cd45ro fitc Search Results


cd45ro  (Miltenyi Biotec)
93
Miltenyi Biotec cd45ro
Immunophenotyping panel for multiplexed tissue imaging of cancer.
Cd45ro, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd45ro/product/Miltenyi Biotec
Average 93 stars, based on 1 article reviews
cd45ro - by Bioz Stars, 2026-04
93/100 stars
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fitc anti cd45ro  (R&D Systems)
91
R&D Systems fitc anti cd45ro
Immunophenotyping panel for multiplexed tissue imaging of cancer.
Fitc Anti Cd45ro, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fitc anti cd45ro/product/R&D Systems
Average 91 stars, based on 1 article reviews
fitc anti cd45ro - by Bioz Stars, 2026-04
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cd45ro-pe clone uchl1 antibody  (Exbio Praha)
90
Exbio Praha cd45ro-pe clone uchl1 antibody
Representative flow cytometric analysis of a hemodialysis patient. Representative dot plots depicting lymphocyte gating with B-lymphocytes (CD19+), and T lymphocytes (CD3+), CD4+ T cells, CD8+ T cells, naïve (CD45RA+) and memory <t>(CD45RO+)</t> T cell isoforms, naïve (CD4+CD45RA) T helper cells and memory (CD4+CD45RO) T helper cells, NK cells (CD3-CD16+CD56+) and NKT cells (CD3+CD16+CD56+).
Cd45ro Pe Clone Uchl1 Antibody, supplied by Exbio Praha, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd45ro-pe clone uchl1 antibody/product/Exbio Praha
Average 90 stars, based on 1 article reviews
cd45ro-pe clone uchl1 antibody - by Bioz Stars, 2026-04
90/100 stars
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fitc-anti-cd45ro  (MBL International)
90
MBL International fitc-anti-cd45ro
Fig. 4. CD95 expression, susceptibility to apoptosis and CD95/ezrin co-localization in activated human primary T lymphocytes. (A) CD95 membrane expression and apoptosis were analyzed in electronically gated CD4+ by three-color immunofluorescence and FACS analysis in day 1- (upper panels) and day 6- (lower panels) activated human primary lymphocytes (PBL). Left panels are the CD95+/CD69+ CD4+ PBL; central panels are the <t>CD95+/CD45RO+</t> CD4+ lymphocytes; right panels are the apoptotic cells (FITC–annexin V+) CD4+ PBL after triggering with an anti-CD95 mAb (see Materials and methods). The results are representative of four experiments. (B) CD95 (green, FITC)/ezrin (red, TRITC) localization in day 1- (left panel) and day 6- (right panel) activated human primary T lymphocytes. In the upper and lower panels the single and double stainings are shown, respectively. Note the co-localization in day 6-activated lymphocytes, as revealed by the yellow staining (IVM) (magnification ×1000).
Fitc Anti Cd45ro, supplied by MBL International, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fitc-anti-cd45ro/product/MBL International
Average 90 stars, based on 1 article reviews
fitc-anti-cd45ro - by Bioz Stars, 2026-04
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cd45ro-fitc uchl1  (QuantoBio)
90
QuantoBio cd45ro-fitc uchl1
Fig. 4. CD95 expression, susceptibility to apoptosis and CD95/ezrin co-localization in activated human primary T lymphocytes. (A) CD95 membrane expression and apoptosis were analyzed in electronically gated CD4+ by three-color immunofluorescence and FACS analysis in day 1- (upper panels) and day 6- (lower panels) activated human primary lymphocytes (PBL). Left panels are the CD95+/CD69+ CD4+ PBL; central panels are the <t>CD95+/CD45RO+</t> CD4+ lymphocytes; right panels are the apoptotic cells (FITC–annexin V+) CD4+ PBL after triggering with an anti-CD95 mAb (see Materials and methods). The results are representative of four experiments. (B) CD95 (green, FITC)/ezrin (red, TRITC) localization in day 1- (left panel) and day 6- (right panel) activated human primary T lymphocytes. In the upper and lower panels the single and double stainings are shown, respectively. Note the co-localization in day 6-activated lymphocytes, as revealed by the yellow staining (IVM) (magnification ×1000).
Cd45ro Fitc Uchl1, supplied by QuantoBio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd45ro-fitc uchl1/product/QuantoBio
Average 90 stars, based on 1 article reviews
cd45ro-fitc uchl1 - by Bioz Stars, 2026-04
90/100 stars
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anti-cd45ro-fitc  (Proimmune)
