γh2ax Search Results


h2ax ps139 apc antibody  (Miltenyi Biotec)
95
Miltenyi Biotec h2ax ps139 apc antibody
H2ax Ps139 Apc Antibody, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti h2ax ps139 apc antibody  (Miltenyi Biotec)
95
Miltenyi Biotec anti h2ax ps139 apc antibody
Anti H2ax Ps139 Apc Antibody, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti h2ax ps139 apc antibody/product/Miltenyi Biotec
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γh2ax  (Miltenyi Biotec)
95
Miltenyi Biotec γh2ax
EA treatment alters mitochondrial dynamics, leading to the accumulation of intracellular ROS and DNA damage. Western blot analysis of the protein levels of Mfn2, Drp1, total-ERK, and p-ERK in the (A) A2780 monolayer, (B) A2780 spheroids, and (C) SKOV3 spheroids cells; A549-CD133 + (D,E) and SKOV3- spheroids (F,G) were treated with different concentrations of EA (0, 5, 10, 25 μM) for 24 h. Intracellular ROS accumulation was determined using flow cytometry. Representative histograms and corresponding bar diagrams showing ROS level in CSLCs; A549-CD133 + (H) and SKOV3- spheroid (I) cells were treated with different doses of EA for 48 h. <t>γH2AX</t> accumulation was detected through flow cytometry to evaluate the extent of DNA damage induced by EA. Representative contour plots obtained from flow cytometry analysis showing the percentage of γH2AX positive cells. Data was reanalyzed using FlowJo software. In all graphs, the results are represented as mean ± SD of triplicate experiments. ** P < 0.01, and *** P < 0.001.
γh2ax, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/γh2ax/product/Miltenyi Biotec
Average 95 stars, based on 1 article reviews
γh2ax - by Bioz Stars, 2026-04
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anti h2ax ps139 rea502  (Miltenyi Biotec)
95
Miltenyi Biotec anti h2ax ps139 rea502
EA treatment alters mitochondrial dynamics, leading to the accumulation of intracellular ROS and DNA damage. Western blot analysis of the protein levels of Mfn2, Drp1, total-ERK, and p-ERK in the (A) A2780 monolayer, (B) A2780 spheroids, and (C) SKOV3 spheroids cells; A549-CD133 + (D,E) and SKOV3- spheroids (F,G) were treated with different concentrations of EA (0, 5, 10, 25 μM) for 24 h. Intracellular ROS accumulation was determined using flow cytometry. Representative histograms and corresponding bar diagrams showing ROS level in CSLCs; A549-CD133 + (H) and SKOV3- spheroid (I) cells were treated with different doses of EA for 48 h. <t>γH2AX</t> accumulation was detected through flow cytometry to evaluate the extent of DNA damage induced by EA. Representative contour plots obtained from flow cytometry analysis showing the percentage of γH2AX positive cells. Data was reanalyzed using FlowJo software. In all graphs, the results are represented as mean ± SD of triplicate experiments. ** P < 0.01, and *** P < 0.001.
Anti H2ax Ps139 Rea502, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti h2ax ps139 rea502/product/Miltenyi Biotec
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anti-γh2ax  (Carl Zeiss)
90
Carl Zeiss anti-γh2ax
EA treatment alters mitochondrial dynamics, leading to the accumulation of intracellular ROS and DNA damage. Western blot analysis of the protein levels of Mfn2, Drp1, total-ERK, and p-ERK in the (A) A2780 monolayer, (B) A2780 spheroids, and (C) SKOV3 spheroids cells; A549-CD133 + (D,E) and SKOV3- spheroids (F,G) were treated with different concentrations of EA (0, 5, 10, 25 μM) for 24 h. Intracellular ROS accumulation was determined using flow cytometry. Representative histograms and corresponding bar diagrams showing ROS level in CSLCs; A549-CD133 + (H) and SKOV3- spheroid (I) cells were treated with different doses of EA for 48 h. <t>γH2AX</t> accumulation was detected through flow cytometry to evaluate the extent of DNA damage induced by EA. Representative contour plots obtained from flow cytometry analysis showing the percentage of γH2AX positive cells. Data was reanalyzed using FlowJo software. In all graphs, the results are represented as mean ± SD of triplicate experiments. ** P < 0.01, and *** P < 0.001.
Anti γh2ax, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-γh2ax/product/Carl Zeiss
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phospho-histone h2ax-s139 rabbit mab ap0687 antibody  (ABclonal Biotechnology)
90
ABclonal Biotechnology phospho-histone h2ax-s139 rabbit mab ap0687 antibody
