plb Search Results


chlorogenic acid chl  (LGC Standards)
90
LGC Standards chlorogenic acid chl
Effects of almond skin extract (AE) and <t>CHL</t> on SIR2 ( a ), SOD1 ( b ) and SOD2 ( c ) gene expression determined by RT-qPCR. Expression was normalized with TUB1 gene and expressed relative to young yeast cells (Y). Y CTL was young yeast (on day 2 of cultivation) and A CTL was aged yeast (on day 5 of cultivation). AE, almond extract (1 mg/mL). CHL: <t>chlorogenic</t> acid at 3 concentrations (CHL5, CHL10 and CHL25 corresponding to chlorogenic acid addition at 5, 10 and 25 µM, respectively). E-Resveratrol (RES, 10 µM) was used as control antiaging drug. Values are means ± standard deviations (SD) of 4 independent experiments. Different letters represent significant differences between the various extraction conditions ( p < 0.05).
Chlorogenic Acid Chl, supplied by LGC Standards, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hy 125017 blu667 mce  (MedChemExpress)
92
MedChemExpress hy 125017 blu667 mce
Effects of almond skin extract (AE) and <t>CHL</t> on SIR2 ( a ), SOD1 ( b ) and SOD2 ( c ) gene expression determined by RT-qPCR. Expression was normalized with TUB1 gene and expressed relative to young yeast cells (Y). Y CTL was young yeast (on day 2 of cultivation) and A CTL was aged yeast (on day 5 of cultivation). AE, almond extract (1 mg/mL). CHL: <t>chlorogenic</t> acid at 3 concentrations (CHL5, CHL10 and CHL25 corresponding to chlorogenic acid addition at 5, 10 and 25 µM, respectively). E-Resveratrol (RES, 10 µM) was used as control antiaging drug. Values are means ± standard deviations (SD) of 4 independent experiments. Different letters represent significant differences between the various extraction conditions ( p < 0.05).
Hy 125017 Blu667 Mce, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plb lentiviral vector  (Addgene inc)
93
Addgene inc plb lentiviral vector
Effects of almond skin extract (AE) and <t>CHL</t> on SIR2 ( a ), SOD1 ( b ) and SOD2 ( c ) gene expression determined by RT-qPCR. Expression was normalized with TUB1 gene and expressed relative to young yeast cells (Y). Y CTL was young yeast (on day 2 of cultivation) and A CTL was aged yeast (on day 5 of cultivation). AE, almond extract (1 mg/mL). CHL: <t>chlorogenic</t> acid at 3 concentrations (CHL5, CHL10 and CHL25 corresponding to chlorogenic acid addition at 5, 10 and 25 µM, respectively). E-Resveratrol (RES, 10 µM) was used as control antiaging drug. Values are means ± standard deviations (SD) of 4 independent experiments. Different letters represent significant differences between the various extraction conditions ( p < 0.05).
Plb Lentiviral Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plb  (DSMZ)
93
DSMZ plb
Effects of almond skin extract (AE) and <t>CHL</t> on SIR2 ( a ), SOD1 ( b ) and SOD2 ( c ) gene expression determined by RT-qPCR. Expression was normalized with TUB1 gene and expressed relative to young yeast cells (Y). Y CTL was young yeast (on day 2 of cultivation) and A CTL was aged yeast (on day 5 of cultivation). AE, almond extract (1 mg/mL). CHL: <t>chlorogenic</t> acid at 3 concentrations (CHL5, CHL10 and CHL25 corresponding to chlorogenic acid addition at 5, 10 and 25 µM, respectively). E-Resveratrol (RES, 10 µM) was used as control antiaging drug. Values are means ± standard deviations (SD) of 4 independent experiments. Different letters represent significant differences between the various extraction conditions ( p < 0.05).
Plb, supplied by DSMZ, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phospholamban gfp  (OriGene)
93
OriGene phospholamban gfp
Effects of almond skin extract (AE) and <t>CHL</t> on SIR2 ( a ), SOD1 ( b ) and SOD2 ( c ) gene expression determined by RT-qPCR. Expression was normalized with TUB1 gene and expressed relative to young yeast cells (Y). Y CTL was young yeast (on day 2 of cultivation) and A CTL was aged yeast (on day 5 of cultivation). AE, almond extract (1 mg/mL). CHL: <t>chlorogenic</t> acid at 3 concentrations (CHL5, CHL10 and CHL25 corresponding to chlorogenic acid addition at 5, 10 and 25 µM, respectively). E-Resveratrol (RES, 10 µM) was used as control antiaging drug. Values are means ± standard deviations (SD) of 4 independent experiments. Different letters represent significant differences between the various extraction conditions ( p < 0.05).
