akt2 promoter Search Results


akt2 3 utr fragments  (Genecopoeia)
94
Genecopoeia akt2 3 utr fragments
miR-149-3p directly targets <t>AKT2</t> 3′-UTR. (A) Putative binding sites of miR-149-3p in the 3′-UTR of AKT2. (B and C) Relative luciferase activity of Cal27 and SCC-9 cells co-transfected with WT or MT AKT2 3′-UTR and miR-149-3p mimic or mimic control. (D and E) Reverse transcription-quantitative PCR and western blot analysis of AKT2 mRNA and protein expression levels in Cal27 and SCC-9 cells transfected with miR-149-3p mimic or mimic control. (F) miR-149-3p expression was correlated associated with AKT2 mRNA level in TCGA head and neck squamous cell carcinoma dataset. Data are presented as the mean ± SD. *P<0.05, **P<0.01 and ***P<0.001 vs. mimic control. miR-149-3p, microRNA-149-3p; WT, wild-type; MT, mutated; 3′-UTR, 3′-untranslated region.
Akt2 3 Utr Fragments, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/akt2+promoter/pmc07821286-68-6-17?v=Genecopoeia
Average 94 stars, based on 1 article reviews
akt2 3 utr fragments - by Bioz Stars, 2026-07
94/100 stars
  Buy from Supplier
promoter reporter clone for human akt2  (Genecopoeia)
94
Genecopoeia promoter reporter clone for human akt2
miR-149-3p directly targets <t>AKT2</t> 3′-UTR. (A) Putative binding sites of miR-149-3p in the 3′-UTR of AKT2. (B and C) Relative luciferase activity of Cal27 and SCC-9 cells co-transfected with WT or MT AKT2 3′-UTR and miR-149-3p mimic or mimic control. (D and E) Reverse transcription-quantitative PCR and western blot analysis of AKT2 mRNA and protein expression levels in Cal27 and SCC-9 cells transfected with miR-149-3p mimic or mimic control. (F) miR-149-3p expression was correlated associated with AKT2 mRNA level in TCGA head and neck squamous cell carcinoma dataset. Data are presented as the mean ± SD. *P<0.05, **P<0.01 and ***P<0.001 vs. mimic control. miR-149-3p, microRNA-149-3p; WT, wild-type; MT, mutated; 3′-UTR, 3′-untranslated region.
Promoter Reporter Clone For Human Akt2, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/akt2+promoter/genecopoeia___promoter-reporter-clones-group?v=Genecopoeia
Average 94 stars, based on 1 article reviews
promoter reporter clone for human akt2 - by Bioz Stars, 2026-07
94/100 stars
  Buy from Supplier

Image Search Results


miR-149-3p directly targets AKT2 3′-UTR. (A) Putative binding sites of miR-149-3p in the 3′-UTR of AKT2. (B and C) Relative luciferase activity of Cal27 and SCC-9 cells co-transfected with WT or MT AKT2 3′-UTR and miR-149-3p mimic or mimic control. (D and E) Reverse transcription-quantitative PCR and western blot analysis of AKT2 mRNA and protein expression levels in Cal27 and SCC-9 cells transfected with miR-149-3p mimic or mimic control. (F) miR-149-3p expression was correlated associated with AKT2 mRNA level in TCGA head and neck squamous cell carcinoma dataset. Data are presented as the mean ± SD. *P<0.05, **P<0.01 and ***P<0.001 vs. mimic control. miR-149-3p, microRNA-149-3p; WT, wild-type; MT, mutated; 3′-UTR, 3′-untranslated region.

Journal: Molecular Medicine Reports

Article Title: MicroRNA-149-3p inhibits cell proliferation by targeting AKT2 in oral squamous cell carcinoma

doi: 10.3892/mmr.2020.11811

Figure Lengend Snippet: miR-149-3p directly targets AKT2 3′-UTR. (A) Putative binding sites of miR-149-3p in the 3′-UTR of AKT2. (B and C) Relative luciferase activity of Cal27 and SCC-9 cells co-transfected with WT or MT AKT2 3′-UTR and miR-149-3p mimic or mimic control. (D and E) Reverse transcription-quantitative PCR and western blot analysis of AKT2 mRNA and protein expression levels in Cal27 and SCC-9 cells transfected with miR-149-3p mimic or mimic control. (F) miR-149-3p expression was correlated associated with AKT2 mRNA level in TCGA head and neck squamous cell carcinoma dataset. Data are presented as the mean ± SD. *P<0.05, **P<0.01 and ***P<0.001 vs. mimic control. miR-149-3p, microRNA-149-3p; WT, wild-type; MT, mutated; 3′-UTR, 3′-untranslated region.

Article Snippet: The human wild-type (WT) and MT AKT2 3′-UTR fragments were inserted into the pEZX-FR02 luciferase reporter vector (GeneCopoeia, Inc.).

