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Image Search Results
Journal: Journal of Toxicologic Pathology
Article Title: Effects of 4% paraformaldehyde and modified Davidson’s fluid on the morphology and immunohistochemistry of Xiang pig testes
doi: 10.1293/tox.2019-0072
Figure Lengend Snippet: Effects of modified Davidsonʼs fluid (mDF) and 4% paraformaldehyde (PFA) on antigen preservation in Xiang pig testes. L and H represent low and high magnification, respectively. Testes were removed from 120-day Xiang pigs (n=6) and fixed with mDF and 4% PFA at 4°C for 24 h. A1–A4, C1–C4, and E1–E4 represent images of mDF-fixed testes, while B1–B4, D1–D4, and F1–F4 represent images of 4% PFA-fixed testes. Testicular cross-sections were immunostained with primary antibodies for DDx4 (A3–A4 and B3–B4), 3β-HSD (C3–C4 and D3–D4), and GATA-binding factor 4 (GATA4) (E3–E4 and F3–F4). Negative controls used phosphate buffered saline (PBS) for incubation (A1–A2, B1–B2, C1–C2, D1–D2, E1–E2, and F1–F2). Bars = 200 μm (A1–F1) and (A3–F3) and 10 μm (A2–F2) and (A4–F4). Positive immunostaining is shown in brown color after counterstaining with hematoxylin. DDx4 and 3β-HSD expressions were evident in germ and Leydig cells of 4% PFA-fixed testes but not in mDF-fixed testes. GATA4 was clearly expressed in 4% PFA-fixed Sertoli cells and weakly in Leydig cells (F4, Rectangle mark), but mDF-fixed testes showed inconspicuous immunostaining in these cells.
Article Snippet: Accordingly,
Techniques: Modification, Preserving, Binding Assay, Incubation, Immunostaining