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Covance
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GeneTex
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Image Search Results
Journal: The Journal of Cell Biology
Article Title: Cryo–electron tomography reveals a critical role of RIM1α in synaptic vesicle tethering
doi: 10.1083/jcb.201206063
Figure Lengend Snippet: Western blot analysis for ubiquitin and various presynaptic proteins. The double band detected for RIM1α corresponds to splice variants (see, e.g., Fig. 1 of ). MG132 induced an increase in the levels of RIM1α (WT), RIM1β (KO), and MUNC13 (WT and KO) and a smaller increase in RIM2 (KO) but not in other presynaptic proteins. Sample sizes (pairs of WT and KO littermates) are as follows: seven (RIM1 and MUNC13), six (RIM2), and three to four (ELKS, Liprin2, Liprin3, Rab3, synaptotagmin1, syntaxin1, SNAP25, VAMP2, and ubiquitin).
Article Snippet: Mouse antibodies against RIM1 (610907) and MUNC13 (610999) were purchased from BD, Rab3 (107011), syntaxin1 (110011), SNAP25 (111002), VAMP2 (104211), and ELKS (143003) were purchased from Synaptic Systems, synaptotagmin1 (136088) and ubiquitin (8017) were obtained from
Techniques: Western Blot