Phospholamban Gfp, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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apigenin  (LGC Standards)
93
LGC Standards apigenin
Effects of almond skin extract (AE) and <t>CHL</t> on SIR2 ( a ), SOD1 ( b ) and SOD2 ( c ) gene expression determined by RT-qPCR. Expression was normalized with TUB1 gene and expressed relative to young yeast cells (Y). Y CTL was young yeast (on day 2 of cultivation) and A CTL was aged yeast (on day 5 of cultivation). AE, almond extract (1 mg/mL). CHL: <t>chlorogenic</t> acid at 3 concentrations (CHL5, CHL10 and CHL25 corresponding to chlorogenic acid addition at 5, 10 and 25 µM, respectively). E-Resveratrol (RES, 10 µM) was used as control antiaging drug. Values are means ± standard deviations (SD) of 4 independent experiments. Different letters represent significant differences between the various extraction conditions ( p < 0.05).
Apigenin, supplied by LGC Standards, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1 3 stevioside 4 4 rebaudioside b 5 0 steviol  (LGC Standards)
93
LGC Standards 1 3 stevioside 4 4 rebaudioside b 5 0 steviol
Effects of almond skin extract (AE) and <t>CHL</t> on SIR2 ( a ), SOD1 ( b ) and SOD2 ( c ) gene expression determined by RT-qPCR. Expression was normalized with TUB1 gene and expressed relative to young yeast cells (Y). Y CTL was young yeast (on day 2 of cultivation) and A CTL was aged yeast (on day 5 of cultivation). AE, almond extract (1 mg/mL). CHL: <t>chlorogenic</t> acid at 3 concentrations (CHL5, CHL10 and CHL25 corresponding to chlorogenic acid addition at 5, 10 and 25 µM, respectively). E-Resveratrol (RES, 10 µM) was used as control antiaging drug. Values are means ± standard deviations (SD) of 4 independent experiments. Different letters represent significant differences between the various extraction conditions ( p < 0.05).
1 3 Stevioside 4 4 Rebaudioside B 5 0 Steviol, supplied by LGC Standards, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rebaudioside a 3 9 4 glucose 989 4200 steviol  (LGC Standards)
93
LGC Standards rebaudioside a 3 9 4 glucose 989 4200 steviol
Effects of almond skin extract (AE) and <t>CHL</t> on SIR2 ( a ), SOD1 ( b ) and SOD2 ( c ) gene expression determined by RT-qPCR. Expression was normalized with TUB1 gene and expressed relative to young yeast cells (Y). Y CTL was young yeast (on day 2 of cultivation) and A CTL was aged yeast (on day 5 of cultivation). AE, almond extract (1 mg/mL). CHL: <t>chlorogenic</t> acid at 3 concentrations (CHL5, CHL10 and CHL25 corresponding to chlorogenic acid addition at 5, 10 and 25 µM, respectively). E-Resveratrol (RES, 10 µM) was used as control antiaging drug. Values are means ± standard deviations (SD) of 4 independent experiments. Different letters represent significant differences between the various extraction conditions ( p < 0.05).
Rebaudioside A 3 9 4 Glucose 989 4200 Steviol, supplied by LGC Standards, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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medicagenic acid  (LGC Standards)
92
LGC Standards medicagenic acid
Effects of almond skin extract (AE) and <t>CHL</t> on SIR2 ( a ), SOD1 ( b ) and SOD2 ( c ) gene expression determined by RT-qPCR. Expression was normalized with TUB1 gene and expressed relative to young yeast cells (Y). Y CTL was young yeast (on day 2 of cultivation) and A CTL was aged yeast (on day 5 of cultivation). AE, almond extract (1 mg/mL). CHL: <t>chlorogenic</t> acid at 3 concentrations (CHL5, CHL10 and CHL25 corresponding to chlorogenic acid addition at 5, 10 and 25 µM, respectively). E-Resveratrol (RES, 10 µM) was used as control antiaging drug. Values are means ± standard deviations (SD) of 4 independent experiments. Different letters represent significant differences between the various extraction conditions ( p < 0.05).
