Journal: Acta neuropathologica communications

Article Title: BDNF augmentation reverses cranial radiation therapy-induced cognitive decline and neurodegenerative consequences.

doi: 10.1186/s40478-024-01906-9

Figure Lengend Snippet: Fig. 2 Riluzole treatment restores hippocampal BDNF in the irradiated hippocampus. 10–12 weeks old WT male mice received cranial radiation therapy (RT) followed by riluzole (RZ) treatment (13 mg/kg) in drinking water for 6–7 weeks. An ELISA-based quantification of BDNF from the micro-dissected hippocampus showed RT-induced reductions in the RT + Vehicle group. Importantly, RZ treatment in the cranially irradiated mice showed significant restoration of BDNF levels. Data are presented as mean ± SEM (N = 6–10 mice per group). P values were derived from two-way ANOVA and Bonferroni’s multiple comparisons test

Article Snippet: BDNF levels were quantified using a commercially available ELISA kit (E-EL-M0203, Elabscience Biotechnology) and uncoated ELISA plates (Nunc MaxiSorp, Biolegend).

Techniques: Irradiation, Enzyme-linked Immunosorbent Assay, Derivative Assay

Journal: bioRxiv

Article Title: Identification of a neural basis for anorexia nervosa

doi: 10.64898/2026.02.07.704578

Figure Lengend Snippet: (A) Representative images showing GFP and c-Fos (arrows) expression patterns in the PVT, a downstream target of the VMH, in AAV-DIO-EYFP, AAV-DIO-NaChBac+sgRNA, AAV-DIO-NaChBac+sgVglut2 injection SF1-Cre::ERa-Cre mice. scale bar=250 um. (B) Representative images showing GFP (green) and c-Fos (red, arrows) expression patterns in the PVT, a downstream target of the VMH, in SF1-Cre::ERa-Cre::BDNFflox/++EYFP, SF1-Cre::ERa-Cre::BDNFflox/++NaChBac, SF1-Cre::ERa-Cre::BDNFflox/flox+NaChBac mice. scale bar=250 um. (C) Number of c-Fos positive cells in the PVT region related to panel A. n=4-5 per group; one-way ANOVA; ***P<0.001 versus EYFP; ###P<0.001 versus NaChBac+sgRNA. (D) number of c-Fos positive cells in the PVT region related to panel A. n=4-5 per group; one-way ANOVA; ***P<0.001 versus EYFP; ###P<0.001 versus SF1-Cre::ERa-Cre:: BDNFflox/flox+NaChBac. (E) Diagram of viral delivery (AAV-DIO-mCherry, AAV-DIO-BDNF-mCherry+sgRNA, AAV-DIO-BDNF-mCherry+sgVglut2) into the VMH of SF1-Cre::ERa-Cre mice. (F) Body weight trajectories of SF1-Cre::ERa-Cre mice after VH injection of virus. n=4 (EYFP, BDNF+sgVglut2), n=5 (BDNF+sgRNA); two-way ANOVA; *P<0.05, **P<0.01, ***P<0.001 versus mCherry; #P<0.05 versus BDNF+sgRNA. (G) Daily food intake. n=4-5 per group, one-way ANOVA, **P=0.006 versus mCherry; #P=0.043 versus BDNF+sgRNA. (H) Total number of jumps during a 10-min test. n=4-5 per group; one-way ANOVA; ***P<0.01 versus mCherry; ###P<0.001 versus BDNF+sgRNA. (I, J) time spent in the light room in light-dark test (I), social time with stranger in three-chamber test (J). n=4-5 per group; one-way ANOVA; *P<0.05, ***P<0.01 versus mCherry; #P<0.05 versus BDNF+sgRNA. (K) Representative images showing mCherry (green) and c-Fos (magenta, arrows) expression patterns in the PVT, a downstream of VMH, in mCherry, BDNF+sgRNA, BDNF+sgVglut2 injection SF1-Cre::ERa-Cre mice. scale bar=250 um. (L) number of c-Fos positive cells in PVT region related to panel K. n=4-5 per group; one-way ANOVA; ***P<0.001 versus mCherry; ###P<0.001 versus BDNF+sgRNA.

Article Snippet: The following primary antibodies were used: rabbit anti-cFos (1:1000, #2250, Cell Signaling Technology), rabbit anti-BDNF(1:200, ANT-010, Alomone Labs), goat anti-GFP (1:1000, 51370, ROCKLAND), rabbit anti-ER alpha (1:500, NBP1-84827), chicken anti-mCherry (1:500, MCHERRY-0020, aves labs).The next day, sections were rinsed three times in PBS with 0.1% Triton X-100 and incubated with secondary antibodies prepared in the same diluent (secondary antibody, 5% normal donkey serum, 0.1% Triton X-100 in PBS).