EA treatment alters mitochondrial dynamics, leading to the accumulation of intracellular ROS and DNA damage. Western blot analysis of the protein levels of Mfn2, Drp1, total-ERK, and p-ERK in the (A) A2780 monolayer, (B) A2780 spheroids, and (C) SKOV3 spheroids cells; A549-CD133 + (D,E) and SKOV3- spheroids (F,G) were treated with different concentrations of EA (0, 5, 10, 25 μM) for 24 h. Intracellular ROS accumulation was determined using flow cytometry. Representative histograms and corresponding bar diagrams showing ROS level in CSLCs; A549-CD133 + (H) and SKOV3- spheroid (I) cells were treated with different doses of EA for 48 h. <t>γH2AX</t> accumulation was detected through flow cytometry to evaluate the extent of DNA damage induced by EA. Representative contour plots obtained from flow cytometry analysis showing the percentage of γH2AX positive cells. Data was reanalyzed using FlowJo software. In all graphs, the results are represented as mean ± SD of triplicate experiments. ** P < 0.01, and *** P < 0.001.
Phospho Histone H2ax S139 Rabbit Mab Ap0687 Antibody, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phospho-histone h2ax-s139 rabbit mab ap0687 antibody/product/ABclonal Biotechnology
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anti-phospho-histone h2a.x ser139 clone jbw301, 05-636  (Merck KGaA)
90
Merck KGaA anti-phospho-histone h2a.x ser139 clone jbw301, 05-636
EA treatment alters mitochondrial dynamics, leading to the accumulation of intracellular ROS and DNA damage. Western blot analysis of the protein levels of Mfn2, Drp1, total-ERK, and p-ERK in the (A) A2780 monolayer, (B) A2780 spheroids, and (C) SKOV3 spheroids cells; A549-CD133 + (D,E) and SKOV3- spheroids (F,G) were treated with different concentrations of EA (0, 5, 10, 25 μM) for 24 h. Intracellular ROS accumulation was determined using flow cytometry. Representative histograms and corresponding bar diagrams showing ROS level in CSLCs; A549-CD133 + (H) and SKOV3- spheroid (I) cells were treated with different doses of EA for 48 h. <t>γH2AX</t> accumulation was detected through flow cytometry to evaluate the extent of DNA damage induced by EA. Representative contour plots obtained from flow cytometry analysis showing the percentage of γH2AX positive cells. Data was reanalyzed using FlowJo software. In all graphs, the results are represented as mean ± SD of triplicate experiments. ** P < 0.01, and *** P < 0.001.
Anti Phospho Histone H2a.X Ser139 Clone Jbw301, 05 636, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
anti-phospho-histone h2a.x ser139 clone jbw301, 05-636 - by Bioz Stars, 2026-04
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mouse monoclonal antibodies to histone γ-h2ax  (Upstate Biotechnology Inc)
90
Upstate Biotechnology Inc mouse monoclonal antibodies to histone γ-h2ax
EA treatment alters mitochondrial dynamics, leading to the accumulation of intracellular ROS and DNA damage. Western blot analysis of the protein levels of Mfn2, Drp1, total-ERK, and p-ERK in the (A) A2780 monolayer, (B) A2780 spheroids, and (C) SKOV3 spheroids cells; A549-CD133 + (D,E) and SKOV3- spheroids (F,G) were treated with different concentrations of EA (0, 5, 10, 25 μM) for 24 h. Intracellular ROS accumulation was determined using flow cytometry. Representative histograms and corresponding bar diagrams showing ROS level in CSLCs; A549-CD133 + (H) and SKOV3- spheroid (I) cells were treated with different doses of EA for 48 h. <t>γH2AX</t> accumulation was detected through flow cytometry to evaluate the extent of DNA damage induced by EA. Representative contour plots obtained from flow cytometry analysis showing the percentage of γH2AX positive cells. Data was reanalyzed using FlowJo software. In all graphs, the results are represented as mean ± SD of triplicate experiments. ** P < 0.01, and *** P < 0.001.
Mouse Monoclonal Antibodies To Histone γ H2ax, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse monoclonal antibodies to histone γ-h2ax/product/Upstate Biotechnology Inc
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mouse anti–human cxcr3-percp-cy5.5 g025h7  (Becton Dickinson)
90
Becton Dickinson mouse anti–human cxcr3-percp-cy5.5 g025h7
EA treatment alters mitochondrial dynamics, leading to the accumulation of intracellular ROS and DNA damage. Western blot analysis of the protein levels of Mfn2, Drp1, total-ERK, and p-ERK in the (A) A2780 monolayer, (B) A2780 spheroids, and (C) SKOV3 spheroids cells; A549-CD133 + (D,E) and SKOV3- spheroids (F,G) were treated with different concentrations of EA (0, 5, 10, 25 μM) for 24 h. Intracellular ROS accumulation was determined using flow cytometry. Representative histograms and corresponding bar diagrams showing ROS level in CSLCs; A549-CD133 + (H) and SKOV3- spheroid (I) cells were treated with different doses of EA for 48 h. <t>γH2AX</t> accumulation was detected through flow cytometry to evaluate the extent of DNA damage induced by EA. Representative contour plots obtained from flow cytometry analysis showing the percentage of γH2AX positive cells. Data was reanalyzed using FlowJo software. In all graphs, the results are represented as mean ± SD of triplicate experiments. ** P < 0.01, and *** P < 0.001.
