Journal: PLoS ONE

Article Title: Short and Long-Term Effects of hVEGF-A 165 in Cre-Activated Transgenic Mice

doi: 10.1371/journal.pone.0000013

Figure Lengend Snippet: A. Liver: Encapsulated hepatocellular carcinoma was highly necrotic and focally calcified (Haematoxylin-eosin). B. Lung: Papillary adenocarcinoma. (Haematoxylin-eosin). C. Macroscopical changes in liver (box shows the area of the microscopical sections in F–H). D. Macroscopical changes in spleen, control spleen on the left (microscopical sections in I–K). E. Macroscopical changes in paratubarian area (microscopical sections in L–N). F. Cavernous hemangioma featuring focal hyalinization. G. The same tumor mass area with higher magnification (haematoxylin-eosin). H. Weak focal hVEGF-A 165 immunoreactivity was present in the same area (True Blue as a chromogen), arrows indicate positive cells. I–K. Fibrous scars formed by collagenous and elastic fibres surrounded by dilated cystic spaces, focally filled with blood and revealing inconsistently CD34 positive lining. I. Hematoxylin-eosin. (an arrow points to an area shown in K) J. CD34 immunostaining. K. The same area as in I with higher magnification (stained with Masson Trichrom). Asterix in I and J indicate the same area. L. Cystically dilated paratubarian spaces with signs of old haemorrhage and thrombus formation (marked with *), an open circle points to an area which was highly vascularized as shown in M. (CD31 immunostaining) and a box indicates an area which revealed focal hVEGF-A 165 immunopositivity shown in N. (hVEGF-A 165 immunostaining). Bar : F, I, J - 500 µm, G, H, K - 100 µm, A, B, M, N - 200 µm L - 1000 µm.

Article Snippet: Tissue vascularization was assessed by immunostaining with CD34 antibody (HyCult biotechnology BV, clone MEC 14.7, dilution 1∶20).

Techniques: Immunostaining, Staining

Journal: Circulation. Cardiovascular Imaging

Article Title: Spin Labeling-MRI Detects Increased Myocardial Blood Flow Post Endothelial Cell Transplantation in the Infarcted Heart

doi: 10.1161/CIRCIMAGING.111.966317

Figure Lengend Snippet: Slice-average and regional MBF at 1-day (inset of A) and 2-weeks post-MI (A). The MBF maps and corresponding LGE images were shown for a representative heart from EC (B–C, arrows pointing to the phantom) and Vehicle group (D–E). Immunostaining for vWF from an EC- (F) or Vehicle-treated heart (G). Double immunostaining of CD34 which reacts only to human tissue and vWF antibody which reacts to rat and human antigen revealed incorporation of ECs into capillaries in the infarcted (H) and border (I) region. Scale bars = 100 μm. MVD (= the number of capillaries per field of 1.1 mm2). *: statistically significant comparing the EC vs. Vehicle group (A, J).

Article Snippet: Sections were immunostained using the following antibodies: 1. rabbit polyclonal anti- von Willebrand Factor (vWF) antibody (Sigma, St. Louis, MO) and FITC-conjugated goat anti-rabbit secondary antibody, 2. mouse monoclonal anti-human CD34 antibody (Abcam, Cambridge, MA) and Cy3-conjugated goat anti-mouse secondary antibody.

Techniques: Immunostaining, Double Immunostaining