pet30a vector Search Results


pet30a  (Promega)
90
Promega pet30a
Duration of the expression of His- FIP-fve fusion protein. Lysates were obtained from E. coli (DE3) cells with <t>pET30a-</t> FIP-fve induced by 1 mM Isopropyl β-d-thiogalactoside (IPTG) and were analyzed on 12% SDS-PAGE. Lane M was a molecular weight marker. Lanes 1 to 4 were the samples induced for 0, 1.5, 3.0, and 4.5 h, respectively. The arrow indicated the target protein His- FIP-fve . 10 μL sample was used in SDS-PAGE after centrifugation.
Pet30a, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pet30a/product/Promega
Average 90 stars, based on 1 article reviews
pet30a - by Bioz Stars, 2026-04
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pet-30a-smaldh_312 vector  (LGC Genomics GmbH)
90
LGC Genomics GmbH pet-30a-smaldh_312 vector
Duration of the expression of His- FIP-fve fusion protein. Lysates were obtained from E. coli (DE3) cells with <t>pET30a-</t> FIP-fve induced by 1 mM Isopropyl β-d-thiogalactoside (IPTG) and were analyzed on 12% SDS-PAGE. Lane M was a molecular weight marker. Lanes 1 to 4 were the samples induced for 0, 1.5, 3.0, and 4.5 h, respectively. The arrow indicated the target protein His- FIP-fve . 10 μL sample was used in SDS-PAGE after centrifugation.
Pet 30a Smaldh 312 Vector, supplied by LGC Genomics GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pet-30a-smaldh_312 vector/product/LGC Genomics GmbH
Average 90 stars, based on 1 article reviews
pet-30a-smaldh_312 vector - by Bioz Stars, 2026-04
90/100 stars
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pet30a (+) vector  (Taihe Biotechnology Co Ltd)
90
Taihe Biotechnology Co Ltd pet30a (+) vector
Duration of the expression of His- FIP-fve fusion protein. Lysates were obtained from E. coli (DE3) cells with <t>pET30a-</t> FIP-fve induced by 1 mM Isopropyl β-d-thiogalactoside (IPTG) and were analyzed on 12% SDS-PAGE. Lane M was a molecular weight marker. Lanes 1 to 4 were the samples induced for 0, 1.5, 3.0, and 4.5 h, respectively. The arrow indicated the target protein His- FIP-fve . 10 μL sample was used in SDS-PAGE after centrifugation.
Pet30a (+) Vector, supplied by Taihe Biotechnology Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pet30a (+) vector/product/Taihe Biotechnology Co Ltd
Average 90 stars, based on 1 article reviews
pet30a (+) vector - by Bioz Stars, 2026-04
90/100 stars
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pet-30a(+) expression vector  (Solarbio Inc)
90
Solarbio Inc pet-30a(+) expression vector
Duration of the expression of His- FIP-fve fusion protein. Lysates were obtained from E. coli (DE3) cells with <t>pET30a-</t> FIP-fve induced by 1 mM Isopropyl β-d-thiogalactoside (IPTG) and were analyzed on 12% SDS-PAGE. Lane M was a molecular weight marker. Lanes 1 to 4 were the samples induced for 0, 1.5, 3.0, and 4.5 h, respectively. The arrow indicated the target protein His- FIP-fve . 10 μL sample was used in SDS-PAGE after centrifugation.
Pet 30a(+) Expression Vector, supplied by Solarbio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pet-30a(+) expression vector/product/Solarbio Inc
Average 90 stars, based on 1 article reviews
pet-30a(+) expression vector - by Bioz Stars, 2026-04
90/100 stars
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pet30a vector system  (Merck & Co)
90
Merck & Co pet30a vector system
Duration of the expression of His- FIP-fve fusion protein. Lysates were obtained from E. coli (DE3) cells with <t>pET30a-</t> FIP-fve induced by 1 mM Isopropyl β-d-thiogalactoside (IPTG) and were analyzed on 12% SDS-PAGE. Lane M was a molecular weight marker. Lanes 1 to 4 were the samples induced for 0, 1.5, 3.0, and 4.5 h, respectively. The arrow indicated the target protein His- FIP-fve . 10 μL sample was used in SDS-PAGE after centrifugation.
Pet30a Vector System, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pet30a vector system/product/Merck & Co
Average 90 stars, based on 1 article reviews
pet30a vector system - by Bioz Stars, 2026-04
90/100 stars
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expression vector pet30a  (Beijing Protein Innovation)
90
Beijing Protein Innovation expression vector pet30a
Duration of the expression of His- FIP-fve fusion protein. Lysates were obtained from E. coli (DE3) cells with <t>pET30a-</t> FIP-fve induced by 1 mM Isopropyl β-d-thiogalactoside (IPTG) and were analyzed on 12% SDS-PAGE. Lane M was a molecular weight marker. Lanes 1 to 4 were the samples induced for 0, 1.5, 3.0, and 4.5 h, respectively. The arrow indicated the target protein His- FIP-fve . 10 μL sample was used in SDS-PAGE after centrifugation.
Expression Vector Pet30a, supplied by Beijing Protein Innovation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/expression vector pet30a/product/Beijing Protein Innovation
Average 90 stars, based on 1 article reviews
expression vector pet30a - by Bioz Stars, 2026-04
90/100 stars
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pet30(a) vectors  (Quintara Discovery)
90
Quintara Discovery pet30(a) vectors
Duration of the expression of His- FIP-fve fusion protein. Lysates were obtained from E. coli (DE3) cells with <t>pET30a-</t> FIP-fve induced by 1 mM Isopropyl β-d-thiogalactoside (IPTG) and were analyzed on 12% SDS-PAGE. Lane M was a molecular weight marker. Lanes 1 to 4 were the samples induced for 0, 1.5, 3.0, and 4.5 h, respectively. The arrow indicated the target protein His- FIP-fve . 10 μL sample was used in SDS-PAGE after centrifugation.
Pet30(A) Vectors, supplied by Quintara Discovery, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pet30(a) vectors/product/Quintara Discovery
Average 90 stars, based on 1 article reviews
pet30(a) vectors - by Bioz Stars, 2026-04
90/100 stars
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vector pet30a  (ACGT Inc)
90
ACGT Inc vector pet30a
Duration of the expression of His- FIP-fve fusion protein. Lysates were obtained from E. coli (DE3) cells with <t>pET30a-</t> FIP-fve induced by 1 mM Isopropyl β-d-thiogalactoside (IPTG) and were analyzed on 12% SDS-PAGE. Lane M was a molecular weight marker. Lanes 1 to 4 were the samples induced for 0, 1.5, 3.0, and 4.5 h, respectively. The arrow indicated the target protein His- FIP-fve . 10 μL sample was used in SDS-PAGE after centrifugation.
Vector Pet30a, supplied by ACGT Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/vector pet30a/product/ACGT Inc
Average 90 stars, based on 1 article reviews
vector pet30a - by Bioz Stars, 2026-04
90/100 stars
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pet30a vector  (EZBiolab Inc)
90
EZBiolab Inc pet30a vector
Duration of the expression of His- FIP-fve fusion protein. Lysates were obtained from E. coli (DE3) cells with <t>pET30a-</t> FIP-fve induced by 1 mM Isopropyl β-d-thiogalactoside (IPTG) and were analyzed on 12% SDS-PAGE. Lane M was a molecular weight marker. Lanes 1 to 4 were the samples induced for 0, 1.5, 3.0, and 4.5 h, respectively. The arrow indicated the target protein His- FIP-fve . 10 μL sample was used in SDS-PAGE after centrifugation.
Pet30a Vector, supplied by EZBiolab Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pet30a vector/product/EZBiolab Inc
Average 90 stars, based on 1 article reviews
pet30a vector - by Bioz Stars, 2026-04
90/100 stars
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vector pet-30a(þ  (Merck & Co)
90
Merck & Co vector pet-30a(þ
Duration of the expression of His- FIP-fve fusion protein. Lysates were obtained from E. coli (DE3) cells with <t>pET30a-</t> FIP-fve induced by 1 mM Isopropyl β-d-thiogalactoside (IPTG) and were analyzed on 12% SDS-PAGE. Lane M was a molecular weight marker. Lanes 1 to 4 were the samples induced for 0, 1.5, 3.0, and 4.5 h, respectively. The arrow indicated the target protein His- FIP-fve . 10 μL sample was used in SDS-PAGE after centrifugation.
Vector Pet 30a(þ, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/vector pet-30a(þ/product/Merck & Co
Average 90 stars, based on 1 article reviews
vector pet-30a(þ - by Bioz Stars, 2026-04
90/100 stars
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pet-30a  (Merck & Co)
90
Merck & Co pet-30a
Duration of the expression of His- FIP-fve fusion protein. Lysates were obtained from E. coli (DE3) cells with <t>pET30a-</t> FIP-fve induced by 1 mM Isopropyl β-d-thiogalactoside (IPTG) and were analyzed on 12% SDS-PAGE. Lane M was a molecular weight marker. Lanes 1 to 4 were the samples induced for 0, 1.5, 3.0, and 4.5 h, respectively. The arrow indicated the target protein His- FIP-fve . 10 μL sample was used in SDS-PAGE after centrifugation.
Pet 30a, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pet-30a/product/Merck & Co
Average 90 stars, based on 1 article reviews
pet-30a - by Bioz Stars, 2026-04
90/100 stars
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Image Search Results