Medicagenic Acid, supplied by LGC Standards, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plb sirna  (Santa Cruz Biotechnology)
85
Santa Cruz Biotechnology plb sirna
Effects of almond skin extract (AE) and <t>CHL</t> on SIR2 ( a ), SOD1 ( b ) and SOD2 ( c ) gene expression determined by RT-qPCR. Expression was normalized with TUB1 gene and expressed relative to young yeast cells (Y). Y CTL was young yeast (on day 2 of cultivation) and A CTL was aged yeast (on day 5 of cultivation). AE, almond extract (1 mg/mL). CHL: <t>chlorogenic</t> acid at 3 concentrations (CHL5, CHL10 and CHL25 corresponding to chlorogenic acid addition at 5, 10 and 25 µM, respectively). E-Resveratrol (RES, 10 µM) was used as control antiaging drug. Values are means ± standard deviations (SD) of 4 independent experiments. Different letters represent significant differences between the various extraction conditions ( p < 0.05).
Plb Sirna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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myricetin 3 5 7 trihydroxy 2 3 4 5 trihydroxyphenyl chromen 4 one  (LGC Standards)
90
LGC Standards myricetin 3 5 7 trihydroxy 2 3 4 5 trihydroxyphenyl chromen 4 one
Effects of almond skin extract (AE) and <t>CHL</t> on SIR2 ( a ), SOD1 ( b ) and SOD2 ( c ) gene expression determined by RT-qPCR. Expression was normalized with TUB1 gene and expressed relative to young yeast cells (Y). Y CTL was young yeast (on day 2 of cultivation) and A CTL was aged yeast (on day 5 of cultivation). AE, almond extract (1 mg/mL). CHL: <t>chlorogenic</t> acid at 3 concentrations (CHL5, CHL10 and CHL25 corresponding to chlorogenic acid addition at 5, 10 and 25 µM, respectively). E-Resveratrol (RES, 10 µM) was used as control antiaging drug. Values are means ± standard deviations (SD) of 4 independent experiments. Different letters represent significant differences between the various extraction conditions ( p < 0.05).
Myricetin 3 5 7 Trihydroxy 2 3 4 5 Trihydroxyphenyl Chromen 4 One, supplied by LGC Standards, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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catechin lgc standards  (LGC Standards)
91
LGC Standards catechin lgc standards
Effects of almond skin extract (AE) and <t>CHL</t> on SIR2 ( a ), SOD1 ( b ) and SOD2 ( c ) gene expression determined by RT-qPCR. Expression was normalized with TUB1 gene and expressed relative to young yeast cells (Y). Y CTL was young yeast (on day 2 of cultivation) and A CTL was aged yeast (on day 5 of cultivation). AE, almond extract (1 mg/mL). CHL: <t>chlorogenic</t> acid at 3 concentrations (CHL5, CHL10 and CHL25 corresponding to chlorogenic acid addition at 5, 10 and 25 µM, respectively). E-Resveratrol (RES, 10 µM) was used as control antiaging drug. Values are means ± standard deviations (SD) of 4 independent experiments. Different letters represent significant differences between the various extraction conditions ( p < 0.05).
Catechin Lgc Standards, supplied by LGC Standards, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Effects of almond skin extract (AE) and CHL on SIR2 ( a ), SOD1 ( b ) and SOD2 ( c ) gene expression determined by RT-qPCR. Expression was normalized with TUB1 gene and expressed relative to young yeast cells (Y). Y CTL was young yeast (on day 2 of cultivation) and A CTL was aged yeast (on day 5 of cultivation). AE, almond extract (1 mg/mL). CHL: chlorogenic acid at 3 concentrations (CHL5, CHL10 and CHL25 corresponding to chlorogenic acid addition at 5, 10 and 25 µM, respectively). E-Resveratrol (RES, 10 µM) was used as control antiaging drug. Values are means ± standard deviations (SD) of 4 independent experiments. Different letters represent significant differences between the various extraction conditions ( p < 0.05).

