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Image Search Results
Journal: The Journal of investigative dermatology
Article Title: Cathelicidin antimicrobial peptide LL-37 in psoriasis enables keratinocyte reactivity against TLR9 ligands
doi: 10.1038/jid.2011.259
Figure Lengend Snippet: ( a ) NHEKs were transfected with pUNO-mcs or pUNO-hTLR9-HA using Amaxa Human Keratinocyte Nucleofector Kit. 24 h after the transfection TLR9 mRNA expression were examined. ( b – d ) 24 h after the transfection with pUNO-mcs or pUNO-hTLR9-HA, NHEKs were stimulated with CpG alone (2 μM) ( b ) or LL-37 (10 μM) then CpG (2 μM) (c), genomic DNA (10 μg/ml) ( d ) for 24 h. The expression of IFNB1 mRNA was analyzed by RT-qPCR. *: P < 0.05, **: P < 0.01. Data are mean ± SEM of triplicate samples and representative of two independent experiments.
Article Snippet: Transfection was performed by Amaxa
Techniques: Transfection, Expressing, Quantitative RT-PCR