Techniques: Binding Assay, Luciferase, Activity Assay, Transfection, Control, Reverse Transcription, Real-time Polymerase Chain Reaction, Western Blot, Expressing

AKT2 restores the decreased proliferation induced by miR-149-3p mimic in oral squamous cell carcinoma cells. (A and B) Reverse transcription-quantitative PCR and western blot analyses of AKT2 expression levels in Cal27 and SCC-9 cells transfected with AKT2 plasmid or empty pcDNA3 plasmid. (C and D) Reverse transcription-quantitative PCR analysis of miR-149-3p expression levels in Cal27 and SCC-9 cells co-transfected with miR-149-3p mimic and AKT2 plasmid or empty pcDNA3 plasmid. (E and F) Reverse transcription-quantitative PCR and western blot analyses of AKT2 expression levels in Cal27 and SCC-9 cells co-transfected with miR-149-3p mimic and AKT2 plasmid or empty pcDNA3 plasmid. (G and H) Viability of Cal27 and SCC-9 cells co-transfected with miR-149-3p mimic and AKT2 plasmid or empty pcDNA3 plasmid, as determined using a Cell Counting Kit-8 assay. (I) Proliferation of Cal27 and SCC-9 cells co-transfected with miR-149-3p mimic and AKT2 plasmid or empty pcDNA3 plasmid was examined using a colony formation assay. Data are presented as the mean ± SD. **P<0.01 and ***P<0.001 vs. vector or as indicated. miR-149-3p, microRNA-149-3p; NS, non-significant.

Journal: Molecular Medicine Reports

Article Title: MicroRNA-149-3p inhibits cell proliferation by targeting AKT2 in oral squamous cell carcinoma

doi: 10.3892/mmr.2020.11811

Figure Lengend Snippet: AKT2 restores the decreased proliferation induced by miR-149-3p mimic in oral squamous cell carcinoma cells. (A and B) Reverse transcription-quantitative PCR and western blot analyses of AKT2 expression levels in Cal27 and SCC-9 cells transfected with AKT2 plasmid or empty pcDNA3 plasmid. (C and D) Reverse transcription-quantitative PCR analysis of miR-149-3p expression levels in Cal27 and SCC-9 cells co-transfected with miR-149-3p mimic and AKT2 plasmid or empty pcDNA3 plasmid. (E and F) Reverse transcription-quantitative PCR and western blot analyses of AKT2 expression levels in Cal27 and SCC-9 cells co-transfected with miR-149-3p mimic and AKT2 plasmid or empty pcDNA3 plasmid. (G and H) Viability of Cal27 and SCC-9 cells co-transfected with miR-149-3p mimic and AKT2 plasmid or empty pcDNA3 plasmid, as determined using a Cell Counting Kit-8 assay. (I) Proliferation of Cal27 and SCC-9 cells co-transfected with miR-149-3p mimic and AKT2 plasmid or empty pcDNA3 plasmid was examined using a colony formation assay. Data are presented as the mean ± SD. **P<0.01 and ***P<0.001 vs. vector or as indicated. miR-149-3p, microRNA-149-3p; NS, non-significant.

Article Snippet: The human wild-type (WT) and MT AKT2 3′-UTR fragments were inserted into the pEZX-FR02 luciferase reporter vector (GeneCopoeia, Inc.).

Techniques: Reverse Transcription, Real-time Polymerase Chain Reaction, Western Blot, Expressing, Transfection, Plasmid Preparation, Cell Counting, Colony Assay

AKT2 attenuates miR-149-3p-mediated sensitivity of oral squamous cell carcinoma cells to 5-Fu. (A and B) Cal27 and SCC-9 cells were co-transfected with miR-149-3p mimic and AKT2 plasmid or empty pcDNA3 plasmid, and were subsequently treated with 5-Fu for 24 h. Cell viability was determined using a Cell Counting Kit-8 assay and normalized to non-5-Fu treatment group. (C and D) Cal27 and SCC-9 cells were co-transfected with miR-149-3p mimic and AKT2 plasmid or empty pcDNA3 plasmid, and were treated with 5-Fu for 24 h. The protein expression levels of cleaved caspase-3 and cleaved PARP were detected by western blot analysis. Data are presented as the mean ± SD. ***P<0.001. miR-149-3p, microRNA-149-3p; 5-Fu, 5-fluorouracil.

Journal: Molecular Medicine Reports

Article Title: MicroRNA-149-3p inhibits cell proliferation by targeting AKT2 in oral squamous cell carcinoma

doi: 10.3892/mmr.2020.11811

Figure Lengend Snippet: AKT2 attenuates miR-149-3p-mediated sensitivity of oral squamous cell carcinoma cells to 5-Fu. (A and B) Cal27 and SCC-9 cells were co-transfected with miR-149-3p mimic and AKT2 plasmid or empty pcDNA3 plasmid, and were subsequently treated with 5-Fu for 24 h. Cell viability was determined using a Cell Counting Kit-8 assay and normalized to non-5-Fu treatment group. (C and D) Cal27 and SCC-9 cells were co-transfected with miR-149-3p mimic and AKT2 plasmid or empty pcDNA3 plasmid, and were treated with 5-Fu for 24 h. The protein expression levels of cleaved caspase-3 and cleaved PARP were detected by western blot analysis. Data are presented as the mean ± SD. ***P<0.001. miR-149-3p, microRNA-149-3p; 5-Fu, 5-fluorouracil.

Article Snippet: The human wild-type (WT) and MT AKT2 3′-UTR fragments were inserted into the pEZX-FR02 luciferase reporter vector (GeneCopoeia, Inc.).

Techniques: Transfection, Plasmid Preparation, Cell Counting, Expressing, Western Blot