Journal: Pharmaceuticals

Article Title: Thymoquinone Enhances Apoptosis of K562 Chronic Myeloid Leukemia Cells through Hypomethylation of SHP-1 and Inhibition of JAK/STAT Signaling Pathway

doi: 10.3390/ph16060884

Figure Lengend Snippet: The percentage of CpG sites methylated at the SHP-1 promoter.

Article Snippet: Completely methylated and unmethylated human genomic DNA (Bisulfite-treated) and completely unmethylated DNA (Bisulfite-untreated) were purchased from Qiagen (Qiagen, Hilden, Germany) and used as controls.

Techniques: Methylation, Standard Deviation

Journal: Frontiers in Immunology

Article Title: A Systematic Review of Tissue and Single Cell Transcriptome/Proteome Studies of the Brain in Multiple Sclerosis

doi: 10.3389/fimmu.2022.761225

Figure Lengend Snippet: An overview of the advantages and disadvantages of the omics techniques.

Article Snippet: Epigenomics , Molecular changes on the DNA , Assessment of variability of factors that regulate the genome without changing the DNA sequence , WGBS (whole genome bisulfite-treated DNA sequencing) , Methylome-wide pattern and alterations , Moderate , Complex data analysis, lack of functional knowledge on methylation at other sites , Whole methylome state on single base pair level.

Techniques: Sequencing, Genome Wide, DNA Sequencing, Functional Assay, Methylation, Microarray, Hybridization, Immunoprecipitation, DNA Binding Assay, Binding Assay, Gene Expression, Amplification, Expressing, Clone Assay, Multiplexing, Protein-Protein interactions, Blocking Assay, Microscopy, Activity Assay, Mass Spectrometry, Mass Cytometry

Journal: Oncology Letters

Article Title: Related to testes-specific, vespid and pathogenesis protein-1 is regulated by methylation in glioblastoma

doi: 10.3892/ol.2014.1829

Figure Lengend Snippet: (A) Specific CpG-site RTVP-1 methylation in GBM (black), oligodendroglioma (grey) and NTB (white). P<0.001 for CpG 3, CpG 4, CpG 5 and CpG 6 (GBM vs. others). Bars represent median and interquartile range; whiskers represent range.. (B) RTVP-1 expression levels (quantitative polymerase chain reaction) in GBM compared with NTB sample (P<0.001). (C) Correlation between RTVP-1 methylation and expression. GBM, glioblastoma; NTB, non-tumor brain tissue; RTVP-1, related to testes-specific, vespid and pathogenesis protein-1.

Article Snippet: The method used for methylation analysis in the present study (Sequenom mass spectrometry detection of T-cleaved products of bisulfite-treated DNA) provided quantitative data through the ability to assess single-CpG methylation.

Techniques: Methylation, Expressing, Real-time Polymerase Chain Reaction

Journal: Oncology Letters

Article Title: Related to testes-specific, vespid and pathogenesis protein-1 is regulated by methylation in glioblastoma

doi: 10.3892/ol.2014.1829

Figure Lengend Snippet: Kaplan-Meier plot to determine overall survival of glioblastoma patients based on RTVP-1 promoter methylation status: RTVP-1 high-meth is indicated by the black line and RTVP-1 low-meth by the grey dotted line. No statistically significant difference was identified. RTVP-1, related to testes-specific, vespid and pathogenesis protein-1.

Article Snippet: The method used for methylation analysis in the present study (Sequenom mass spectrometry detection of T-cleaved products of bisulfite-treated DNA) provided quantitative data through the ability to assess single-CpG methylation.

Techniques: Methylation