methanolic solutions Search Results


methanol  (Revvity)
95
Revvity methanol
Methanol, supplied by Revvity, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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concentrated hcl  (Chem Impex International)
95
Chem Impex International concentrated hcl
Concentrated Hcl, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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n ethylmaleimide  (Chem Impex International)
95
Chem Impex International n ethylmaleimide
N Ethylmaleimide, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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palmitic acid  (Chem Impex International)
95
Chem Impex International palmitic acid
Palmitic Acid, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chloramphenicol  (Teknova)
94
Teknova chloramphenicol
a , Wild-type beta-lactamase gene in plasmid pUC57, conferring ampicillin/carbenicillin resistance. Black arrows locate all wild-type serine codons, and blue arrows highlight TCA/TCG (TCR) codons. b , Top: Schematic representation of the transformation and selection process performed with all plasmids to study the escape rate of Ec_Syn61Δ3. Bottom left: Engineered genetic code of Ec_Syn61Δ3. Missing codons, anticodons, and genes are marked with dashed lines. Remaining codons, anticodons, and genes are marked with bold lines. Bottom right: Schema of central dogma of biology including our observation. c , Relative escape rate [(cfu/mL)/(cfu/mL)] defined as the number of colonies formed by Ec_Syn61Δ3 with (+) and without (-) the wild-type plasmid pUC57 relative to those of parallel experiments with pUC57-dTCR upon selection onto carbenicillin-containing agar plates ( n = 4 ). Escapees of Ec_Syn61Δ3 without the pUC57 plasmid were not detected (ND) onto carbenicillin-containing agar plates with up to 1 mL of OD 600 plated (Methods). The dashed bar length represents the ratio of 10 -9 cfu/mL and measured number of cells of Ec_Syn61Δ3 with pUC57-dTCR ( n = 4 ). d , Number of colonies formed by Ec_Syn61Δ3 escapees with selected plasmids including TCR-containing antibiotic resistance genes upon selection onto antibiotic-containing agar plates (mean ± standard deviation (SD), n = 2-4 ). The table below describes the number of TCR codons, plasmid copy number, presence of a his-tag on the antibiotic resistance protein, and antibiotic resistance for each plasmid. High or low plasmid copy number is denoted by + or -. Presence or absence of a His-tag is denoted by + or -. Antibiotic resistance acronyms: ChlR, <t>chloramphenicol;</t> KanR, kanamycin; AmpR/CarbR, ampicillin/carbenicillin; SpecR, spectinomycin; and GentR, gentamicin. Designs tagged as “max” or “min” contain TCR codons in serine positions with the highest or lowest probability for serine, respectively, based on the protein language model ESM-1b (Supplementary Fig. 3). Antibiotic acronyms: A, chloramphenicol; B, kanamycin; C, carbenicillin; D, spectinomycin; and E, gentamicin.
Chloramphenicol, supplied by Teknova, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rink amide polystyrene resin  (Chem Impex International)
95
Chem Impex International rink amide polystyrene resin
a , Wild-type beta-lactamase gene in plasmid pUC57, conferring ampicillin/carbenicillin resistance. Black arrows locate all wild-type serine codons, and blue arrows highlight TCA/TCG (TCR) codons. b , Top: Schematic representation of the transformation and selection process performed with all plasmids to study the escape rate of Ec_Syn61Δ3. Bottom left: Engineered genetic code of Ec_Syn61Δ3. Missing codons, anticodons, and genes are marked with dashed lines. Remaining codons, anticodons, and genes are marked with bold lines. Bottom right: Schema of central dogma of biology including our observation. c , Relative escape rate [(cfu/mL)/(cfu/mL)] defined as the number of colonies formed by Ec_Syn61Δ3 with (+) and without (-) the wild-type plasmid pUC57 relative to those of parallel experiments with pUC57-dTCR upon selection onto carbenicillin-containing agar plates ( n = 4 ). Escapees of Ec_Syn61Δ3 without the pUC57 plasmid were not detected (ND) onto carbenicillin-containing agar plates with up to 1 mL of OD 600 plated (Methods). The dashed bar length represents the ratio of 10 -9 cfu/mL and measured number of cells of Ec_Syn61Δ3 with pUC57-dTCR ( n = 4 ). d , Number of colonies formed by Ec_Syn61Δ3 escapees with selected plasmids including TCR-containing antibiotic resistance genes upon selection onto antibiotic-containing agar plates (mean ± standard deviation (SD), n = 2-4 ). The table below describes the number of TCR codons, plasmid copy number, presence of a his-tag on the antibiotic resistance protein, and antibiotic resistance for each plasmid. High or low plasmid copy number is denoted by + or -. Presence or absence of a His-tag is denoted by + or -. Antibiotic resistance acronyms: ChlR, <t>chloramphenicol;</t> KanR, kanamycin; AmpR/CarbR, ampicillin/carbenicillin; SpecR, spectinomycin; and GentR, gentamicin. Designs tagged as “max” or “min” contain TCR codons in serine positions with the highest or lowest probability for serine, respectively, based on the protein language model ESM-1b (Supplementary Fig. 3). Antibiotic acronyms: A, chloramphenicol; B, kanamycin; C, carbenicillin; D, spectinomycin; and E, gentamicin.
