Cell Fusion Search Results


99
ATCC atcc ccl 185ig cell line
Atcc Ccl 185ig Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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98
TaKaRa in fusion snap assembly system
In Fusion Snap Assembly System, supplied by TaKaRa, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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94
MedChemExpress ccnd1 sequences
Molecular mechanism of SHP2 inhibition in GIST cells. (A) Immunoblotting analysis of whole‐cell lysate from four GIST cell lines treated with SHP099 for 24 h. Statistical analysis of the ratios of p‐mTORC1/mTORC1 and p‐S6K/p70 S6K are presented ( n = 3). (B) Cell cycle and statistical analysis of the four GIST cell lines treated with SHP099 (10 and 20 µM) for 24 h ( n = 3). (C) Immunoblotting analysis of whole cell lysate from four GIST cells treated with SHP099 for 24 h. Statistical analysis of the ratio of p‐cyclin D1/cyclin D1, p‐Rb/Rb and p‐GSK3β/GSK3β are presented ( n = 3). (D) Immunoblotting analysis of whole‐cell lysates from four GIST cell lines under treatment of SHP099 (12 µM) and/or LiCl (20 mM) for 24 h. Statistical analysis is presented ( n = 3). (E) Cell cycle analysis and statistical analysis of the four GIST cell lines treated with SHP099 (12 µM) and/or LiCl (20 mM) for 24 h ( n = 3).
Ccnd1 Sequences, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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99
TaKaRa fusion ecodry master mix takarabio
Molecular mechanism of SHP2 inhibition in GIST cells. (A) Immunoblotting analysis of whole‐cell lysate from four GIST cell lines treated with SHP099 for 24 h. Statistical analysis of the ratios of p‐mTORC1/mTORC1 and p‐S6K/p70 S6K are presented ( n = 3). (B) Cell cycle and statistical analysis of the four GIST cell lines treated with SHP099 (10 and 20 µM) for 24 h ( n = 3). (C) Immunoblotting analysis of whole cell lysate from four GIST cells treated with SHP099 for 24 h. Statistical analysis of the ratio of p‐cyclin D1/cyclin D1, p‐Rb/Rb and p‐GSK3β/GSK3β are presented ( n = 3). (D) Immunoblotting analysis of whole‐cell lysates from four GIST cell lines under treatment of SHP099 (12 µM) and/or LiCl (20 mM) for 24 h. Statistical analysis is presented ( n = 3). (E) Cell cycle analysis and statistical analysis of the four GIST cell lines treated with SHP099 (12 µM) and/or LiCl (20 mM) for 24 h ( n = 3).
Fusion Ecodry Master Mix Takarabio, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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92
Sino Biological f protein
Molecular mechanism of SHP2 inhibition in GIST cells. (A) Immunoblotting analysis of whole‐cell lysate from four GIST cell lines treated with SHP099 for 24 h. Statistical analysis of the ratios of p‐mTORC1/mTORC1 and p‐S6K/p70 S6K are presented ( n = 3). (B) Cell cycle and statistical analysis of the four GIST cell lines treated with SHP099 (10 and 20 µM) for 24 h ( n = 3). (C) Immunoblotting analysis of whole cell lysate from four GIST cells treated with SHP099 for 24 h. Statistical analysis of the ratio of p‐cyclin D1/cyclin D1, p‐Rb/Rb and p‐GSK3β/GSK3β are presented ( n = 3). (D) Immunoblotting analysis of whole‐cell lysates from four GIST cell lines under treatment of SHP099 (12 µM) and/or LiCl (20 mM) for 24 h. Statistical analysis is presented ( n = 3). (E) Cell cycle analysis and statistical analysis of the four GIST cell lines treated with SHP099 (12 µM) and/or LiCl (20 mM) for 24 h ( n = 3).
F Protein, supplied by Sino Biological, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Becton Dickinson facs fusion cell sorters