Journal: Life

Article Title: Almond Skin Extracts and Chlorogenic Acid Delay Chronological Aging and Enhanced Oxidative Stress Response in Yeast

doi: 10.3390/life10060080

Figure Lengend Snippet: Effects of almond skin extract (AE) and CHL on SIR2 ( a ), SOD1 ( b ) and SOD2 ( c ) gene expression determined by RT-qPCR. Expression was normalized with TUB1 gene and expressed relative to young yeast cells (Y). Y CTL was young yeast (on day 2 of cultivation) and A CTL was aged yeast (on day 5 of cultivation). AE, almond extract (1 mg/mL). CHL: chlorogenic acid at 3 concentrations (CHL5, CHL10 and CHL25 corresponding to chlorogenic acid addition at 5, 10 and 25 µM, respectively). E-Resveratrol (RES, 10 µM) was used as control antiaging drug. Values are means ± standard deviations (SD) of 4 independent experiments. Different letters represent significant differences between the various extraction conditions ( p < 0.05).

Article Snippet: Chlorogenic acid (CHL) and resveratrol (RES) standard was purchased from LGC Standard (Molsheim, France).

Techniques: Gene Expression, Quantitative RT-PCR, Expressing, Control, Extraction

Effects of AE and CHL on SIRT1/SIR2 ( a ) and total SOD ( b ) enzyme activities. Y-CTL was young yeast (on day 2 of cultivation) and A-CTL was aged yeast (on day 5 of cultivation). AE, almond extract (1 mg/mL). CHL: chlorogenic acid at 3 concentrations (CHL5, CHL10 and CHL25 corresponding to chlorogenic acid addition at 5, 10 and 25 µM, respectively). E-Resveratrol (RES, 10 µM) was used as control antiaging drug. In young yeast culture (Y), actual SIRT1/SIR2 enzyme activity was equal to 4564.4 FU/mg protein, whereas total SOD activity was 35.6 units/mg protein. The enzyme activity was expressed as a percentage of Y-CTL. Values are means ± standard deviations (SD) of 4 independent experiments. Different letters represent significant differences between the various extraction conditions ( p < 0.05).

Journal: Life

Article Title: Almond Skin Extracts and Chlorogenic Acid Delay Chronological Aging and Enhanced Oxidative Stress Response in Yeast

doi: 10.3390/life10060080

Figure Lengend Snippet: Effects of AE and CHL on SIRT1/SIR2 ( a ) and total SOD ( b ) enzyme activities. Y-CTL was young yeast (on day 2 of cultivation) and A-CTL was aged yeast (on day 5 of cultivation). AE, almond extract (1 mg/mL). CHL: chlorogenic acid at 3 concentrations (CHL5, CHL10 and CHL25 corresponding to chlorogenic acid addition at 5, 10 and 25 µM, respectively). E-Resveratrol (RES, 10 µM) was used as control antiaging drug. In young yeast culture (Y), actual SIRT1/SIR2 enzyme activity was equal to 4564.4 FU/mg protein, whereas total SOD activity was 35.6 units/mg protein. The enzyme activity was expressed as a percentage of Y-CTL. Values are means ± standard deviations (SD) of 4 independent experiments. Different letters represent significant differences between the various extraction conditions ( p < 0.05).

Article Snippet: Chlorogenic acid (CHL) and resveratrol (RES) standard was purchased from LGC Standard (Molsheim, France).

