cd34 a700 bd Search Results


mouse integrin alpha 7 alexa fluor® 700-conjugated antibody  (Bio-Techne corporation)
94
Bio-Techne corporation mouse integrin alpha 7 alexa fluor® 700-conjugated antibody
Mouse Integrin Alpha 7 Alexa Fluor® 700 Conjugated Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse integrin alpha 7 alexa fluor® 700-conjugated antibody/product/Bio-Techne corporation
Average 94 stars, based on 1 article reviews
mouse integrin alpha 7 alexa fluor® 700-conjugated antibody - by Bioz Stars, 2026-04
94/100 stars
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cd34-a647  (Becton Dickinson)
90
Becton Dickinson cd34-a647
Induced deletion of the miR17~92 cluster causes a loss of haematopoietic stem and <t>progenitor</t> <t>cells</t> (HSPCs). C57BL/6 wt mice (Ly5.1+) were lethally irradiated and reconstituted with a 1:1 mixture of E14.5 foetal liver cells from UBC-GFP embryos (competitor cells) and E14.5 foetal liver cells from miR17~92deldel, miR17~92+/del or wt embryos (test cells). At 8–10 weeks after transplantation, the fraction of reconstituted test cells was determined by FACS analysis gating on Ly5.1+GFP− cells (donor test cells). Representative FACS plots for the gating strategies are provided for wt mice. Percentages of GFP-negative cells (test cells) were determined for the indicated cell populations. Data represent mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (Student's t test miR17~92deldel, miR17~92+/del and wt). a LSK (lineage−c-KIT+SCA-1+) cells and progenitor cells (lineage−c-KIT+SCA-1−) were identified in the lineage marker negative compartment (TER119−, CD4−, CD8a−, Ly6G−, B220−, CD2−, CD3−, CD19−, F4/80−, NK1.1−, GR-1−) by staining with antibodies against c-KIT and SCA-1. b Oligopotent progenitors, including common myeloid progenitors (CMP), granulocyte/macrophage progenitors (GMP) and megakaryocyte/erythroid progenitors, were identified by cell surface staining for the SLAM markers (CD16/32, CD150, <t>CD34)</t> in the progenitor cell population (lineage−c-KIT+SCA-1−). Common lymphoid progenitors (CLP) were identified as c-KITmedSCA-1medIL-7 receptor+ within the lineage marker negative cell populations. c Long-term haematopoietic stem cells (LT-HSC), short-term haematopoietic stem cells (ST-HSC) as well as multipotent progenitor cells (MPP) were identified using the FLK series markers <t>(CD34,</t> CD135) within the LSK cell population. d Haematopoietic stem cells (HSC), multipotent progenitor cells (MPP) and haematopoietic progenitor cells (HPC-1/2) were identified using cell surface staining for the SLAM markers (CD48, CD150) within the LSK cell population. n = number of donor/number of recipient mice as indicated
Cd34 A647, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd34-a647/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
cd34-a647 - by Bioz Stars, 2026-04
90/100 stars
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cd127-apcef780  (Thermo Fisher)
90
Thermo Fisher cd127-apcef780
Induced deletion of the miR17~92 cluster causes a loss of haematopoietic stem and <t>progenitor</t> <t>cells</t> (HSPCs). C57BL/6 wt mice (Ly5.1+) were lethally irradiated and reconstituted with a 1:1 mixture of E14.5 foetal liver cells from UBC-GFP embryos (competitor cells) and E14.5 foetal liver cells from miR17~92deldel, miR17~92+/del or wt embryos (test cells). At 8–10 weeks after transplantation, the fraction of reconstituted test cells was determined by FACS analysis gating on Ly5.1+GFP− cells (donor test cells). Representative FACS plots for the gating strategies are provided for wt mice. Percentages of GFP-negative cells (test cells) were determined for the indicated cell populations. Data represent mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (Student's t test miR17~92deldel, miR17~92+/del and wt). a LSK (lineage−c-KIT+SCA-1+) cells and progenitor cells (lineage−c-KIT+SCA-1−) were identified in the lineage marker negative compartment (TER119−, CD4−, CD8a−, Ly6G−, B220−, CD2−, CD3−, CD19−, F4/80−, NK1.1−, GR-1−) by staining with antibodies against c-KIT and SCA-1. b Oligopotent progenitors, including common myeloid progenitors (CMP), granulocyte/macrophage progenitors (GMP) and megakaryocyte/erythroid progenitors, were identified by cell surface staining for the SLAM markers (CD16/32, CD150, <t>CD34)</t> in the progenitor cell population (lineage−c-KIT+SCA-1−). Common lymphoid progenitors (CLP) were identified as c-KITmedSCA-1medIL-7 receptor+ within the lineage marker negative cell populations. c Long-term haematopoietic stem cells (LT-HSC), short-term haematopoietic stem cells (ST-HSC) as well as multipotent progenitor cells (MPP) were identified using the FLK series markers <t>(CD34,</t> CD135) within the LSK cell population. d Haematopoietic stem cells (HSC), multipotent progenitor cells (MPP) and haematopoietic progenitor cells (HPC-1/2) were identified using cell surface staining for the SLAM markers (CD48, CD150) within the LSK cell population. n = number of donor/number of recipient mice as indicated
Cd127 Apcef780, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd127-apcef780/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
cd127-apcef780 - by Bioz Stars, 2026-04
90/100 stars
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cd16/32-percpcy5.5  (Becton Dickinson)
90
Becton Dickinson cd16/32-percpcy5.5
Induced deletion of the miR17~92 cluster causes a loss of haematopoietic stem and progenitor cells (HSPCs). C57BL/6 wt mice (Ly5.1+) were lethally irradiated and reconstituted with a 1:1 mixture of E14.5 foetal liver cells from UBC-GFP embryos (competitor cells) and E14.5 foetal liver cells from miR17~92deldel, miR17~92+/del or wt embryos (test cells). At 8–10 weeks after transplantation, the fraction of reconstituted test cells was determined by FACS analysis gating on Ly5.1+GFP− cells (donor test cells). Representative FACS plots for the gating strategies are provided for wt mice. Percentages of GFP-negative cells (test cells) were determined for the indicated cell populations. Data represent mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (Student's t test miR17~92deldel, miR17~92+/del and wt). a LSK (lineage−c-KIT+SCA-1+) cells and progenitor cells (lineage−c-KIT+SCA-1−) were identified in the lineage marker negative compartment (TER119−, CD4−, CD8a−, Ly6G−, B220−, CD2−, CD3−, CD19−, F4/80−, NK1.1−, GR-1−) by staining with antibodies against c-KIT and SCA-1. b Oligopotent progenitors, including common myeloid progenitors (CMP), granulocyte/macrophage progenitors (GMP) and megakaryocyte/erythroid progenitors, were identified by cell surface staining for the SLAM <t>markers</t> <t>(CD16/32,</t> CD150, CD34) in the progenitor cell population (lineage−c-KIT+SCA-1−). Common lymphoid progenitors (CLP) were identified as c-KITmedSCA-1medIL-7 receptor+ within the lineage marker negative cell populations. c Long-term haematopoietic stem cells (LT-HSC), short-term haematopoietic stem cells (ST-HSC) as well as multipotent progenitor cells (MPP) were identified using the FLK series markers (CD34, CD135) within the LSK cell population. d Haematopoietic stem cells (HSC), multipotent progenitor cells (MPP) and haematopoietic progenitor cells (HPC-1/2) were identified using cell surface staining for the SLAM markers (CD48, CD150) within the LSK cell population. n = number of donor/number of recipient mice as indicated