Techniques: Expressing, Injection, Virus

Journal: bioRxiv

Article Title: Identification of a neural basis for anorexia nervosa

doi: 10.64898/2026.02.07.704578

Figure Lengend Snippet: Representative images showing mCherry (green) and c-Fos (magenta, arrows) expression pattern in lateral septum (A), preoptic area (C), PAG (E), periLC (G). scale bar=250 μm. The pictures in the bottom row of each panel represent the magnified pictures shown in the boxed area in each column. Number of cFos positive cells in in lateral septum (B), preoptic area (D), PAG (F, periLC (H). n=4-5 per group; one-way ANOVA; ***P<0.001 versus mCherry; ###P<0.001 versus BDNF+sgRNA.

Article Snippet: The following primary antibodies were used: rabbit anti-cFos (1:1000, #2250, Cell Signaling Technology), rabbit anti-BDNF(1:200, ANT-010, Alomone Labs), goat anti-GFP (1:1000, 51370, ROCKLAND), rabbit anti-ER alpha (1:500, NBP1-84827), chicken anti-mCherry (1:500, MCHERRY-0020, aves labs).The next day, sections were rinsed three times in PBS with 0.1% Triton X-100 and incubated with secondary antibodies prepared in the same diluent (secondary antibody, 5% normal donkey serum, 0.1% Triton X-100 in PBS).

Techniques: Expressing

Journal: bioRxiv

Article Title: Identification of a neural basis for anorexia nervosa

doi: 10.64898/2026.02.07.704578

Figure Lengend Snippet: Representative images showing mCherry (red) and cFos (cyan, arrows) expression pattern in VH in mCherry, BDNF+sgRNA, BDNF+sgVglut2 injection SF1-Cre::ERa-Cre mice (related to ). Scale bar=200 μm.

Article Snippet: The following primary antibodies were used: rabbit anti-cFos (1:1000, #2250, Cell Signaling Technology), rabbit anti-BDNF(1:200, ANT-010, Alomone Labs), goat anti-GFP (1:1000, 51370, ROCKLAND), rabbit anti-ER alpha (1:500, NBP1-84827), chicken anti-mCherry (1:500, MCHERRY-0020, aves labs).The next day, sections were rinsed three times in PBS with 0.1% Triton X-100 and incubated with secondary antibodies prepared in the same diluent (secondary antibody, 5% normal donkey serum, 0.1% Triton X-100 in PBS).

Techniques: Expressing, Injection

Journal: The European journal of neuroscience

Article Title: Cholesterol-recognition motifs in the transmembrane domain of the tyrosine kinase receptor family: The case of TRKB.

doi: 10.1111/ejn.15218

Figure Lengend Snippet: FIGURE 1 (a) Workflow of data mining. The TMR (transmembrane sequence +5 amino acid residues from each N- and C-terminal sides) of curated entries found in UniProt using the code for tyrosine-kinase receptor family (2.7.10.1) were screened for the presence of cholesterol recognition and alignment consensus (CRAC and CARC). The incidence of CRAC and CARC motifs in (b) human, (c) mouse, (d) zebrafish, (e) fruit fly, and (f) C. elegans TMR of RTK family members. (g) Library of CRAC and CARC sequences (all combinations used can be found in the stored data). Residue sequence predicted to be embedded into the cell membrane shown in yellow

Article Snippet: The plates were washed 3× with PBS buffer, and a mixture of biotinylated BDNF (bBDNF: 0, 0.05, 0.1, 0.5, 1, 5, or 10 ng/ml, Alomone Labs, #B- 250- B) and cholesterol (0, 20, 50 or 100 μM) was added for 1h at room temperature, followed by washing with PBS.

Techniques: Sequencing, Residue, Membrane

Journal: The European journal of neuroscience

Article Title: Cholesterol-recognition motifs in the transmembrane domain of the tyrosine kinase receptor family: The case of TRKB.