Techniques: Control, Activity Assay, Extraction

Survival and ROS/RNS production under UV-C-induced oxidative stress conditions. ( a ) Survival assay, plates were incubated at 28 °C for 3 d and the number of colonies was counted; excepted if mentioned CTL was DBY746 yeast subjected to UV-C stress. ( b ) ROS/RNS production was evaluated using the dihydrorhodamine 123 (DHR123) probe. AE, almond extract (1 mg/mL). CHL: chlorogenic acid at 3 concentrations (CHL5, CHL10 and CHL25 corresponding to chlorogenic acid addition at 5, 10 and 25 µM, respectively). E-Resveratrol (RES, 10 µM) was used as control antiaging drug. Values are means ± standard deviations (SD) of 6 independent experiments. Different letters represent significant differences between the various extraction conditions ( p < 0.05).

Journal: Life

Article Title: Almond Skin Extracts and Chlorogenic Acid Delay Chronological Aging and Enhanced Oxidative Stress Response in Yeast

doi: 10.3390/life10060080

Figure Lengend Snippet: Survival and ROS/RNS production under UV-C-induced oxidative stress conditions. ( a ) Survival assay, plates were incubated at 28 °C for 3 d and the number of colonies was counted; excepted if mentioned CTL was DBY746 yeast subjected to UV-C stress. ( b ) ROS/RNS production was evaluated using the dihydrorhodamine 123 (DHR123) probe. AE, almond extract (1 mg/mL). CHL: chlorogenic acid at 3 concentrations (CHL5, CHL10 and CHL25 corresponding to chlorogenic acid addition at 5, 10 and 25 µM, respectively). E-Resveratrol (RES, 10 µM) was used as control antiaging drug. Values are means ± standard deviations (SD) of 6 independent experiments. Different letters represent significant differences between the various extraction conditions ( p < 0.05).

Article Snippet: Chlorogenic acid (CHL) and resveratrol (RES) standard was purchased from LGC Standard (Molsheim, France).

Techniques: Clonogenic Cell Survival Assay, Incubation, Control, Extraction

Schematic view of the different ROS/RNS-induced oxidative damages to membrane lipids, proteins and DNA ( a ), and effects of AE and CHL on malondialdehyde (MDA) levels measured by TBARS assay ( b ), protein carbonyl contents determined by ELISA assay ( c ) and 8-oxo-Guanine formation determined by ELISA assay ( d ). AE, almond extract (1 mg/mL). CHL: chlorogenic acid at 3 concentrations (CHL5, CHL10 and CHL25 corresponding to chlorogenic acid addition at 5, 10 and 25 µM, respectively). E-Resveratrol (RES, 10 µM) was used as control antiaging drug. Values are means ± standard deviations (SD) of 6 independent experiments. Different letters represent significant differences between the various extraction conditions ( p < 0.05).

Journal: Life

Article Title: Almond Skin Extracts and Chlorogenic Acid Delay Chronological Aging and Enhanced Oxidative Stress Response in Yeast

doi: 10.3390/life10060080

Figure Lengend Snippet: Schematic view of the different ROS/RNS-induced oxidative damages to membrane lipids, proteins and DNA ( a ), and effects of AE and CHL on malondialdehyde (MDA) levels measured by TBARS assay ( b ), protein carbonyl contents determined by ELISA assay ( c ) and 8-oxo-Guanine formation determined by ELISA assay ( d ). AE, almond extract (1 mg/mL). CHL: chlorogenic acid at 3 concentrations (CHL5, CHL10 and CHL25 corresponding to chlorogenic acid addition at 5, 10 and 25 µM, respectively). E-Resveratrol (RES, 10 µM) was used as control antiaging drug. Values are means ± standard deviations (SD) of 6 independent experiments. Different letters represent significant differences between the various extraction conditions ( p < 0.05).

Article Snippet: Chlorogenic acid (CHL) and resveratrol (RES) standard was purchased from LGC Standard (Molsheim, France).

Techniques: Membrane, TBARS Assay, Enzyme-linked Immunosorbent Assay, Control, Extraction