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Image Search Results
Journal: Clinical biochemistry
Article Title: Development of an Equilibrium Dialysis ID-UPLC-MS/MS Candidate Reference Measurement Procedure for Free Thyroxine in Human Serum
doi: 10.1016/j.clinbiochem.2023.03.010
Figure Lengend Snippet: Representative chromatograms of (A) neat sample (T4 concentration of 64.5 pmol/L, 2.6 pg on-column); (B) native serum dialysate sample (T4 concentration of 20.6 pmol/L, 2.4 pg on-column); and (C) TIC showing the separation of a) (T3), b) (rT3), and c) (T4) in neat sample (T4 concentration of 64.4 pmol/L, 2.5 pg on-column). (T3, rT3, and T4 in neat samples were prepared in 10% acetonitrile in water containing 0.1% (v/v) formic acid, and serum dialysate samples were extracted with C18 SPE followed by LLE. Neat samples and serum dialysate samples were analyzed by UPLC-MS/MS, as described in the method section.)
Article Snippet: Chromatographic separation was performed on a
Techniques: Concentration Assay, Tandem Mass Spectroscopy