anti-gata4 Search Results


92
Developmental Studies Hybridoma Bank anti gata4
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Thermo Fisher gene exp gata4 hs00171403 m1
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Boster Bio rabbit anti gata 4
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Agenus Inc gata-4 antibody-specific staining
Effects of modified Davidsonʼs fluid (mDF) and 4% paraformaldehyde (PFA) on antigen preservation in Xiang pig testes. L and H represent low and high magnification, respectively. Testes were removed from 120-day Xiang pigs (n=6) and fixed with mDF and 4% PFA at 4°C for 24 h. A1–A4, C1–C4, and E1–E4 represent images of mDF-fixed testes, while B1–B4, D1–D4, and F1–F4 represent images of 4% PFA-fixed testes. Testicular cross-sections were immunostained with primary antibodies for DDx4 (A3–A4 and B3–B4), 3β-HSD (C3–C4 and D3–D4), <t>and</t> <t>GATA-binding</t> factor 4 (GATA4) (E3–E4 and F3–F4). Negative controls used phosphate buffered saline (PBS) for incubation (A1–A2, B1–B2, C1–C2, D1–D2, E1–E2, and F1–F2). Bars = 200 μm (A1–F1) and (A3–F3) and 10 μm (A2–F2) and (A4–F4). Positive immunostaining is shown in brown color after counterstaining with hematoxylin. DDx4 and 3β-HSD expressions were evident in germ and Leydig cells of 4% PFA-fixed testes but not in mDF-fixed testes. GATA4 was clearly expressed in 4% PFA-fixed <t>Sertoli</t> cells and weakly in Leydig cells (F4, Rectangle mark), but mDF-fixed testes showed inconspicuous immunostaining in these cells.
Gata 4 Antibody Specific Staining, supplied by Agenus Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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GeneTex anti-gata4 gtx113194
Effects of modified Davidsonʼs fluid (mDF) and 4% paraformaldehyde (PFA) on antigen preservation in Xiang pig testes. L and H represent low and high magnification, respectively. Testes were removed from 120-day Xiang pigs (n=6) and fixed with mDF and 4% PFA at 4°C for 24 h. A1–A4, C1–C4, and E1–E4 represent images of mDF-fixed testes, while B1–B4, D1–D4, and F1–F4 represent images of 4% PFA-fixed testes. Testicular cross-sections were immunostained with primary antibodies for DDx4 (A3–A4 and B3–B4), 3β-HSD (C3–C4 and D3–D4), <t>and</t> <t>GATA-binding</t> factor 4 (GATA4) (E3–E4 and F3–F4). Negative controls used phosphate buffered saline (PBS) for incubation (A1–A2, B1–B2, C1–C2, D1–D2, E1–E2, and F1–F2). Bars = 200 μm (A1–F1) and (A3–F3) and 10 μm (A2–F2) and (A4–F4). Positive immunostaining is shown in brown color after counterstaining with hematoxylin. DDx4 and 3β-HSD expressions were evident in germ and Leydig cells of 4% PFA-fixed testes but not in mDF-fixed testes. GATA4 was clearly expressed in 4% PFA-fixed <t>Sertoli</t> cells and weakly in Leydig cells (F4, Rectangle mark), but mDF-fixed testes showed inconspicuous immunostaining in these cells.
Anti Gata4 Gtx113194, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Merck KGaA gata binding factor-4 gata4
Effects of modified Davidsonʼs fluid (mDF) and 4% paraformaldehyde (PFA) on antigen preservation in Xiang pig testes. L and H represent low and high magnification, respectively. Testes were removed from 120-day Xiang pigs (n=6) and fixed with mDF and 4% PFA at 4°C for 24 h. A1–A4, C1–C4, and E1–E4 represent images of mDF-fixed testes, while B1–B4, D1–D4, and F1–F4 represent images of 4% PFA-fixed testes. Testicular cross-sections were immunostained with primary antibodies for DDx4 (A3–A4 and B3–B4), 3β-HSD (C3–C4 and D3–D4), <t>and</t> <t>GATA-binding</t> factor 4 (GATA4) (E3–E4 and F3–F4). Negative controls used phosphate buffered saline (PBS) for incubation (A1–A2, B1–B2, C1–C2, D1–D2, E1–E2, and F1–F2). Bars = 200 μm (A1–F1) and (A3–F3) and 10 μm (A2–F2) and (A4–F4). Positive immunostaining is shown in brown color after counterstaining with hematoxylin. DDx4 and 3β-HSD expressions were evident in germ and Leydig cells of 4% PFA-fixed testes but not in mDF-fixed testes. GATA4 was clearly expressed in 4% PFA-fixed <t>Sertoli</t> cells and weakly in Leydig cells (F4, Rectangle mark), but mDF-fixed testes showed inconspicuous immunostaining in these cells.
Gata Binding Factor 4 Gata4, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Affinity Biosciences anti-gata4
Effects of modified Davidsonʼs fluid (mDF) and 4% paraformaldehyde (PFA) on antigen preservation in Xiang pig testes. L and H represent low and high magnification, respectively. Testes were removed from 120-day Xiang pigs (n=6) and fixed with mDF and 4% PFA at 4°C for 24 h. A1–A4, C1–C4, and E1–E4 represent images of mDF-fixed testes, while B1–B4, D1–D4, and F1–F4 represent images of 4% PFA-fixed testes. Testicular cross-sections were immunostained with primary antibodies for DDx4 (A3–A4 and B3–B4), 3β-HSD (C3–C4 and D3–D4), <t>and</t> <t>GATA-binding</t> factor 4 (GATA4) (E3–E4 and F3–F4). Negative controls used phosphate buffered saline (PBS) for incubation (A1–A2, B1–B2, C1–C2, D1–D2, E1–E2, and F1–F2). Bars = 200 μm (A1–F1) and (A3–F3) and 10 μm (A2–F2) and (A4–F4). Positive immunostaining is shown in brown color after counterstaining with hematoxylin. DDx4 and 3β-HSD expressions were evident in germ and Leydig cells of 4% PFA-fixed testes but not in mDF-fixed testes. GATA4 was clearly expressed in 4% PFA-fixed <t>Sertoli</t> cells and weakly in Leydig cells (F4, Rectangle mark), but mDF-fixed testes showed inconspicuous immunostaining in these cells.
