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Image Search Results


Journal: iScience

Article Title: Reduction of IFN-I responses by plasmacytoid dendritic cells in a longitudinal trans men cohort

doi: 10.1016/j.isci.2023.108209

Figure Lengend Snippet:

Article Snippet: RNA was extracted with ethanol precipitation and treated with DNase I (catalog no. AM2222, Invitrogen/Thermo Fisher) as well as with a recombinant ribonuclease inhibitor (RNaseOUT, catalog no. 100000840, Invitrogen/Thermo Fisher) before reverse transcription into cDNA on a FlexCycler Block assembly T48 device (Analytik Jena, Jena, Germany), using the qScriber cDNA Synthesis Kit (catalog no. RTK0101, highQU, Kraichtal, Germany) following manufacturer’s recommendations. qPCR was performed with SybrGreen (catalog no. QPD0450, highQU) at a LightCycler 480 II-96 device (Roche, Basel, Switzerland) using the LightCycler480 software version 1.5.0.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Software