rnase v1 Search Results


rnase v1  (Amersham Pharmacia Biotech Ltd)
90
Amersham Pharmacia Biotech Ltd rnase v1
Analysis of the complex formed between HTNV N protein and deletion RNAs by UV cross-linking analysis. The concentration of N protein used in each binding reaction was 3.5 × 10−6 M. Reaction mixtures were assembled in 100 mM NaCl with 5 mM MgCl2 in addition to standard reaction components as described in Material and Methods. Binding reactions were separated by sodium dodecyl sulfate–12% polyacrylamide gel electrophoresis, and unbound RNA was digested by adding 1 U of <t>RNase</t> <t>V1.</t> Signals were imaged with the Molecular Dynamics Storm PhosphorImager and quantified using ImageQuaNT version 4.2 software (Molecular Dynamics). The RNAs used to form the complexes are HTNV S-segment vRNA (lane 1), ORF RNA (lane 2), minipan RNA (lane 3), and Δ12 RNA (lane 4).
Rnase V1, supplied by Amersham Pharmacia Biotech Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rnase v1/product/Amersham Pharmacia Biotech Ltd
Average 90 stars, based on 1 article reviews
rnase v1 - by Bioz Stars, 2026-04
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rnase v1  (Boehringer Mannheim)
90
Boehringer Mannheim rnase v1
Analysis of the complex formed between HTNV N protein and deletion RNAs by UV cross-linking analysis. The concentration of N protein used in each binding reaction was 3.5 × 10−6 M. Reaction mixtures were assembled in 100 mM NaCl with 5 mM MgCl2 in addition to standard reaction components as described in Material and Methods. Binding reactions were separated by sodium dodecyl sulfate–12% polyacrylamide gel electrophoresis, and unbound RNA was digested by adding 1 U of <t>RNase</t> <t>V1.</t> Signals were imaged with the Molecular Dynamics Storm PhosphorImager and quantified using ImageQuaNT version 4.2 software (Molecular Dynamics). The RNAs used to form the complexes are HTNV S-segment vRNA (lane 1), ORF RNA (lane 2), minipan RNA (lane 3), and Δ12 RNA (lane 4).
Rnase V1, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rnase v1/product/Boehringer Mannheim
Average 90 stars, based on 1 article reviews
rnase v1 - by Bioz Stars, 2026-04
90/100 stars
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rnase v1  (Amano Inc)
90
Amano Inc rnase v1
Analysis of the complex formed between HTNV N protein and deletion RNAs by UV cross-linking analysis. The concentration of N protein used in each binding reaction was 3.5 × 10−6 M. Reaction mixtures were assembled in 100 mM NaCl with 5 mM MgCl2 in addition to standard reaction components as described in Material and Methods. Binding reactions were separated by sodium dodecyl sulfate–12% polyacrylamide gel electrophoresis, and unbound RNA was digested by adding 1 U of <t>RNase</t> <t>V1.</t> Signals were imaged with the Molecular Dynamics Storm PhosphorImager and quantified using ImageQuaNT version 4.2 software (Molecular Dynamics). The RNAs used to form the complexes are HTNV S-segment vRNA (lane 1), ORF RNA (lane 2), minipan RNA (lane 3), and Δ12 RNA (lane 4).
Rnase V1, supplied by Amano Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rnase v1/product/Amano Inc
Average 90 stars, based on 1 article reviews
rnase v1 - by Bioz Stars, 2026-04
90/100 stars
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rnase v1  (Promega)
90
Promega rnase v1
Analysis of the complex formed between HTNV N protein and deletion RNAs by UV cross-linking analysis. The concentration of N protein used in each binding reaction was 3.5 × 10−6 M. Reaction mixtures were assembled in 100 mM NaCl with 5 mM MgCl2 in addition to standard reaction components as described in Material and Methods. Binding reactions were separated by sodium dodecyl sulfate–12% polyacrylamide gel electrophoresis, and unbound RNA was digested by adding 1 U of <t>RNase</t> <t>V1.</t> Signals were imaged with the Molecular Dynamics Storm PhosphorImager and quantified using ImageQuaNT version 4.2 software (Molecular Dynamics). The RNAs used to form the complexes are HTNV S-segment vRNA (lane 1), ORF RNA (lane 2), minipan RNA (lane 3), and Δ12 RNA (lane 4).
Rnase V1, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rnase v1/product/Promega
Average 90 stars, based on 1 article reviews
rnase v1 - by Bioz Stars, 2026-04
90/100 stars
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Image Search Results


Analysis of the complex formed between HTNV N protein and deletion RNAs by UV cross-linking analysis. The concentration of N protein used in each binding reaction was 3.5 × 10−6 M. Reaction mixtures were assembled in 100 mM NaCl with 5 mM MgCl2 in addition to standard reaction components as described in Material and Methods. Binding reactions were separated by sodium dodecyl sulfate–12% polyacrylamide gel electrophoresis, and unbound RNA was digested by adding 1 U of RNase V1. Signals were imaged with the Molecular Dynamics Storm PhosphorImager and quantified using ImageQuaNT version 4.2 software (Molecular Dynamics). The RNAs used to form the complexes are HTNV S-segment vRNA (lane 1), ORF RNA (lane 2), minipan RNA (lane 3), and Δ12 RNA (lane 4).

Journal:

Article Title: cis -Acting Signals in Encapsidation of Hantaan Virus S-Segment Viral Genomic RNA by Its N Protein

doi: 10.1128/JVI.75.6.2646-2652.2001

Figure Lengend Snippet: Analysis of the complex formed between HTNV N protein and deletion RNAs by UV cross-linking analysis. The concentration of N protein used in each binding reaction was 3.5 × 10−6 M. Reaction mixtures were assembled in 100 mM NaCl with 5 mM MgCl2 in addition to standard reaction components as described in Material and Methods. Binding reactions were separated by sodium dodecyl sulfate–12% polyacrylamide gel electrophoresis, and unbound RNA was digested by adding 1 U of RNase V1. Signals were imaged with the Molecular Dynamics Storm PhosphorImager and quantified using ImageQuaNT version 4.2 software (Molecular Dynamics). The RNAs used to form the complexes are HTNV S-segment vRNA (lane 1), ORF RNA (lane 2), minipan RNA (lane 3), and Δ12 RNA (lane 4).

Article Snippet: Unbound RNA was digested by adding 1 U of RNase V1 (Amersham Pharmacia Biotech) or 50 U of RNase T1 (Ambion) and incubating for 30 min at 37°C.

Techniques: Concentration Assay, Binding Assay, Polyacrylamide Gel Electrophoresis, Software