Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Nanoparticle-Based Lateral Flow Biosensors Integrated With Loop-Mediated Isothermal Amplification for the Rapid and Visual Diagnosis of Hepatitis B Virus in Clinical Application

doi: 10.3389/fbioe.2021.731415

Figure Lengend Snippet: Optimization of amplification temperature for the HBV-LAMP primer set. The LAMP reactions for the detection of HBV were monitored through real-time turbidity (LA-500), and the corresponding curves of DNA concentrations are displayed in the graph. The threshold value > 0.1 was considered as the positive result. 8 kinetic graphs were obtained at different temperatures (60–67°C; 1°C interval) with 100 IU target genomic DNA per reaction. The graphs from e to h show robust amplification. The optimal amplification temperature was 65°C.

Article Snippet: The real-time turbidimeter (LA-500) was obtained from Eiken Chemical Co., Ltd. (Japan).

Techniques: Amplification

Journal: Scientific Reports

Article Title: Development of a reverse transcription loop mediated isothermal amplification assay for the detection of Mouse reovirus type 3 in laboratory mice

doi: 10.1038/s41598-021-83034-1

Figure Lengend Snippet: Primer set optimization test for RT-LAMP assay by using Loopamp real-time turbidimeter. ( a ) RS1 turbidity curve for the tests of the Reo-3, SV, PVM, MHV, Ect, MVM, P. pneumotropica , B. bronchiseptica , K. pneumonia , P. aeruginosa and water, the turbidity curve was plotted using the software based on the Loopamp Realtime Turbidimeter.; ( b ) RS2 turbidity curve for all tests; ( c ) RS3 turbidity curve for all tests.

Article Snippet: The RT-LAMP reaction was performed in a Loopamp Realtime Turbidimeter (LA-200C; Eiken Chemical Co. Ltd., Tokyo, Japan) that measured the optical density of reactions every 6 sec at 650 nm (the reactions were considered positive when the turbidity reached ≥ 0.1 within 60 min).

Techniques: RT Lamp Assay, Software

Journal: Scientific Reports

Article Title: Development of a reverse transcription loop mediated isothermal amplification assay for the detection of Mouse reovirus type 3 in laboratory mice

doi: 10.1038/s41598-021-83034-1

Figure Lengend Snippet: The specificity of the RT-LAMP assay based on the RS2 primers. ( a ) The turbidity curves generated by the Loopamp real-time turbidimeter, resulting in tests of the Reo-3, SV, PVM, MHV, Ect, MVM, P. pneumotropica , B. bronchiseptica , K. pneumonia , P. aeruginosa and water. ( b ) RS2 amplification products visual detection for all specificity tests.

Article Snippet: The RT-LAMP reaction was performed in a Loopamp Realtime Turbidimeter (LA-200C; Eiken Chemical Co. Ltd., Tokyo, Japan) that measured the optical density of reactions every 6 sec at 650 nm (the reactions were considered positive when the turbidity reached ≥ 0.1 within 60 min).

Techniques: RT Lamp Assay, Generated, Amplification

Journal: Scientific Reports

Article Title: Development of a reverse transcription loop mediated isothermal amplification assay for the detection of Mouse reovirus type 3 in laboratory mice

doi: 10.1038/s41598-021-83034-1

Figure Lengend Snippet: The sensitivity of the RT-LAMP assay based on the RS2 primers. ( a ) The turbidity curves generated by the Loopamp real-time turbidimeter, resulting in the final nucleic acid concentration of 4 ng/μL, 400 pg/μL, 40 pg/μL, 4 pg/μL, 400 fg/μL, 40 fg/μL, 4 fg/μL, 400 ag/μL, 40 ag/μL and 4 ag/μL. ( b ) RS2 amplification products visual detection for all gradient dilution tests.

Article Snippet: The RT-LAMP reaction was performed in a Loopamp Realtime Turbidimeter (LA-200C; Eiken Chemical Co. Ltd., Tokyo, Japan) that measured the optical density of reactions every 6 sec at 650 nm (the reactions were considered positive when the turbidity reached ≥ 0.1 within 60 min).

Techniques: RT Lamp Assay, Generated, Nucleic Acid Concentration, Amplification