Molecular mechanism of SHP2 inhibition in GIST cells. (A) Immunoblotting analysis of whole‐cell lysate from four GIST cell lines treated with SHP099 for 24 h. Statistical analysis of the ratios of p‐mTORC1/mTORC1 and p‐S6K/p70 S6K are presented ( n = 3). (B) Cell cycle and statistical analysis of the four GIST cell lines treated with SHP099 (10 and 20 µM) for 24 h ( n = 3). (C) Immunoblotting analysis of whole cell lysate from four GIST cells treated with SHP099 for 24 h. Statistical analysis of the ratio of p‐cyclin D1/cyclin D1, p‐Rb/Rb and p‐GSK3β/GSK3β are presented ( n = 3). (D) Immunoblotting analysis of whole‐cell lysates from four GIST cell lines under treatment of SHP099 (12 µM) and/or LiCl (20 mM) for 24 h. Statistical analysis is presented ( n = 3). (E) Cell cycle analysis and statistical analysis of the four GIST cell lines treated with SHP099 (12 µM) and/or LiCl (20 mM) for 24 h ( n = 3).
Facs Fusion Cell Sorters, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Feto Maternal and GenetYX Center trophoblast cell fusion
Molecular mechanism of SHP2 inhibition in GIST cells. (A) Immunoblotting analysis of whole‐cell lysate from four GIST cell lines treated with SHP099 for 24 h. Statistical analysis of the ratios of p‐mTORC1/mTORC1 and p‐S6K/p70 S6K are presented ( n = 3). (B) Cell cycle and statistical analysis of the four GIST cell lines treated with SHP099 (10 and 20 µM) for 24 h ( n = 3). (C) Immunoblotting analysis of whole cell lysate from four GIST cells treated with SHP099 for 24 h. Statistical analysis of the ratio of p‐cyclin D1/cyclin D1, p‐Rb/Rb and p‐GSK3β/GSK3β are presented ( n = 3). (D) Immunoblotting analysis of whole‐cell lysates from four GIST cell lines under treatment of SHP099 (12 µM) and/or LiCl (20 mM) for 24 h. Statistical analysis is presented ( n = 3). (E) Cell cycle analysis and statistical analysis of the four GIST cell lines treated with SHP099 (12 µM) and/or LiCl (20 mM) for 24 h ( n = 3).
Trophoblast Cell Fusion, supplied by Feto Maternal and GenetYX Center, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Becton Dickinson 400+fm/bd 400+mk
Molecular mechanism of SHP2 inhibition in GIST cells. (A) Immunoblotting analysis of whole‐cell lysate from four GIST cell lines treated with SHP099 for 24 h. Statistical analysis of the ratios of p‐mTORC1/mTORC1 and p‐S6K/p70 S6K are presented ( n = 3). (B) Cell cycle and statistical analysis of the four GIST cell lines treated with SHP099 (10 and 20 µM) for 24 h ( n = 3). (C) Immunoblotting analysis of whole cell lysate from four GIST cells treated with SHP099 for 24 h. Statistical analysis of the ratio of p‐cyclin D1/cyclin D1, p‐Rb/Rb and p‐GSK3β/GSK3β are presented ( n = 3). (D) Immunoblotting analysis of whole‐cell lysates from four GIST cell lines under treatment of SHP099 (12 µM) and/or LiCl (20 mM) for 24 h. Statistical analysis is presented ( n = 3). (E) Cell cycle analysis and statistical analysis of the four GIST cell lines treated with SHP099 (12 µM) and/or LiCl (20 mM) for 24 h ( n = 3).
400+Fm/Bd 400+Mk, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Becton Dickinson facsariatm fusion cell sorter
Molecular mechanism of SHP2 inhibition in GIST cells. (A) Immunoblotting analysis of whole‐cell lysate from four GIST cell lines treated with SHP099 for 24 h. Statistical analysis of the ratios of p‐mTORC1/mTORC1 and p‐S6K/p70 S6K are presented ( n = 3). (B) Cell cycle and statistical analysis of the four GIST cell lines treated with SHP099 (10 and 20 µM) for 24 h ( n = 3). (C) Immunoblotting analysis of whole cell lysate from four GIST cells treated with SHP099 for 24 h. Statistical analysis of the ratio of p‐cyclin D1/cyclin D1, p‐Rb/Rb and p‐GSK3β/GSK3β are presented ( n = 3). (D) Immunoblotting analysis of whole‐cell lysates from four GIST cell lines under treatment of SHP099 (12 µM) and/or LiCl (20 mM) for 24 h. Statistical analysis is presented ( n = 3). (E) Cell cycle analysis and statistical analysis of the four GIST cell lines treated with SHP099 (12 µM) and/or LiCl (20 mM) for 24 h ( n = 3).
Facsariatm Fusion Cell Sorter, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Becton Dickinson cell-sorting facs fusion
