zvad.fmk Search Results


benzyloxycarbonyl-val–ala–asp zvadfmk zvad  (Promega)
90
Promega benzyloxycarbonyl-val–ala–asp zvadfmk zvad
Benzyloxycarbonyl Val–Ala–Asp Zvadfmk Zvad, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/benzyloxycarbonyl-val–ala–asp zvadfmk zvad/product/Promega
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benzyloxycarbonyl-val–ala–asp zvadfmk zvad - by Bioz Stars, 2026-02
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carbobenzoxy-valylalanyl-aspartyl-[o-methyl]-fluoromethylketone (z-vad-fmk  (Becton Dickinson)
90
Becton Dickinson carbobenzoxy-valylalanyl-aspartyl-[o-methyl]-fluoromethylketone (z-vad-fmk
Carbobenzoxy Valylalanyl Aspartyl [O Methyl] Fluoromethylketone (Z Vad Fmk, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/carbobenzoxy-valylalanyl-aspartyl-[o-methyl]-fluoromethylketone (z-vad-fmk/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
carbobenzoxy-valylalanyl-aspartyl-[o-methyl]-fluoromethylketone (z-vad-fmk - by Bioz Stars, 2026-02
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n-benzyl-oxycarbonyl-val-ala-asp-fluoromethylketone (z-vad-fmk  (Kamiya)
90
Kamiya n-benzyl-oxycarbonyl-val-ala-asp-fluoromethylketone (z-vad-fmk
N Benzyl Oxycarbonyl Val Ala Asp Fluoromethylketone (Z Vad Fmk, supplied by Kamiya, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/n-benzyl-oxycarbonyl-val-ala-asp-fluoromethylketone (z-vad-fmk/product/Kamiya
Average 90 stars, based on 1 article reviews
n-benzyl-oxycarbonyl-val-ala-asp-fluoromethylketone (z-vad-fmk - by Bioz Stars, 2026-02
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zvad.fmk  (Avantor)
90
Avantor zvad.fmk
a Prophylactic vaccination model assessing the immunogenicity of ferroptosis. ML162-induced ferroptosis (5 μM, 14 h), in comparison with apoptosis (1000 IU/ml TNF + 10 μM TAK1i, 14 h) and necroptosis (1000 IU/ml TNF + 10 μM TAK1i + 10 μM <t>zVAD.fmk,</t> 24 h). OVA-expressing non-tumorigenic MEF cells (BM1-OVA) were used as a vaccine and live ovalbumin expressing melanoma cells (B16-OVA) were used as challenge. Kaplan–Meier curves represent the effectiveness of ferroptotic cells in preventing the tumor growth at the challenge site. Data were analyzed by Kaplan–Meier simple survival analysis. b Tumor size of B16-OVA derived melanoma after vaccination with BM1-OVA cells. Data presented as mean ± SEM. c Scheme of the therapeutic vaccination experiment. Conventional dendritic cells type 1 (cDC1) carrying ferroptotic or necroptotic cargo were intradermally injected in melanoma tumor-bearing mice. d B16-OVA tumor size progression of animals receiving either cDC1 with ferroptotic (5 μM, 14 h) or necroptotic (1000 IU/ml TNF + 10 μM TAK1i + 10 μM zVAD.fmk, 24 h) cargo. Data presented as mean ± SEM. Statistical significance was determined by two-sided t-test on each day of measurement. e Comparison of euthanasia time determined by the size of the tumor. Kaplan–Meier curves show the time of euthanasia, Data analyzed by Kaplan–Meier simple survival analysis. f Prophylactic vaccination model using either Mitoxantrone-treated MCA205 cells (1 μM, 24 h) or Mitoxantrone-treated MCA205 mixed with ML162-killed cells (0.5 µM, 14 h). Kaplan–Meier curves show the percentage of tumor-free mice after the challenge with live cancer cells. Data were analyzed by Kaplan–Meier simple survival analysis.