Cd16/32 Percpcy5.5, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd16/32-percpcy5.5/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
cd16/32-percpcy5.5 - by Bioz Stars, 2026-04
90/100 stars
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cd135-pe  (Thermo Fisher)
90
Thermo Fisher cd135-pe
Induced deletion of the miR17~92 cluster causes a loss of haematopoietic stem and progenitor cells (HSPCs). C57BL/6 wt mice (Ly5.1+) were lethally irradiated and reconstituted with a 1:1 mixture of E14.5 foetal liver cells from UBC-GFP embryos (competitor cells) and E14.5 foetal liver cells from miR17~92deldel, miR17~92+/del or wt embryos (test cells). At 8–10 weeks after transplantation, the fraction of reconstituted test cells was determined by FACS analysis gating on Ly5.1+GFP− cells (donor test cells). Representative FACS plots for the gating strategies are provided for wt mice. Percentages of GFP-negative cells (test cells) were determined for the indicated cell populations. Data represent mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (Student's t test miR17~92deldel, miR17~92+/del and wt). a LSK (lineage−c-KIT+SCA-1+) cells and progenitor cells (lineage−c-KIT+SCA-1−) were identified in the lineage marker negative compartment (TER119−, CD4−, CD8a−, Ly6G−, B220−, CD2−, CD3−, CD19−, F4/80−, NK1.1−, GR-1−) by staining with antibodies against c-KIT and SCA-1. b Oligopotent progenitors, including common myeloid progenitors (CMP), granulocyte/macrophage progenitors (GMP) and megakaryocyte/erythroid progenitors, were identified by cell surface staining for the SLAM <t>markers</t> <t>(CD16/32,</t> CD150, CD34) in the progenitor cell population (lineage−c-KIT+SCA-1−). Common lymphoid progenitors (CLP) were identified as c-KITmedSCA-1medIL-7 receptor+ within the lineage marker negative cell populations. c Long-term haematopoietic stem cells (LT-HSC), short-term haematopoietic stem cells (ST-HSC) as well as multipotent progenitor cells (MPP) were identified using the FLK series markers (CD34, CD135) within the LSK cell population. d Haematopoietic stem cells (HSC), multipotent progenitor cells (MPP) and haematopoietic progenitor cells (HPC-1/2) were identified using cell surface staining for the SLAM markers (CD48, CD150) within the LSK cell population. n = number of donor/number of recipient mice as indicated
Cd135 Pe, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd135-pe/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
cd135-pe - by Bioz Stars, 2026-04
90/100 stars
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cd105-pe mj718  (Thermo Fisher)
90
Thermo Fisher cd105-pe mj718
Induced deletion of the miR17~92 cluster causes a loss of haematopoietic stem and progenitor cells (HSPCs). C57BL/6 wt mice (Ly5.1+) were lethally irradiated and reconstituted with a 1:1 mixture of E14.5 foetal liver cells from UBC-GFP embryos (competitor cells) and E14.5 foetal liver cells from miR17~92deldel, miR17~92+/del or wt embryos (test cells). At 8–10 weeks after transplantation, the fraction of reconstituted test cells was determined by FACS analysis gating on Ly5.1+GFP− cells (donor test cells). Representative FACS plots for the gating strategies are provided for wt mice. Percentages of GFP-negative cells (test cells) were determined for the indicated cell populations. Data represent mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (Student's t test miR17~92deldel, miR17~92+/del and wt). a LSK (lineage−c-KIT+SCA-1+) cells and progenitor cells (lineage−c-KIT+SCA-1−) were identified in the lineage marker negative compartment (TER119−, CD4−, CD8a−, Ly6G−, B220−, CD2−, CD3−, CD19−, F4/80−, NK1.1−, GR-1−) by staining with antibodies against c-KIT and SCA-1. b Oligopotent progenitors, including common myeloid progenitors (CMP), granulocyte/macrophage progenitors (GMP) and megakaryocyte/erythroid progenitors, were identified by cell surface staining for the SLAM <t>markers</t> <t>(CD16/32,</t> CD150, CD34) in the progenitor cell population (lineage−c-KIT+SCA-1−). Common lymphoid progenitors (CLP) were identified as c-KITmedSCA-1medIL-7 receptor+ within the lineage marker negative cell populations. c Long-term haematopoietic stem cells (LT-HSC), short-term haematopoietic stem cells (ST-HSC) as well as multipotent progenitor cells (MPP) were identified using the FLK series markers (CD34, CD135) within the LSK cell population. d Haematopoietic stem cells (HSC), multipotent progenitor cells (MPP) and haematopoietic progenitor cells (HPC-1/2) were identified using cell surface staining for the SLAM markers (CD48, CD150) within the LSK cell population. n = number of donor/number of recipient mice as indicated
Cd105 Pe Mj718, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd105-pe mj718/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
cd105-pe mj718 - by Bioz Stars, 2026-04
90/100 stars
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cd127-apc a7r34  (Thermo Fisher)
90
Thermo Fisher cd127-apc a7r34
Induced deletion of the miR17~92 cluster causes a loss of haematopoietic stem and progenitor cells (HSPCs). C57BL/6 wt mice (Ly5.1+) were lethally irradiated and reconstituted with a 1:1 mixture of E14.5 foetal liver cells from UBC-GFP embryos (competitor cells) and E14.5 foetal liver cells from miR17~92deldel, miR17~92+/del or wt embryos (test cells). At 8–10 weeks after transplantation, the fraction of reconstituted test cells was determined by FACS analysis gating on Ly5.1+GFP− cells (donor test cells). Representative FACS plots for the gating strategies are provided for wt mice. Percentages of GFP-negative cells (test cells) were determined for the indicated cell populations. Data represent mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (Student's t test miR17~92deldel, miR17~92+/del and wt). a LSK (lineage−c-KIT+SCA-1+) cells and progenitor cells (lineage−c-KIT+SCA-1−) were identified in the lineage marker negative compartment (TER119−, CD4−, CD8a−, Ly6G−, B220−, CD2−, CD3−, CD19−, F4/80−, NK1.1−, GR-1−) by staining with antibodies against c-KIT and SCA-1. b Oligopotent progenitors, including common myeloid progenitors (CMP), granulocyte/macrophage progenitors (GMP) and megakaryocyte/erythroid progenitors, were identified by cell surface staining for the SLAM <t>markers</t> <t>(CD16/32,</t> CD150, CD34) in the progenitor cell population (lineage−c-KIT+SCA-1−). Common lymphoid progenitors (CLP) were identified as c-KITmedSCA-1medIL-7 receptor+ within the lineage marker negative cell populations. c Long-term haematopoietic stem cells (LT-HSC), short-term haematopoietic stem cells (ST-HSC) as well as multipotent progenitor cells (MPP) were identified using the FLK series markers (CD34, CD135) within the LSK cell population. d Haematopoietic stem cells (HSC), multipotent progenitor cells (MPP) and haematopoietic progenitor cells (HPC-1/2) were identified using cell surface staining for the SLAM markers (CD48, CD150) within the LSK cell population. n = number of donor/number of recipient mice as indicated