Rink Amide Polystyrene Resin, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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urea  (Chem Impex International)
95
Chem Impex International urea
a , Wild-type beta-lactamase gene in plasmid pUC57, conferring ampicillin/carbenicillin resistance. Black arrows locate all wild-type serine codons, and blue arrows highlight TCA/TCG (TCR) codons. b , Top: Schematic representation of the transformation and selection process performed with all plasmids to study the escape rate of Ec_Syn61Δ3. Bottom left: Engineered genetic code of Ec_Syn61Δ3. Missing codons, anticodons, and genes are marked with dashed lines. Remaining codons, anticodons, and genes are marked with bold lines. Bottom right: Schema of central dogma of biology including our observation. c , Relative escape rate [(cfu/mL)/(cfu/mL)] defined as the number of colonies formed by Ec_Syn61Δ3 with (+) and without (-) the wild-type plasmid pUC57 relative to those of parallel experiments with pUC57-dTCR upon selection onto carbenicillin-containing agar plates ( n = 4 ). Escapees of Ec_Syn61Δ3 without the pUC57 plasmid were not detected (ND) onto carbenicillin-containing agar plates with up to 1 mL of OD 600 plated (Methods). The dashed bar length represents the ratio of 10 -9 cfu/mL and measured number of cells of Ec_Syn61Δ3 with pUC57-dTCR ( n = 4 ). d , Number of colonies formed by Ec_Syn61Δ3 escapees with selected plasmids including TCR-containing antibiotic resistance genes upon selection onto antibiotic-containing agar plates (mean ± standard deviation (SD), n = 2-4 ). The table below describes the number of TCR codons, plasmid copy number, presence of a his-tag on the antibiotic resistance protein, and antibiotic resistance for each plasmid. High or low plasmid copy number is denoted by + or -. Presence or absence of a His-tag is denoted by + or -. Antibiotic resistance acronyms: ChlR, <t>chloramphenicol;</t> KanR, kanamycin; AmpR/CarbR, ampicillin/carbenicillin; SpecR, spectinomycin; and GentR, gentamicin. Designs tagged as “max” or “min” contain TCR codons in serine positions with the highest or lowest probability for serine, respectively, based on the protein language model ESM-1b (Supplementary Fig. 3). Antibiotic acronyms: A, chloramphenicol; B, kanamycin; C, carbenicillin; D, spectinomycin; and E, gentamicin.
