Journal: BMJ Neurology Open

Article Title: Altered amyloid plasma profile in patients with disabling headaches after SARS-CoV-2 infection and vaccination

doi: 10.1136/bmjno-2024-001013

Figure Lengend Snippet: Plasma levels of (A) amyloid precursor protein (APP), (B) cathepsin L, (C) pregnancy zone protein (PZP) and (D) serum amyloid A (SAA1) in healthy controls (HC) (n=16), participants with persistent headache after SARS-CoV-2 vaccine (COvax) (n=31) and participants with persistent headache after COVID-19 ( C19 ) (n=29).

Article Snippet: Plasma levels of APP (Cat# DY850), PZP (Cat# DY8280-05), CTSL (Cat# DY952) and SAA1 (Cat# DY3019-05) were measured by ELISA using commercially available antibodies (R&D Systems, Minneapolis, Minnesota, USA) in a 384-format using a combination of a SELMA pipetting robot (Analytik Jena AG, Jena, Germany) and a BioTek dispenser/washer (BioTek Instruments, Winooski, VT).

Techniques: Clinical Proteomics

Journal: Cell reports

Article Title: Pleiotrophin regulates presynaptic assembly and function through heparan sulfate-dependent binding to neurexin1

doi: 10.1016/j.celrep.2026.117827

Figure Lengend Snippet:

Article Snippet: Anti-RIM , Synaptic Systems , Cat# 140205; RRID:AB_2631216.

Techniques: Virus, Recombinant, Magnetic Beads, Mass Spectrometry, shRNA, Sequencing, Software

Journal: Frontiers in Immunology

Article Title: TMT-Based Quantitative Proteomics Analysis of Synovial Fluid-Derived Exosomes in Inflammatory Arthritis

doi: 10.3389/fimmu.2022.800902

Figure Lengend Snippet: Verification of exosome-derived PZP in SF by ELISA. The ELISA kits were performed to detect the PZP level of SF-derived exosomes in gout, axSpA, OA, and RA. *P value < 0.05 vs. OA groups.

Article Snippet: The ELISA Kits (CSB-EL019131HU, CUSABIO BIOTECH) were performed to detect the pregnancy zone protein (PZP) level of SF-derived exosomes in all groups, following the manufacturer’s instructions.

Techniques: Derivative Assay, Enzyme-linked Immunosorbent Assay

Journal: Clinical Proteomics

Article Title: iTRAQ plasma proteomics analysis for candidate biomarkers of type 2 incipient diabetic nephropathy

doi: 10.1186/s12014-019-9253-1

Figure Lengend Snippet: MS/MS spectrum for iTRAQ quantification. A representative MS/MS spectrum indicated important peptide segments for gelsolin, collectin-11, PTPRJ, AKAP-7, pregnancy zone protein and transthyretin. iTRAQ tags showed the relative expression of the six proteins individually in the plasma of type 2 incipient diabetic nephropathy patients compared to the control

Article Snippet: Primary antibodies against gelsolin (Abcam, #109014), transthyretin (Abcam, #92469), pregnancy zone protein (Proteintech, #21742), A-kinase anchor protein 7 (Proteintech, #12591), PTPRJ (Proteintech, #55123), and collectin-11 (Proteintech, #15269) were incubated overnight, followed by the appropriate horseradish peroxidase-conjugated secondary antibodies.

Techniques: Tandem Mass Spectroscopy, Multiplex sample analysis, Expressing, Clinical Proteomics, Control

Journal: Clinical Proteomics

Article Title: iTRAQ plasma proteomics analysis for candidate biomarkers of type 2 incipient diabetic nephropathy

doi: 10.1186/s12014-019-9253-1

Figure Lengend Snippet: Western blot confirmation of six selected proteins. Representative WB images for six proteins, including gelsolin, collectin-11, PTPRJ and AKAP-7, pregnancy zone protein and transthyretin. Data are expressed as the mean using GraphPad Prism. ****p < 0.0001. Controls (n = 62), IDN patients (n = 62)

Article Snippet: Primary antibodies against gelsolin (Abcam, #109014), transthyretin (Abcam, #92469), pregnancy zone protein (Proteintech, #21742), A-kinase anchor protein 7 (Proteintech, #12591), PTPRJ (Proteintech, #55123), and collectin-11 (Proteintech, #15269) were incubated overnight, followed by the appropriate horseradish peroxidase-conjugated secondary antibodies.

Techniques: Western Blot

Journal: eLife

Article Title: A dual role for Ca v 1.4 Ca 2+ channels in the molecular and structural organization of the rod photoreceptor synapse

doi: 10.7554/eLife.62184

Figure Lengend Snippet: ( A ) Confocal micrographs of the OPL of WT, G369i KI and Ca v 1.4 KO mice labeled with antibodies against CtBP2 to mark ribbons (arrows). Arrowheads depict spheres resembling immature ribbons. ( B ) Cumulative frequency of rod ribbon lengths in WT, G369i KI, and Ca v 1.4 KO. Ribbon states less than and greater than 0.95 µm were classified as spheres and ribbons, respectively. ( C , D ) Violin plots of rod ribbon lengths ( C ) and sphericity ( D ) obtained from dataset in B . **** p<0.001 by one-way ANOVA with Tukey’s multiple comparison post-hoc test. WT, n = 1264 ribbons; KO, n = 348 ribbons; G369i KI, n = 1322 ribbons. Data were collected from the retinas of at least six individual mice per genotype. ( E ) Schematic of a rod active zone and associated proteins. SV, synaptic vesicle. ( F–I ) Confocal micrographs (maximum z-projections) of the OPL of WT, G369i KI and Ca v 1.4 KO mice double-labeled with antibodies against CtBP2 and PSD95 ( F ), Ribeye and β-dystroglycan (β-Dys), ( G ) CtBP2 and Bassoon ( H ) and CtBP2 and RIM2 ( I ). Arrows and arrowheads depict ribbons and spheres, respectively. Scale bars, 5 μm in A , 2 μm in F - I . Figure 2—source data 1. The image archive contains all images used in quantitative analyses in . The data were exported as 8-bit ‘tif’ files (1600 × 1600 pixels). Values obtained for individual data points (and outlier analysis) for summary graphs in are contained in ‘.xlsx’ files.

Article Snippet: RIM2 , rabbit , Synaptic Systems , 140–103 , AB_887776.

Techniques: Labeling, Comparison

Journal: eLife

Article Title: A dual role for Ca v 1.4 Ca 2+ channels in the molecular and structural organization of the rod photoreceptor synapse

doi: 10.7554/eLife.62184

Figure Lengend Snippet: Antibodies used in this study.

Article Snippet: RIM2 , rabbit , Synaptic Systems , 140–103 , AB_887776.

Techniques:

Journal: eLife

Article Title: Functional hierarchy among different Rab27 effectors involved in secretory granule exocytosis

doi: 10.7554/eLife.82821

Figure Lengend Snippet:

Article Snippet: Antibody , Anti-RIM1/2 (rabbit polyclonal) , Synaptic Systems , Cat#: 140213; RRID: AB_2832237 , WB (1:2000).

Techniques: Transfection, Construct, Isolation, Recombinant, Plasmid Preparation, Software, Western Blot, Mass Spectrometry