pvdf Search Results


pbs blocking buffer  (LI-COR)
99
LI-COR pbs blocking buffer
Procaspase 3 levels indicate lack of curcumin-induced apoptosis at the low, plasma level-informed curcumin concentration. (A) A549, H460, Caco-2, and HT29 cells treated with control, curcumin (4–50 µg/mL) for 24h, or staurosporine (2 µM, positive control) for 4h. Cells were washed with <t>PBS,</t> lysed, and processed for Western blotting as described in Materials and Methods. Each lane was loaded with 40 µg of total <t>protein.</t> <t>Membranes</t> were probed with primary antibodies against caspase 3 and either β-actin or tubulin (loading control), followed by IRDye 680 and 800-conjugated secondary antibodies. Blots were imaged using the ChemiDoc ™ platform. (B) Quantification of procaspase 3 band intensity was normalized to β-actin and expressed relative to the control for each cell line. Dot plots represent individual values from 3 biological replicates per cell line, with additional technical replicates included for A549 and HT29 (total n = 7), and for H460 and Caco-2 (total n = 4). Horizontal lines indicate the group mean and error bars represent standard deviation. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns = not significant. β-actin (ab8226) was used as the primary loading control following discontinuation of the tubulin antibody (ab59680); earlier tubulin-normalized blots are presented in the Supplementary Material .
Pbs Blocking Buffer, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
pbs blocking buffer - by Bioz Stars, 2026-04
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polyvinylidene fluoride pvdf blocking reagent  (Toyobo)
96
Toyobo polyvinylidene fluoride pvdf blocking reagent
Procaspase 3 levels indicate lack of curcumin-induced apoptosis at the low, plasma level-informed curcumin concentration. (A) A549, H460, Caco-2, and HT29 cells treated with control, curcumin (4–50 µg/mL) for 24h, or staurosporine (2 µM, positive control) for 4h. Cells were washed with <t>PBS,</t> lysed, and processed for Western blotting as described in Materials and Methods. Each lane was loaded with 40 µg of total <t>protein.</t> <t>Membranes</t> were probed with primary antibodies against caspase 3 and either β-actin or tubulin (loading control), followed by IRDye 680 and 800-conjugated secondary antibodies. Blots were imaged using the ChemiDoc ™ platform. (B) Quantification of procaspase 3 band intensity was normalized to β-actin and expressed relative to the control for each cell line. Dot plots represent individual values from 3 biological replicates per cell line, with additional technical replicates included for A549 and HT29 (total n = 7), and for H460 and Caco-2 (total n = 4). Horizontal lines indicate the group mean and error bars represent standard deviation. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns = not significant. β-actin (ab8226) was used as the primary loading control following discontinuation of the tubulin antibody (ab59680); earlier tubulin-normalized blots are presented in the Supplementary Material .
Polyvinylidene Fluoride Pvdf Blocking Reagent, supplied by Toyobo, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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li cor blocking buffer  (LI-COR)
99
LI-COR li cor blocking buffer
Procaspase 3 levels indicate lack of curcumin-induced apoptosis at the low, plasma level-informed curcumin concentration. (A) A549, H460, Caco-2, and HT29 cells treated with control, curcumin (4–50 µg/mL) for 24h, or staurosporine (2 µM, positive control) for 4h. Cells were washed with <t>PBS,</t> lysed, and processed for Western blotting as described in Materials and Methods. Each lane was loaded with 40 µg of total <t>protein.</t> <t>Membranes</t> were probed with primary antibodies against caspase 3 and either β-actin or tubulin (loading control), followed by IRDye 680 and 800-conjugated secondary antibodies. Blots were imaged using the ChemiDoc ™ platform. (B) Quantification of procaspase 3 band intensity was normalized to β-actin and expressed relative to the control for each cell line. Dot plots represent individual values from 3 biological replicates per cell line, with additional technical replicates included for A549 and HT29 (total n = 7), and for H460 and Caco-2 (total n = 4). Horizontal lines indicate the group mean and error bars represent standard deviation. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns = not significant. β-actin (ab8226) was used as the primary loading control following discontinuation of the tubulin antibody (ab59680); earlier tubulin-normalized blots are presented in the Supplementary Material .