Urea, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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oa national research council canada pure standard solution crm oa d  (National Research Council Canada)
93
National Research Council Canada oa national research council canada pure standard solution crm oa d
a , Wild-type beta-lactamase gene in plasmid pUC57, conferring ampicillin/carbenicillin resistance. Black arrows locate all wild-type serine codons, and blue arrows highlight TCA/TCG (TCR) codons. b , Top: Schematic representation of the transformation and selection process performed with all plasmids to study the escape rate of Ec_Syn61Δ3. Bottom left: Engineered genetic code of Ec_Syn61Δ3. Missing codons, anticodons, and genes are marked with dashed lines. Remaining codons, anticodons, and genes are marked with bold lines. Bottom right: Schema of central dogma of biology including our observation. c , Relative escape rate [(cfu/mL)/(cfu/mL)] defined as the number of colonies formed by Ec_Syn61Δ3 with (+) and without (-) the wild-type plasmid pUC57 relative to those of parallel experiments with pUC57-dTCR upon selection onto carbenicillin-containing agar plates ( n = 4 ). Escapees of Ec_Syn61Δ3 without the pUC57 plasmid were not detected (ND) onto carbenicillin-containing agar plates with up to 1 mL of OD 600 plated (Methods). The dashed bar length represents the ratio of 10 -9 cfu/mL and measured number of cells of Ec_Syn61Δ3 with pUC57-dTCR ( n = 4 ). d , Number of colonies formed by Ec_Syn61Δ3 escapees with selected plasmids including TCR-containing antibiotic resistance genes upon selection onto antibiotic-containing agar plates (mean ± standard deviation (SD), n = 2-4 ). The table below describes the number of TCR codons, plasmid copy number, presence of a his-tag on the antibiotic resistance protein, and antibiotic resistance for each plasmid. High or low plasmid copy number is denoted by + or -. Presence or absence of a His-tag is denoted by + or -. Antibiotic resistance acronyms: ChlR, <t>chloramphenicol;</t> KanR, kanamycin; AmpR/CarbR, ampicillin/carbenicillin; SpecR, spectinomycin; and GentR, gentamicin. Designs tagged as “max” or “min” contain TCR codons in serine positions with the highest or lowest probability for serine, respectively, based on the protein language model ESM-1b (Supplementary Fig. 3). Antibiotic acronyms: A, chloramphenicol; B, kanamycin; C, carbenicillin; D, spectinomycin; and E, gentamicin.
Oa National Research Council Canada Pure Standard Solution Crm Oa D, supplied by National Research Council Canada, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hydroxylamine wang resin  (Chem Impex International)
95
Chem Impex International hydroxylamine wang resin
a , Wild-type beta-lactamase gene in plasmid pUC57, conferring ampicillin/carbenicillin resistance. Black arrows locate all wild-type serine codons, and blue arrows highlight TCA/TCG (TCR) codons. b , Top: Schematic representation of the transformation and selection process performed with all plasmids to study the escape rate of Ec_Syn61Δ3. Bottom left: Engineered genetic code of Ec_Syn61Δ3. Missing codons, anticodons, and genes are marked with dashed lines. Remaining codons, anticodons, and genes are marked with bold lines. Bottom right: Schema of central dogma of biology including our observation. c , Relative escape rate [(cfu/mL)/(cfu/mL)] defined as the number of colonies formed by Ec_Syn61Δ3 with (+) and without (-) the wild-type plasmid pUC57 relative to those of parallel experiments with pUC57-dTCR upon selection onto carbenicillin-containing agar plates ( n = 4 ). Escapees of Ec_Syn61Δ3 without the pUC57 plasmid were not detected (ND) onto carbenicillin-containing agar plates with up to 1 mL of OD 600 plated (Methods). The dashed bar length represents the ratio of 10 -9 cfu/mL and measured number of cells of Ec_Syn61Δ3 with pUC57-dTCR ( n = 4 ). d , Number of colonies formed by Ec_Syn61Δ3 escapees with selected plasmids including TCR-containing antibiotic resistance genes upon selection onto antibiotic-containing agar plates (mean ± standard deviation (SD), n = 2-4 ). The table below describes the number of TCR codons, plasmid copy number, presence of a his-tag on the antibiotic resistance protein, and antibiotic resistance for each plasmid. High or low plasmid copy number is denoted by + or -. Presence or absence of a His-tag is denoted by + or -. Antibiotic resistance acronyms: ChlR, <t>chloramphenicol;</t> KanR, kanamycin; AmpR/CarbR, ampicillin/carbenicillin; SpecR, spectinomycin; and GentR, gentamicin. Designs tagged as “max” or “min” contain TCR codons in serine positions with the highest or lowest probability for serine, respectively, based on the protein language model ESM-1b (Supplementary Fig. 3). Antibiotic acronyms: A, chloramphenicol; B, kanamycin; C, carbenicillin; D, spectinomycin; and E, gentamicin.