Cd127 Apc A7r34, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd127-apc a7r34/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
cd127-apc a7r34 - by Bioz Stars, 2026-04
90/100 stars
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cd48-pecy7 hm48-1  (Thermo Fisher)
90
Thermo Fisher cd48-pecy7 hm48-1
Induced deletion of the miR17~92 cluster causes a loss of haematopoietic stem and progenitor cells (HSPCs). C57BL/6 wt mice (Ly5.1+) were lethally irradiated and reconstituted with a 1:1 mixture of E14.5 foetal liver cells from UBC-GFP embryos (competitor cells) and E14.5 foetal liver cells from miR17~92deldel, miR17~92+/del or wt embryos (test cells). At 8–10 weeks after transplantation, the fraction of reconstituted test cells was determined by FACS analysis gating on Ly5.1+GFP− cells (donor test cells). Representative FACS plots for the gating strategies are provided for wt mice. Percentages of GFP-negative cells (test cells) were determined for the indicated cell populations. Data represent mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (Student's t test miR17~92deldel, miR17~92+/del and wt). a LSK (lineage−c-KIT+SCA-1+) cells and progenitor cells (lineage−c-KIT+SCA-1−) were identified in the lineage marker negative compartment (TER119−, CD4−, CD8a−, Ly6G−, B220−, CD2−, CD3−, CD19−, F4/80−, NK1.1−, GR-1−) by staining with antibodies against c-KIT and SCA-1. b Oligopotent progenitors, including common myeloid progenitors (CMP), granulocyte/macrophage progenitors (GMP) and megakaryocyte/erythroid progenitors, were identified by cell surface staining for the SLAM <t>markers</t> <t>(CD16/32,</t> CD150, CD34) in the progenitor cell population (lineage−c-KIT+SCA-1−). Common lymphoid progenitors (CLP) were identified as c-KITmedSCA-1medIL-7 receptor+ within the lineage marker negative cell populations. c Long-term haematopoietic stem cells (LT-HSC), short-term haematopoietic stem cells (ST-HSC) as well as multipotent progenitor cells (MPP) were identified using the FLK series markers (CD34, CD135) within the LSK cell population. d Haematopoietic stem cells (HSC), multipotent progenitor cells (MPP) and haematopoietic progenitor cells (HPC-1/2) were identified using cell surface staining for the SLAM markers (CD48, CD150) within the LSK cell population. n = number of donor/number of recipient mice as indicated
Cd48 Pecy7 Hm48 1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd48-pecy7 hm48-1/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
cd48-pecy7 hm48-1 - by Bioz Stars, 2026-04
90/100 stars
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cd41-pecy7  (Becton Dickinson)
90
Becton Dickinson cd41-pecy7
Induced deletion of the miR17~92 cluster causes a loss of haematopoietic stem and progenitor cells (HSPCs). C57BL/6 wt mice (Ly5.1+) were lethally irradiated and reconstituted with a 1:1 mixture of E14.5 foetal liver cells from UBC-GFP embryos (competitor cells) and E14.5 foetal liver cells from miR17~92deldel, miR17~92+/del or wt embryos (test cells). At 8–10 weeks after transplantation, the fraction of reconstituted test cells was determined by FACS analysis gating on Ly5.1+GFP− cells (donor test cells). Representative FACS plots for the gating strategies are provided for wt mice. Percentages of GFP-negative cells (test cells) were determined for the indicated cell populations. Data represent mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (Student's t test miR17~92deldel, miR17~92+/del and wt). a LSK (lineage−c-KIT+SCA-1+) cells and progenitor cells (lineage−c-KIT+SCA-1−) were identified in the lineage marker negative compartment (TER119−, CD4−, CD8a−, Ly6G−, B220−, CD2−, CD3−, CD19−, F4/80−, NK1.1−, GR-1−) by staining with antibodies against c-KIT and SCA-1. b Oligopotent progenitors, including common myeloid progenitors (CMP), granulocyte/macrophage progenitors (GMP) and megakaryocyte/erythroid progenitors, were identified by cell surface staining for the SLAM <t>markers</t> <t>(CD16/32,</t> CD150, CD34) in the progenitor cell population (lineage−c-KIT+SCA-1−). Common lymphoid progenitors (CLP) were identified as c-KITmedSCA-1medIL-7 receptor+ within the lineage marker negative cell populations. c Long-term haematopoietic stem cells (LT-HSC), short-term haematopoietic stem cells (ST-HSC) as well as multipotent progenitor cells (MPP) were identified using the FLK series markers (CD34, CD135) within the LSK cell population. d Haematopoietic stem cells (HSC), multipotent progenitor cells (MPP) and haematopoietic progenitor cells (HPC-1/2) were identified using cell surface staining for the SLAM markers (CD48, CD150) within the LSK cell population. n = number of donor/number of recipient mice as indicated
Cd41 Pecy7, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd41-pecy7/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
cd41-pecy7 - by Bioz Stars, 2026-04
90/100 stars
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cd117-bv711  (Becton Dickinson)
90
Becton Dickinson cd117-bv711
Induced deletion of the miR17~92 cluster causes a loss of haematopoietic stem and progenitor cells (HSPCs). C57BL/6 wt mice (Ly5.1+) were lethally irradiated and reconstituted with a 1:1 mixture of E14.5 foetal liver cells from UBC-GFP embryos (competitor cells) and E14.5 foetal liver cells from miR17~92deldel, miR17~92+/del or wt embryos (test cells). At 8–10 weeks after transplantation, the fraction of reconstituted test cells was determined by FACS analysis gating on Ly5.1+GFP− cells (donor test cells). Representative FACS plots for the gating strategies are provided for wt mice. Percentages of GFP-negative cells (test cells) were determined for the indicated cell populations. Data represent mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (Student's t test miR17~92deldel, miR17~92+/del and wt). a LSK (lineage−c-KIT+SCA-1+) cells and progenitor cells (lineage−c-KIT+SCA-1−) were identified in the lineage marker negative compartment (TER119−, CD4−, CD8a−, Ly6G−, B220−, CD2−, CD3−, CD19−, F4/80−, NK1.1−, GR-1−) by staining with antibodies against c-KIT and SCA-1. b Oligopotent progenitors, including common myeloid progenitors (CMP), granulocyte/macrophage progenitors (GMP) and megakaryocyte/erythroid progenitors, were identified by cell surface staining for the SLAM <t>markers</t> <t>(CD16/32,</t> CD150, CD34) in the progenitor cell population (lineage−c-KIT+SCA-1−). Common lymphoid progenitors (CLP) were identified as c-KITmedSCA-1medIL-7 receptor+ within the lineage marker negative cell populations. c Long-term haematopoietic stem cells (LT-HSC), short-term haematopoietic stem cells (ST-HSC) as well as multipotent progenitor cells (MPP) were identified using the FLK series markers (CD34, CD135) within the LSK cell population. d Haematopoietic stem cells (HSC), multipotent progenitor cells (MPP) and haematopoietic progenitor cells (HPC-1/2) were identified using cell surface staining for the SLAM markers (CD48, CD150) within the LSK cell population. n = number of donor/number of recipient mice as indicated