doi: 10.1111/ejn.15218

Figure Lengend Snippet: FIGURE 3 TRKB interaction with cholesterol. (a,b) Snapshots of the interaction between TRKB and cholesterol at the TMR (see supplement video) indicating that cholesterol (orange) interacts with the OH- group (red) in Y433 (cyan) of the TRKB.TMR (green). The TRKB.TMR helix is shown in the cartoon, the lipids and Y433 sidechain in van der Waals representations. (c) Molecular dynamics simulations show that cholesterol predominantly resides close to the C-alpha atom of Y433 but not C-alpha atom of R427, highlighting the significance of the aromatic Y in the TRKB CARC domain. (d) The binding of biotinylated BDNF (bBDNF) to TRKB is modulated by added cholesterol in a bell-shaped fashion [from Scatchard analysis Kd for bBDNF ctrl: 2.77 pM; chol 20 μM: 2.27pM; chol 50 μM: 2.61 pM; chol 100 μM: 1.93 pM; Bmax for bBDNF ctrl: 96.18; chol 20 μM: 108.9; chol 50 μM: 80.81; chol 100 μM: 65.35]. Curves from the Scatchard transformation of the TRKB:BDNF binding are depicted in the insert. Data expressed as mean/SEM of binding normalized by bBDNF10 ng/ml ctrl (no added cholesterol). (e) Expansion of the binding of bBDNF 1 ng/ml (black circles) and bBDNF 0 (ctrl, open circles), depicting the bell-shaped effect of different concentrations of cholesterol on bBDNF:TRKB interaction. Data expressed as mean/SEM of binding normalized by bBDNF 1 ng/ml ctrl. (f) Unlabeled BDNF (0-20 ng/ ml) dislocates bBDNF (1 ng/ml) binding to immobilized TRKB assayed at no supplemented cholesterol (0 μM). Data expressed as mean/SEM normalized by bBDNF 1 ng/ml at BDNF 0 ng/ml. *p < .05 from control (BDNF 1 ng/ml–cholesterol ctrl, R427)

Article Snippet: The plates were washed 3× with PBS buffer, and a mixture of biotinylated BDNF (bBDNF: 0, 0.05, 0.1, 0.5, 1, 5, or 10 ng/ml, Alomone Labs, #B- 250- B) and cholesterol (0, 20, 50 or 100 μM) was added for 1h at room temperature, followed by washing with PBS.

Techniques: Binding Assay, Transformation Assay, Control

Journal: Brain Sciences

Article Title: Neonatal Handling Positively Modulates Anxiety, Sensorimotor Gating, Working Memory, and Cortico-Hippocampal Neuroplastic Adaptations in Two Genetically Selected Rat Strains Differing in Emotional and Cognitive Traits

doi: 10.3390/brainsci15080776

Figure Lengend Snippet: Western blot analysis of the BDNF ( A , B ), trkB ( C , D ), and the PSA-NCAM ( E , F ), in the prefrontal (prelimbic/infralimbic) cortex of the RHA and the RLA rats, either untreated controls (CTRL) or treated with neonatal handling (NH). The values represent the densitometric analysis of the BDNF/GAPDH ( B ), trkB/GAPDH ( D ), and the PSA-NCAM/GAPDH ( F ) band grey optical density (O.D.) ratios. The bars denote the mean ± S.E.M. of 9–10 rats, in each experimental group. ***: p < 0.001 (post hoc Duncan’s multiple range test).

Article Snippet: Molecular weight (mw) standards (Precision Plus Protein Western C Standards, Cat# 161–0376, Bio-Rad, Hercules, CA, USA) and recombinant human BDNF protein (rhBDNF) (Cat# B-257, Alomone Labs, Jerusalem, Israel) were run in parallel.

Techniques: Western Blot

Journal: Brain Sciences

Article Title: Neonatal Handling Positively Modulates Anxiety, Sensorimotor Gating, Working Memory, and Cortico-Hippocampal Neuroplastic Adaptations in Two Genetically Selected Rat Strains Differing in Emotional and Cognitive Traits

doi: 10.3390/brainsci15080776

Figure Lengend Snippet: Western blot analysis of the BDNF ( A , B ), trkB ( C , D ), and the PSA-NCAM ( E , F ), in the anterior cingulate cortex of the RHA and the RLA rats, either untreated controls (CTRL) or treated with neonatal handling (NH). The values represent the densitometric analysis of the BDNF/GAPDH ( B ), trkB/GAPDH ( D ), and the PSA-NCAM/GAPDH ( F ) band grey optical density (O.D.) ratios. The bars denote the mean ± S.E.M. of 9–10 rats, in each experimental group. **: p < 0.01 (post hoc Duncan’s multiple range test).

Article Snippet: Molecular weight (mw) standards (Precision Plus Protein Western C Standards, Cat# 161–0376, Bio-Rad, Hercules, CA, USA) and recombinant human BDNF protein (rhBDNF) (Cat# B-257, Alomone Labs, Jerusalem, Israel) were run in parallel.

