Journal: Stem Cell Research & Therapy

Article Title: SIRT1-modified human umbilical cord mesenchymal stem cells ameliorate experimental peritoneal fibrosis by inhibiting the TGF-β/Smad3 pathway

doi: 10.1186/s13287-020-01878-2

Figure Lengend Snippet: Oligonucleotide primer sequences for qRT-PCR

Article Snippet: The primary antibody of SIRT1 (ab110304, 1:1000 dilution), TGF-β (ab92486, 1:1000 dilution), α-SMA (ab265588, 1:1500 dilution), Fibronectin (ab268021, 1:2000 dilution), IL-6 (ab233706, 1:1000 dilution), IL-1β (ab234437, 1:1500 dilution), and Smad3 (ab40854, 1:2000 dilution) were purchased from Abcam (Cambridge, UK); pSmad3 (#8769, 1:1500 dilution), TNF-α (#3707, 1:1500 dilution), MCP-1 (#12199, 1:1000 dilution), Calretinin (#17114, 1:2000 dilution), Snail (#3879, 1:1000 dilution), and β-Actin (#4970, 1:3000 dilution) were obtained from Cell Signaling Technology, Inc. (Danvers, USA).

Techniques: Sequencing

Journal: Stem Cell Research & Therapy

Article Title: SIRT1-modified human umbilical cord mesenchymal stem cells ameliorate experimental peritoneal fibrosis by inhibiting the TGF-β/Smad3 pathway

doi: 10.1186/s13287-020-01878-2

Figure Lengend Snippet: SIRT1-modified hUCMSCs attenuated EMT in PD-induced peritoneal damage. a Immunohistochemical analyses of α-SMA expression in peritoneal tissues (bar 200 μm). b Accumulation of α-SMA-positive area of a . qRT-PCR was used to measure the mRNA levels of α-SMA ( c ), Fibronectin ( d ), Col III ( e ), Snail ( f ), and Calretinin ( g ) in the peritoneal omentum tissues from the indicated groups. h Western blot was used to measure the protein levels of Fibronectin, α-SMA, Snail, and Calretinin in the indicated conditions, and the relative expressions were normalized to control ( i ). Data are presented as mean ± SD. n = 8 for each group. * p < 0.05, ** p < 0.01, and *** p < 0.001 between the indicated groups

Article Snippet: The primary antibody of SIRT1 (ab110304, 1:1000 dilution), TGF-β (ab92486, 1:1000 dilution), α-SMA (ab265588, 1:1500 dilution), Fibronectin (ab268021, 1:2000 dilution), IL-6 (ab233706, 1:1000 dilution), IL-1β (ab234437, 1:1500 dilution), and Smad3 (ab40854, 1:2000 dilution) were purchased from Abcam (Cambridge, UK); pSmad3 (#8769, 1:1500 dilution), TNF-α (#3707, 1:1500 dilution), MCP-1 (#12199, 1:1000 dilution), Calretinin (#17114, 1:2000 dilution), Snail (#3879, 1:1000 dilution), and β-Actin (#4970, 1:3000 dilution) were obtained from Cell Signaling Technology, Inc. (Danvers, USA).

Techniques: Modification, Immunohistochemical staining, Expressing, Quantitative RT-PCR, Western Blot, Control