Cd117 Bv711, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd117-bv711/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
cd117-bv711 - by Bioz Stars, 2026-04
90/100 stars
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anti cd34 a700  (Thermo Fisher)
86
Thermo Fisher anti cd34 a700
Induced deletion of the miR17~92 cluster causes a loss of haematopoietic stem and progenitor cells (HSPCs). C57BL/6 wt mice (Ly5.1+) were lethally irradiated and reconstituted with a 1:1 mixture of E14.5 foetal liver cells from UBC-GFP embryos (competitor cells) and E14.5 foetal liver cells from miR17~92deldel, miR17~92+/del or wt embryos (test cells). At 8–10 weeks after transplantation, the fraction of reconstituted test cells was determined by FACS analysis gating on Ly5.1+GFP− cells (donor test cells). Representative FACS plots for the gating strategies are provided for wt mice. Percentages of GFP-negative cells (test cells) were determined for the indicated cell populations. Data represent mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (Student's t test miR17~92deldel, miR17~92+/del and wt). a LSK (lineage−c-KIT+SCA-1+) cells and progenitor cells (lineage−c-KIT+SCA-1−) were identified in the lineage marker negative compartment (TER119−, CD4−, CD8a−, Ly6G−, B220−, CD2−, CD3−, CD19−, F4/80−, NK1.1−, GR-1−) by staining with antibodies against c-KIT and SCA-1. b Oligopotent progenitors, including common myeloid progenitors (CMP), granulocyte/macrophage progenitors (GMP) and megakaryocyte/erythroid progenitors, were identified by cell surface staining for the SLAM <t>markers</t> <t>(CD16/32,</t> CD150, CD34) in the progenitor cell population (lineage−c-KIT+SCA-1−). Common lymphoid progenitors (CLP) were identified as c-KITmedSCA-1medIL-7 receptor+ within the lineage marker negative cell populations. c Long-term haematopoietic stem cells (LT-HSC), short-term haematopoietic stem cells (ST-HSC) as well as multipotent progenitor cells (MPP) were identified using the FLK series markers (CD34, CD135) within the LSK cell population. d Haematopoietic stem cells (HSC), multipotent progenitor cells (MPP) and haematopoietic progenitor cells (HPC-1/2) were identified using cell surface staining for the SLAM markers (CD48, CD150) within the LSK cell population. n = number of donor/number of recipient mice as indicated
Anti Cd34 A700, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti cd34 a700/product/Thermo Fisher
Average 86 stars, based on 1 article reviews
anti cd34 a700 - by Bioz Stars, 2026-04
86/100 stars
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cd16 32  (Thermo Fisher)
86
Thermo Fisher cd16 32
Induced deletion of the miR17~92 cluster causes a loss of haematopoietic stem and progenitor cells (HSPCs). C57BL/6 wt mice (Ly5.1+) were lethally irradiated and reconstituted with a 1:1 mixture of E14.5 foetal liver cells from UBC-GFP embryos (competitor cells) and E14.5 foetal liver cells from miR17~92deldel, miR17~92+/del or wt embryos (test cells). At 8–10 weeks after transplantation, the fraction of reconstituted test cells was determined by FACS analysis gating on Ly5.1+GFP− cells (donor test cells). Representative FACS plots for the gating strategies are provided for wt mice. Percentages of GFP-negative cells (test cells) were determined for the indicated cell populations. Data represent mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (Student's t test miR17~92deldel, miR17~92+/del and wt). a LSK (lineage−c-KIT+SCA-1+) cells and progenitor cells (lineage−c-KIT+SCA-1−) were identified in the lineage marker negative compartment (TER119−, CD4−, CD8a−, Ly6G−, B220−, CD2−, CD3−, CD19−, F4/80−, NK1.1−, GR-1−) by staining with antibodies against c-KIT and SCA-1. b Oligopotent progenitors, including common myeloid progenitors (CMP), granulocyte/macrophage progenitors (GMP) and megakaryocyte/erythroid progenitors, were identified by cell surface staining for the SLAM <t>markers</t> <t>(CD16/32,</t> CD150, CD34) in the progenitor cell population (lineage−c-KIT+SCA-1−). Common lymphoid progenitors (CLP) were identified as c-KITmedSCA-1medIL-7 receptor+ within the lineage marker negative cell populations. c Long-term haematopoietic stem cells (LT-HSC), short-term haematopoietic stem cells (ST-HSC) as well as multipotent progenitor cells (MPP) were identified using the FLK series markers (CD34, CD135) within the LSK cell population. d Haematopoietic stem cells (HSC), multipotent progenitor cells (MPP) and haematopoietic progenitor cells (HPC-1/2) were identified using cell surface staining for the SLAM markers (CD48, CD150) within the LSK cell population. n = number of donor/number of recipient mice as indicated
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Induced deletion of the miR17~92 cluster causes a loss of haematopoietic stem and progenitor cells (HSPCs). C57BL/6 wt mice (Ly5.1+) were lethally irradiated and reconstituted with a 1:1 mixture of E14.5 foetal liver cells from UBC-GFP embryos (competitor cells) and E14.5 foetal liver cells from miR17~92deldel, miR17~92+/del or wt embryos (test cells). At 8–10 weeks after transplantation, the fraction of reconstituted test cells was determined by FACS analysis gating on Ly5.1+GFP− cells (donor test cells). Representative FACS plots for the gating strategies are provided for wt mice. Percentages of GFP-negative cells (test cells) were determined for the indicated cell populations. Data represent mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (Student's t test miR17~92deldel, miR17~92+/del and wt). a LSK (lineage−c-KIT+SCA-1+) cells and progenitor cells (lineage−c-KIT+SCA-1−) were identified in the lineage marker negative compartment (TER119−, CD4−, CD8a−, Ly6G−, B220−, CD2−, CD3−, CD19−, F4/80−, NK1.1−, GR-1−) by staining with antibodies against c-KIT and SCA-1. b Oligopotent progenitors, including common myeloid progenitors (CMP), granulocyte/macrophage progenitors (GMP) and megakaryocyte/erythroid progenitors, were identified by cell surface staining for the SLAM markers (CD16/32, CD150, CD34) in the progenitor cell population (lineage−c-KIT+SCA-1−). Common lymphoid progenitors (CLP) were identified as c-KITmedSCA-1medIL-7 receptor+ within the lineage marker negative cell populations. c Long-term haematopoietic stem cells (LT-HSC), short-term haematopoietic stem cells (ST-HSC) as well as multipotent progenitor cells (MPP) were identified using the FLK series markers (CD34, CD135) within the LSK cell population. d Haematopoietic stem cells (HSC), multipotent progenitor cells (MPP) and haematopoietic progenitor cells (HPC-1/2) were identified using cell surface staining for the SLAM markers (CD48, CD150) within the LSK cell population. n = number of donor/number of recipient mice as indicated