Duration of the expression of His- FIP-fve fusion protein. Lysates were obtained from E. coli (DE3) cells with pET30a- FIP-fve induced by 1 mM Isopropyl β-d-thiogalactoside (IPTG) and were analyzed on 12% SDS-PAGE. Lane M was a molecular weight marker. Lanes 1 to 4 were the samples induced for 0, 1.5, 3.0, and 4.5 h, respectively. The arrow indicated the target protein His- FIP-fve . 10 μL sample was used in SDS-PAGE after centrifugation.

Journal: International Journal of Molecular Sciences

Article Title: High-Yield Production in Escherichia coli of Fungal Immunomodulatory Protein Isolated from Flammulina velutipes and Its Bioactivity Assay in Vivo

doi: 10.3390/ijms14022230

Figure Lengend Snippet: Duration of the expression of His- FIP-fve fusion protein. Lysates were obtained from E. coli (DE3) cells with pET30a- FIP-fve induced by 1 mM Isopropyl β-d-thiogalactoside (IPTG) and were analyzed on 12% SDS-PAGE. Lane M was a molecular weight marker. Lanes 1 to 4 were the samples induced for 0, 1.5, 3.0, and 4.5 h, respectively. The arrow indicated the target protein His- FIP-fve . 10 μL sample was used in SDS-PAGE after centrifugation.

Article Snippet: The PCR product was gel purified and then ligated into pET30a (+) at the Bam HI (R602A, Promega) and Eco RI (R6011, Promega) sites.

Techniques: Expressing, SDS Page, Molecular Weight, Marker, Centrifugation