Zvad.Fmk, supplied by Avantor, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/zvad.fmk/product/Avantor
Average 90 stars, based on 1 article reviews
zvad.fmk - by Bioz Stars, 2026-02
90/100 stars
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tnfa/ carbobenzoxyvalyl-alanyl-aspartyl-[o-methyl]-fluoromethylketone (zvad.fmk)/smac mimetic  (SMAC Corp)
90
SMAC Corp tnfa/ carbobenzoxyvalyl-alanyl-aspartyl-[o-methyl]-fluoromethylketone (zvad.fmk)/smac mimetic
a Prophylactic vaccination model assessing the immunogenicity of ferroptosis. ML162-induced ferroptosis (5 μM, 14 h), in comparison with apoptosis (1000 IU/ml TNF + 10 μM TAK1i, 14 h) and necroptosis (1000 IU/ml TNF + 10 μM TAK1i + 10 μM <t>zVAD.fmk,</t> 24 h). OVA-expressing non-tumorigenic MEF cells (BM1-OVA) were used as a vaccine and live ovalbumin expressing melanoma cells (B16-OVA) were used as challenge. Kaplan–Meier curves represent the effectiveness of ferroptotic cells in preventing the tumor growth at the challenge site. Data were analyzed by Kaplan–Meier simple survival analysis. b Tumor size of B16-OVA derived melanoma after vaccination with BM1-OVA cells. Data presented as mean ± SEM. c Scheme of the therapeutic vaccination experiment. Conventional dendritic cells type 1 (cDC1) carrying ferroptotic or necroptotic cargo were intradermally injected in melanoma tumor-bearing mice. d B16-OVA tumor size progression of animals receiving either cDC1 with ferroptotic (5 μM, 14 h) or necroptotic (1000 IU/ml TNF + 10 μM TAK1i + 10 μM zVAD.fmk, 24 h) cargo. Data presented as mean ± SEM. Statistical significance was determined by two-sided t-test on each day of measurement. e Comparison of euthanasia time determined by the size of the tumor. Kaplan–Meier curves show the time of euthanasia, Data analyzed by Kaplan–Meier simple survival analysis. f Prophylactic vaccination model using either Mitoxantrone-treated MCA205 cells (1 μM, 24 h) or Mitoxantrone-treated MCA205 mixed with ML162-killed cells (0.5 µM, 14 h). Kaplan–Meier curves show the percentage of tumor-free mice after the challenge with live cancer cells. Data were analyzed by Kaplan–Meier simple survival analysis.
Tnfa/ Carbobenzoxyvalyl Alanyl Aspartyl [O Methyl] Fluoromethylketone (Zvad.Fmk)/Smac Mimetic, supplied by SMAC Corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tnfa/ carbobenzoxyvalyl-alanyl-aspartyl-[o-methyl]-fluoromethylketone (zvad.fmk)/smac mimetic/product/SMAC Corp
Average 90 stars, based on 1 article reviews
tnfa/ carbobenzoxyvalyl-alanyl-aspartyl-[o-methyl]-fluoromethylketone (zvad.fmk)/smac mimetic - by Bioz Stars, 2026-02
90/100 stars
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apoptosis inhibitor zvad-fmk  (SCAPS GmbH)
90
SCAPS GmbH apoptosis inhibitor zvad-fmk
a Prophylactic vaccination model assessing the immunogenicity of ferroptosis. ML162-induced ferroptosis (5 μM, 14 h), in comparison with apoptosis (1000 IU/ml TNF + 10 μM TAK1i, 14 h) and necroptosis (1000 IU/ml TNF + 10 μM TAK1i + 10 μM <t>zVAD.fmk,</t> 24 h). OVA-expressing non-tumorigenic MEF cells (BM1-OVA) were used as a vaccine and live ovalbumin expressing melanoma cells (B16-OVA) were used as challenge. Kaplan–Meier curves represent the effectiveness of ferroptotic cells in