Journal: Cell Death and Differentiation

Article Title: miR17~92 restrains pro-apoptotic BIM to ensure survival of haematopoietic stem and progenitor cells

doi: 10.1038/s41418-019-0430-6

Figure Lengend Snippet: Induced deletion of the miR17~92 cluster causes a loss of haematopoietic stem and progenitor cells (HSPCs). C57BL/6 wt mice (Ly5.1+) were lethally irradiated and reconstituted with a 1:1 mixture of E14.5 foetal liver cells from UBC-GFP embryos (competitor cells) and E14.5 foetal liver cells from miR17~92deldel, miR17~92+/del or wt embryos (test cells). At 8–10 weeks after transplantation, the fraction of reconstituted test cells was determined by FACS analysis gating on Ly5.1+GFP− cells (donor test cells). Representative FACS plots for the gating strategies are provided for wt mice. Percentages of GFP-negative cells (test cells) were determined for the indicated cell populations. Data represent mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (Student's t test miR17~92deldel, miR17~92+/del and wt). a LSK (lineage−c-KIT+SCA-1+) cells and progenitor cells (lineage−c-KIT+SCA-1−) were identified in the lineage marker negative compartment (TER119−, CD4−, CD8a−, Ly6G−, B220−, CD2−, CD3−, CD19−, F4/80−, NK1.1−, GR-1−) by staining with antibodies against c-KIT and SCA-1. b Oligopotent progenitors, including common myeloid progenitors (CMP), granulocyte/macrophage progenitors (GMP) and megakaryocyte/erythroid progenitors, were identified by cell surface staining for the SLAM markers (CD16/32, CD150, CD34) in the progenitor cell population (lineage−c-KIT+SCA-1−). Common lymphoid progenitors (CLP) were identified as c-KITmedSCA-1medIL-7 receptor+ within the lineage marker negative cell populations. c Long-term haematopoietic stem cells (LT-HSC), short-term haematopoietic stem cells (ST-HSC) as well as multipotent progenitor cells (MPP) were identified using the FLK series markers (CD34, CD135) within the LSK cell population. d Haematopoietic stem cells (HSC), multipotent progenitor cells (MPP) and haematopoietic progenitor cells (HPC-1/2) were identified using cell surface staining for the SLAM markers (CD48, CD150) within the LSK cell population. n = number of donor/number of recipient mice as indicated

