Journal: Journal of Clinical Neurology (Seoul, Korea)

Article Title: Evaluating an In-House Cell-Based Assay for Detecting Antibodies Against Muscle-Specific Tyrosine Kinase in Myasthenia Gravis

doi: 10.3988/jcn.2021.17.3.400

Figure Lengend Snippet: Correlations between the tests to detect MuSK Abs. A: The correlation between the CBA and the previously performed RIPA was assessed in 20 samples from the patients with MG whose samples had previously been assessed using an RIPA, the results of which were reported as quantitative values. There was an almost perfect agreement between the results of the CBA measured by visual grading and the results of the previously conducted RIPA (Cohen's kappa=0.880, p <0.001). B: Correlation between the results obtained in the CBA and ELISA applied to the same samples was assessed in 177 samples from the patients with MG. There was also an almost perfect agreement between the results of the CBA and ELISA (Cohen's kappa=0.982, p <0.001). CBA: cell-based assay, ELISA: enzyme-linked immunosorbent assay, MG: myasthenia gravis, MuSK Abs: antibodies against muscle-specific tyrosine kinase, RIPA: radioimmunoprecipitation assay.

Article Snippet: All 218 serum samples were evaluated with MuSK Abs ELISA (IBL International, Männedorf, Germany) according to the manufacturer's instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Cell Based Assay, Radio Immunoprecipitation