Molecular mechanism of SHP2 inhibition in GIST cells. (A) Immunoblotting analysis of whole‐cell lysate from four GIST cell lines treated with SHP099 for 24 h. Statistical analysis of the ratios of p‐mTORC1/mTORC1 and p‐S6K/p70 S6K are presented ( n = 3). (B) Cell cycle and statistical analysis of the four GIST cell lines treated with SHP099 (10 and 20 µM) for 24 h ( n = 3). (C) Immunoblotting analysis of whole cell lysate from four GIST cells treated with SHP099 for 24 h. Statistical analysis of the ratio of p‐cyclin D1/cyclin D1, p‐Rb/Rb and p‐GSK3β/GSK3β are presented ( n = 3). (D) Immunoblotting analysis of whole‐cell lysates from four GIST cell lines under treatment of SHP099 (12 µM) and/or LiCl (20 mM) for 24 h. Statistical analysis is presented ( n = 3). (E) Cell cycle analysis and statistical analysis of the four GIST cell lines treated with SHP099 (12 µM) and/or LiCl (20 mM) for 24 h ( n = 3).
Cell Sorting Facs Fusion, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Cosmo Bio USA genomonetm - cf ex sendai virus (hemagglutinating virus of japan or hvj) envelope cell fusion kit
Molecular mechanism of SHP2 inhibition in GIST cells. (A) Immunoblotting analysis of whole‐cell lysate from four GIST cell lines treated with SHP099 for 24 h. Statistical analysis of the ratios of p‐mTORC1/mTORC1 and p‐S6K/p70 S6K are presented ( n = 3). (B) Cell cycle and statistical analysis of the four GIST cell lines treated with SHP099 (10 and 20 µM) for 24 h ( n = 3). (C) Immunoblotting analysis of whole cell lysate from four GIST cells treated with SHP099 for 24 h. Statistical analysis of the ratio of p‐cyclin D1/cyclin D1, p‐Rb/Rb and p‐GSK3β/GSK3β are presented ( n = 3). (D) Immunoblotting analysis of whole‐cell lysates from four GIST cell lines under treatment of SHP099 (12 µM) and/or LiCl (20 mM) for 24 h. Statistical analysis is presented ( n = 3). (E) Cell cycle analysis and statistical analysis of the four GIST cell lines treated with SHP099 (12 µM) and/or LiCl (20 mM) for 24 h ( n = 3).
Genomonetm Cf Ex Sendai Virus (Hemagglutinating Virus Of Japan Or Hvj) Envelope Cell Fusion Kit, supplied by Cosmo Bio USA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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90
Janssen rsegfp2-tap1 fusion protein expressing u2os cell line
Molecular mechanism of SHP2 inhibition in GIST cells. (A) Immunoblotting analysis of whole‐cell lysate from four GIST cell lines treated with SHP099 for 24 h. Statistical analysis of the ratios of p‐mTORC1/mTORC1 and p‐S6K/p70 S6K are presented ( n = 3). (B) Cell cycle and statistical analysis of the four GIST cell lines treated with SHP099 (10 and 20 µM) for 24 h ( n = 3). (C) Immunoblotting analysis of whole cell lysate from four GIST cells treated with SHP099 for 24 h. Statistical analysis of the ratio of p‐cyclin D1/cyclin D1, p‐Rb/Rb and p‐GSK3β/GSK3β are presented ( n = 3). (D) Immunoblotting analysis of whole‐cell lysates from four GIST cell lines under treatment of SHP099 (12 µM) and/or LiCl (20 mM) for 24 h. Statistical analysis is presented ( n = 3). (E) Cell cycle analysis and statistical analysis of the four GIST cell lines treated with SHP099 (12 µM) and/or LiCl (20 mM) for 24 h ( n = 3).
Rsegfp2 Tap1 Fusion Protein Expressing U2os Cell Line, supplied by Janssen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Molecular mechanism of SHP2 inhibition in GIST cells. (A) Immunoblotting analysis of whole‐cell lysate from four GIST cell lines treated with SHP099 for 24 h. Statistical analysis of the ratios of p‐mTORC1/mTORC1 and p‐S6K/p70 S6K are presented ( n = 3). (B) Cell cycle and statistical analysis of the four GIST cell lines treated with SHP099 (10 and 20 µM) for 24 h ( n = 3). (C) Immunoblotting analysis of whole cell lysate from four GIST cells treated with SHP099 for 24 h. Statistical analysis of the ratio of p‐cyclin D1/cyclin D1, p‐Rb/Rb and p‐GSK3β/GSK3β are presented ( n = 3). (D) Immunoblotting analysis of whole‐cell lysates from four GIST cell lines under treatment of SHP099 (12 µM) and/or LiCl (20 mM) for 24 h. Statistical analysis is presented ( n = 3). (E) Cell cycle analysis and statistical analysis of the four GIST cell lines treated with SHP099 (12 µM) and/or LiCl (20 mM) for 24 h ( n = 3).