Article Snippet: HSPCs were analysed by staining with fluorochrome-conjugated monoclonal antibodies against the following cell surface markers: CD150-BV421 (clone TC15-12F12.2, Biolegend), CD127-APC (IL-7 receptor, clone A7R34, eBioscience), CD48-PECy7 (clone HM48-1, eBioscience), CD105-PE (clone MJ718, eBioscience), CD34-A647 (clone RAM34, BD), CD117-BV711 (clone 2B8, BD), CD16/32-PerCPCy5.5 (clone 2.4G2, BD), CD135-PE (eBioscience), CD41-PECy7 (clone MWReg30, BD), SCA-1-A595, CD127-APCCy7 (eBioscience), CD9-A647, CD16_32-FITC, CD41-PECy7, CD2-A700, CD4-A700, CD8-A700, GR-1-A700, F4/80-A700, CD19-A700, B220-A700, Ly6G-A700, TER-119-A700 and NK1.1-A700 [ 27 ].

Techniques: Irradiation, Transplantation Assay, Marker, Staining

The reduction of haematopoietic stem and progenitor cells (HSPCs) caused by the induced deletion of miR17~92 can be fully prevented by the loss of pro-apoptotic BIM. a–c C57BL/6 wt mice (Ly5.1+) were lethally irradiated and reconstituted with a 1:1 mixture of bone marrow cells from UBC-GFP mice (competitor cells) and bone marrow cells from mice of the indicated genotypes (test cells). Reconstituted mice were treated with tamoxifen 8–15 weeks post-transplantation to activate the CreERT2 recombinase. After an additional 8–10 weeks the haematopoietic stem/progenitor cell populations (HSPC) indicated were identified by staining with antibodies against cell type specific surface markers. Representative FACS plots for the gating strategies are provided for wt mice in Fig. ​Fig.3.3. Percentages of GFP-negative cells (test cells) were determined for each cell subset. Data represent mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (Student's t test miR17~92fl/fl; RosaCreERT2+/Ki vs. wt, RosaCreERT2+/Ki, miR17~92fl/fl; RosaCreERT2+/Ki; Bim−/−, Bim−/−, miR17~92fl/fl; RosaCreERT2+/Ki; Puma−/− and Puma−/− mice). n = number of donor/number of recipient mice as indicated

Journal: Cell Death and Differentiation

Article Title: miR17~92 restrains pro-apoptotic BIM to ensure survival of haematopoietic stem and progenitor cells

doi: 10.1038/s41418-019-0430-6

Figure Lengend Snippet: The reduction of haematopoietic stem and progenitor cells (HSPCs) caused by the induced deletion of miR17~92 can be fully prevented by the loss of pro-apoptotic BIM. a–c C57BL/6 wt mice (Ly5.1+) were lethally irradiated and reconstituted with a 1:1 mixture of bone marrow cells from UBC-GFP mice (competitor cells) and bone marrow cells from mice of the indicated genotypes (test cells). Reconstituted mice were treated with tamoxifen 8–15 weeks post-transplantation to activate the CreERT2 recombinase. After an additional 8–10 weeks the haematopoietic stem/progenitor cell populations (HSPC) indicated were identified by staining with antibodies against cell type specific surface markers. Representative FACS plots for the gating strategies are provided for wt mice in Fig. ​Fig.3.3. Percentages of GFP-negative cells (test cells) were determined for each cell subset. Data represent mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (Student's t test miR17~92fl/fl; RosaCreERT2+/Ki vs. wt, RosaCreERT2+/Ki, miR17~92fl/fl; RosaCreERT2+/Ki; Bim−/−, Bim−/−, miR17~92fl/fl; RosaCreERT2+/Ki; Puma−/− and Puma−/− mice). n = number of donor/number of recipient mice as indicated

Article Snippet: HSPCs were analysed by staining with fluorochrome-conjugated monoclonal antibodies against the following cell surface markers: CD150-BV421 (clone TC15-12F12.2, Biolegend), CD127-APC (IL-7 receptor, clone A7R34, eBioscience), CD48-PECy7 (clone HM48-1, eBioscience), CD105-PE (clone MJ718, eBioscience), CD34-A647 (clone RAM34, BD), CD117-BV711 (clone 2B8, BD), CD16/32-PerCPCy5.5 (clone 2.4G2, BD), CD135-PE (eBioscience), CD41-PECy7 (clone MWReg30, BD), SCA-1-A595, CD127-APCCy7 (eBioscience), CD9-A647, CD16_32-FITC, CD41-PECy7, CD2-A700, CD4-A700, CD8-A700, GR-1-A700, F4/80-A700, CD19-A700, B220-A700, Ly6G-A700, TER-119-A700 and NK1.1-A700 [ 27 ].

Techniques: Irradiation, Transplantation Assay, Staining

The induced deletion of miR17~92 causes a substantial reduction in lineage-committed haematopoietic progenitor cells and this can be completely prevented by the concomitant loss of BIM. C57BL/6 wt mice (Ly5.1+) were lethally irradiated and reconstituted with a 1:1 mixture of bone marrow cells from UBC-GFP mice (competitor cells) and bone marrow cells from mice of the indicated genotypes (test cells). Reconstituted mice were treated with tamoxifen 8–10 weeks post-transplantation to activate the CreERT2 recombinase. After an additional 8–10 weeks, the haematopoietic progenitor cell populations were identified by staining with antibodies against cell type specific surface markers as indicated in the representative FACS plots. The percentages of GFP-negative cells (test cells) were determined for the indicated cell populations. Data represent mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (Student's t test miR17~92fl/fl; RosaCreERT2+/Ki vs. wt, RosaCreERT2+/Ki, miR17~92fl/fl; RosaCreERT2+/Ki; Bim−/− and Bim−/− mice). Representative FACS plots indicating the gating strategy and staining for the cell surface markers used are provided for wt mice. a Lineage-committed progenitor cells, including CLP (common lymphoid progenitor), CMP (common myeloid progenitor), GMP (granulocyte/macrophage progenitor) and MEP (megakaryocyte/erythroid progenitor) populations, b granulocyte progenitors, including the pre-GM and GM cell populations, and c pre-megakaryocyte/erythroid progenitors (Pre-MegE), megakaryocyte (MK progenitors) and the erythroid progenitors, pre-CFU-E (colony forming unit erythroid) and CFU-E were examined in the competitive haematopoietic reconstitution assay using staining for the indicated markers

