Journal: Communications Biology

Article Title: High-resolution structural and functional retinal imaging in the awake behaving mouse

doi: 10.1038/s42003-023-04896-x

Figure Lengend Snippet: a Head-restrained preparation for awake, behaving mouse retinal imaging. A surgical implanted Y-shape headplate on the mouse cranium restrains head motion, while ambulation is allowed on a rotational cylinder during imaging. b Schematic showing the position of the headplate on the center of the mouse cranium in parallel with the anterior-posterior axis. c A photograph of the head-restrained mouse imaged with commercial SLO. d Multimodal cSLO image of the retina in the head-restrained awake mice. Different retinal structures are visualized (Left - right): reflectance and autofluorescent imaging reveal major superficial retinal blood vessels and nerve fiber bundles under both NIR (top) and blue (bottom) channels. Fluorescent co-registration of NIR excited indocyanine green angiography (ICGA) of superficial blood vessel networks (rendered as red pseudo color) with blue excited fluorescence-labeled immune cells (rendered as green pseudo color) in a transgenic CX3CR1 mouse. ICGA of deep choroidal blood vessel network. e OCT cube and a representative b-scanned cross-section imaged in a head-restrained awake mouse. f AOSLO images show multimodal capabilities. Left: confocal reflectance image reveals nerve fiber bundle and retinal capillaries. Middle: phase-contrast imaging of a microcyst and a branch of retinal blood vessels. Right: fluorescence image of a YFP-labeled retinal ganglion cell with dendrites and axons highlighted.

Article Snippet: SLO and OCT imaging were performed with a commercial multimodal retinal imaging platform (HRA Spectralis, Heidelberg Engineering, Heidelberg, Germany).

Techniques: Imaging, Fluorescence, Labeling, Transgenic Assay

Journal: Theranostics

Article Title: Diagnosis of LVAD Thrombus using a High-Avidity Fibrin-Specific 99m Tc Probe

doi: 10.7150/thno.20271

Figure Lengend Snippet: Targeted fibrin binding of 99m Tc-F4A to carotid thrombus was compared to an irrelevantly targeted 99m Tc-I4A probe (~75 µCi/animal). A The signal from the clot-bearing carotid (%ID/g) was compared to the clot-free contralateral vessel response. 99m Tc-F4A binding to carotid thrombus was much greater than uptake in the clot-free contralateral carotid vessel (p<0.05). 99m Tc-I4A bound poorly to carotid thrombus and nuclear probe uptake did not differ from the nonspecific signal obtained in the clot-free carotid. Binding of the fibrin-specific 99m Tc-F4A to carotid clot was markedly greater (p=0.01) than the nonspecific binding of 99m Tc-I4A to thrombus. *p<0.05. B Representative maximum intensity projection image with a color map (arbitrary units) from 1 of 5 mice administered 99m Tc-F4A (40 µCi) by tail vein injection then imaged 2 h later with a Multispectral FX multimodal imaging system (Bruker-Biospin, Billerica, MA). A Marked single source signal originating from the right carotid thrombus was noted. All animals (n=5) displayed a strong in vivo carotid nuclear signal following 99m Tc-F4A.

Article Snippet: In a separate cohort of mice bearing carotid thrombus, 99m Tc-F4A was administered by tail vein injection and mice were imaged using Multispectral FX multimodal imaging system (Bruker-Biospin, Billerica, MA) after 2 h. Figure B provides a representative in vivo image showing robust signal (arbitrary units) in the targeted carotid with negligible contrast in the uninjured contralateral vessel.

Techniques: Binding Assay, Injection, Imaging, In Vivo