Hydroxylamine Wang Resin, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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desmethyl spirolide c  (Laboratorio CIFGA)
86
Laboratorio CIFGA desmethyl spirolide c
a , Wild-type beta-lactamase gene in plasmid pUC57, conferring ampicillin/carbenicillin resistance. Black arrows locate all wild-type serine codons, and blue arrows highlight TCA/TCG (TCR) codons. b , Top: Schematic representation of the transformation and selection process performed with all plasmids to study the escape rate of Ec_Syn61Δ3. Bottom left: Engineered genetic code of Ec_Syn61Δ3. Missing codons, anticodons, and genes are marked with dashed lines. Remaining codons, anticodons, and genes are marked with bold lines. Bottom right: Schema of central dogma of biology including our observation. c , Relative escape rate [(cfu/mL)/(cfu/mL)] defined as the number of colonies formed by Ec_Syn61Δ3 with (+) and without (-) the wild-type plasmid pUC57 relative to those of parallel experiments with pUC57-dTCR upon selection onto carbenicillin-containing agar plates ( n = 4 ). Escapees of Ec_Syn61Δ3 without the pUC57 plasmid were not detected (ND) onto carbenicillin-containing agar plates with up to 1 mL of OD 600 plated (Methods). The dashed bar length represents the ratio of 10 -9 cfu/mL and measured number of cells of Ec_Syn61Δ3 with pUC57-dTCR ( n = 4 ). d , Number of colonies formed by Ec_Syn61Δ3 escapees with selected plasmids including TCR-containing antibiotic resistance genes upon selection onto antibiotic-containing agar plates (mean ± standard deviation (SD), n = 2-4 ). The table below describes the number of TCR codons, plasmid copy number, presence of a his-tag on the antibiotic resistance protein, and antibiotic resistance for each plasmid. High or low plasmid copy number is denoted by + or -. Presence or absence of a His-tag is denoted by + or -. Antibiotic resistance acronyms: ChlR, <t>chloramphenicol;</t> KanR, kanamycin; AmpR/CarbR, ampicillin/carbenicillin; SpecR, spectinomycin; and GentR, gentamicin. Designs tagged as “max” or “min” contain TCR codons in serine positions with the highest or lowest probability for serine, respectively, based on the protein language model ESM-1b (Supplementary Fig. 3). Antibiotic acronyms: A, chloramphenicol; B, kanamycin; C, carbenicillin; D, spectinomycin; and E, gentamicin.
Desmethyl Spirolide C, supplied by Laboratorio CIFGA, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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δ9 thc  (Cerilliant Corporation)
94
Cerilliant Corporation δ9 thc
a , Wild-type beta-lactamase gene in plasmid pUC57, conferring ampicillin/carbenicillin resistance. Black arrows locate all wild-type serine codons, and blue arrows highlight TCA/TCG (TCR) codons. b , Top: Schematic representation of the transformation and selection process performed with all plasmids to study the escape rate of Ec_Syn61Δ3. Bottom left: Engineered genetic code of Ec_Syn61Δ3. Missing codons, anticodons, and genes are marked with dashed lines. Remaining codons, anticodons, and genes are marked with bold lines. Bottom right: Schema of central dogma of biology including our observation. c , Relative escape rate [(cfu/mL)/(cfu/mL)] defined as the number of colonies formed by Ec_Syn61Δ3 with (+) and without (-) the wild-type plasmid pUC57 relative to those of parallel experiments with pUC57-dTCR upon selection onto carbenicillin-containing agar plates ( n = 4 ). Escapees of Ec_Syn61Δ3 without the pUC57 plasmid were not detected (ND) onto carbenicillin-containing agar plates with up to 1 mL of OD 600 plated (Methods). The dashed bar length represents the ratio of 10 -9 cfu/mL and measured number of cells of Ec_Syn61Δ3 with pUC57-dTCR ( n = 4 ). d , Number of colonies formed by Ec_Syn61Δ3 escapees with selected plasmids including TCR-containing antibiotic resistance genes upon selection onto antibiotic-containing agar plates (mean ± standard deviation (SD), n = 2-4 ). The table below describes the number of TCR codons, plasmid copy number, presence of a his-tag on the antibiotic resistance protein, and antibiotic resistance for each plasmid. High or low plasmid copy number is denoted by + or -. Presence or absence of a His-tag is denoted by + or -. Antibiotic resistance acronyms: ChlR, <t>chloramphenicol;</t> KanR, kanamycin; AmpR/CarbR, ampicillin/carbenicillin; SpecR, spectinomycin; and GentR, gentamicin. Designs tagged as “max” or “min” contain TCR codons in serine positions with the highest or lowest probability for serine, respectively, based on the protein language model ESM-1b (Supplementary Fig. 3). Antibiotic acronyms: A, chloramphenicol; B, kanamycin; C, carbenicillin; D, spectinomycin; and E, gentamicin.