Li Cor Blocking Buffer, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pvdf membrane  (LI-COR)
96
LI-COR pvdf membrane
Procaspase 3 levels indicate lack of curcumin-induced apoptosis at the low, plasma level-informed curcumin concentration. (A) A549, H460, Caco-2, and HT29 cells treated with control, curcumin (4–50 µg/mL) for 24h, or staurosporine (2 µM, positive control) for 4h. Cells were washed with <t>PBS,</t> lysed, and processed for Western blotting as described in Materials and Methods. Each lane was loaded with 40 µg of total <t>protein.</t> <t>Membranes</t> were probed with primary antibodies against caspase 3 and either β-actin or tubulin (loading control), followed by IRDye 680 and 800-conjugated secondary antibodies. Blots were imaged using the ChemiDoc ™ platform. (B) Quantification of procaspase 3 band intensity was normalized to β-actin and expressed relative to the control for each cell line. Dot plots represent individual values from 3 biological replicates per cell line, with additional technical replicates included for A549 and HT29 (total n = 7), and for H460 and Caco-2 (total n = 4). Horizontal lines indicate the group mean and error bars represent standard deviation. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns = not significant. β-actin (ab8226) was used as the primary loading control following discontinuation of the tubulin antibody (ab59680); earlier tubulin-normalized blots are presented in the Supplementary Material .
Pvdf Membrane, supplied by LI-COR, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
pvdf membrane - by Bioz Stars, 2026-04
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bio rad immunblot pvdf membrane  (Bio-Rad)
96
Bio-Rad bio rad immunblot pvdf membrane
Procaspase 3 levels indicate lack of curcumin-induced apoptosis at the low, plasma level-informed curcumin concentration. (A) A549, H460, Caco-2, and HT29 cells treated with control, curcumin (4–50 µg/mL) for 24h, or staurosporine (2 µM, positive control) for 4h. Cells were washed with <t>PBS,</t> lysed, and processed for Western blotting as described in Materials and Methods. Each lane was loaded with 40 µg of total <t>protein.</t> <t>Membranes</t> were probed with primary antibodies against caspase 3 and either β-actin or tubulin (loading control), followed by IRDye 680 and 800-conjugated secondary antibodies. Blots were imaged using the ChemiDoc ™ platform. (B) Quantification of procaspase 3 band intensity was normalized to β-actin and expressed relative to the control for each cell line. Dot plots represent individual values from 3 biological replicates per cell line, with additional technical replicates included for A549 and HT29 (total n = 7), and for H460 and Caco-2 (total n = 4). Horizontal lines indicate the group mean and error bars represent standard deviation. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns = not significant. β-actin (ab8226) was used as the primary loading control following discontinuation of the tubulin antibody (ab59680); earlier tubulin-normalized blots are presented in the Supplementary Material .
Bio Rad Immunblot Pvdf Membrane, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
bio rad immunblot pvdf membrane - by Bioz Stars, 2026-04
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lf pvdf transfer kit  (Bio-Rad)
95
Bio-Rad lf pvdf transfer kit
Procaspase 3 levels indicate lack of curcumin-induced apoptosis at the low, plasma level-informed curcumin concentration. (A) A549, H460, Caco-2, and HT29 cells treated with control, curcumin (4–50 µg/mL) for 24h, or staurosporine (2 µM, positive control) for 4h. Cells were washed with <t>PBS,</t> lysed, and processed for Western blotting as described in Materials and Methods. Each lane was loaded with 40 µg of total <t>protein.</t> <t>Membranes</t> were probed with primary antibodies against caspase 3 and either β-actin or tubulin (loading control), followed by IRDye 680 and 800-conjugated secondary antibodies. Blots were imaged using the ChemiDoc ™ platform. (B) Quantification of procaspase 3 band intensity was normalized to β-actin and expressed relative to the control for each cell line. Dot plots represent individual values from 3 biological replicates per cell line, with additional technical replicates included for A549 and HT29 (total n = 7), and for H460 and Caco-2 (total n = 4). Horizontal lines indicate the group mean and error bars represent standard deviation. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns = not significant. β-actin (ab8226) was used as the primary loading control following discontinuation of the tubulin antibody (ab59680); earlier tubulin-normalized blots are presented in the Supplementary Material .