90
Proimmune anti-cd45ro-fitc
Fig. 4. CD95 expression, susceptibility to apoptosis and CD95/ezrin co-localization in activated human primary T lymphocytes. (A) CD95 membrane expression and apoptosis were analyzed in electronically gated CD4+ by three-color immunofluorescence and FACS analysis in day 1- (upper panels) and day 6- (lower panels) activated human primary lymphocytes (PBL). Left panels are the CD95+/CD69+ CD4+ PBL; central panels are the <t>CD95+/CD45RO+</t> CD4+ lymphocytes; right panels are the apoptotic cells (FITC–annexin V+) CD4+ PBL after triggering with an anti-CD95 mAb (see Materials and methods). The results are representative of four experiments. (B) CD95 (green, FITC)/ezrin (red, TRITC) localization in day 1- (left panel) and day 6- (right panel) activated human primary T lymphocytes. In the upper and lower panels the single and double stainings are shown, respectively. Note the co-localization in day 6-activated lymphocytes, as revealed by the yellow staining (IVM) (magnification ×1000).
Anti Cd45ro Fitc, supplied by Proimmune, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-cd45ro-fitc/product/Proimmune
Average 90 stars, based on 1 article reviews
anti-cd45ro-fitc - by Bioz Stars, 2026-04
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cd45ro (uchl1 fitc  (Biozol Diagnostica Vertrieb GmbH)
90
Biozol Diagnostica Vertrieb GmbH cd45ro (uchl1 fitc
Fig. 4. CD95 expression, susceptibility to apoptosis and CD95/ezrin co-localization in activated human primary T lymphocytes. (A) CD95 membrane expression and apoptosis were analyzed in electronically gated CD4+ by three-color immunofluorescence and FACS analysis in day 1- (upper panels) and day 6- (lower panels) activated human primary lymphocytes (PBL). Left panels are the CD95+/CD69+ CD4+ PBL; central panels are the <t>CD95+/CD45RO+</t> CD4+ lymphocytes; right panels are the apoptotic cells (FITC–annexin V+) CD4+ PBL after triggering with an anti-CD95 mAb (see Materials and methods). The results are representative of four experiments. (B) CD95 (green, FITC)/ezrin (red, TRITC) localization in day 1- (left panel) and day 6- (right panel) activated human primary T lymphocytes. In the upper and lower panels the single and double stainings are shown, respectively. Note the co-localization in day 6-activated lymphocytes, as revealed by the yellow staining (IVM) (magnification ×1000).
Cd45ro (Uchl1 Fitc, supplied by Biozol Diagnostica Vertrieb GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd45ro (uchl1 fitc/product/Biozol Diagnostica Vertrieb GmbH
Average 90 stars, based on 1 article reviews
cd45ro (uchl1 fitc - by Bioz Stars, 2026-04
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vimentin antibody, anti-human, fitc, reafinity  (Miltenyi Biotec)
94
Miltenyi Biotec vimentin antibody, anti-human, fitc, reafinity
Fig. 4. CD95 expression, susceptibility to apoptosis and CD95/ezrin co-localization in activated human primary T lymphocytes. (A) CD95 membrane expression and apoptosis were analyzed in electronically gated CD4+ by three-color immunofluorescence and FACS analysis in day 1- (upper panels) and day 6- (lower panels) activated human primary lymphocytes (PBL). Left panels are the CD95+/CD69+ CD4+ PBL; central panels are the <t>CD95+/CD45RO+</t> CD4+ lymphocytes; right panels are the apoptotic cells (FITC–annexin V+) CD4+ PBL after triggering with an anti-CD95 mAb (see Materials and methods). The results are representative of four experiments. (B) CD95 (green, FITC)/ezrin (red, TRITC) localization in day 1- (left panel) and day 6- (right panel) activated human primary T lymphocytes. In the upper and lower panels the single and double stainings are shown, respectively. Note the co-localization in day 6-activated lymphocytes, as revealed by the yellow staining (IVM) (magnification ×1000).
Vimentin Antibody, Anti Human, Fitc, Reafinity, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/vimentin antibody, anti-human, fitc, reafinity/product/Miltenyi Biotec
Average 94 stars, based on 1 article reviews
vimentin antibody, anti-human, fitc, reafinity - by Bioz Stars, 2026-04
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cd45ro-fitc  (Ancell corporation)
90
Ancell corporation cd45ro-fitc
