Journal: Chemical biology & drug design

Article Title: Whole-cell biocatalysis platform for gram-scale oxidative dearomatization of phenols

doi: 10.1111/cbdd.13443

Figure Lengend Snippet: Examples of WC biocatalytic processes. (a) Select scaffolds accessed through WC biocatalytic reactions.[18–24] (b) WC biocatalysis utilizing a multi-enzyme cascade to generate chiral benzylic amines.[17]

Article Snippet: [ 20 – 22 ] Recently, researchers at GlaxoSmithKline demonstrated that a WC biocatalysis platform is also useful for discovery of new synthetic routes on the gram-scale, developing several biocatalytic routes to chiral 1,3-substituted cyclohexanones, such as 2 .

Techniques:

Journal: Chemical biology & drug design

Article Title: Whole-cell biocatalysis platform for gram-scale oxidative dearomatization of phenols

doi: 10.1111/cbdd.13443

Figure Lengend Snippet: Workflow of whole-cell reaction versus purified enzyme for TropB biocatalysis. Plasmid containing tropB is transformed into E. coli and then expressed. E. coli cells harboring TropB are harvested by centrifugation and frozen. The pellet can be added directly to the reaction flask. Additional steps are required to prepare purified protein.

Article Snippet: [ 20 – 22 ] Recently, researchers at GlaxoSmithKline demonstrated that a WC biocatalysis platform is also useful for discovery of new synthetic routes on the gram-scale, developing several biocatalytic routes to chiral 1,3-substituted cyclohexanones, such as 2 .

Techniques: Purification, Plasmid Preparation, Transformation Assay, Centrifugation