Lf Pvdf Transfer Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lf pvdf transfer kit - by Bioz Stars, 2026-04
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pvdf membrane  (Bio-Rad)
96
Bio-Rad pvdf membrane
Procaspase 3 levels indicate lack of curcumin-induced apoptosis at the low, plasma level-informed curcumin concentration. (A) A549, H460, Caco-2, and HT29 cells treated with control, curcumin (4–50 µg/mL) for 24h, or staurosporine (2 µM, positive control) for 4h. Cells were washed with <t>PBS,</t> lysed, and processed for Western blotting as described in Materials and Methods. Each lane was loaded with 40 µg of total <t>protein.</t> <t>Membranes</t> were probed with primary antibodies against caspase 3 and either β-actin or tubulin (loading control), followed by IRDye 680 and 800-conjugated secondary antibodies. Blots were imaged using the ChemiDoc ™ platform. (B) Quantification of procaspase 3 band intensity was normalized to β-actin and expressed relative to the control for each cell line. Dot plots represent individual values from 3 biological replicates per cell line, with additional technical replicates included for A549 and HT29 (total n = 7), and for H460 and Caco-2 (total n = 4). Horizontal lines indicate the group mean and error bars represent standard deviation. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns = not significant. β-actin (ab8226) was used as the primary loading control following discontinuation of the tubulin antibody (ab59680); earlier tubulin-normalized blots are presented in the Supplementary Material .
Pvdf Membrane, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pvdf membrane/product/Bio-Rad
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trans blot turbomidi 0 2 mmpcdf transfer packs  (Bio-Rad)
96
Bio-Rad trans blot turbomidi 0 2 mmpcdf transfer packs
Procaspase 3 levels indicate lack of curcumin-induced apoptosis at the low, plasma level-informed curcumin concentration. (A) A549, H460, Caco-2, and HT29 cells treated with control, curcumin (4–50 µg/mL) for 24h, or staurosporine (2 µM, positive control) for 4h. Cells were washed with <t>PBS,</t> lysed, and processed for Western blotting as described in Materials and Methods. Each lane was loaded with 40 µg of total <t>protein.</t> <t>Membranes</t> were probed with primary antibodies against caspase 3 and either β-actin or tubulin (loading control), followed by IRDye 680 and 800-conjugated secondary antibodies. Blots were imaged using the ChemiDoc ™ platform. (B) Quantification of procaspase 3 band intensity was normalized to β-actin and expressed relative to the control for each cell line. Dot plots represent individual values from 3 biological replicates per cell line, with additional technical replicates included for A549 and HT29 (total n = 7), and for H460 and Caco-2 (total n = 4). Horizontal lines indicate the group mean and error bars represent standard deviation. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns = not significant. β-actin (ab8226) was used as the primary loading control following discontinuation of the tubulin antibody (ab59680); earlier tubulin-normalized blots are presented in the Supplementary Material .