preventing the tumor growth at the challenge site. Data were analyzed by Kaplan–Meier simple survival analysis. b Tumor size of B16-OVA derived melanoma after vaccination with BM1-OVA cells. Data presented as mean ± SEM. c Scheme of the therapeutic vaccination experiment. Conventional dendritic cells type 1 (cDC1) carrying ferroptotic or necroptotic cargo were intradermally injected in melanoma tumor-bearing mice. d B16-OVA tumor size progression of animals receiving either cDC1 with ferroptotic (5 μM, 14 h) or necroptotic (1000 IU/ml TNF + 10 μM TAK1i + 10 μM zVAD.fmk, 24 h) cargo. Data presented as mean ± SEM. Statistical significance was determined by two-sided t-test on each day of measurement. e Comparison of euthanasia time determined by the size of the tumor. Kaplan–Meier curves show the time of euthanasia, Data analyzed by Kaplan–Meier simple survival analysis. f Prophylactic vaccination model using either Mitoxantrone-treated MCA205 cells (1 μM, 24 h) or Mitoxantrone-treated MCA205 mixed with ML162-killed cells (0.5 µM, 14 h). Kaplan–Meier curves show the percentage of tumor-free mice after the challenge with live cancer cells. Data were analyzed by Kaplan–Meier simple survival analysis.
Apoptosis Inhibitor Zvad Fmk, supplied by SCAPS GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/apoptosis inhibitor zvad-fmk/product/SCAPS GmbH
Average 90 stars, based on 1 article reviews
apoptosis inhibitor zvad-fmk - by Bioz Stars, 2026-02
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zvad.fmk  (Bachem)
90
Bachem zvad.fmk
a Prophylactic vaccination model assessing the immunogenicity of ferroptosis. ML162-induced ferroptosis (5 μM, 14 h), in comparison with apoptosis (1000 IU/ml TNF + 10 μM TAK1i, 14 h) and necroptosis (1000 IU/ml TNF + 10 μM TAK1i + 10 μM <t>zVAD.fmk,</t> 24 h). OVA-expressing non-tumorigenic MEF cells (BM1-OVA) were used as a vaccine and live ovalbumin expressing melanoma cells (B16-OVA) were used as challenge. Kaplan–Meier curves represent the effectiveness of ferroptotic cells in preventing the tumor growth at the challenge site. Data were analyzed by Kaplan–Meier simple survival analysis. b Tumor size of B16-OVA derived melanoma after vaccination with BM1-OVA cells. Data presented as mean ± SEM. c Scheme of the therapeutic vaccination experiment. Conventional dendritic cells type 1 (cDC1) carrying ferroptotic or necroptotic cargo were intradermally injected in melanoma tumor-bearing mice. d B16-OVA tumor size progression of animals receiving either cDC1 with ferroptotic (5 μM, 14 h) or necroptotic (1000 IU/ml TNF + 10 μM TAK1i + 10 μM zVAD.fmk, 24 h) cargo. Data presented as mean ± SEM. Statistical significance was determined by two-sided t-test on each day of measurement. e Comparison of euthanasia time determined by the size of the tumor. Kaplan–Meier curves show the time of euthanasia, Data analyzed by Kaplan–Meier simple survival analysis. f Prophylactic vaccination model using either Mitoxantrone-treated MCA205 cells (1 μM, 24 h) or Mitoxantrone-treated MCA205 mixed with ML162-killed cells (0.5 µM, 14 h). Kaplan–Meier curves show the percentage of tumor-free mice after the challenge with live cancer cells. Data were analyzed by Kaplan–Meier simple survival analysis.
