Journal: The Journal of Biological Chemistry

Article Title: Disease-associated mutations in a bifunctional aminoacyl-tRNA synthetase gene elicit the integrated stress response

doi: 10.1016/j.jbc.2021.101203

Figure Lengend Snippet: MSC association and protein integrity of EPRS-FLAG protein overexpressed in HEK293 T cells. A , doxycycline-inducible EPRS knockdown in HEK293T cells using shEPRS ( left ) or a nonspecific shRNA ( right ). B , immunoprecipitation experiments with FLAG antibody in HEK293T cells with endogenous EPRS knockdown and WT and mutant EPRS-FLAG overexpression. EPRS ( top ) and MSC scaffold proteins AIMP2 ( middle ) and AIMP3 ( bottom ) were visualized by immunoblotting using the indicated antibodies.

Article Snippet: Beads were washed three times with 0.02% PBS-T and boiled in SDS-PAGE loading buffer, followed by immunoblotting with the following antibodies: EPRS (Novus Biologicals NBP1-84929), AIMP2 (Thermo Scientific PA5-31306), AIMP3 (Invitrogen PA5-28283), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Bio-Rad).

Techniques: Knockdown, shRNA, Immunoprecipitation, Mutagenesis, Over Expression, Western Blot

Journal: The Journal of Biological Chemistry

Article Title: Disease-associated mutations in a bifunctional aminoacyl-tRNA synthetase gene elicit the integrated stress response

doi: 10.1016/j.jbc.2021.101203

Figure Lengend Snippet: Relative mRNA levels of EPRS 1 and ISR genes in WT and patient fibroblasts in response to ER stress. WT EPRS and patient-derived (MUT EPRS) fibroblast cells were cultured to about 70% confluency and treated with 1 μM thapsigargin (Tg) to induce ER stress or no stress treatment (NT) for 6 h. A , RNA was prepared from the cells and relative levels of EPRS 1 , ATF4 , and CHOP mRNAs were measured by qRT-PCR. The black and gray data points are from two experiments (indicated by black or gray dots ), each with three technical replicates. B , protein lysates were prepared from the cells and separated by SDS-PAGE, followed by immunoblotting to detect the indicated proteins. MW markers are shown in kDa. Results are representative of two independent experiments.

Article Snippet: Beads were washed three times with 0.02% PBS-T and boiled in SDS-PAGE loading buffer, followed by immunoblotting with the following antibodies: EPRS (Novus Biologicals NBP1-84929), AIMP2 (Thermo Scientific PA5-31306), AIMP3 (Invitrogen PA5-28283), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Bio-Rad).

Techniques: Derivative Assay, Cell Culture, Quantitative RT-PCR, SDS Page, Western Blot

Journal: Disease Markers

Article Title: Serum Survivin Levels and Outcome of Chemotherapy in Patients with Malignant Mesothelioma

doi: 10.1155/2015/316739

Figure Lengend Snippet: Survivin levels before chemotherapy and change in survivin levels after chemotherapy and MM treatment outcome.

Article Snippet: Serum survivin levels were determined using a human survivin enzyme-linked immunosorbent assay (ELISA) kit according to the manufacturer's instructions (Boster Biological Technology, Fremont, CA, USA) blinded regarding the study endpoints.

Techniques:

Journal: Disease Markers

Article Title: Serum Survivin Levels and Outcome of Chemotherapy in Patients with Malignant Mesothelioma

doi: 10.1155/2015/316739

Figure Lengend Snippet: Change in survivin levels after chemotherapy and response rate.

Article Snippet: Serum survivin levels were determined using a human survivin enzyme-linked immunosorbent assay (ELISA) kit according to the manufacturer's instructions (Boster Biological Technology, Fremont, CA, USA) blinded regarding the study endpoints.

Techniques:

Journal: Disease Markers

Article Title: Serum Survivin Levels and Outcome of Chemotherapy in Patients with Malignant Mesothelioma

doi: 10.1155/2015/316739

Figure Lengend Snippet: Receiver operating characteristic curve for the change in survivin levels predicting the response rate in malignant mesothelioma patients.

Article Snippet: Serum survivin levels were determined using a human survivin enzyme-linked immunosorbent assay (ELISA) kit according to the manufacturer's instructions (Boster Biological Technology, Fremont, CA, USA) blinded regarding the study endpoints.

Techniques:

Journal: Disease Markers

Article Title: Serum Survivin Levels and Outcome of Chemotherapy in Patients with Malignant Mesothelioma

doi: 10.1155/2015/316739

Figure Lengend Snippet: Change in serum survivin levels after chemotherapy is associated with (a) progression-free survival and (b) overall survival of malignant mesothelioma patients.

Article Snippet: Serum survivin levels were determined using a human survivin enzyme-linked immunosorbent assay (ELISA) kit according to the manufacturer's instructions (Boster Biological Technology, Fremont, CA, USA) blinded regarding the study endpoints.

Techniques: