pet21d plasmid Search Results


d komander n a pet21d ube1  (Addgene inc)
93
Addgene inc d komander n a pet21d ube1
D Komander N A Pet21d Ube1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/d komander n a pet21d ube1/product/Addgene inc
Average 93 stars, based on 1 article reviews
d komander n a pet21d ube1 - by Bioz Stars, 2026-04
93/100 stars
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unresolved region  (Addgene inc)
93
Addgene inc unresolved region
(A) Three-dimensional structure of bovine RPE65 (PDB ID: 3FSN ). The crystallographically <t>unresolved</t> (aa107–125) region of RPE65 protein, modeled using I-TASSER server, is shown in red color. (B) Alignment of RPE65 aa107–125 from several jawed and jawless vertebrate taxa showing sequence conservation throughout evolution. (C) Helical wheel representation of the crystallographically unresolved aa107–125 region of RPE65 protein using HELIQUEST server. (D) Predicted secondary structure, relative accessibility and hydropathy analysis of the aa107–125 region of RPE65 protein using I-TASSER and ENDScript server, respectively. (E) Hydropathic moment plot of combined 35 different RPE65 aa107–125 and paralogous conserved region sequences from vertebrate and non-vertebrate carotenoid cleavage dioxygenase (CCD) paralogs, derived from HeliQuest . Red stars, RPE65 s; magenta circles, vertebrate BCO1s; blue circles, vertebrate BCO2s; black circle, early chordate ( Ciona ) CCD; yellow circles, arthropod ( Trinorchestia , Palaemon , Drosophila , and Galleria ) CCDs; and green circles, nematode ( Caenorhabditis ) -CCDs. Note tight grouping of all 10 RPE65 s distinct from other paralogs. (F) Physicochemical properties of the crystallographically unresolved (aa107–125) of RPE65 protein. Abbreviations: acc, accessibility; hyd, hydrophobicity.
Unresolved Region, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/unresolved region/product/Addgene inc
Average 93 stars, based on 1 article reviews
unresolved region - by Bioz Stars, 2026-04
93/100 stars
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pet21d myc bira  (Addgene inc)
93
Addgene inc pet21d myc bira
(A) Three-dimensional structure of bovine RPE65 (PDB ID: 3FSN ). The crystallographically <t>unresolved</t> (aa107–125) region of RPE65 protein, modeled using I-TASSER server, is shown in red color. (B) Alignment of RPE65 aa107–125 from several jawed and jawless vertebrate taxa showing sequence conservation throughout evolution. (C) Helical wheel representation of the crystallographically unresolved aa107–125 region of RPE65 protein using HELIQUEST server. (D) Predicted secondary structure, relative accessibility and hydropathy analysis of the aa107–125 region of RPE65 protein using I-TASSER and ENDScript server, respectively. (E) Hydropathic moment plot of combined 35 different RPE65 aa107–125 and paralogous conserved region sequences from vertebrate and non-vertebrate carotenoid cleavage dioxygenase (CCD) paralogs, derived from HeliQuest . Red stars, RPE65 s; magenta circles, vertebrate BCO1s; blue circles, vertebrate BCO2s; black circle, early chordate ( Ciona ) CCD; yellow circles, arthropod ( Trinorchestia , Palaemon , Drosophila , and Galleria ) CCDs; and green circles, nematode ( Caenorhabditis ) -CCDs. Note tight grouping of all 10 RPE65 s distinct from other paralogs. (F) Physicochemical properties of the crystallographically unresolved (aa107–125) of RPE65 protein. Abbreviations: acc, accessibility; hyd, hydrophobicity.
Pet21d Myc Bira, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pet21d myc bira/product/Addgene inc
Average 93 stars, based on 1 article reviews
pet21d myc bira - by Bioz Stars, 2026-04
93/100 stars
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vector pet 21d  (GenScript corporation)
90
GenScript corporation vector pet 21d
(A) Three-dimensional structure of bovine RPE65 (PDB ID: 3FSN ). The crystallographically <t>unresolved</t> (aa107–125) region of RPE65 protein, modeled using I-TASSER server, is shown in red color. (B) Alignment of RPE65 aa107–125 from several jawed and jawless vertebrate taxa showing sequence conservation throughout evolution. (C) Helical wheel representation of the crystallographically unresolved aa107–125 region of RPE65 protein using HELIQUEST server. (D) Predicted secondary structure, relative accessibility and hydropathy analysis of the aa107–125 region of RPE65 protein using I-TASSER and ENDScript server, respectively. (E) Hydropathic moment plot of combined 35 different RPE65 aa107–125 and paralogous conserved region sequences from vertebrate and non-vertebrate carotenoid cleavage dioxygenase (CCD) paralogs, derived from HeliQuest . Red stars, RPE65 s; magenta circles, vertebrate BCO1s; blue circles, vertebrate BCO2s; black circle, early chordate ( Ciona ) CCD; yellow circles, arthropod ( Trinorchestia , Palaemon , Drosophila , and Galleria ) CCDs; and green circles, nematode ( Caenorhabditis ) -CCDs. Note tight grouping of all 10 RPE65 s distinct from other paralogs. (F) Physicochemical properties of the crystallographically unresolved (aa107–125) of RPE65 protein. Abbreviations: acc, accessibility; hyd, hydrophobicity.
Vector Pet 21d, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/vector pet 21d/product/GenScript corporation
Average 90 stars, based on 1 article reviews
vector pet 21d - by Bioz Stars, 2026-04
90/100 stars
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pdtat.pet21d expression plasmid  (Promega)
90
Promega pdtat.pet21d expression plasmid
