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Image Search Results
Journal: Scientific Reports
Article Title: Copper(II)-binding equilibria in human blood
doi: 10.1038/s41598-020-62560-4
Figure Lengend Snippet: Separation of human serum proteins by SEC. 4 x diluted human serum (a), 10 μM Cu•CP ( b ), 10 μM HSA plus 10 μM Cu(II) ( c ), 2.5 μM α2M tetramer plus 10 μM Cu(II) ( d ), 10 μM HSA, 2.5 μM α2M tetramer plus 10 μM Cu(II) ( e ). Conditions: incubation buffer 50 mM HEPES, 50 mM NaCl, pH 7.4; LC-ICP MS: column - 10 × 300 mm Superdex 200; elution buffer 200 mM NH 4 NO 3 , pH 7.4; flow rate 0.4 ml/min; injection volume 10 μl; UV at 280 nm (left axis and black lines) and Cu-63 (right axis and red line), monitored by ICP MS. Figure was created by program Origin 9 Pro ( https://www.originlab.com/ ).
Article Snippet: The experiments were carried out with two preparations of normal (“slow form”) human plasma α2M (from Sigma and Athens Research) as well as with the so-called “fast form” of
Techniques: Incubation, Injection