Trans Blot Turbomidi 0 2 Mmpcdf Transfer Packs, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pvdf  (Bio-Rad)
96
Bio-Rad pvdf
Procaspase 3 levels indicate lack of curcumin-induced apoptosis at the low, plasma level-informed curcumin concentration. (A) A549, H460, Caco-2, and HT29 cells treated with control, curcumin (4–50 µg/mL) for 24h, or staurosporine (2 µM, positive control) for 4h. Cells were washed with <t>PBS,</t> lysed, and processed for Western blotting as described in Materials and Methods. Each lane was loaded with 40 µg of total <t>protein.</t> <t>Membranes</t> were probed with primary antibodies against caspase 3 and either β-actin or tubulin (loading control), followed by IRDye 680 and 800-conjugated secondary antibodies. Blots were imaged using the ChemiDoc ™ platform. (B) Quantification of procaspase 3 band intensity was normalized to β-actin and expressed relative to the control for each cell line. Dot plots represent individual values from 3 biological replicates per cell line, with additional technical replicates included for A549 and HT29 (total n = 7), and for H460 and Caco-2 (total n = 4). Horizontal lines indicate the group mean and error bars represent standard deviation. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns = not significant. β-actin (ab8226) was used as the primary loading control following discontinuation of the tubulin antibody (ab59680); earlier tubulin-normalized blots are presented in the Supplementary Material .
Pvdf, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pvdf membranes  (Bio-Rad)
96
Bio-Rad pvdf membranes
Procaspase 3 levels indicate lack of curcumin-induced apoptosis at the low, plasma level-informed curcumin concentration. (A) A549, H460, Caco-2, and HT29 cells treated with control, curcumin (4–50 µg/mL) for 24h, or staurosporine (2 µM, positive control) for 4h. Cells were washed with <t>PBS,</t> lysed, and processed for Western blotting as described in Materials and Methods. Each lane was loaded with 40 µg of total <t>protein.</t> <t>Membranes</t> were probed with primary antibodies against caspase 3 and either β-actin or tubulin (loading control), followed by IRDye 680 and 800-conjugated secondary antibodies. Blots were imaged using the ChemiDoc ™ platform. (B) Quantification of procaspase 3 band intensity was normalized to β-actin and expressed relative to the control for each cell line. Dot plots represent individual values from 3 biological replicates per cell line, with additional technical replicates included for A549 and HT29 (total n = 7), and for H460 and Caco-2 (total n = 4). Horizontal lines indicate the group mean and error bars represent standard deviation. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns = not significant. β-actin (ab8226) was used as the primary loading control following discontinuation of the tubulin antibody (ab59680); earlier tubulin-normalized blots are presented in the Supplementary Material .
Pvdf Membranes, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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polyvinylidene fluoride membrane  (Bio-Rad)
96
Bio-Rad polyvinylidene fluoride membrane
Procaspase 3 levels indicate lack of curcumin-induced apoptosis at the low, plasma level-informed curcumin concentration. (A) A549, H460, Caco-2, and HT29 cells treated with control, curcumin (4–50 µg/mL) for 24h, or staurosporine (2 µM, positive control) for 4h. Cells were washed with <t>PBS,</t> lysed, and processed for Western blotting as described in Materials and Methods. Each lane was loaded with 40 µg of total <t>protein.</t> <t>Membranes</t> were probed with primary antibodies against caspase 3 and either β-actin or tubulin (loading control), followed by IRDye 680 and 800-conjugated secondary antibodies. Blots were imaged using the ChemiDoc ™ platform. (B) Quantification of procaspase 3 band intensity was normalized to β-actin and expressed relative to the control for each cell line. Dot plots represent individual values from 3 biological replicates per cell line, with additional technical replicates included for A549 and HT29 (total n = 7), and for H460 and Caco-2 (total n = 4). Horizontal lines indicate the group mean and error bars represent standard deviation. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns = not significant. β-actin (ab8226) was used as the primary loading control following discontinuation of the tubulin antibody (ab59680); earlier tubulin-normalized blots are presented in the Supplementary Material .