(A) Three-dimensional structure of bovine RPE65 (PDB ID: 3FSN ). The crystallographically <t>unresolved</t> (aa107–125) region of RPE65 protein, modeled using I-TASSER server, is shown in red color. (B) Alignment of RPE65 aa107–125 from several jawed and jawless vertebrate taxa showing sequence conservation throughout evolution. (C) Helical wheel representation of the crystallographically unresolved aa107–125 region of RPE65 protein using HELIQUEST server. (D) Predicted secondary structure, relative accessibility and hydropathy analysis of the aa107–125 region of RPE65 protein using I-TASSER and ENDScript server, respectively. (E) Hydropathic moment plot of combined 35 different RPE65 aa107–125 and paralogous conserved region sequences from vertebrate and non-vertebrate carotenoid cleavage dioxygenase (CCD) paralogs, derived from HeliQuest . Red stars, RPE65 s; magenta circles, vertebrate BCO1s; blue circles, vertebrate BCO2s; black circle, early chordate ( Ciona ) CCD; yellow circles, arthropod ( Trinorchestia , Palaemon , Drosophila , and Galleria ) CCDs; and green circles, nematode ( Caenorhabditis ) -CCDs. Note tight grouping of all 10 RPE65 s distinct from other paralogs. (F) Physicochemical properties of the crystallographically unresolved (aa107–125) of RPE65 protein. Abbreviations: acc, accessibility; hyd, hydrophobicity.
Pdtat.Pet21d Expression Plasmid, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pdtat.pet21d expression plasmid/product/Promega
Average 90 stars, based on 1 article reviews
pdtat.pet21d expression plasmid - by Bioz Stars, 2026-04
90/100 stars
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recombinant pet21d(+) plasmid  (Qiagen)
90
Qiagen recombinant pet21d(+) plasmid
(A) Three-dimensional structure of bovine RPE65 (PDB ID: 3FSN ). The crystallographically <t>unresolved</t> (aa107–125) region of RPE65 protein, modeled using I-TASSER server, is shown in red color. (B) Alignment of RPE65 aa107–125 from several jawed and jawless vertebrate taxa showing sequence conservation throughout evolution. (C) Helical wheel representation of the crystallographically unresolved aa107–125 region of RPE65 protein using HELIQUEST server. (D) Predicted secondary structure, relative accessibility and hydropathy analysis of the aa107–125 region of RPE65 protein using I-TASSER and ENDScript server, respectively. (E) Hydropathic moment plot of combined 35 different RPE65 aa107–125 and paralogous conserved region sequences from vertebrate and non-vertebrate carotenoid cleavage dioxygenase (CCD) paralogs, derived from HeliQuest . Red stars, RPE65 s; magenta circles, vertebrate BCO1s; blue circles, vertebrate BCO2s; black circle, early chordate ( Ciona ) CCD; yellow circles, arthropod ( Trinorchestia , Palaemon , Drosophila , and Galleria ) CCDs; and green circles, nematode ( Caenorhabditis ) -CCDs. Note tight grouping of all 10 RPE65 s distinct from other paralogs. (F) Physicochemical properties of the crystallographically unresolved (aa107–125) of RPE65 protein. Abbreviations: acc, accessibility; hyd, hydrophobicity.
Recombinant Pet21d(+) Plasmid, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant pet21d(+) plasmid/product/Qiagen
Average 90 stars, based on 1 article reviews
recombinant pet21d(+) plasmid - by Bioz Stars, 2026-04
90/100 stars
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plasmid pet-21d  (Merck & Co)
90
Merck & Co plasmid pet-21d
(A) Three-dimensional structure of bovine RPE65 (PDB ID: 3FSN ). The crystallographically <t>unresolved</t> (aa107–125) region of RPE65 protein, modeled using I-TASSER server, is shown in red color. (B) Alignment of RPE65 aa107–125 from several jawed and jawless vertebrate taxa showing sequence conservation throughout evolution. (C) Helical wheel representation of the crystallographically unresolved aa107–125 region of RPE65 protein using HELIQUEST server. (D) Predicted secondary structure, relative accessibility and hydropathy analysis of the aa107–125 region of RPE65 protein using I-TASSER and ENDScript server, respectively. (E) Hydropathic moment plot of combined 35 different RPE65 aa107–125 and paralogous conserved region sequences from vertebrate and non-vertebrate carotenoid cleavage dioxygenase (CCD) paralogs, derived from HeliQuest . Red stars, RPE65 s; magenta circles, vertebrate BCO1s; blue circles, vertebrate BCO2s; black circle, early chordate ( Ciona ) CCD; yellow circles, arthropod ( Trinorchestia , Palaemon , Drosophila , and Galleria ) CCDs; and green circles, nematode ( Caenorhabditis ) -CCDs. Note tight grouping of all 10 RPE65 s distinct from other paralogs. (F) Physicochemical properties of the crystallographically unresolved (aa107–125) of RPE65 protein. Abbreviations: acc, accessibility; hyd, hydrophobicity.
Plasmid Pet 21d, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plasmid pet-21d/product/Merck & Co
Average 90 stars, based on 1 article reviews
plasmid pet-21d - by Bioz Stars, 2026-04
90/100 stars
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pet21d coledes1 imdes1 plasmid 107108  (Addgene inc)
N/A
Standard format Plasmid sent in bacteria as agar stab
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pet21d coledes2 imdes2 plasmid 107109  (Addgene inc)
N/A
Standard format Plasmid sent in bacteria as agar stab
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pet21d coledes16 imdes16 plasmid 107124  (Addgene inc)
N/A
Standard format Plasmid sent in bacteria as agar stab
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pet21d snord78 probe plasmid 73072  (Addgene inc)
N/A
Standard format Plasmid sent in bacteria as agar stab
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pet21d coledes9 imdes9 plasmid 107117  (Addgene inc)
N/A
Standard format Plasmid sent in bacteria as agar stab
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Image Search Results