δ9 Thc, supplied by Cerilliant Corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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h2so4  (Aladdin Scientific Corporation)
94
Aladdin Scientific Corporation h2so4
a , Wild-type beta-lactamase gene in plasmid pUC57, conferring ampicillin/carbenicillin resistance. Black arrows locate all wild-type serine codons, and blue arrows highlight TCA/TCG (TCR) codons. b , Top: Schematic representation of the transformation and selection process performed with all plasmids to study the escape rate of Ec_Syn61Δ3. Bottom left: Engineered genetic code of Ec_Syn61Δ3. Missing codons, anticodons, and genes are marked with dashed lines. Remaining codons, anticodons, and genes are marked with bold lines. Bottom right: Schema of central dogma of biology including our observation. c , Relative escape rate [(cfu/mL)/(cfu/mL)] defined as the number of colonies formed by Ec_Syn61Δ3 with (+) and without (-) the wild-type plasmid pUC57 relative to those of parallel experiments with pUC57-dTCR upon selection onto carbenicillin-containing agar plates ( n = 4 ). Escapees of Ec_Syn61Δ3 without the pUC57 plasmid were not detected (ND) onto carbenicillin-containing agar plates with up to 1 mL of OD 600 plated (Methods). The dashed bar length represents the ratio of 10 -9 cfu/mL and measured number of cells of Ec_Syn61Δ3 with pUC57-dTCR ( n = 4 ). d , Number of colonies formed by Ec_Syn61Δ3 escapees with selected plasmids including TCR-containing antibiotic resistance genes upon selection onto antibiotic-containing agar plates (mean ± standard deviation (SD), n = 2-4 ). The table below describes the number of TCR codons, plasmid copy number, presence of a his-tag on the antibiotic resistance protein, and antibiotic resistance for each plasmid. High or low plasmid copy number is denoted by + or -. Presence or absence of a His-tag is denoted by + or -. Antibiotic resistance acronyms: ChlR, <t>chloramphenicol;</t> KanR, kanamycin; AmpR/CarbR, ampicillin/carbenicillin; SpecR, spectinomycin; and GentR, gentamicin. Designs tagged as “max” or “min” contain TCR codons in serine positions with the highest or lowest probability for serine, respectively, based on the protein language model ESM-1b (Supplementary Fig. 3). Antibiotic acronyms: A, chloramphenicol; B, kanamycin; C, carbenicillin; D, spectinomycin; and E, gentamicin.