Anti Gata4, supplied by Affinity Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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94
Bioss gata4 polyclonal antibody
Effects of modified Davidsonʼs fluid (mDF) and 4% paraformaldehyde (PFA) on antigen preservation in Xiang pig testes. L and H represent low and high magnification, respectively. Testes were removed from 120-day Xiang pigs (n=6) and fixed with mDF and 4% PFA at 4°C for 24 h. A1–A4, C1–C4, and E1–E4 represent images of mDF-fixed testes, while B1–B4, D1–D4, and F1–F4 represent images of 4% PFA-fixed testes. Testicular cross-sections were immunostained with primary antibodies for DDx4 (A3–A4 and B3–B4), 3β-HSD (C3–C4 and D3–D4), <t>and</t> <t>GATA-binding</t> factor 4 (GATA4) (E3–E4 and F3–F4). Negative controls used phosphate buffered saline (PBS) for incubation (A1–A2, B1–B2, C1–C2, D1–D2, E1–E2, and F1–F2). Bars = 200 μm (A1–F1) and (A3–F3) and 10 μm (A2–F2) and (A4–F4). Positive immunostaining is shown in brown color after counterstaining with hematoxylin. DDx4 and 3β-HSD expressions were evident in germ and Leydig cells of 4% PFA-fixed testes but not in mDF-fixed testes. GATA4 was clearly expressed in 4% PFA-fixed <t>Sertoli</t> cells and weakly in Leydig cells (F4, Rectangle mark), but mDF-fixed testes showed inconspicuous immunostaining in these cells.
Gata4 Polyclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Boster Bio anti-gata4 (q258) antibody
Effects of modified Davidsonʼs fluid (mDF) and 4% paraformaldehyde (PFA) on antigen preservation in Xiang pig testes. L and H represent low and high magnification, respectively. Testes were removed from 120-day Xiang pigs (n=6) and fixed with mDF and 4% PFA at 4°C for 24 h. A1–A4, C1–C4, and E1–E4 represent images of mDF-fixed testes, while B1–B4, D1–D4, and F1–F4 represent images of 4% PFA-fixed testes. Testicular cross-sections were immunostained with primary antibodies for DDx4 (A3–A4 and B3–B4), 3β-HSD (C3–C4 and D3–D4), <t>and</t> <t>GATA-binding</t> factor 4 (GATA4) (E3–E4 and F3–F4). Negative controls used phosphate buffered saline (PBS) for incubation (A1–A2, B1–B2, C1–C2, D1–D2, E1–E2, and F1–F2). Bars = 200 μm (A1–F1) and (A3–F3) and 10 μm (A2–F2) and (A4–F4). Positive immunostaining is shown in brown color after counterstaining with hematoxylin. DDx4 and 3β-HSD expressions were evident in germ and Leydig cells of 4% PFA-fixed testes but not in mDF-fixed testes. GATA4 was clearly expressed in 4% PFA-fixed <t>Sertoli</t> cells and weakly in Leydig cells (F4, Rectangle mark), but mDF-fixed testes showed inconspicuous immunostaining in these cells.
Anti Gata4 (Q258) Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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93
Bio-Techne corporation human gata-4 biotinylated antibody
Effects of modified Davidsonʼs fluid (mDF) and 4% paraformaldehyde (PFA) on antigen preservation in Xiang pig testes. L and H represent low and high magnification, respectively. Testes were removed from 120-day Xiang pigs (n=6) and fixed with mDF and 4% PFA at 4°C for 24 h. A1–A4, C1–C4, and E1–E4 represent images of mDF-fixed testes, while B1–B4, D1–D4, and F1–F4 represent images of 4% PFA-fixed testes. Testicular cross-sections were immunostained with primary antibodies for DDx4 (A3–A4 and B3–B4), 3β-HSD (C3–C4 and D3–D4), <t>and</t> <t>GATA-binding</t> factor 4 (GATA4) (E3–E4 and F3–F4). Negative controls used phosphate buffered saline (PBS) for incubation (A1–A2, B1–B2, C1–C2, D1–D2, E1–E2, and F1–F2). Bars = 200 μm (A1–F1) and (A3–F3) and 10 μm (A2–F2) and (A4–F4). Positive immunostaining is shown in brown color after counterstaining with hematoxylin. DDx4 and 3β-HSD expressions were evident in germ and Leydig cells of 4% PFA-fixed testes but not in mDF-fixed testes. GATA4 was clearly expressed in 4% PFA-fixed <t>Sertoli</t> cells and weakly in Leydig cells (F4, Rectangle mark), but mDF-fixed testes showed inconspicuous immunostaining in these cells.
Human Gata 4 Biotinylated Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Effects of modified Davidsonʼs fluid (mDF) and 4% paraformaldehyde (PFA) on antigen preservation in Xiang pig testes. L and H represent low and high magnification, respectively. Testes were removed from 120-day Xiang pigs (n=6) and fixed with mDF and 4% PFA at 4°C for 24 h. A1–A4, C1–C4, and E1–E4 represent images of mDF-fixed testes, while B1–B4, D1–D4, and F1–F4 represent images of 4% PFA-fixed testes. Testicular cross-sections were immunostained with primary antibodies for DDx4 (A3–A4 and B3–B4), 3β-HSD (C3–C4 and D3–D4), and GATA-binding factor 4 (GATA4) (E3–E4 and F3–F4). Negative controls used phosphate buffered saline (PBS) for incubation (A1–A2, B1–B2, C1–C2, D1–D2, E1–E2, and F1–F2). Bars = 200 μm (A1–F1) and (A3–F3) and 10 μm (A2–F2) and (A4–F4). Positive immunostaining is shown in brown color after counterstaining with hematoxylin. DDx4 and 3β-HSD expressions were evident in germ and Leydig cells of 4% PFA-fixed testes but not in mDF-fixed testes. GATA4 was clearly expressed in 4% PFA-fixed Sertoli cells and weakly in Leydig cells (F4, Rectangle mark), but mDF-fixed testes showed inconspicuous immunostaining in these cells.

