Journal: Nucleic Acids Research

Article Title: Lomofungin and dilomofungin: inhibitors of MBNL1-CUG RNA binding with distinct cellular effects

doi: 10.1093/nar/gku275

Figure Lengend Snippet: HTRF (black squares) and AlphaScreen (open circles) assays showed inhibition of biotin-CUG 12 binding to MBNL1-Δ105-His 6 protein by lomofungin. Subsequent analyses indicated two molecular species in the lomofungin stock. Error bars represent 1 s.d.

Article Snippet: The confirmatory assay employed a different detection system, consisting of AlphaScreen donor beads (conjugated to streptavidin) that emitted a singlet oxygen to activate AlphaScreen acceptor beads (coated with nickel, PerkinElmer).

Techniques: Amplified Luminescent Proximity Homogenous Assay, Inhibition, Binding Assay

Journal: Nucleic Acids Research

Article Title: Lomofungin and dilomofungin: inhibitors of MBNL1-CUG RNA binding with distinct cellular effects

doi: 10.1093/nar/gku275

Figure Lengend Snippet: AlphaScreen assay showing that dilomofungin (open triangles) is 17-fold more potent as inhibitor of MBNL1-(CUG) 12 binding than lomofungin (closed squares) [IC 50 s = 42 nM, 95% CI 33 to 53 nM, and 717 nM, 95% CI 533 to 964 nM, respectively].

Article Snippet: The confirmatory assay employed a different detection system, consisting of AlphaScreen donor beads (conjugated to streptavidin) that emitted a singlet oxygen to activate AlphaScreen acceptor beads (coated with nickel, PerkinElmer).

Techniques: Amplified Luminescent Proximity Homogenous Assay, Binding Assay

Journal: PLoS ONE

Article Title: Ramentaceone, a Naphthoquinone Derived from Drosera sp ., Induces Apoptosis by Suppressing PI3K/Akt Signaling in Breast Cancer Cells

doi: 10.1371/journal.pone.0147718

Figure Lengend Snippet: The AlphaScreen technology was used to determine enzyme activity. The AlphaScreen assay was performed with ramentaceone or LY294002, added at concentrations ranging from 100 μM to 0.01 nM, 25 ng PI3-kinase, 5 μM PIP 2 , 10 μM ATP and with an incubation time of 1 h. Values represent mean ± SD of three independent experiments.

Article Snippet: In order to detect and quantify PI3-kinase activity the Amplified Luminescent proximity Homogenous Assay (AlphaScreen) technology was employed using the PI3-kinase kit (K-1300), Echelon, USA).

Techniques: Amplified Luminescent Proximity Homogenous Assay, Activity Assay, Incubation