Zvad.Fmk, supplied by Bachem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/zvad.fmk/product/Bachem
Average 90 stars, based on 1 article reviews
zvad.fmk - by Bioz Stars, 2026-02
90/100 stars
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zvad-fmk  (Enzo Biochem)
90
Enzo Biochem zvad-fmk
a Prophylactic vaccination model assessing the immunogenicity of ferroptosis. ML162-induced ferroptosis (5 μM, 14 h), in comparison with apoptosis (1000 IU/ml TNF + 10 μM TAK1i, 14 h) and necroptosis (1000 IU/ml TNF + 10 μM TAK1i + 10 μM <t>zVAD.fmk,</t> 24 h). OVA-expressing non-tumorigenic MEF cells (BM1-OVA) were used as a vaccine and live ovalbumin expressing melanoma cells (B16-OVA) were used as challenge. Kaplan–Meier curves represent the effectiveness of ferroptotic cells in preventing the tumor growth at the challenge site. Data were analyzed by Kaplan–Meier simple survival analysis. b Tumor size of B16-OVA derived melanoma after vaccination with BM1-OVA cells. Data presented as mean ± SEM. c Scheme of the therapeutic vaccination experiment. Conventional dendritic cells type 1 (cDC1) carrying ferroptotic or necroptotic cargo were intradermally injected in melanoma tumor-bearing mice. d B16-OVA tumor size progression of animals receiving either cDC1 with ferroptotic (5 μM, 14 h) or necroptotic (1000 IU/ml TNF + 10 μM TAK1i + 10 μM zVAD.fmk, 24 h) cargo. Data presented as mean ± SEM. Statistical significance was determined by two-sided t-test on each day of measurement. e Comparison of euthanasia time determined by the size of the tumor. Kaplan–Meier curves show the time of euthanasia, Data analyzed by Kaplan–Meier simple survival analysis. f Prophylactic vaccination model using either Mitoxantrone-treated MCA205 cells (1 μM, 24 h) or Mitoxantrone-treated MCA205 mixed with ML162-killed cells (0.5 µM, 14 h). Kaplan–Meier curves show the percentage of tumor-free mice after the challenge with live cancer cells. Data were analyzed by Kaplan–Meier simple survival analysis.
Zvad Fmk, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/zvad-fmk/product/Enzo Biochem
Average 90 stars, based on 1 article reviews
zvad-fmk - by Bioz Stars, 2026-02
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zvad  (Merck KGaA)
90
Merck KGaA zvad
a Prophylactic vaccination model assessing the immunogenicity of ferroptosis. ML162-induced ferroptosis (5 μM, 14 h), in comparison with apoptosis (1000 IU/ml TNF + 10 μM TAK1i, 14 h) and necroptosis (1000 IU/ml TNF + 10 μM TAK1i + 10 μM <t>zVAD.fmk,</t> 24 h). OVA-expressing non-tumorigenic MEF cells (BM1-OVA) were used as a vaccine and live ovalbumin expressing melanoma cells (B16-OVA) were used as challenge. Kaplan–Meier curves represent the effectiveness of ferroptotic cells in preventing the tumor growth at the challenge site. Data were analyzed by Kaplan–Meier simple survival analysis. b Tumor size of B16-OVA derived melanoma after vaccination with BM1-OVA cells. Data presented as mean ± SEM. c Scheme of the therapeutic vaccination experiment. Conventional dendritic cells type 1 (cDC1) carrying ferroptotic or necroptotic cargo were intradermally injected in melanoma tumor-bearing mice. d B16-OVA tumor size progression of animals receiving either cDC1 with ferroptotic (5 μM, 14 h) or necroptotic (1000 IU/ml TNF + 10 μM TAK1i + 10 μM zVAD.fmk, 24 h) cargo. Data presented as mean ± SEM. Statistical significance was determined by two-sided t-test on each day of measurement. e Comparison of euthanasia time determined by the size of the tumor. Kaplan–Meier curves show the time of euthanasia, Data analyzed by Kaplan–Meier simple survival analysis. f Prophylactic vaccination model using either Mitoxantrone-treated MCA205 cells (1 μM, 24 h) or Mitoxantrone-treated MCA205 mixed with ML162-killed cells (0.5 µM, 14 h). Kaplan–Meier curves show the percentage of tumor-free mice after the challenge with live cancer cells. Data were analyzed by Kaplan–Meier simple survival analysis.