Journal: Clinical and Translational Medicine

Article Title: SHP2 inhibition and adjuvant therapy synergistically target KIT‐mutant GISTs via ERK1/2‐regulated GSK3β/cyclin D1 pathway

doi: 10.1002/ctm2.70231

Figure Lengend Snippet: Molecular mechanism of SHP2 inhibition in GIST cells. (A) Immunoblotting analysis of whole‐cell lysate from four GIST cell lines treated with SHP099 for 24 h. Statistical analysis of the ratios of p‐mTORC1/mTORC1 and p‐S6K/p70 S6K are presented ( n = 3). (B) Cell cycle and statistical analysis of the four GIST cell lines treated with SHP099 (10 and 20 µM) for 24 h ( n = 3). (C) Immunoblotting analysis of whole cell lysate from four GIST cells treated with SHP099 for 24 h. Statistical analysis of the ratio of p‐cyclin D1/cyclin D1, p‐Rb/Rb and p‐GSK3β/GSK3β are presented ( n = 3). (D) Immunoblotting analysis of whole‐cell lysates from four GIST cell lines under treatment of SHP099 (12 µM) and/or LiCl (20 mM) for 24 h. Statistical analysis is presented ( n = 3). (E) Cell cycle analysis and statistical analysis of the four GIST cell lines treated with SHP099 (12 µM) and/or LiCl (20 mM) for 24 h ( n = 3).

Article Snippet: Lentiviral plasmids harbouring PTPN11 or CCND1 sequences were constructed and subsequently co‐transfected into HEK 293T cells with helper psPAX2 and pMD2.G vectors using the polyethylenimine reagent (MCE).

Techniques: Inhibition, Western Blot, Cell Cycle Assay

Role of cyclin D1 in GIST cells. (A) Immunoblotting analysis of the indicated proteins in GIST cells following CCND1 knockout using the CRISPR/Cas9 system. Statistical analysis was presented for the ratio of cyclin D1 to β‐ACTIN and phosphorylated Rb to total Rb ( n = 3). (B) Analysis of cell proliferation in GIST cells following CCND1 gene knockout. Cell proliferation was assessed using the CCK‐8 assay ( n = 3). (C) Analysis of the effects of cyclin D1 overexpression in GIST cells. GIST cells were infected with recombinant lentivirus carrying CCND1 or control virus for 2 days. Then CCND1 ‐expressing GIST cells and control GIST cells (1 × 10 4 cells/well) were plated. These cells were co‐cultured with RMC4550 for 3 days. Absorbance was measured daily using the CCK‐8 assay ( n = 3).

Journal: Clinical and Translational Medicine

Article Title: SHP2 inhibition and adjuvant therapy synergistically target KIT‐mutant GISTs via ERK1/2‐regulated GSK3β/cyclin D1 pathway

doi: 10.1002/ctm2.70231

Figure Lengend Snippet: Role of cyclin D1 in GIST cells. (A) Immunoblotting analysis of the indicated proteins in GIST cells following CCND1 knockout using the CRISPR/Cas9 system. Statistical analysis was presented for the ratio of cyclin D1 to β‐ACTIN and phosphorylated Rb to total Rb ( n = 3). (B) Analysis of cell proliferation in GIST cells following CCND1 gene knockout. Cell proliferation was assessed using the CCK‐8 assay ( n = 3). (C) Analysis of the effects of cyclin D1 overexpression in GIST cells. GIST cells were infected with recombinant lentivirus carrying CCND1 or control virus for 2 days. Then CCND1 ‐expressing GIST cells and control GIST cells (1 × 10 4 cells/well) were plated. These cells were co‐cultured with RMC4550 for 3 days. Absorbance was measured daily using the CCK‐8 assay ( n = 3).

Article Snippet: Lentiviral plasmids harbouring PTPN11 or CCND1 sequences were constructed and subsequently co‐transfected into HEK 293T cells with helper psPAX2 and pMD2.G vectors using the polyethylenimine reagent (MCE).

Techniques: Western Blot, Knock-Out, CRISPR, Gene Knockout, CCK-8 Assay, Over Expression, Infection, Recombinant, Control, Virus, Expressing, Cell Culture