Techniques: Western Blot

Journal: Brain Sciences

Article Title: Neonatal Handling Positively Modulates Anxiety, Sensorimotor Gating, Working Memory, and Cortico-Hippocampal Neuroplastic Adaptations in Two Genetically Selected Rat Strains Differing in Emotional and Cognitive Traits

doi: 10.3390/brainsci15080776

Figure Lengend Snippet: Western blot analysis of the BDNF ( A , B ), trkB ( C , D ), and the PSA-NCAM ( E , F ), in the ventral hippocampus of the RHA and the RLA rats, either untreated controls (CTRL) or treated with neonatal handling (NH). The values represent the densitometric analysis of the BDNF/GAPDH ( B ), trkB/GAPDH ( D ), and the PSA-NCAM/GAPDH ( F ) band grey optical density (O.D.) ratios. The bars denote the mean ± S.E.M. of 9–10 rats, in each experimental group. *: p < 0.05; **: p < 0.02 (post hoc Duncan’s multiple range test).

Article Snippet: Molecular weight (mw) standards (Precision Plus Protein Western C Standards, Cat# 161–0376, Bio-Rad, Hercules, CA, USA) and recombinant human BDNF protein (rhBDNF) (Cat# B-257, Alomone Labs, Jerusalem, Israel) were run in parallel.

Techniques: Western Blot

Journal: Brain Sciences

Article Title: Neonatal Handling Positively Modulates Anxiety, Sensorimotor Gating, Working Memory, and Cortico-Hippocampal Neuroplastic Adaptations in Two Genetically Selected Rat Strains Differing in Emotional and Cognitive Traits

doi: 10.3390/brainsci15080776

Figure Lengend Snippet: Western blot analysis of the BDNF ( A , B ), trkB ( C , D ), and the PSA-NCAM ( E , F ), in the dorsal hippocampus of the RHA and the RLA rats, either untreated controls (CTRL) or treated with neonatal handling (NH). The values represent the densitometric analysis of the BDNF/GAPDH ( B ), trkB/GAPDH ( D ), and the PSA-NCAM/GAPDH ( F ) band grey optical density (O.D.) ratios. The bars denote the mean ± S.E.M. of 9–10 rats, in each experimental group.

Article Snippet: Molecular weight (mw) standards (Precision Plus Protein Western C Standards, Cat# 161–0376, Bio-Rad, Hercules, CA, USA) and recombinant human BDNF protein (rhBDNF) (Cat# B-257, Alomone Labs, Jerusalem, Israel) were run in parallel.

Techniques: Western Blot

Journal: FEBS letters

Article Title: miR-190a inhibits epithelial-mesenchymal transition of hepatoma cells via targeting the long non-coding RNA treRNA.

doi: 10.1016/j.febslet.2015.11.024

Figure Lengend Snippet: Fig. 3. Suppression of treRNA by miR-190a inhibits EMT in HCC. (A) 20 nM miR-190a, 20 nM siRNA duplexes against treRNA (si-treRNA1 or sitreRNA2), 20 nM miR-190a, or NC RNA was transfected into Huh-7 or HepG2 cells. EMT related genes were quantified 72 h post-transfection as indicated. (B) Cells were treated with antagomiR (anti-miR- 190a) or NC RNA as in (A). The mRNA level of different EMT genes was quantified and normalized to b-actin. (C) Cell lysates were blotting with EMT marker E-cadherin (E-cad), Claudin-1 (Claud), Vimentin (Vimen), N-cadherin (N-cad), SNAI1 (Snail). b-Actin was used as endogenous control. The EMT marker expression level in was calculated using Image J and presented as histogram (right panel). (D) Cells were treated with TGF-b for 12 h and transfected with 20 nM miR-190a, 20 nM siRNA duplexes against treRNA (si-treRNA1 or si-treRNA2) or NC RNA subsequently. The morphological change was captured 24 h after transfection.

Article Snippet: Membranes were blocked by 5% BSA or 5% non-fat milk for 3 h at 25 C and incubated with primary antibody against Ago2 (#2897, Cell Signaling, US), Dicer (#5362, Cell Signaling), E-Cadherin (#3195, Cell Signaling), Claudin-1 (#13255, Cell Signaling), Vimentin (#5741, Cell Signaling), N-Cadherin (#13116, Cell Signaling), SNAI1 (#3897, Cell Signaling), or b-actin (C-4, Santa cruz, US) for 12 h at 4 C. Membranes were incubated with secondary antibody diluted in Tris-buffered saline supplemented with 0.5% Tween-20 and 5% BSA or 5% non-fat milk for 1 h at 25 C. The signal was detected with the enhanced chemiluminescence system (Pierce, US) and exposed to X-ray film (Kodak, US).

Techniques: Transfection, Marker, Control, Expressing