Journal: PLoS Pathogens

Article Title: The Production of Monokaryotic Hyphae by Cryptococcus neoformans Can Be Induced by High Temperature Arrest of the Cell Cycle and Is Independent of Same-Sex Mating

doi: 10.1371/journal.ppat.1003335

Figure Lengend Snippet: JEC-21 cells were cultured in YPD broth plus different concentrations of nocodazole at 30°C for 24 hrs, then dropped onto filament agar and incubated at 25°C for 5 days. (A) Qualitative agar assay for monokaryotic fruiting. Nocodazole concentrations consisted of a.) 0 µM, b.) 0.075 µM, c.) 0.15 µM, d.) 0.30 µM, e.) 0.60 µM, f.) 1.20 µM. Hyphal production begins at nocodazole concentrations ≥0.15 µM. Bar = 0.5 mm. (B) Quantitative agar assay for monokaryotic fruiting. Concentrations of nocodazole are indicated. The percent of colonies undergoing monokaryotic fruiting was calculated from the total colonies that grew out on the agar plates. Plates were assayed in triplicate.

Article Snippet: JEC-21 cells were added to 1.5 ml nocodazole-YPD broth in 15 ml snap cap tubes (BD Biosciences, Franklin Lakes, NJ), at a final concentration of 1×10 6 cells/ml.

Techniques: Cell Culture, Incubation