Mouse Anti–Human Cxcr3 Percp Cy5.5 G025h7, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti–human cxcr3-percp-cy5.5 g025h7/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
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γ-h2ax  (ImmunoWay Biotechnology Company)
90
ImmunoWay Biotechnology Company γ-h2ax
EA treatment alters mitochondrial dynamics, leading to the accumulation of intracellular ROS and DNA damage. Western blot analysis of the protein levels of Mfn2, Drp1, total-ERK, and p-ERK in the (A) A2780 monolayer, (B) A2780 spheroids, and (C) SKOV3 spheroids cells; A549-CD133 + (D,E) and SKOV3- spheroids (F,G) were treated with different concentrations of EA (0, 5, 10, 25 μM) for 24 h. Intracellular ROS accumulation was determined using flow cytometry. Representative histograms and corresponding bar diagrams showing ROS level in CSLCs; A549-CD133 + (H) and SKOV3- spheroid (I) cells were treated with different doses of EA for 48 h. <t>γH2AX</t> accumulation was detected through flow cytometry to evaluate the extent of DNA damage induced by EA. Representative contour plots obtained from flow cytometry analysis showing the percentage of γH2AX positive cells. Data was reanalyzed using FlowJo software. In all graphs, the results are represented as mean ± SD of triplicate experiments. ** P < 0.01, and *** P < 0.001.
γ H2ax, supplied by ImmunoWay Biotechnology Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/γ-h2ax/product/ImmunoWay Biotechnology Company
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h2ax antibody  (ZenBio)
90
ZenBio h2ax antibody
EA treatment alters mitochondrial dynamics, leading to the accumulation of intracellular ROS and DNA damage. Western blot analysis of the protein levels of Mfn2, Drp1, total-ERK, and p-ERK in the (A) A2780 monolayer, (B) A2780 spheroids, and (C) SKOV3 spheroids cells; A549-CD133 + (D,E) and SKOV3- spheroids (F,G) were treated with different concentrations of EA (0, 5, 10, 25 μM) for 24 h. Intracellular ROS accumulation was determined using flow cytometry. Representative histograms and corresponding bar diagrams showing ROS level in CSLCs; A549-CD133 + (H) and SKOV3- spheroid (I) cells were treated with different doses of EA for 48 h. <t>γH2AX</t> accumulation was detected through flow cytometry to evaluate the extent of DNA damage induced by EA. Representative contour plots obtained from flow cytometry analysis showing the percentage of γH2AX positive cells. Data was reanalyzed using FlowJo software. In all graphs, the results are represented as mean ± SD of triplicate experiments. ** P < 0.01, and *** P < 0.001.
H2ax Antibody, supplied by ZenBio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/h2ax antibody/product/ZenBio
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h2ax antibody - by Bioz Stars, 2026-04
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dapi/γh2ax/alms1  (Bioscientifica Ltd)
90
Bioscientifica Ltd dapi/γh2ax/alms1
EA treatment alters mitochondrial dynamics, leading to the accumulation of intracellular ROS and DNA damage. Western blot analysis of the protein levels of Mfn2, Drp1, total-ERK, and p-ERK in the (A) A2780 monolayer, (B) A2780 spheroids, and (C) SKOV3 spheroids cells; A549-CD133 + (D,E) and SKOV3- spheroids (F,G) were treated with different concentrations of EA (0, 5, 10, 25 μM) for 24 h. Intracellular ROS accumulation was determined using flow cytometry. Representative histograms and corresponding bar diagrams showing ROS level in CSLCs; A549-CD133 + (H) and SKOV3- spheroid (I) cells were treated with different doses of EA for 48 h. <t>γH2AX</t> accumulation was detected through flow cytometry to evaluate the extent of DNA damage induced by EA. Representative contour plots obtained from flow cytometry analysis showing the percentage of γH2AX positive cells. Data was reanalyzed using FlowJo software. In all graphs, the results are represented as mean ± SD of triplicate experiments. ** P < 0.01, and *** P < 0.001.
Dapi/γh2ax/Alms1, supplied by Bioscientifica Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dapi/γh2ax/alms1/product/Bioscientifica Ltd
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Image Search Results