Fig. 4. CD95 expression, susceptibility to apoptosis and CD95/ezrin co-localization in activated human primary T lymphocytes. (A) CD95 membrane expression and apoptosis were analyzed in electronically gated CD4+ by three-color immunofluorescence and FACS analysis in day 1- (upper panels) and day 6- (lower panels) activated human primary lymphocytes (PBL). Left panels are the CD95+/CD69+ CD4+ PBL; central panels are the <t>CD95+/CD45RO+</t> CD4+ lymphocytes; right panels are the apoptotic cells (FITC–annexin V+) CD4+ PBL after triggering with an anti-CD95 mAb (see Materials and methods). The results are representative of four experiments. (B) CD95 (green, FITC)/ezrin (red, TRITC) localization in day 1- (left panel) and day 6- (right panel) activated human primary T lymphocytes. In the upper and lower panels the single and double stainings are shown, respectively. Note the co-localization in day 6-activated lymphocytes, as revealed by the yellow staining (IVM) (magnification ×1000).
Cd45ro Fitc, supplied by Ancell corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd45ro-fitc/product/Ancell corporation
Average 90 stars, based on 1 article reviews
cd45ro-fitc - by Bioz Stars, 2026-04
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cd45ro-fitc  (Caltag-Medsystems ltd)
90
Caltag-Medsystems ltd cd45ro-fitc
Fig. 4. CD95 expression, susceptibility to apoptosis and CD95/ezrin co-localization in activated human primary T lymphocytes. (A) CD95 membrane expression and apoptosis were analyzed in electronically gated CD4+ by three-color immunofluorescence and FACS analysis in day 1- (upper panels) and day 6- (lower panels) activated human primary lymphocytes (PBL). Left panels are the CD95+/CD69+ CD4+ PBL; central panels are the <t>CD95+/CD45RO+</t> CD4+ lymphocytes; right panels are the apoptotic cells (FITC–annexin V+) CD4+ PBL after triggering with an anti-CD95 mAb (see Materials and methods). The results are representative of four experiments. (B) CD95 (green, FITC)/ezrin (red, TRITC) localization in day 1- (left panel) and day 6- (right panel) activated human primary T lymphocytes. In the upper and lower panels the single and double stainings are shown, respectively. Note the co-localization in day 6-activated lymphocytes, as revealed by the yellow staining (IVM) (magnification ×1000).
Cd45ro Fitc, supplied by Caltag-Medsystems ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd45ro-fitc/product/Caltag-Medsystems ltd
Average 90 stars, based on 1 article reviews
cd45ro-fitc - by Bioz Stars, 2026-04
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cd20 cytoplasmic antibody, anti-human, fitc, reafinity  (Miltenyi Biotec)
93
Miltenyi Biotec cd20 cytoplasmic antibody, anti-human, fitc, reafinity
Fig. 4. CD95 expression, susceptibility to apoptosis and CD95/ezrin co-localization in activated human primary T lymphocytes. (A) CD95 membrane expression and apoptosis were analyzed in electronically gated CD4+ by three-color immunofluorescence and FACS analysis in day 1- (upper panels) and day 6- (lower panels) activated human primary lymphocytes (PBL). Left panels are the CD95+/CD69+ CD4+ PBL; central panels are the <t>CD95+/CD45RO+</t> CD4+ lymphocytes; right panels are the apoptotic cells (FITC–annexin V+) CD4+ PBL after triggering with an anti-CD95 mAb (see Materials and methods). The results are representative of four experiments. (B) CD95 (green, FITC)/ezrin (red, TRITC) localization in day 1- (left panel) and day 6- (right panel) activated human primary T lymphocytes. In the upper and lower panels the single and double stainings are shown, respectively. Note the co-localization in day 6-activated lymphocytes, as revealed by the yellow staining (IVM) (magnification ×1000).
Cd20 Cytoplasmic Antibody, Anti Human, Fitc, Reafinity, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd20 cytoplasmic antibody, anti-human, fitc, reafinity/product/Miltenyi Biotec
Average 93 stars, based on 1 article reviews
cd20 cytoplasmic antibody, anti-human, fitc, reafinity - by Bioz Stars, 2026-04
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anti human cd45ro monoclonal antibody fitc conjugated clone uchl1  (Diaclone)
N/A
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Image Search Results