H2so4, supplied by Aladdin Scientific Corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


a , Wild-type beta-lactamase gene in plasmid pUC57, conferring ampicillin/carbenicillin resistance. Black arrows locate all wild-type serine codons, and blue arrows highlight TCA/TCG (TCR) codons. b , Top: Schematic representation of the transformation and selection process performed with all plasmids to study the escape rate of Ec_Syn61Δ3. Bottom left: Engineered genetic code of Ec_Syn61Δ3. Missing codons, anticodons, and genes are marked with dashed lines. Remaining codons, anticodons, and genes are marked with bold lines. Bottom right: Schema of central dogma of biology including our observation. c , Relative escape rate [(cfu/mL)/(cfu/mL)] defined as the number of colonies formed by Ec_Syn61Δ3 with (+) and without (-) the wild-type plasmid pUC57 relative to those of parallel experiments with pUC57-dTCR upon selection onto carbenicillin-containing agar plates ( n = 4 ). Escapees of Ec_Syn61Δ3 without the pUC57 plasmid were not detected (ND) onto carbenicillin-containing agar plates with up to 1 mL of OD 600 plated (Methods). The dashed bar length represents the ratio of 10 -9 cfu/mL and measured number of cells of Ec_Syn61Δ3 with pUC57-dTCR ( n = 4 ). d , Number of colonies formed by Ec_Syn61Δ3 escapees with selected plasmids including TCR-containing antibiotic resistance genes upon selection onto antibiotic-containing agar plates (mean ± standard deviation (SD), n = 2-4 ). The table below describes the number of TCR codons, plasmid copy number, presence of a his-tag on the antibiotic resistance protein, and antibiotic resistance for each plasmid. High or low plasmid copy number is denoted by + or -. Presence or absence of a His-tag is denoted by + or -. Antibiotic resistance acronyms: ChlR, chloramphenicol; KanR, kanamycin; AmpR/CarbR, ampicillin/carbenicillin; SpecR, spectinomycin; and GentR, gentamicin. Designs tagged as “max” or “min” contain TCR codons in serine positions with the highest or lowest probability for serine, respectively, based on the protein language model ESM-1b (Supplementary Fig. 3). Antibiotic acronyms: A, chloramphenicol; B, kanamycin; C, carbenicillin; D, spectinomycin; and E, gentamicin.

Journal: bioRxiv

Article Title: Preventing escape and malfunction of recoded cells due to tRNA base changes

doi: 10.1101/2024.07.18.604179

Figure Lengend Snippet: a , Wild-type beta-lactamase gene in plasmid pUC57, conferring ampicillin/carbenicillin resistance. Black arrows locate all wild-type serine codons, and blue arrows highlight TCA/TCG (TCR) codons. b , Top: Schematic representation of the transformation and selection process performed with all plasmids to study the escape rate of Ec_Syn61Δ3. Bottom left: Engineered genetic code of Ec_Syn61Δ3. Missing codons, anticodons, and genes are marked with dashed lines. Remaining codons, anticodons, and genes are marked with bold lines. Bottom right: Schema of central dogma of biology including our observation. c , Relative escape rate [(cfu/mL)/(cfu/mL)] defined as the number of colonies formed by Ec_Syn61Δ3 with (+) and without (-) the wild-type plasmid pUC57 relative to those of parallel experiments with pUC57-dTCR upon selection onto carbenicillin-containing agar plates ( n = 4 ). Escapees of Ec_Syn61Δ3 without the pUC57 plasmid were not detected (ND) onto carbenicillin-containing agar plates with up to 1 mL of OD 600 plated (Methods). The dashed bar length represents the ratio of 10 -9 cfu/mL and measured number of cells of Ec_Syn61Δ3 with pUC57-dTCR ( n = 4 ). d , Number of colonies formed by Ec_Syn61Δ3 escapees with selected plasmids including TCR-containing antibiotic resistance genes upon selection onto antibiotic-containing agar plates (mean ± standard deviation (SD), n = 2-4 ). The table below describes the number of TCR codons, plasmid copy number, presence of a his-tag on the antibiotic resistance protein, and antibiotic resistance for each plasmid. High or low plasmid copy number is denoted by + or -. Presence or absence of a His-tag is denoted by + or -. Antibiotic resistance acronyms: ChlR, chloramphenicol; KanR, kanamycin; AmpR/CarbR, ampicillin/carbenicillin; SpecR, spectinomycin; and GentR, gentamicin. Designs tagged as “max” or “min” contain TCR codons in serine positions with the highest or lowest probability for serine, respectively, based on the protein language model ESM-1b (Supplementary Fig. 3). Antibiotic acronyms: A, chloramphenicol; B, kanamycin; C, carbenicillin; D, spectinomycin; and E, gentamicin.

Article Snippet: We worked with the following antibiotics and final concentrations: Chloramphenicol (C0316, Teknova) at 25 µg/mL, Kanamycin (K2199, Teknova) at 50 µg/mL, Carbenicillin (E0096, Teknova) at 100 µg/mL, Spectinomycin (S9525, Teknova) at 75 µg/mL, and Gentamicin (15750060, Thermo Fisher Scientific, Life Technologies) at 15 µg/mL.

Techniques: Plasmid Preparation, Transformation Assay, Selection, Standard Deviation