Zvad, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/zvad/product/Merck KGaA
Average 90 stars, based on 1 article reviews
zvad - by Bioz Stars, 2026-02
90/100 stars
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zvad.fmk  (Becton Dickinson)
90
Becton Dickinson zvad.fmk
a Prophylactic vaccination model assessing the immunogenicity of ferroptosis. ML162-induced ferroptosis (5 μM, 14 h), in comparison with apoptosis (1000 IU/ml TNF + 10 μM TAK1i, 14 h) and necroptosis (1000 IU/ml TNF + 10 μM TAK1i + 10 μM <t>zVAD.fmk,</t> 24 h). OVA-expressing non-tumorigenic MEF cells (BM1-OVA) were used as a vaccine and live ovalbumin expressing melanoma cells (B16-OVA) were used as challenge. Kaplan–Meier curves represent the effectiveness of ferroptotic cells in preventing the tumor growth at the challenge site. Data were analyzed by Kaplan–Meier simple survival analysis. b Tumor size of B16-OVA derived melanoma after vaccination with BM1-OVA cells. Data presented as mean ± SEM. c Scheme of the therapeutic vaccination experiment. Conventional dendritic cells type 1 (cDC1) carrying ferroptotic or necroptotic cargo were intradermally injected in melanoma tumor-bearing mice. d B16-OVA tumor size progression of animals receiving either cDC1 with ferroptotic (5 μM, 14 h) or necroptotic (1000 IU/ml TNF + 10 μM TAK1i + 10 μM zVAD.fmk, 24 h) cargo. Data presented as mean ± SEM. Statistical significance was determined by two-sided t-test on each day of measurement. e Comparison of euthanasia time determined by the size of the tumor. Kaplan–Meier curves show the time of euthanasia, Data analyzed by Kaplan–Meier simple survival analysis. f Prophylactic vaccination model using either Mitoxantrone-treated MCA205 cells (1 μM, 24 h) or Mitoxantrone-treated MCA205 mixed with ML162-killed cells (0.5 µM, 14 h). Kaplan–Meier curves show the percentage of tumor-free mice after the challenge with live cancer cells. Data were analyzed by Kaplan–Meier simple survival analysis.
Zvad.Fmk, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/zvad.fmk/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
zvad.fmk - by Bioz Stars, 2026-02
90/100 stars
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zvad-fmk  (Adooq Bioscience LLC)
90
Adooq Bioscience LLC zvad-fmk
a Prophylactic vaccination model assessing the immunogenicity of ferroptosis. ML162-induced ferroptosis (5 μM, 14 h), in comparison with apoptosis (1000 IU/ml TNF + 10 μM TAK1i, 14 h) and necroptosis (1000 IU/ml TNF + 10 μM TAK1i + 10 μM <t>zVAD.fmk,</t> 24 h). OVA-expressing non-tumorigenic MEF cells (BM1-OVA) were used as a vaccine and live ovalbumin expressing melanoma cells (B16-OVA) were used as challenge. Kaplan–Meier curves represent the effectiveness of ferroptotic cells in preventing the tumor growth at the challenge site. Data were analyzed by Kaplan–Meier simple survival analysis. b Tumor size of B16-OVA derived melanoma after vaccination with BM1-OVA cells. Data presented as mean ± SEM. c Scheme of the therapeutic vaccination experiment. Conventional dendritic cells type 1 (cDC1) carrying ferroptotic or necroptotic cargo were intradermally injected in melanoma tumor-bearing mice. d B16-OVA tumor size progression of animals receiving either cDC1 with ferroptotic (5 μM, 14 h) or necroptotic (1000 IU/ml TNF + 10 μM TAK1i + 10 μM zVAD.fmk, 24 h) cargo. Data presented as mean ± SEM. Statistical significance was determined by two-sided t-test on each day of measurement. e Comparison of euthanasia time determined by the size of the tumor. Kaplan–Meier curves show the time of euthanasia, Data analyzed by Kaplan–Meier simple survival analysis. f Prophylactic vaccination model using either Mitoxantrone-treated MCA205 cells (1 μM, 24 h) or Mitoxantrone-treated MCA205 mixed with ML162-killed cells (0.5 µM, 14 h). Kaplan–Meier curves show the percentage of tumor-free mice after the challenge with live cancer cells. Data were analyzed by Kaplan–Meier simple survival analysis.