Immunophenotyping panel for multiplexed tissue imaging of cancer.

Journal: Frontiers in Immunology

Article Title: Unveiling spatial complexity in solid tumor immune microenvironments through multiplexed imaging

doi: 10.3389/fimmu.2024.1383932

Figure Lengend Snippet: Immunophenotyping panel for multiplexed tissue imaging of cancer.

Article Snippet: CD45RO , UCHL1 , 50 , 130-113-549 , FITC , Miltenyi Biotec.

Techniques: Imaging

T cell subset classification and spatial distribution in an HCC sample. (A) Bar graph quantifications of gated T cell CD3 + T cells subpopulations (CD4 + T h , CD8 + T c , FoxP3 + /CD25 + T reg ) as well as T cell differentiation subsets (T N , T SCM , T CM , T EM , T EMRA , T TE ) for HCC tumor margin and tumor core. (B) Subclassification criteria used for T cell differentiation subset gating. Detailed gating scheme see <xref ref-type= Supplementary Figures S5A–D Spatial distribution of CD3+ T cell subsets as well as CD4+ and CD8+ differentiation subsets for tumor margin (C) and tumor core (D) . Arrowheads in (C, D) highlight the spatial distribution of regulatory T cells (T reg ). (E) Violin plots demonstrating expression levels of markers used for the definition of T cell differentiation subsets in tumor margin and tumor core: CD25, FoxP3, CD45RA, CD45RO, CD27, CD95, CD11a. Depicted markers and annotated cell types as indicated by the color code. ROI sizes: Tumor margin (ROI15) and tumor core (ROI16): 975 x 769 µm. " width="100%" height="100%">

Journal: Frontiers in Immunology

Article Title: Unveiling spatial complexity in solid tumor immune microenvironments through multiplexed imaging

doi: 10.3389/fimmu.2024.1383932

Figure Lengend Snippet: T cell subset classification and spatial distribution in an HCC sample. (A) Bar graph quantifications of gated T cell CD3 + T cells subpopulations (CD4 + T h , CD8 + T c , FoxP3 + /CD25 + T reg ) as well as T cell differentiation subsets (T N , T SCM , T CM , T EM , T EMRA , T TE ) for HCC tumor margin and tumor core. (B) Subclassification criteria used for T cell differentiation subset gating. Detailed gating scheme see Supplementary Figures S5A–D Spatial distribution of CD3+ T cell subsets as well as CD4+ and CD8+ differentiation subsets for tumor margin (C) and tumor core (D) . Arrowheads in (C, D) highlight the spatial distribution of regulatory T cells (T reg ). (E) Violin plots demonstrating expression levels of markers used for the definition of T cell differentiation subsets in tumor margin and tumor core: CD25, FoxP3, CD45RA, CD45RO, CD27, CD95, CD11a. Depicted markers and annotated cell types as indicated by the color code. ROI sizes: Tumor margin (ROI15) and tumor core (ROI16): 975 x 769 µm.

Article Snippet: CD45RO , UCHL1 , 50 , 130-113-549 , FITC , Miltenyi Biotec.

Techniques: Cell Differentiation, Expressing

Representative flow cytometric analysis of a hemodialysis patient. Representative dot plots depicting lymphocyte gating with B-lymphocytes (CD19+), and T lymphocytes (CD3+), CD4+ T cells, CD8+ T cells, naïve (CD45RA+) and memory (CD45RO+) T cell isoforms, naïve (CD4+CD45RA) T helper cells and memory (CD4+CD45RO) T helper cells, NK cells (CD3-CD16+CD56+) and NKT cells (CD3+CD16+CD56+).

