation experiments with streptavidin beads alone (-), D-biotin (B), BFA and BFB using antibody followed by immunoblot against ATP5I in cells as in in extracts from HEK-293T embryonic kidney cells. The whole cell lysate (WCL) was added as control. (G) Binding interactions studies of BFB with recombinant purified ATP5I (rATP5I) using Surface Plasmons of Resonance (SPR). Representative sensorgrams show affinity kinetics of BFB and rATP5I. BFB was exposed onto streptavidin immobilized sensor chip and several concentrations of rATP5I were added until saturation of the signal. The assay was performed in a running buffer (125 mM NaCl, 5 mM DTT, PBS pH 7.4) at 25°C. RU: Resonance Units. (H) Binding affinity curve obtained from each steady state from (F). K D refers to the dissociation equilibrium constant and Rmax represent the theoretical maximum response.
Streptavidin Magnetic Beads Smb, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A) Design of bio-inspired probe biotin functionalized biguanide (BFB) based on the structure of metformin (Met). (B) Immunoblots for the phosphorylation of AMPK (Thr172) and ACC (Ser79) in extracts from KP-4 pancreatic cancer cells treated with 2.5 mM Met or BFB for 16 hours. β-ACTIN was used as loading control. (C) Representative quantification of cell viability and growth with corresponding EC 50 values of 3-day treatments with metformin (Met) or biotin functionalized biguanide (BFB) in KP-4 cells. Values represent the mean ± standard deviation of N=3. (D) Representative images of mitochondria and BFB localization in cells as in (B). Cells were treated with 1 mM of metformin (Met) or BFB for 16 hours and mitochondrial signal and BFB localization were analyzed by co-immunofluorescence using Streptavidin fluorophore conjugate and TOMM20 antibody, scale bar= 10 μm. Cells untreated (-) and treated with 1 mM Met were used as negative controls. (E) Colocalization between TOMM20 (TOMM20-568) and Streptavidin (Strep-488) fluorophores was analyzed for the BFB condition from (D) through job plot intensity profile. (F) Pull-down validation experiments with streptavidin beads alone (-), D-biotin (B), BFA and BFB using antibody followed by immunoblot against ATP5I in cells as in in extracts from HEK-293T embryonic kidney cells. The whole cell lysate (WCL) was added as control. (G) Binding interactions studies of BFB with recombinant purified ATP5I (rATP5I) using Surface Plasmons of Resonance (SPR). Representative sensorgrams show affinity kinetics of BFB and rATP5I. BFB was exposed onto streptavidin immobilized sensor chip and several concentrations of rATP5I were added until saturation of the signal. The assay was performed in a running buffer (125 mM NaCl, 5 mM DTT, PBS pH 7.4) at 25°C. RU: Resonance Units. (H) Binding affinity curve obtained from each steady state from (F). K D refers to the dissociation equilibrium constant and Rmax represent the theoretical maximum response.

Journal: bioRxiv

Article Title: The Role of ATP Synthase Subunit e (ATP5I) in Mediating the Metabolic and Antiproliferative Effects of Biguanides

doi: 10.1101/2024.09.20.614047

Figure Lengend Snippet: (A) Design of bio-inspired probe biotin functionalized biguanide (BFB) based on the structure of metformin (Met). (B) Immunoblots for the phosphorylation of AMPK (Thr172) and ACC (Ser79) in extracts from KP-4 pancreatic cancer cells treated with 2.5 mM Met or BFB for 16 hours. β-ACTIN was used as loading control. (C) Representative quantification of cell viability and growth with corresponding EC 50 values of 3-day treatments with metformin (Met) or biotin functionalized biguanide (BFB) in KP-4 cells. Values represent the mean ± standard deviation of N=3. (D) Representative images of mitochondria and BFB localization in cells as in (B). Cells were treated with 1 mM of metformin (Met) or BFB for 16 hours and mitochondrial signal and BFB localization were analyzed by co-immunofluorescence using Streptavidin fluorophore conjugate and TOMM20 antibody, scale bar= 10 μm. Cells untreated (-) and treated with 1 mM Met were used as negative controls. (E) Colocalization between TOMM20 (TOMM20-568) and Streptavidin (Strep-488) fluorophores was analyzed for the BFB condition from (D) through job plot intensity profile. (F) Pull-down validation experiments with streptavidin beads alone (-), D-biotin (B), BFA and BFB using antibody followed by immunoblot against ATP5I in cells as in in extracts from HEK-293T embryonic kidney cells. The whole cell lysate (WCL) was added as control. (G) Binding interactions studies of BFB with recombinant purified ATP5I (rATP5I) using Surface Plasmons of Resonance (SPR). Representative sensorgrams show affinity kinetics of BFB and rATP5I. BFB was exposed onto streptavidin immobilized sensor chip and several concentrations of rATP5I were added until saturation of the signal. The assay was performed in a running buffer (125 mM NaCl, 5 mM DTT, PBS pH 7.4) at 25°C. RU: Resonance Units. (H) Binding affinity curve obtained from each steady state from (F). K D refers to the dissociation equilibrium constant and Rmax represent the theoretical maximum response.

Article Snippet: In parallel, Dynabeads MyOne streptavidin C1 (65001, Thermo Fisher Scientific) were washed and resuspended at least three times in lauryl maltoside (LM) buffer [1% lauryl maltoside, cOmplete-EDTA free Protease Inhibitor Cocktail (Roche), PhosSTOP (Roche), PBS pH 7.8].

Techniques: Western Blot, Phospho-proteomics, Control, Standard Deviation, Immunofluorescence, Biomarker Discovery, Binding Assay, Recombinant, Purification