Journal: eLife

Article Title: Single-cell transcriptomics identifies altered neutrophil dynamics and accentuated T-cell cytotoxicity in tobacco-flavored e-cigarette-exposed mouse lungs

doi: 10.7554/eLife.106380

Figure Lengend Snippet: The levels of pro-inflammatory cytokines/chemokines in the lung digests from experimental (PG:VG, PG:VG + Nic, fruit, menthol, and tobacco) and control (air) were assessed using multianalyte assay. The results obtained were plotted as a heatmap with the z -scores represented between the scale of orange (low) to blue (high) ( A ). Heatmap showing the fold changes in the expression of commonly dysregulated genes in the myeloid and lymphoid clusters in mouse lungs exposed to flavored e-cig aerosols as compared to ambient air as determined after DESeq2 analyses ( B ). CNET plot results showing the pathways regulated by the common differentially expressed genes (DEGs) (across all cell types) on acute (5-day) exposure to differently flavored (fruit, menthol, and tobacco) e-cig aerosols in C57BL/6J mouse lungs ( C ). Data is representative of n = 2/sex/group. Figure 9—figure supplement 1—source data 1. Z -score values of the levels of cytokine/chemokine obtained in per mg of protein from mouse lung tissue samples following 5-day nose-only exposure to PG:VG, PG:VG + Nic, fruit, menthol, or tobacco-flavored e-cig aerosols as plotted in . Figure 9—figure supplement 1—source data 2. Z -score values of the gene expression of commonly dysregulated genes in the mouse lung tissue samples following 5-day nose-only exposure to PG:VG, fruit, menthol, or tobacco-flavored e-cig aerosols as compared to air controls as plotted in .

Article Snippet: We used a multiplex assay to determine the levels of cytokine/chemokine in the lung homogenates from control and e-cig aerosol exposed mouse lungs using commercially available Bio-Plex Pro Mouse Chemokine Assay (Cat# 12009159, Bio-Rad, Hercules, CA) per the manufacturer’s instructions.

Techniques: Control, Expressing, Gene Expression