(A) Three-dimensional structure of bovine RPE65 (PDB ID: 3FSN ). The crystallographically unresolved (aa107–125) region of RPE65 protein, modeled using I-TASSER server, is shown in red color. (B) Alignment of RPE65 aa107–125 from several jawed and jawless vertebrate taxa showing sequence conservation throughout evolution. (C) Helical wheel representation of the crystallographically unresolved aa107–125 region of RPE65 protein using HELIQUEST server. (D) Predicted secondary structure, relative accessibility and hydropathy analysis of the aa107–125 region of RPE65 protein using I-TASSER and ENDScript server, respectively. (E) Hydropathic moment plot of combined 35 different RPE65 aa107–125 and paralogous conserved region sequences from vertebrate and non-vertebrate carotenoid cleavage dioxygenase (CCD) paralogs, derived from HeliQuest . Red stars, RPE65 s; magenta circles, vertebrate BCO1s; blue circles, vertebrate BCO2s; black circle, early chordate ( Ciona ) CCD; yellow circles, arthropod ( Trinorchestia , Palaemon , Drosophila , and Galleria ) CCDs; and green circles, nematode ( Caenorhabditis ) -CCDs. Note tight grouping of all 10 RPE65 s distinct from other paralogs. (F) Physicochemical properties of the crystallographically unresolved (aa107–125) of RPE65 protein. Abbreviations: acc, accessibility; hyd, hydrophobicity.

Journal: Life Science Alliance

Article Title: An inducible amphipathic α-helix mediates subcellular targeting and membrane binding of RPE65

doi: 10.26508/lsa.202201546

Figure Lengend Snippet: (A) Three-dimensional structure of bovine RPE65 (PDB ID: 3FSN ). The crystallographically unresolved (aa107–125) region of RPE65 protein, modeled using I-TASSER server, is shown in red color. (B) Alignment of RPE65 aa107–125 from several jawed and jawless vertebrate taxa showing sequence conservation throughout evolution. (C) Helical wheel representation of the crystallographically unresolved aa107–125 region of RPE65 protein using HELIQUEST server. (D) Predicted secondary structure, relative accessibility and hydropathy analysis of the aa107–125 region of RPE65 protein using I-TASSER and ENDScript server, respectively. (E) Hydropathic moment plot of combined 35 different RPE65 aa107–125 and paralogous conserved region sequences from vertebrate and non-vertebrate carotenoid cleavage dioxygenase (CCD) paralogs, derived from HeliQuest . Red stars, RPE65 s; magenta circles, vertebrate BCO1s; blue circles, vertebrate BCO2s; black circle, early chordate ( Ciona ) CCD; yellow circles, arthropod ( Trinorchestia , Palaemon , Drosophila , and Galleria ) CCDs; and green circles, nematode ( Caenorhabditis ) -CCDs. Note tight grouping of all 10 RPE65 s distinct from other paralogs. (F) Physicochemical properties of the crystallographically unresolved (aa107–125) of RPE65 protein. Abbreviations: acc, accessibility; hyd, hydrophobicity.

Article Snippet: We cloned the crystallographically unresolved region (107–125 aa, AH 107-125 ) of RPE65 protein into the MBP fusion cloning vector pET His6 MBP TEV LIC (2M-T; a gift from Scott Gradia, plasmid # 29708; Addgene).

Techniques: Sequencing, Derivative Assay