Journal: Frontiers in Immunology

Article Title: The Humoral Immune Response to BNT162b2 Vaccine Is Associated With Circulating CD19+ B Lymphocytes and the Naïve CD45RA to Memory CD45RO CD4+ T Helper Cells Ratio in Hemodialysis Patients and Kidney Transplant Recipients

doi: 10.3389/fimmu.2021.760249

Figure Lengend Snippet: Representative flow cytometric analysis of a hemodialysis patient. Representative dot plots depicting lymphocyte gating with B-lymphocytes (CD19+), and T lymphocytes (CD3+), CD4+ T cells, CD8+ T cells, naïve (CD45RA+) and memory (CD45RO+) T cell isoforms, naïve (CD4+CD45RA) T helper cells and memory (CD4+CD45RO) T helper cells, NK cells (CD3-CD16+CD56+) and NKT cells (CD3+CD16+CD56+).

Article Snippet: The particular anti-human antibodies used were: CD3-FITC (Clone UCHT1), CD4-PE (Clone MEM-241), CD4-APC (Clone MEM-241), CD8-APC (Clone MEM-31), CD16-PE (Clone MEM-154), CD19-APC (Clone LT19), CD45-PerCP (Clone MEM-28), CD45RA-FITC (Clone MEM-56), CD45RO-PE (Clone UCHL1) and CD56-PE (Clone LT56), purchased from EXBIO, Praha SA.

Techniques:

Scatter plots representing individual values for each patient of the fraction of CD4+CD45RA/CD4+CD45RO T-helper cells and the percentage of CD19+ B cells respectively at baseline (T0), immediately before the second vaccine dose (T1) and 2 weeks after administration of the second dose (T2) in hemodialysis patients and kidney transplant recipients. HD, hemodialysis patients; KTRs, kidney transplant recipients.

Journal: Frontiers in Immunology

Article Title: The Humoral Immune Response to BNT162b2 Vaccine Is Associated With Circulating CD19+ B Lymphocytes and the Naïve CD45RA to Memory CD45RO CD4+ T Helper Cells Ratio in Hemodialysis Patients and Kidney Transplant Recipients

doi: 10.3389/fimmu.2021.760249

Figure Lengend Snippet: Scatter plots representing individual values for each patient of the fraction of CD4+CD45RA/CD4+CD45RO T-helper cells and the percentage of CD19+ B cells respectively at baseline (T0), immediately before the second vaccine dose (T1) and 2 weeks after administration of the second dose (T2) in hemodialysis patients and kidney transplant recipients. HD, hemodialysis patients; KTRs, kidney transplant recipients.

Article Snippet: The particular anti-human antibodies used were: CD3-FITC (Clone UCHT1), CD4-PE (Clone MEM-241), CD4-APC (Clone MEM-241), CD8-APC (Clone MEM-31), CD16-PE (Clone MEM-154), CD19-APC (Clone LT19), CD45-PerCP (Clone MEM-28), CD45RA-FITC (Clone MEM-56), CD45RO-PE (Clone UCHL1) and CD56-PE (Clone LT56), purchased from EXBIO, Praha SA.

Techniques:

Correlation between the humoral immune response with different lymphocyte subsets. The fraction of CD4+CD45RA/CD4+CD45RO (X-axis) has been plotted against the percentage of CD19+ cells (Y-axis) in kidney transplant recipients (circular points) and hemodialysis patients (square points) patients. The antibody counts following the first (0-100 grey, 101-500 green, 501-1500 yellow, >1500 red) and the second (different sizes from small to large from 0-30000) dose of vaccine were also depicted in each point. The results are presented for T0, T1 and T2 respectively. HD, hemodialysis patients; KTRs, kidney transplant recipients; HD, hemodialysis patients; KTRs, kidney transplant recipients.