Polyvinylidene Fluoride Membrane, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti rabbit 800  (LI-COR)
98
LI-COR anti rabbit 800
Procaspase 3 levels indicate lack of curcumin-induced apoptosis at the low, plasma level-informed curcumin concentration. (A) A549, H460, Caco-2, and HT29 cells treated with control, curcumin (4–50 µg/mL) for 24h, or staurosporine (2 µM, positive control) for 4h. Cells were washed with <t>PBS,</t> lysed, and processed for Western blotting as described in Materials and Methods. Each lane was loaded with 40 µg of total <t>protein.</t> <t>Membranes</t> were probed with primary antibodies against caspase 3 and either β-actin or tubulin (loading control), followed by IRDye 680 and 800-conjugated secondary antibodies. Blots were imaged using the ChemiDoc ™ platform. (B) Quantification of procaspase 3 band intensity was normalized to β-actin and expressed relative to the control for each cell line. Dot plots represent individual values from 3 biological replicates per cell line, with additional technical replicates included for A549 and HT29 (total n = 7), and for H460 and Caco-2 (total n = 4). Horizontal lines indicate the group mean and error bars represent standard deviation. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns = not significant. β-actin (ab8226) was used as the primary loading control following discontinuation of the tubulin antibody (ab59680); earlier tubulin-normalized blots are presented in the Supplementary Material .
Anti Rabbit 800, supplied by LI-COR, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Procaspase 3 levels indicate lack of curcumin-induced apoptosis at the low, plasma level-informed curcumin concentration. (A) A549, H460, Caco-2, and HT29 cells treated with control, curcumin (4–50 µg/mL) for 24h, or staurosporine (2 µM, positive control) for 4h. Cells were washed with PBS, lysed, and processed for Western blotting as described in Materials and Methods. Each lane was loaded with 40 µg of total protein. Membranes were probed with primary antibodies against caspase 3 and either β-actin or tubulin (loading control), followed by IRDye 680 and 800-conjugated secondary antibodies. Blots were imaged using the ChemiDoc ™ platform. (B) Quantification of procaspase 3 band intensity was normalized to β-actin and expressed relative to the control for each cell line. Dot plots represent individual values from 3 biological replicates per cell line, with additional technical replicates included for A549 and HT29 (total n = 7), and for H460 and Caco-2 (total n = 4). Horizontal lines indicate the group mean and error bars represent standard deviation. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns = not significant. β-actin (ab8226) was used as the primary loading control following discontinuation of the tubulin antibody (ab59680); earlier tubulin-normalized blots are presented in the Supplementary Material .

Journal: Pharmaceutical Biology

Article Title: Low, plasma level‑informed native curcumin concentrations fail to induce cell death in human lung and colorectal cancer cells

doi: 10.1080/13880209.2026.2640678

Figure Lengend Snippet: Procaspase 3 levels indicate lack of curcumin-induced apoptosis at the low, plasma level-informed curcumin concentration. (A) A549, H460, Caco-2, and HT29 cells treated with control, curcumin (4–50 µg/mL) for 24h, or staurosporine (2 µM, positive control) for 4h. Cells were washed with PBS, lysed, and processed for Western blotting as described in Materials and Methods. Each lane was loaded with 40 µg of total protein. Membranes were probed with primary antibodies against caspase 3 and either β-actin or tubulin (loading control), followed by IRDye 680 and 800-conjugated secondary antibodies. Blots were imaged using the ChemiDoc ™ platform. (B) Quantification of procaspase 3 band intensity was normalized to β-actin and expressed relative to the control for each cell line. Dot plots represent individual values from 3 biological replicates per cell line, with additional technical replicates included for A549 and HT29 (total n = 7), and for H460 and Caco-2 (total n = 4). Horizontal lines indicate the group mean and error bars represent standard deviation. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns = not significant. β-actin (ab8226) was used as the primary loading control following discontinuation of the tubulin antibody (ab59680); earlier tubulin-normalized blots are presented in the Supplementary Material .

Article Snippet: Complete Mini Protease Inhibitor Cocktail Tablet was obtained from Roche; the BCA Protein Assay Kit from Thermo Fisher Scientific; 4–20% SDS-PAGE Gels from Bio-Rad; PVDF membranes from Merck; and Intercept TM (PBS) Blocking Buffer from LI-COR Biosciences.

Techniques: Clinical Proteomics, Concentration Assay, Control, Positive Control, Western Blot, Standard Deviation