EA treatment alters mitochondrial dynamics, leading to the accumulation of intracellular ROS and DNA damage. Western blot analysis of the protein levels of Mfn2, Drp1, total-ERK, and p-ERK in the (A) A2780 monolayer, (B) A2780 spheroids, and (C) SKOV3 spheroids cells; A549-CD133 + (D,E) and SKOV3- spheroids (F,G) were treated with different concentrations of EA (0, 5, 10, 25 μM) for 24 h. Intracellular ROS accumulation was determined using flow cytometry. Representative histograms and corresponding bar diagrams showing ROS level in CSLCs; A549-CD133 + (H) and SKOV3- spheroid (I) cells were treated with different doses of EA for 48 h. γH2AX accumulation was detected through flow cytometry to evaluate the extent of DNA damage induced by EA. Representative contour plots obtained from flow cytometry analysis showing the percentage of γH2AX positive cells. Data was reanalyzed using FlowJo software. In all graphs, the results are represented as mean ± SD of triplicate experiments. ** P < 0.01, and *** P < 0.001.

Journal: ACS Omega

Article Title: Ellagic Acid Induces DNA Damage and Apoptosis in Cancer Stem-like Cells and Overcomes Cisplatin Resistance

doi: 10.1021/acsomega.3c08819

Figure Lengend Snippet: EA treatment alters mitochondrial dynamics, leading to the accumulation of intracellular ROS and DNA damage. Western blot analysis of the protein levels of Mfn2, Drp1, total-ERK, and p-ERK in the (A) A2780 monolayer, (B) A2780 spheroids, and (C) SKOV3 spheroids cells; A549-CD133 + (D,E) and SKOV3- spheroids (F,G) were treated with different concentrations of EA (0, 5, 10, 25 μM) for 24 h. Intracellular ROS accumulation was determined using flow cytometry. Representative histograms and corresponding bar diagrams showing ROS level in CSLCs; A549-CD133 + (H) and SKOV3- spheroid (I) cells were treated with different doses of EA for 48 h. γH2AX accumulation was detected through flow cytometry to evaluate the extent of DNA damage induced by EA. Representative contour plots obtained from flow cytometry analysis showing the percentage of γH2AX positive cells. Data was reanalyzed using FlowJo software. In all graphs, the results are represented as mean ± SD of triplicate experiments. ** P < 0.01, and *** P < 0.001.

Article Snippet: Washing was done twice with 1× PBS and staining was performed using the γH2AX (Miltenyi Biotec cat. no. 130-130-829) antibody.

Techniques: Western Blot, Flow Cytometry, Software

Combinatorial treatment of EA and cisplatin impairs DNA damage repair in lung and ovarian CSLCs. The DNA repair kinetics study depicts the percent DNA damage accumulation following DMSO, EA, cisplatin, and EA + cisplatin treatment for 12 h followed by 3 h damage recovery in (A) A549-CD133 + and (B) SKOV3 spheroid cells. (C) The immunofluorescence staining shows the increasing accumulation of DNA damage in DMSO, EA, cisplatin, and EA + cisplatin-treated cells. The corresponding graph represents the mean fluorescence intensity of accumulated γH2AX. N=3, Bar, SD; * P < 0.05, ** P < 0.01, and *** P < 0.001.

Journal: ACS Omega

Article Title: Ellagic Acid Induces DNA Damage and Apoptosis in Cancer Stem-like Cells and Overcomes Cisplatin Resistance

doi: 10.1021/acsomega.3c08819

Figure Lengend Snippet: Combinatorial treatment of EA and cisplatin impairs DNA damage repair in lung and ovarian CSLCs. The DNA repair kinetics study depicts the percent DNA damage accumulation following DMSO, EA, cisplatin, and EA + cisplatin treatment for 12 h followed by 3 h damage recovery in (A) A549-CD133 + and (B) SKOV3 spheroid cells. (C) The immunofluorescence staining shows the increasing accumulation of DNA damage in DMSO, EA, cisplatin, and EA + cisplatin-treated cells. The corresponding graph represents the mean fluorescence intensity of accumulated γH2AX. N=3, Bar, SD; * P < 0.05, ** P < 0.01, and *** P < 0.001.

Article Snippet: Washing was done twice with 1× PBS and staining was performed using the γH2AX (Miltenyi Biotec cat. no. 130-130-829) antibody.

Techniques: Immunofluorescence, Staining, Fluorescence