Journal: Frontiers in Immunology

Article Title: The Humoral Immune Response to BNT162b2 Vaccine Is Associated With Circulating CD19+ B Lymphocytes and the Naïve CD45RA to Memory CD45RO CD4+ T Helper Cells Ratio in Hemodialysis Patients and Kidney Transplant Recipients

doi: 10.3389/fimmu.2021.760249

Figure Lengend Snippet: Correlation between the humoral immune response with different lymphocyte subsets. The fraction of CD4+CD45RA/CD4+CD45RO (X-axis) has been plotted against the percentage of CD19+ cells (Y-axis) in kidney transplant recipients (circular points) and hemodialysis patients (square points) patients. The antibody counts following the first (0-100 grey, 101-500 green, 501-1500 yellow, >1500 red) and the second (different sizes from small to large from 0-30000) dose of vaccine were also depicted in each point. The results are presented for T0, T1 and T2 respectively. HD, hemodialysis patients; KTRs, kidney transplant recipients; HD, hemodialysis patients; KTRs, kidney transplant recipients.

Article Snippet: The particular anti-human antibodies used were: CD3-FITC (Clone UCHT1), CD4-PE (Clone MEM-241), CD4-APC (Clone MEM-241), CD8-APC (Clone MEM-31), CD16-PE (Clone MEM-154), CD19-APC (Clone LT19), CD45-PerCP (Clone MEM-28), CD45RA-FITC (Clone MEM-56), CD45RO-PE (Clone UCHL1) and CD56-PE (Clone LT56), purchased from EXBIO, Praha SA.

Techniques:

Fig. 4. CD95 expression, susceptibility to apoptosis and CD95/ezrin co-localization in activated human primary T lymphocytes. (A) CD95 membrane expression and apoptosis were analyzed in electronically gated CD4+ by three-color immunofluorescence and FACS analysis in day 1- (upper panels) and day 6- (lower panels) activated human primary lymphocytes (PBL). Left panels are the CD95+/CD69+ CD4+ PBL; central panels are the CD95+/CD45RO+ CD4+ lymphocytes; right panels are the apoptotic cells (FITC–annexin V+) CD4+ PBL after triggering with an anti-CD95 mAb (see Materials and methods). The results are representative of four experiments. (B) CD95 (green, FITC)/ezrin (red, TRITC) localization in day 1- (left panel) and day 6- (right panel) activated human primary T lymphocytes. In the upper and lower panels the single and double stainings are shown, respectively. Note the co-localization in day 6-activated lymphocytes, as revealed by the yellow staining (IVM) (magnification ×1000).

Journal:

Article Title: CD95 (APO-1/Fas) linkage to the actin cytoskeleton through ezrin in human T lymphocytes: a novel regulatory mechanism of the CD95 apoptotic pathway

doi: 10.1093/emboj/19.19.5123

Figure Lengend Snippet: Fig. 4. CD95 expression, susceptibility to apoptosis and CD95/ezrin co-localization in activated human primary T lymphocytes. (A) CD95 membrane expression and apoptosis were analyzed in electronically gated CD4+ by three-color immunofluorescence and FACS analysis in day 1- (upper panels) and day 6- (lower panels) activated human primary lymphocytes (PBL). Left panels are the CD95+/CD69+ CD4+ PBL; central panels are the CD95+/CD45RO+ CD4+ lymphocytes; right panels are the apoptotic cells (FITC–annexin V+) CD4+ PBL after triggering with an anti-CD95 mAb (see Materials and methods). The results are representative of four experiments. (B) CD95 (green, FITC)/ezrin (red, TRITC) localization in day 1- (left panel) and day 6- (right panel) activated human primary T lymphocytes. In the upper and lower panels the single and double stainings are shown, respectively. Note the co-localization in day 6-activated lymphocytes, as revealed by the yellow staining (IVM) (magnification ×1000).

Article Snippet: After this time cells were incubated at 4°C with saturating concentration of directly conjugated: (i) FITC-anti-CD69, PE-anti-CD95 (MBL International Corporation, Watertown, USA) PerCP-anti-CD4; (ii) FITC-anti-CD45RO, PE-anti-CD95, PerCP-anti-CD4; and (iii) isotype matched normal FITC, PE and PerCP Ab (Becton Dickinson, Mountain View, CA).

Techniques: Expressing, Immunofluorescence, Staining