Journal: Oncology Reports

Article Title: microRNA-1301-mediated inhibition of tumorigenesis

doi: 10.3892/or.2011.1589

Figure Lengend Snippet: miR-1301 inhibits the migration of HepG2 cells in a scratch test. The scratch test was used to investigate the wound healing ability at 0, 12, 24 and 48 h. We observed that the proliferation and migration ability of HepG2 cells in the wounded area was reduced in the miR-1301 group after a 24 and 48 h repair period when compared with the control group. There was no difference between the miR-1301 transfected and the control group at 12 h.

Article Snippet: Total RNA was extracted from human HepG2 cell lines using TRIzol reagent (Takara Biotechnology Co., Ltd.).

Techniques: Migration, Transfection

Journal: Oncology Reports

Article Title: microRNA-1301-mediated inhibition of tumorigenesis

doi: 10.3892/or.2011.1589

Figure Lengend Snippet: miR-1301 inhibits the invasion of HepG2 cells in the Transwell chamber assay. Cell invasion ability was analyzed by the Transwell chamber assay 48 h after miR-1301 transfection. The results showed that the number of migration cells in the miR-1301 group was less than that in control group. This indicated that the invasion ability of HepG2 cells might be inhibited by miR-1301.

Article Snippet: Total RNA was extracted from human HepG2 cell lines using TRIzol reagent (Takara Biotechnology Co., Ltd.).

Techniques: Transwell Chamber Assay, Transfection, Migration

Journal: Oncology Reports

Article Title: microRNA-1301-mediated inhibition of tumorigenesis

doi: 10.3892/or.2011.1589

Figure Lengend Snippet: miR-1301 inhibits cell proliferation. The MTT assay was performed to monitor the proliferation rate of HepG2 cells after miR-1301 transfection. The optical density of each well was measured with a microplate spectrophotometer at 490 nm. The optical density of the miR-1301 group decreased at 24 h (A), 48 h (B), and 72 h (C) after transfection. The proliferation rate of cells decreased with increasing concentrations of inhibitor and increasing transfection time (D).

Article Snippet: Total RNA was extracted from human HepG2 cell lines using TRIzol reagent (Takara Biotechnology Co., Ltd.).

Techniques: MTT Assay, Transfection, Spectrophotometry

Journal: Oncology Reports

Article Title: microRNA-1301-mediated inhibition of tumorigenesis

doi: 10.3892/or.2011.1589

Figure Lengend Snippet: miR-1301 promotes cell apoptosis. Apoptosis of HepG2 cells was observed using fluorescence microscopy through a dual pass filter allowing to visualize the Annexin-V-FITC positive and the propidium iodide positive cells in the same field. The results showed that apoptosis of HepG2 cells increased 48 h after transfection of miR-1301 mimics in the miR-1301 group (Aa) when compared with the control group (Ba). (Aa) Apoptosis cells of the Annexin-V-FITC positive cells in the miR-1301 group; (Ab) cells excited by normal light in the miR-1301 group; (Ba) apoptosis cells of the Annexin-V-FITC positive cells in the control group; (Bb) cells excited by normal light in the control group.

Article Snippet: Total RNA was extracted from human HepG2 cell lines using TRIzol reagent (Takara Biotechnology Co., Ltd.).

Techniques: Fluorescence, Microscopy, Transfection