Zvad Fmk, supplied by Adooq Bioscience LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/zvad-fmk/product/Adooq Bioscience LLC
Average 90 stars, based on 1 article reviews
zvad-fmk - by Bioz Stars, 2026-02
90/100 stars
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zvad-fmk inhibitor  (Cayman Chemical)
90
Cayman Chemical zvad-fmk inhibitor
a Prophylactic vaccination model assessing the immunogenicity of ferroptosis. ML162-induced ferroptosis (5 μM, 14 h), in comparison with apoptosis (1000 IU/ml TNF + 10 μM TAK1i, 14 h) and necroptosis (1000 IU/ml TNF + 10 μM TAK1i + 10 μM <t>zVAD.fmk,</t> 24 h). OVA-expressing non-tumorigenic MEF cells (BM1-OVA) were used as a vaccine and live ovalbumin expressing melanoma cells (B16-OVA) were used as challenge. Kaplan–Meier curves represent the effectiveness of ferroptotic cells in preventing the tumor growth at the challenge site. Data were analyzed by Kaplan–Meier simple survival analysis. b Tumor size of B16-OVA derived melanoma after vaccination with BM1-OVA cells. Data presented as mean ± SEM. c Scheme of the therapeutic vaccination experiment. Conventional dendritic cells type 1 (cDC1) carrying ferroptotic or necroptotic cargo were intradermally injected in melanoma tumor-bearing mice. d B16-OVA tumor size progression of animals receiving either cDC1 with ferroptotic (5 μM, 14 h) or necroptotic (1000 IU/ml TNF + 10 μM TAK1i + 10 μM zVAD.fmk, 24 h) cargo. Data presented as mean ± SEM. Statistical significance was determined by two-sided t-test on each day of measurement. e Comparison of euthanasia time determined by the size of the tumor. Kaplan–Meier curves show the time of euthanasia, Data analyzed by Kaplan–Meier simple survival analysis. f Prophylactic vaccination model using either Mitoxantrone-treated MCA205 cells (1 μM, 24 h) or Mitoxantrone-treated MCA205 mixed with ML162-killed cells (0.5 µM, 14 h). Kaplan–Meier curves show the percentage of tumor-free mice after the challenge with live cancer cells. Data were analyzed by Kaplan–Meier simple survival analysis.