Journal: Journal of Toxicologic Pathology

Article Title: Effects of 4% paraformaldehyde and modified Davidson’s fluid on the morphology and immunohistochemistry of Xiang pig testes

doi: 10.1293/tox.2019-0072

Figure Lengend Snippet: Effects of modified Davidsonʼs fluid (mDF) and 4% paraformaldehyde (PFA) on antigen preservation in Xiang pig testes. L and H represent low and high magnification, respectively. Testes were removed from 120-day Xiang pigs (n=6) and fixed with mDF and 4% PFA at 4°C for 24 h. A1–A4, C1–C4, and E1–E4 represent images of mDF-fixed testes, while B1–B4, D1–D4, and F1–F4 represent images of 4% PFA-fixed testes. Testicular cross-sections were immunostained with primary antibodies for DDx4 (A3–A4 and B3–B4), 3β-HSD (C3–C4 and D3–D4), and GATA-binding factor 4 (GATA4) (E3–E4 and F3–F4). Negative controls used phosphate buffered saline (PBS) for incubation (A1–A2, B1–B2, C1–C2, D1–D2, E1–E2, and F1–F2). Bars = 200 μm (A1–F1) and (A3–F3) and 10 μm (A2–F2) and (A4–F4). Positive immunostaining is shown in brown color after counterstaining with hematoxylin. DDx4 and 3β-HSD expressions were evident in germ and Leydig cells of 4% PFA-fixed testes but not in mDF-fixed testes. GATA4 was clearly expressed in 4% PFA-fixed Sertoli cells and weakly in Leydig cells (F4, Rectangle mark), but mDF-fixed testes showed inconspicuous immunostaining in these cells.

Article Snippet: Accordingly, GATA-4 antibody-specific staining was apparent in 4% PFA-fixed Sertoli cells and its IOD was about 76.4% higher than that of mDF-fixed Sertoli cells ( Fig. 4.

Techniques: Modification, Preserving, Binding Assay, Incubation, Immunostaining