RP0056B-100 Search Results


recombinant bovine il 17a  (Kingfisher Biotech)
94
Kingfisher Biotech recombinant bovine il 17a
Figure 5. Concentrations of chemoattractants and cytokines in milk samples of the 9 responsive cows. Concentrations were measured by ELISA in the milk samples of the 9 responder cows. Quarters were infused with 25 mg ovalbumin at time 0 and milk samples taken at indicated times. Median values (Q1, Q3) are shown. Concentrations varied significantly (Friedman test) as a function of hpi for C5a, CXCL8, IL-1b, IL-6, IFN-c, IL- <t>17A</t> (p,0.001) and CXCL3 (p = 0.002). doi:10.1371/journal.pone.0063471.g005
Recombinant Bovine Il 17a, supplied by Kingfisher Biotech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant bovine il 17a/product/Kingfisher Biotech
Average 94 stars, based on 1 article reviews
recombinant bovine il 17a - by Bioz Stars, 2026-04
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Figure 5. Concentrations of chemoattractants and cytokines in milk samples of the 9 responsive cows. Concentrations were measured by ELISA in the milk samples of the 9 responder cows. Quarters were infused with 25 mg ovalbumin at time 0 and milk samples taken at indicated times. Median values (Q1, Q3) are shown. Concentrations varied significantly (Friedman test) as a function of hpi for C5a, CXCL8, IL-1b, IL-6, IFN-c, IL- 17A (p,0.001) and CXCL3 (p = 0.002). doi:10.1371/journal.pone.0063471.g005

Journal: PloS one

Article Title: T helper 17-associated cytokines are produced during antigen-specific inflammation in the mammary gland.

doi: 10.1371/journal.pone.0063471

Figure Lengend Snippet: Figure 5. Concentrations of chemoattractants and cytokines in milk samples of the 9 responsive cows. Concentrations were measured by ELISA in the milk samples of the 9 responder cows. Quarters were infused with 25 mg ovalbumin at time 0 and milk samples taken at indicated times. Median values (Q1, Q3) are shown. Concentrations varied significantly (Friedman test) as a function of hpi for C5a, CXCL8, IL-1b, IL-6, IFN-c, IL- 17A (p,0.001) and CXCL3 (p = 0.002). doi:10.1371/journal.pone.0063471.g005

Article Snippet: Commercial affinity-purified antibodies to whole recombinant bovine IL-17A were from Kingfisher Biotech (St Paul, MN, USA), and rabbit antibody (ab79056) to human IL17A, raised against a synthetic 19 amino acid-peptide from near the center of human IL-17A and affinity-purified with the immunogen, was from Abcam.

Techniques: Enzyme-linked Immunosorbent Assay

Figure 9. Analysis by immunohistochemistry of representative tissue sections of uninfused, healthy mammary glands. A–B) Immunoreactivity of the apical side of the epithelial cells lining the alveoli to the N-term antibody to IL-17A of cows #1018 and 2014, respectively; C) Inhibition of labeling by Ab to N-terminal IL-17A peptide with the peptide antigen (cow #1018); D) Negative control with Ab to ovalbumin and second antibody conjugated to horseradish peroxidase (cow #1018); E–F) Immunoreactivity of the epithelial lining the alveoli to abcam antibody (cows #1018 and 2014, respectively); G–H) Immunoreactivity of mammary tissue of healthy uninfused quarters of cows 1018 and 2014 to the C-term antibody, respectively. Scale bars indicate 25 mm. doi:10.1371/journal.pone.0063471.g009

Journal: PloS one

Article Title: T helper 17-associated cytokines are produced during antigen-specific inflammation in the mammary gland.

doi: 10.1371/journal.pone.0063471

Figure Lengend Snippet: Figure 9. Analysis by immunohistochemistry of representative tissue sections of uninfused, healthy mammary glands. A–B) Immunoreactivity of the apical side of the epithelial cells lining the alveoli to the N-term antibody to IL-17A of cows #1018 and 2014, respectively; C) Inhibition of labeling by Ab to N-terminal IL-17A peptide with the peptide antigen (cow #1018); D) Negative control with Ab to ovalbumin and second antibody conjugated to horseradish peroxidase (cow #1018); E–F) Immunoreactivity of the epithelial lining the alveoli to abcam antibody (cows #1018 and 2014, respectively); G–H) Immunoreactivity of mammary tissue of healthy uninfused quarters of cows 1018 and 2014 to the C-term antibody, respectively. Scale bars indicate 25 mm. doi:10.1371/journal.pone.0063471.g009

Article Snippet: Commercial affinity-purified antibodies to whole recombinant bovine IL-17A were from Kingfisher Biotech (St Paul, MN, USA), and rabbit antibody (ab79056) to human IL17A, raised against a synthetic 19 amino acid-peptide from near the center of human IL-17A and affinity-purified with the immunogen, was from Abcam.

Techniques: Immunohistochemistry, Inhibition, Labeling, Negative Control