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Addgene inc
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Addgene inc
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Image Search Results
Journal: Experimental and Therapeutic Medicine
Article Title: C1QBP is upregulated in colon cancer and binds to apolipoprotein A-I
doi: 10.3892/etm.2017.4249
Figure Lengend Snippet: SDS-PAGE gel after C1QBP immunoprecipitation. Immunoprecipitation of whole cell lysates from 293T cells overexpressing C1QBP (pFLAG_CMV2_C1QBP) was performed using FLAG antibodies. The FLAG-tagged C1QBP protein was immunoprecipitated efficiently using anti-FLAG antibodies and stained in a ‘2-10’ numbered slice of an SDS-PAGE gel. 293T cells overexpressing only FLAG (pFLAG_CMV2_MOCK_293T) were used in control immunoprecipitation. The indicated slices were excised from SDS-PAGE gels and subjected to mass analyses to identify the proteins that co-precipitated with C1QBP. C1QBP, complement component 1, q subcomponent-binding protein.
Article Snippet: The amplified PCR product was digested using EcoRI-XmaI (Clontech Laboratories), then inserted into
Techniques: SDS Page, Immunoprecipitation, Staining, Binding Assay