Journal: Cell Death and Differentiation

Article Title: miR17~92 restrains pro-apoptotic BIM to ensure survival of haematopoietic stem and progenitor cells

doi: 10.1038/s41418-019-0430-6

Figure Lengend Snippet: The induced deletion of miR17~92 causes a substantial reduction in lineage-committed haematopoietic progenitor cells and this can be completely prevented by the concomitant loss of BIM. C57BL/6 wt mice (Ly5.1+) were lethally irradiated and reconstituted with a 1:1 mixture of bone marrow cells from UBC-GFP mice (competitor cells) and bone marrow cells from mice of the indicated genotypes (test cells). Reconstituted mice were treated with tamoxifen 8–10 weeks post-transplantation to activate the CreERT2 recombinase. After an additional 8–10 weeks, the haematopoietic progenitor cell populations were identified by staining with antibodies against cell type specific surface markers as indicated in the representative FACS plots. The percentages of GFP-negative cells (test cells) were determined for the indicated cell populations. Data represent mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (Student's t test miR17~92fl/fl; RosaCreERT2+/Ki vs. wt, RosaCreERT2+/Ki, miR17~92fl/fl; RosaCreERT2+/Ki; Bim−/− and Bim−/− mice). Representative FACS plots indicating the gating strategy and staining for the cell surface markers used are provided for wt mice. a Lineage-committed progenitor cells, including CLP (common lymphoid progenitor), CMP (common myeloid progenitor), GMP (granulocyte/macrophage progenitor) and MEP (megakaryocyte/erythroid progenitor) populations, b granulocyte progenitors, including the pre-GM and GM cell populations, and c pre-megakaryocyte/erythroid progenitors (Pre-MegE), megakaryocyte (MK progenitors) and the erythroid progenitors, pre-CFU-E (colony forming unit erythroid) and CFU-E were examined in the competitive haematopoietic reconstitution assay using staining for the indicated markers

Article Snippet: HSPCs were analysed by staining with fluorochrome-conjugated monoclonal antibodies against the following cell surface markers: CD150-BV421 (clone TC15-12F12.2, Biolegend), CD127-APC (IL-7 receptor, clone A7R34, eBioscience), CD48-PECy7 (clone HM48-1, eBioscience), CD105-PE (clone MJ718, eBioscience), CD34-A647 (clone RAM34, BD), CD117-BV711 (clone 2B8, BD), CD16/32-PerCPCy5.5 (clone 2.4G2, BD), CD135-PE (eBioscience), CD41-PECy7 (clone MWReg30, BD), SCA-1-A595, CD127-APCCy7 (eBioscience), CD9-A647, CD16_32-FITC, CD41-PECy7, CD2-A700, CD4-A700, CD8-A700, GR-1-A700, F4/80-A700, CD19-A700, B220-A700, Ly6G-A700, TER-119-A700 and NK1.1-A700 [ 27 ].

Techniques: Irradiation, Transplantation Assay, Staining, Reconstitution Assay

miR17~92 is critical for the survival of human CD34+ haematopoietic stem/progenitor cells (HSPCs) by restraining BIM-induced apoptosis. a Human CD34+ cord blood cells where cultured with 20 µM MIR17PTi [29] or scrambled control (scr) and the total numbers of colonies were counted and calculated as relative to no drug treated samples. Data are presented as mean ± SEM from two human CD34+ cord blood cell samples, each tested in duplicates. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. b human CD34+ cord blood cells (n = 5) were transfected in triplicates with the indicated mirScript target protectors (Qiagen) together with a reporter plasmid encoding for GFP. At 48 h after transfection the cell viability of the transfected (GFP+) cells was assessed by flow cytometric analysis upon staining with AnnexinV-647 and DAPI with viable cells defined as AnnexinV negative/DAPI negative. Data (normalised to scrambled (scr) control samples) are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (Student's t test comparing individual target protectors to the scr control)

Journal: Cell Death and Differentiation

Article Title: miR17~92 restrains pro-apoptotic BIM to ensure survival of haematopoietic stem and progenitor cells

doi: 10.1038/s41418-019-0430-6

Figure Lengend Snippet: miR17~92 is critical for the survival of human CD34+ haematopoietic stem/progenitor cells (HSPCs) by restraining BIM-induced apoptosis. a Human CD34+ cord blood cells where cultured with 20 µM MIR17PTi [29] or scrambled control (scr) and the total numbers of colonies were counted and calculated as relative to no drug treated samples. Data are presented as mean ± SEM from two human CD34+ cord blood cell samples, each tested in duplicates. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. b human CD34+ cord blood cells (n = 5) were transfected in triplicates with the indicated mirScript target protectors (Qiagen) together with a reporter plasmid encoding for GFP. At 48 h after transfection the cell viability of the transfected (GFP+) cells was assessed by flow cytometric analysis upon staining with AnnexinV-647 and DAPI with viable cells defined as AnnexinV negative/DAPI negative. Data (normalised to scrambled (scr) control samples) are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (Student's t test comparing individual target protectors to the scr control)

Article Snippet: HSPCs were analysed by staining with fluorochrome-conjugated monoclonal antibodies against the following cell surface markers: CD150-BV421 (clone TC15-12F12.2, Biolegend), CD127-APC (IL-7 receptor, clone A7R34, eBioscience), CD48-PECy7 (clone HM48-1, eBioscience), CD105-PE (clone MJ718, eBioscience), CD34-A647 (clone RAM34, BD), CD117-BV711 (clone 2B8, BD), CD16/32-PerCPCy5.5 (clone 2.4G2, BD), CD135-PE (eBioscience), CD41-PECy7 (clone MWReg30, BD), SCA-1-A595, CD127-APCCy7 (eBioscience), CD9-A647, CD16_32-FITC, CD41-PECy7, CD2-A700, CD4-A700, CD8-A700, GR-1-A700, F4/80-A700, CD19-A700, B220-A700, Ly6G-A700, TER-119-A700 and NK1.1-A700 [ 27 ].