Zvad Fmk Inhibitor, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/zvad-fmk inhibitor/product/Cayman Chemical
Average 90 stars, based on 1 article reviews
zvad-fmk inhibitor - by Bioz Stars, 2026-02
90/100 stars
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Image Search Results


a Prophylactic vaccination model assessing the immunogenicity of ferroptosis. ML162-induced ferroptosis (5 μM, 14 h), in comparison with apoptosis (1000 IU/ml TNF + 10 μM TAK1i, 14 h) and necroptosis (1000 IU/ml TNF + 10 μM TAK1i + 10 μM zVAD.fmk, 24 h). OVA-expressing non-tumorigenic MEF cells (BM1-OVA) were used as a vaccine and live ovalbumin expressing melanoma cells (B16-OVA) were used as challenge. Kaplan–Meier curves represent the effectiveness of ferroptotic cells in preventing the tumor growth at the challenge site. Data were analyzed by Kaplan–Meier simple survival analysis. b Tumor size of B16-OVA derived melanoma after vaccination with BM1-OVA cells. Data presented as mean ± SEM. c Scheme of the therapeutic vaccination experiment. Conventional dendritic cells type 1 (cDC1) carrying ferroptotic or necroptotic cargo were intradermally injected in melanoma tumor-bearing mice. d B16-OVA tumor size progression of animals receiving either cDC1 with ferroptotic (5 μM, 14 h) or necroptotic (1000 IU/ml TNF + 10 μM TAK1i + 10 μM zVAD.fmk, 24 h) cargo. Data presented as mean ± SEM. Statistical significance was determined by two-sided t-test on each day of measurement. e Comparison of euthanasia time determined by the size of the tumor. Kaplan–Meier curves show the time of euthanasia, Data analyzed by Kaplan–Meier simple survival analysis. f Prophylactic vaccination model using either Mitoxantrone-treated MCA205 cells (1 μM, 24 h) or Mitoxantrone-treated MCA205 mixed with ML162-killed cells (0.5 µM, 14 h). Kaplan–Meier curves show the percentage of tumor-free mice after the challenge with live cancer cells. Data were analyzed by Kaplan–Meier simple survival analysis.

Journal: Nature Communications

Article Title: Cancer cells dying from ferroptosis impede dendritic cell-mediated anti-tumor immunity

doi: 10.1038/s41467-022-31218-2

Figure Lengend Snippet: a Prophylactic vaccination model assessing the immunogenicity of ferroptosis. ML162-induced ferroptosis (5 μM, 14 h), in comparison with apoptosis (1000 IU/ml TNF + 10 μM TAK1i, 14 h) and necroptosis (1000 IU/ml TNF + 10 μM TAK1i + 10 μM zVAD.fmk, 24 h). OVA-expressing non-tumorigenic MEF cells (BM1-OVA) were used as a vaccine and live ovalbumin expressing melanoma cells (B16-OVA) were used as challenge. Kaplan–Meier curves represent the effectiveness of ferroptotic cells in preventing the tumor growth at the challenge site. Data were analyzed by Kaplan–Meier simple survival analysis. b Tumor size of B16-OVA derived melanoma after vaccination with BM1-OVA cells. Data presented as mean ± SEM. c Scheme of the therapeutic vaccination experiment. Conventional dendritic cells type 1 (cDC1) carrying ferroptotic or necroptotic cargo were intradermally injected in melanoma tumor-bearing mice. d B16-OVA tumor size progression of animals receiving either cDC1 with ferroptotic (5 μM, 14 h) or necroptotic (1000 IU/ml TNF + 10 μM TAK1i + 10 μM zVAD.fmk, 24 h) cargo. Data presented as mean ± SEM. Statistical significance was determined by two-sided t-test on each day of measurement. e Comparison of euthanasia time determined by the size of the tumor. Kaplan–Meier curves show the time of euthanasia, Data analyzed by Kaplan–Meier simple survival analysis. f Prophylactic vaccination model using either Mitoxantrone-treated MCA205 cells (1 μM, 24 h) or Mitoxantrone-treated MCA205 mixed with ML162-killed cells (0.5 µM, 14 h). Kaplan–Meier curves show the percentage of tumor-free mice after the challenge with live cancer cells. Data were analyzed by Kaplan–Meier simple survival analysis.

Article Snippet: Cell death inhibitors zVAD.fmk (#BACE4026865.0005, VWR International, Belgium) and Nec-1s (synthesized by the Laboratory of Medicinal Chemistry; University of Antwerp, Belgium) were used at 10 μM, Fer1 (#M60042-2s, Xcess Biosciences, USA) was used at 0,5 μM and DFO (#D-9533, Sigma-Aldrich, USA) at 50 μM.

Techniques: Expressing, Derivative Assay, Injection