Techniques: Cell Culture, Transfection, Plasmid Preparation, Staining

Induced deletion of the miR17~92 cluster causes a loss of haematopoietic stem and progenitor cells (HSPCs). C57BL/6 wt mice (Ly5.1+) were lethally irradiated and reconstituted with a 1:1 mixture of E14.5 foetal liver cells from UBC-GFP embryos (competitor cells) and E14.5 foetal liver cells from miR17~92deldel, miR17~92+/del or wt embryos (test cells). At 8–10 weeks after transplantation, the fraction of reconstituted test cells was determined by FACS analysis gating on Ly5.1+GFP− cells (donor test cells). Representative FACS plots for the gating strategies are provided for wt mice. Percentages of GFP-negative cells (test cells) were determined for the indicated cell populations. Data represent mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (Student's t test miR17~92deldel, miR17~92+/del and wt). a LSK (lineage−c-KIT+SCA-1+) cells and progenitor cells (lineage−c-KIT+SCA-1−) were identified in the lineage marker negative compartment (TER119−, CD4−, CD8a−, Ly6G−, B220−, CD2−, CD3−, CD19−, F4/80−, NK1.1−, GR-1−) by staining with antibodies against c-KIT and SCA-1. b Oligopotent progenitors, including common myeloid progenitors (CMP), granulocyte/macrophage progenitors (GMP) and megakaryocyte/erythroid progenitors, were identified by cell surface staining for the SLAM markers (CD16/32, CD150, CD34) in the progenitor cell population (lineage−c-KIT+SCA-1−). Common lymphoid progenitors (CLP) were identified as c-KITmedSCA-1medIL-7 receptor+ within the lineage marker negative cell populations. c Long-term haematopoietic stem cells (LT-HSC), short-term haematopoietic stem cells (ST-HSC) as well as multipotent progenitor cells (MPP) were identified using the FLK series markers (CD34, CD135) within the LSK cell population. d Haematopoietic stem cells (HSC), multipotent progenitor cells (MPP) and haematopoietic progenitor cells (HPC-1/2) were identified using cell surface staining for the SLAM markers (CD48, CD150) within the LSK cell population. n = number of donor/number of recipient mice as indicated

Journal: Cell Death and Differentiation

Article Title: miR17~92 restrains pro-apoptotic BIM to ensure survival of haematopoietic stem and progenitor cells

doi: 10.1038/s41418-019-0430-6

Figure Lengend Snippet: Induced deletion of the miR17~92 cluster causes a loss of haematopoietic stem and progenitor cells (HSPCs). C57BL/6 wt mice (Ly5.1+) were lethally irradiated and reconstituted with a 1:1 mixture of E14.5 foetal liver cells from UBC-GFP embryos (competitor cells) and E14.5 foetal liver cells from miR17~92deldel, miR17~92+/del or wt embryos (test cells). At 8–10 weeks after transplantation, the fraction of reconstituted test cells was determined by FACS analysis gating on Ly5.1+GFP− cells (donor test cells). Representative FACS plots for the gating strategies are provided for wt mice. Percentages of GFP-negative cells (test cells) were determined for the indicated cell populations. Data represent mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (Student's t test miR17~92deldel, miR17~92+/del and wt). a LSK (lineage−c-KIT+SCA-1+) cells and progenitor cells (lineage−c-KIT+SCA-1−) were identified in the lineage marker negative compartment (TER119−, CD4−, CD8a−, Ly6G−, B220−, CD2−, CD3−, CD19−, F4/80−, NK1.1−, GR-1−) by staining with antibodies against c-KIT and SCA-1. b Oligopotent progenitors, including common myeloid progenitors (CMP), granulocyte/macrophage progenitors (GMP) and megakaryocyte/erythroid progenitors, were identified by cell surface staining for the SLAM markers (CD16/32, CD150, CD34) in the progenitor cell population (lineage−c-KIT+SCA-1−). Common lymphoid progenitors (CLP) were identified as c-KITmedSCA-1medIL-7 receptor+ within the lineage marker negative cell populations. c Long-term haematopoietic stem cells (LT-HSC), short-term haematopoietic stem cells (ST-HSC) as well as multipotent progenitor cells (MPP) were identified using the FLK series markers (CD34, CD135) within the LSK cell population. d Haematopoietic stem cells (HSC), multipotent progenitor cells (MPP) and haematopoietic progenitor cells (HPC-1/2) were identified using cell surface staining for the SLAM markers (CD48, CD150) within the LSK cell population. n = number of donor/number of recipient mice as indicated

Article Snippet: HSPCs were analysed by staining with fluorochrome-conjugated monoclonal antibodies against the following cell surface markers: CD150-BV421 (clone TC15-12F12.2, Biolegend), CD127-APC (IL-7 receptor, clone A7R34, eBioscience), CD48-PECy7 (clone HM48-1, eBioscience), CD105-PE (clone MJ718, eBioscience), CD34-A647 (clone RAM34, BD), CD117-BV711 (clone 2B8, BD), CD16/32-PerCPCy5.5 (clone 2.4G2, BD), CD135-PE (eBioscience), CD41-PECy7 (clone MWReg30, BD), SCA-1-A595, CD127-APCCy7 (eBioscience), CD9-A647, CD16_32-FITC, CD41-PECy7, CD2-A700, CD4-A700, CD8-A700, GR-1-A700, F4/80-A700, CD19-A700, B220-A700, Ly6G-A700, TER-119-A700 and NK1.1-A700 [ 27 ].

Techniques: Irradiation, Transplantation Assay, Marker, Staining